Restriction map for pF1KB5309
##################################
# NO CUT ENZYMES (  121 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
ApoI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BalI        
BamHI       BanII       BbvCI       BciVI       BclI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrGI       BssSI       Bst1107I    BstAPI      
BstBI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DrdI        Eam1105I    
Eco47III    Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NdeI        NheI        NotI        NsiI        
NspV        PacI        PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SalI        
SbfI        SfcI        SfiI        SfuI        SgrAI       
SnaBI       Sse8387I    SspBI       SspI        SwaI        
TfiI        Tru9I       Tsp509I     Tth111I     XbaI        
XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AscI          884  GGCGCGCC             GG^CGCGCC
BbeI         1213  GGCGCC               GGCGC^C
BbrPI         549  CACGTG               CAC^GTG
BbsI          243  GAAGAC               GAAGAC(2/6)
BfaI           68  CTAG                 C^TAG
BglI          115  GCCGGCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         243  GAAGAC               GAAGAC(2/6)
BsaAI         549  CACGTG               [CT]AC^GT[GA]
BsrDI         335  GCAATG               GCAATG(2/0)
BsrI         1194  CCAGT                ACTGG(1/-1)
BstEII       1227  GGTCACC              G^GT[ACGT]ACC
BstPI        1227  GGTCACC              G^GT[ACGT]ACC
BstYI        1190  AGATCC               [GA]^GATC[CT]
DdeI           38  CTAAG                C^T[ACGT]AG
EcoNI         372  CCTGGTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1227  GGTCACC              G^GT[ACGT]ACC
EcoT14I        32  CCATGG               C^C[AT][AT]GG
HaeII        1213  GGCGCC               [GA]GCGC^[CT]
HphI         1229  TCACC                GGTGA(8/7)
KasI         1213  GGCGCC               G^GCGCC
MaeI           68  CTAG                 C^TAG
MaeIII       1228  GTCAC                ^GT[ACGT]AC
MflI         1190  AGATCC               [GA]^GATC[CT]
MslI          477  CATCACGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI         1213  GGCGCC               GG^CGCC
NcoI           32  CCATGG               C^CATGG
NruI         1251  TCGCGA               TCG^CGA
PaeR7I        169  CTCGAG               C^TCGAG
PmaCI         549  CACGTG               CAC^GTG
PmlI          549  CACGTG               CAC^GTG
PpuMI         788  AGGACCT              [GA]G^G[AT]CC[CT]
ScaI          758  AGTACT               AGT^ACT
SexAI         740  ACCTGGT              A^CC[AT]GGT
SfoI         1213  GGCGCC               GGC^GCC
SmaI          391  CCCGGG               CCC^GGG
SpeI           67  ACTAGT               A^CTAGT
StuI          959  AGGCCT               AGG^CCT
StyI           32  CCATGG               C^C[AT][AT]GG
Tsp45I       1228  GTCAC                ^GT[GC]AC
TspRI         182  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI         1109  CCAAGTCCATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          169  CTCGAG               C^TCGAG
XhoII        1190  AGATCC               [GA]^GATC[CT]
XmaCI         391  CCCGGG               C^CCGGG
XmaI          391  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcyI         1057  GACGCC               G[GA]^CG[CT]C
             1213  GGCGCC              
BcgI          335  GCAATGACTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              378  GCAGGACTATCG        
BpmI          718  CTGGAG               CTGGAG(16/14)
              808  CTGGAG              
BsaHI        1057  GACGCC               G[GA]^CG[CT]C
             1213  GGCGCC              
BsiEI         330  CGGCCG               CG[GA][CT]^CG
              563  CGGCCG              
BssHII         56  GCGCGC               G^CGCGC
              885  GCGCGC              
DraII         394  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              788  AGGACCT             
DraIII        739  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1048  CACGCCGTG           
EagI          330  CGGCCG               C^GGCCG
              563  CGGCCG              
EclXI         330  CGGCCG               C^GGCCG
              563  CGGCCG              
Eco52I        330  CGGCCG               C^GGCCG
              563  CGGCCG              
EcoO109I      394  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              788  AGGACCT             
Hin1I        1057  GACGCC               G[GA]^CG[CT]C
             1213  GGCGCC              
KspI          220  CCGCGG               CCGC^GG
              859  CCGCGG              
MaeII         550  ACGT                 A^CGT
              815  ACGT                
SacII         220  CCGCGG               CCGC^GG
              859  CCGCGG              
SfaNI        1034  GCATC                GCATC(5/9)
             1142  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BanI        
BbvI        BcnI        BmyI        BsaJI       BsgI        
BsiHKAI     BsiYI       BslI        BsoBI       Bsp1286I    
BspMI       BsrFI       BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DpnII       DsaI        EaeI        EarI        EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
ItaI        Ksp632I     MboI        MboII       MnlI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       NspI        PflMI       PleI        RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SmlI        
SphI        TaqI        TseI        TthHB8I     Van91I      
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