Restriction map for pF1KB5463
 (Linear) (Six-base) MAPSORT of: KIBB5463.seq  Check: 1231  from: 1  to: 864



KIBB5463.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      96     864 
   Size:         96     768
 
AccI GT'mk_AC

Cuts at:      0     722     864 
   Size:        722     142
 
AfeI AGC'GCT

Cuts at:      0     430     864 
   Size:        430     434
 
AflIII A'CryG_T

Cuts at:      0     641     864 
   Size:        641     223
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     310     342     864 
   Size:        310      32     522

  Fragments arranged by size: 

                 522     310      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     573     864 
   Size:        573     291
 
AscI GG'CGCG_CC

Cuts at:      0     781     864 
   Size:        781      83
 
BamHI G'GATC_C

Cuts at:      0     341     864 
   Size:        341     523
 
BanII G_rGCy'C

Cuts at:      0      23     864 
   Size:         23     841
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     214     256     355     864 
   Size:        214      42      99     509

  Fragments arranged by size: 

                 509     214      99      42
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     816     850     864 
   Size:        816      34      14

  Fragments arranged by size: 

                 816      34      14
 
BclI T'GATC_A

Cuts at:      0     800     864 
   Size:        800      64
 
BglI GCCn_nnn'nGGC

Cuts at:      0     184     864 
   Size:        184     680
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     514     526     537     634     864 
   Size:        478      36      12      11      97     230

  Fragments arranged by size: 

                 478     230      97      36      12      11
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      71     864 
   Size:         71     793
 
BsaBI GATnn'nnATC

Cuts at:      0     805     864 
   Size:        805      59
 
BsaWI w'CCGG_w

Cuts at:      0     344     864 
   Size:        344     520
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     310     340     376     406     864 
   Size:        310      30      36      30     458

  Fragments arranged by size: 

                 458     310      36      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     127     169     232     315     502     864 
   Size:        127      42      63      83     187     362

  Fragments arranged by size: 

                 362     187     127      83      63      42
 
BsiHKAI G_wGCw'C

Cuts at:      0      23     864 
   Size:         23     841
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     580     864 
   Size:        580     284
 
Bsp1286I G_dGCh'C

Cuts at:      0      23     864 
   Size:         23     841
 
BspEI T'CCGG_A

Cuts at:      0     344     864 
   Size:        344     520
 
BspHI T'CATG_A

Cuts at:      0     797     864 
   Size:        797      67
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      96     864 
   Size:         96     768
 
BsrBI CCG'CTC

Cuts at:      0     323     864 
   Size:        323     541
 
BsrFI r'CCGG_y

Cuts at:      0     466     864 
   Size:        466     398
 
BssHII G'CGCG_C

Cuts at:      0     781     864 
   Size:        781      83
 
BssSI C'ACGA_G

Cuts at:      0     664     864 
   Size:        664     200
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     666     864 
   Size:        666     198
 
BstYI r'GATC_y

Cuts at:      0     341     864 
   Size:        341     523
 
Bsu36I CC'TnA_GG

Cuts at:      0     337     864 
   Size:        337     527
 
BtgI C'CryG_G

Cuts at:      0      68     864 
   Size:         68     796
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      56     819     864 
   Size:         56     763      45

  Fragments arranged by size: 

                 763      56      45
 
EaeI y'GGCC_r

Cuts at:      0      29     656     864 
   Size:         29     627     208

  Fragments arranged by size: 

                 627     208      29
 
EcoICRI GAG'CTC

Cuts at:      0      21     864 
   Size:         21     843
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     478     514     526     537     634     864 
   Size:        478      36      12      11      97     230

  Fragments arranged by size: 

                 478     230      97      36      12      11
 
HaeII r_GCGC'y

Cuts at:      0     432     864 
   Size:        432     432
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     376     408     864 
   Size:        376      32     456

  Fragments arranged by size: 

                 456     376      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     263     374     440     864 
   Size:        263     111      66     424

  Fragments arranged by size: 

                 424     263     111      66
 
MscI TGG'CCA

Cuts at:      0     658     864 
   Size:        658     206
 
MslI CAynn'nnrTG

Cuts at:      0     646     652     658     664     864 
   Size:        646       6       6       6     200

  Fragments arranged by size: 

                 646     200       6       6       6
 
MspA1I CmG'CkG

Cuts at:      0     331     456     864 
   Size:        331     125     408

  Fragments arranged by size: 

                 408     331     125
 
NspI r_CATG'y

Cuts at:      0     504     645     864 
   Size:        504     141     219

  Fragments arranged by size: 

                 504     219     141
 
PciI A'CATG_T

Cuts at:      0     641     864 
   Size:        641     223
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     310     342     864 
   Size:        310      32     522

  Fragments arranged by size: 

                 522     310      32
 
PvuII CAG'CTG

Cuts at:      0     456     864 
   Size:        456     408
 
SacI G_AGCT'C

Cuts at:      0      23     864 
   Size:         23     841
 
StuI AGG'CCT

Cuts at:      0     314     864 
   Size:        314     550
 
StyI C'CwwG_G

Cuts at:      0      32     864 
   Size:         32     832
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      23      89     864 
   Size:         23      66     775

  Fragments arranged by size: 

                 775      66      23
 
TatI w'GTAC_w

Cuts at:      0     398     701     864 
   Size:        398     303     163

  Fragments arranged by size: 

                 398     303     163
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     159     191     864 
   Size:        159      32     673

  Fragments arranged by size: 

                 673     159      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     378     489     864 
   Size:        378     111     375

  Fragments arranged by size: 

                 378     375     111
 

 Enzymes that do cut:

     AarI     AccI     AfeI   AflIII     AloI    AlwNI     AscI    BamHI
    BanII     BbsI     BcgI     BclI     BglI     BpmI     BsaI    BsaBI
    BsaWI    BsaXI    BseRI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI   BssHII    BssSI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     EaeI  EcoICRI  Eco57MI    HaeII    Hin4I     MmeI
     MscI     MslI   MspA1I     NspI     PciI     PpiI    PvuII     SacI
     StuI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI    AflII     AgeI     AhdI     AleI
     AlfI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII
     BaeI     BanI     BbeI    BbvCI    BciVI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI
    BsaHI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsrDI    BsrGI
   BstAPI    BstBI   BstEII  BstZ17I     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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