Restriction map for
pF1KB5319
(Circular) (Six-base) MAPSORT of: pF1KB5319.seq Check: 6587 from: 1 to: 4391
pF1KB5319 4391 bp
With 182 enzymes: SgfI *
June 20, 2008 10:25 ..
AccI GT'mk_AC
Cuts at: 815 1429 815
Size: 614 3777
Acc65I G'GTAC_C
Cuts at: 1407 1407
Size: 4391
AclI AA'CG_TT
Cuts at: 4231 4231
Size: 4391
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 382 2216 2648 3001 3663 250
Size: 132 1834 432 353 662 978
Fragments arranged by size:
1834 978 662 432 353 132
AflIII A'CryG_T
Cuts at: 1743 3543 3824 1743
Size: 1800 281 2310
Fragments arranged by size:
2310 1800 281
AleI CACnn'nnGTG
Cuts at: 868 868
Size: 4391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1069 1069
Size: 4391
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 974 1006 974
Size: 32 4359
AlwNI CAG_nnn'CTG
Cuts at: 1329 1696 3134 1329
Size: 367 1438 2586
Fragments arranged by size:
2586 1438 367
ApaLI G'TGCA_C
Cuts at: 3229 3229
Size: 4391
ApoI r'AATT_y
Cuts at: 948 1133 1150 1395 3744 4000 948
Size: 185 17 245 2349 256 1339
Fragments arranged by size:
2349 1339 256 245 185 17
AseI AT'TA_AT
Cuts at: 20 20
Size: 4391
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
AvaI C'yCGr_G
Cuts at: 1411 1411
Size: 4391
BamHI G'GATC_C
Cuts at: 1416 1416
Size: 4391
BanI G'GyrC_C
Cuts at: 244 1407 1821 2069 2104 244
Size: 1163 414 248 35 2531
Fragments arranged by size:
2531 1163 414 248 35
BanII G_rGCy'C
Cuts at: 827 1405 2435 827
Size: 578 1030 2783
Fragments arranged by size:
2783 1030 578
BbeI G_GCGC'C
Cuts at: 1825 2073 1825
Size: 248 4143
BbvCI CC'TCA_GC
Cuts at: 551 551
Size: 4391
BciVI GTATCCnnnnn_n'
Cuts at: 2289 3345 4391 2289
Size: 1056 1046 2289
Fragments arranged by size:
2289 1056 1046
BclI T'GATC_A
Cuts at: 1911 1911
Size: 4391
BglII A'GATC_T
Cuts at: 1906 3943 1906
Size: 2037 2354
BlpI GC'TnA_GC
Cuts at: 1503 1503
Size: 4391
Bme1580I G_kGCm'C
Cuts at: 2016 2109 3233 2016
Size: 93 1124 3174
Fragments arranged by size:
3174 1124 93
BmrI ACTGGGnnnn_n'
Cuts at: 1603 1684 2019 4163 1603
Size: 81 335 2144 1831
Fragments arranged by size:
2144 1831 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 4391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1306 1306
Size: 4391
Bpu10I CC'TnA_GC
Cuts at: 551 551
Size: 4391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 1567 2446 3160 3458 291
Size: 1276 879 714 298 1224
Fragments arranged by size:
1276 1224 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4095 4095
Size: 4391
BsaAI yAC'GTr
Cuts at: 1228 1744 1228
Size: 516 3875
BsaBI GATnn'nnATC
Cuts at: 6 1905 3942 6
Size: 1899 2037 455
Fragments arranged by size:
2037 1899 455
BsaHI Gr'CG_yC
Cuts at: 1822 2070 2772 4268 1822
Size: 248 702 1496 1945
Fragments arranged by size:
1945 1496 702 248
BsaWI w'CCGG_w
Cuts at: 344 944 1580 2101 2779 3190 3337 3611 344
Size: 600 636 521 678 411 147 274 1124
Fragments arranged by size:
1124 678 636 600 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1001 1031 1001
Size: 30 4361
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 849 849
Size: 4391
BseYI C'CCAG_C
Cuts at: 1247 1816 2574 3239 3647 1247
Size: 569 758 665 408 1991
Fragments arranged by size:
1991 758 665 569 408
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1294 1294
Size: 4391
BsiEI CG_ry'CG
Cuts at: 104 1933 1979 3209 104
Size: 1829 46 1230 1286
Fragments arranged by size:
1829 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 808 827 1405 2183 2373 3233 808
Size: 19 578 778 190 860 1966
Fragments arranged by size:
1966 860 778 578 190 19
BsmI GAATG_Cn'
Cuts at: 433 1033 433
Size: 600 3791
Bsp1286I G_dGCh'C
Cuts at: 808 827 1405 2016 2109 2183 2373 2435 3233
Size: 19 578 611 93 74 190 62 798
Cuts at: 3233 808
Size: 1966
Fragments arranged by size:
1966 798 611 578 190 93 74 62
19
BspHI T'CATG_A
Cuts at: 2823 2823
Size: 4391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1441 1655 1957 2338 1441
Size: 214 302 381 3494
Fragments arranged by size:
3494 381 302 214
BsrBI CCG'CTC
Cuts at: 50 314 2683 2737 4221 4386 50
Size: 264 2369 54 1484 165 55
Fragments arranged by size:
2369 1484 264 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2303 2303
Size: 4391
BsrFI r'CCGG_y
Cuts at: 2389 2570 2389
Size: 181 4210
BssSI C'ACGA_G
Cuts at: 2662 3370 2662
Size: 708 3683
BstAPI GCAn_nnn'nTGC
Cuts at: 1289 1768 1289
Size: 479 3912
BstBI TT'CG_AA
Cuts at: 2752 2752
Size: 4391
BstXI CCAn_nnnn'nTGG
Cuts at: 800 800
Size: 4391
BstYI r'GATC_y
Cuts at: 1 1109 1416 1883 1906 2241 2487 2793 2805
Size: 1108 307 467 23 335 246 306 12
Cuts at: 2805 2891 3943 1
Size: 86 1052 449
Fragments arranged by size:
1108 1052 467 449 335 307 306 246
86 23 12
BtgI C'CryG_G
Cuts at: 248 1182 248
Size: 934 3457
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 191 2341 2522 2685 191
Size: 2150 181 163 1897
Fragments arranged by size:
2150 1897 181 163
BtsI GCAGTG_nn'
Cuts at: 1634 1634
Size: 4391
DraI TTT'AAA
Cuts at: 1390 1390
Size: 4391
DrdI GACnn_nn'nnGTC
Cuts at: 2097 3441 2097
Size: 1344 3047
EaeI y'GGCC_r
Cuts at: 1976 2150 2541 2568 3913 1976
Size: 174 391 27 1345 2454
Fragments arranged by size:
2454 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1976 1976
Size: 4391
EarI CTCTTCn'nnn_
Cuts at: 145 2414 2624 145
Size: 2269 210 1912
Fragments arranged by size:
2269 1912 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2599 3341 3487 4201 2599
Size: 742 146 714 2789
Fragments arranged by size:
2789 742 714 146
EcoICRI GAG'CTC
Cuts at: 825 1403 825
Size: 578 3813
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 382 1306 2216 2648 3001 3663 250
Size: 132 924 910 432 353 662 978
Fragments arranged by size:
978 924 910 662 432 353 132
EcoO109I rG'GnC_Cy
Cuts at: 1530 1530
Size: 4391
EcoRI G'AATT_C
Cuts at: 948 1395 948
Size: 447 3944
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2410 2410
Size: 4391
FspI TGC'GCA
Cuts at: 2172 2172
Size: 4391
HaeII r_GCGC'y
Cuts at: 387 1825 2073 3303 4074 387
Size: 1438 248 1230 771 704
Fragments arranged by size:
1438 1230 771 704 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2233 2263 2265 2295 2233
Size: 30 2 30 4329
Fragments arranged by size:
4329 30 30 2
HincII GTy'rAC
Cuts at: 942 1430 942
Size: 488 3903
HpaI GTT'AAC
Cuts at: 942 942
Size: 4391
KasI G'GCGC_C
Cuts at: 1821 2069 1821
Size: 248 4143
KpnI G_GTAC'C
Cuts at: 1411 1411
Size: 4391
MluI A'CGCG_T
Cuts at: 3824 3824
Size: 4391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 923 987 1042 3150 3334 923
Size: 64 55 2108 184 1980
Fragments arranged by size:
2108 1980 184 64 55
MscI TGG'CCA
Cuts at: 2152 2152
Size: 4391
MslI CAynn'nnrTG
Cuts at: 112 426 868 1051 2380 2507 112
Size: 314 442 183 1329 127 1996
Fragments arranged by size:
1996 1329 442 314 183 127
MspA1I CmG'CkG
Cuts at: 145 1502 1816 2176 2960 3205 145
Size: 1357 314 360 784 245 1331
Fragments arranged by size:
1357 1331 784 360 314 245
NaeI GCC'GGC
Cuts at: 2572 2572
Size: 4391
NarI GG'CG_CC
Cuts at: 1822 2070 1822
Size: 248 4143
NcoI C'CATG_G
Cuts at: 248 1182 248
Size: 934 3457
NgoMIV G'CCGG_C
Cuts at: 2570 2570
Size: 4391
NspI r_CATG'y
Cuts at: 606 1444 1944 3547 606
Size: 838 500 1603 1450
Fragments arranged by size:
1603 1450 838 500
PasI CC'CwG_GG
Cuts at: 280 280
Size: 4391
PciI A'CATG_T
Cuts at: 3543 3543
Size: 4391
PflMI CCAn_nnn'nTGG
Cuts at: 1329 1329
Size: 4391
PmeI GTTT'AAAC
Cuts at: 1390 1390
Size: 4391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 4359
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1057 1089 1057
Size: 32 4359
PstI C_TGCA'G
Cuts at: 1438 2123 1438
Size: 685 3706
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4391
PvuII CAG'CTG
Cuts at: 145 1816 2176 145
Size: 1671 360 2360
Fragments arranged by size:
2360 1671 360
RsrII CG'GwC_CG
Cuts at: 2586 2586
Size: 4391
SacI G_AGCT'C
Cuts at: 827 1405 827
Size: 578 3813
SalI G'TCGA_C
Cuts at: 1428 1428
Size: 4391
SapI GCTCTTCn'nnn_
Cuts at: 2414 2624 2414
Size: 210 4181
SbfI CC_TGCA'GG
Cuts at: 1438 1438
Size: 4391
ScaI AGT'ACT
Cuts at: 3908 3908
Size: 4391
SfcI C'TryA_G
Cuts at: 33 1434 2119 3087 3278 33
Size: 1401 685 968 191 1146
Fragments arranged by size:
1401 1146 968 685 191
SfoI GGC'GCC
Cuts at: 1823 2071 1823
Size: 248 4143
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4391
SmaI CCC'GGG
Cuts at: 1413 1413
Size: 4391
SmlI C'TyrA_G
Cuts at: 270 1546 2461 3175 3437 270
Size: 1276 915 714 262 1224
Fragments arranged by size:
1276 1224 915 714 262
SnaBI TAC'GTA
Cuts at: 1228 1228
Size: 4391
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4391
SphI G_CATG'C
Cuts at: 1444 1944 1444
Size: 500 3891
StuI AGG'CCT
Cuts at: 268 268
Size: 4391
StyI C'CwwG_G
Cuts at: 248 921 1182 1525 1879 248
Size: 673 261 343 354 2760
Fragments arranged by size:
2760 673 354 343 261
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 152 829 1109 1974 2107 2566 2775 152
Size: 677 280 865 133 459 209 1768
Fragments arranged by size:
1768 865 677 459 280 209 133
TatI w'GTAC_w
Cuts at: 507 1219 3906 507
Size: 712 2687 992
Fragments arranged by size:
2687 992 712
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1001 1033 3908 3940 1001
Size: 32 2875 32 1452
Fragments arranged by size:
2875 1452 32 32
Tth111I GACn'n_nGTC
Cuts at: 2188 2188
Size: 4391
XbaI T'CTAG_A
Cuts at: 1422 1422
Size: 4391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 255 870 1886 255
Size: 615 1016 2760
Fragments arranged by size:
2760 1016 615
XmaI C'CCGG_G
Cuts at: 1411 1411
Size: 4391
XmnI GAAnn'nnTTC
Cuts at: 913 913
Size: 4391
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PsrI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BglI BmgBI BmtI
BsiWI BsmBI BspEI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI CspCI DraIII EcoNI EcoRV FseI FspAI HindIII
MfeI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI SacII SanDI
SexAI SfiI SgrAI SrfI SspI SwaI XhoI ZraI