Restriction map for pF1KB5309
 (Linear) (Six-base) MAPSORT of: KIBB5309.seq  Check: 29  from: 1  to: 1269



KIBB5309.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     171     690    1269 
   Size:        171     519     579

  Fragments arranged by size: 

                 579     519     171
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     805    1269 
   Size:        805     464
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     168     286     391     439    1269 
   Size:        168     118     105      48     830

  Fragments arranged by size: 

                 830     168     118     105      48
 
AlwNI CAG_nnn'CTG

Cuts at:      0     944    1101    1200    1269 
   Size:        944     157      99      69

  Fragments arranged by size: 

                 944     157      99      69
 
AscI GG'CGCG_CC

Cuts at:      0     885    1269 
   Size:        885     384
 
AvaI C'yCGr_G

Cuts at:      0     169     391     867    1186    1269 
   Size:        169     222     476     319      83

  Fragments arranged by size: 

                 476     319     222     169      83
 
BanI G'GyrC_C

Cuts at:      0     456     540    1105    1213    1269 
   Size:        456      84     565     108      56

  Fragments arranged by size: 

                 565     456     108      84      56
 
BbeI G_GCGC'C

Cuts at:      0    1217    1269 
   Size:       1217      52
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     250    1269 
   Size:        250    1019
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     324     358     367     401    1269 
   Size:        324      34       9      34     868

  Fragments arranged by size: 

                 868     324      34      34       9
 
BglI GCCn_nnn'nGGC

Cuts at:      0     121    1269 
   Size:        121    1148
 
Bme1580I G_kGCm'C

Cuts at:      0     104     545    1108    1269 
   Size:        104     441     563     161

  Fragments arranged by size: 

                 563     441     161     104
 
BmgBI CAC'GTC

Cuts at:      0     816    1269 
   Size:        816     453
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     739     829    1269 
   Size:        739      90     440

  Fragments arranged by size: 

                 739     440      90
 
Bpu10I CC'TnA_GC

Cuts at:      0      38    1269 
   Size:         38    1231
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295    1117    1269 
   Size:        295     822     152

  Fragments arranged by size: 

                 822     295     152
 
BsaAI yAC'GTr

Cuts at:      0     551    1269 
   Size:        551     718
 
BsaHI Gr'CG_yC

Cuts at:      0    1058    1214    1269 
   Size:       1058     156      55

  Fragments arranged by size: 

                1058     156      55
 
BseYI C'CCAG_C

Cuts at:      0      51     688     800    1101    1269 
   Size:         51     637     112     301     168

  Fragments arranged by size: 

                 637     301     168     112      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     397     724    1269 
   Size:        149     248     327     545

  Fragments arranged by size: 

                 545     327     248     149
 
BsiEI CG_ry'CG

Cuts at:      0     333     566    1269 
   Size:        333     233     703

  Fragments arranged by size: 

                 703     333     233
 
BsiHKAI G_wGCw'C

Cuts at:      0     488     713     749     821    1269 
   Size:        488     225      36      72     448

  Fragments arranged by size: 

                 488     448     225      72      36
 
Bsp1286I G_dGCh'C

Cuts at:      0     104     488     545     713     749     821    1108    1269 
   Size:        104     384      57     168      36      72     287     161

  Fragments arranged by size: 

                 384     287     168     161     104      72      57      36
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     171     656     690     800    1269 
   Size:        171     485      34     110     469

  Fragments arranged by size: 

                 485     469     171     110      34
 
BsrDI GCAATG_nn'

Cuts at:      0     342    1269 
   Size:        342     927
 
BsrFI r'CCGG_y

Cuts at:      0     115     305     534     565    1169    1269 
   Size:        115     190     229      31     604     100

  Fragments arranged by size: 

                 604     229     190     115     100      31
 
BssHII G'CGCG_C

Cuts at:      0      56     885    1269 
   Size:         56     829     384

  Fragments arranged by size: 

                 829     384      56
 
BstEII G'GTnAC_C

Cuts at:      0    1227    1269 
   Size:       1227      42
 
BstYI r'GATC_y

Cuts at:      0    1190    1269 
   Size:       1190      79
 
BtgI C'CryG_G

Cuts at:      0      32     220     859    1052    1269 
   Size:         32     188     639     193     217

  Fragments arranged by size: 

                 639     217     193     188      32
 
BtsI GCAGTG_nn'

Cuts at:      0     188    1269 
   Size:        188    1081
 
DraIII CAC_nnn'GTG

Cuts at:      0     744    1053    1269 
   Size:        744     309     216

  Fragments arranged by size: 

                 744     309     216
 
EaeI y'GGCC_r

Cuts at:      0     257     294     330     563     992    1269 
   Size:        257      37      36     233     429     277

  Fragments arranged by size: 

                 429     277     257     233      37      36
 
EagI C'GGCC_G

Cuts at:      0     330     563    1269 
   Size:        330     233     706

  Fragments arranged by size: 

                 706     330     233
 
EarI CTCTTCn'nnn_

Cuts at:      0      67     658    1132    1269 
   Size:         67     591     474     137

  Fragments arranged by size: 

                 591     474     137      67
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     739     805     829    1269 
   Size:        739      66      24     440

  Fragments arranged by size: 

                 739     440      66      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     376    1269 
   Size:        376     893
 
EcoO109I rG'GnC_Cy

Cuts at:      0     395     789    1269 
   Size:        395     394     480

  Fragments arranged by size: 

                 480     395     394
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     336    1269 
   Size:        336     933
 
HaeII r_GCGC'y

Cuts at:      0    1217    1269 
   Size:       1217      52
 
KasI G'GCGC_C

Cuts at:      0    1213    1269 
   Size:       1213      56
 
MslI CAynn'nnrTG

Cuts at:      0     481    1269 
   Size:        481     788
 
MspA1I CmG'CkG

Cuts at:      0     108     222     861    1101    1152    1269 
   Size:        108     114     639     240      51     117

  Fragments arranged by size: 

                 639     240     117     114     108      51
 
NaeI GCC'GGC

Cuts at:      0     117     567    1171    1269 
   Size:        117     450     604      98

  Fragments arranged by size: 

                 604     450     117      98
 
NarI GG'CG_CC

Cuts at:      0    1214    1269 
   Size:       1214      55
 
NcoI C'CATG_G

Cuts at:      0      32    1269 
   Size:         32    1237
 
NgoMIV G'CCGG_C

Cuts at:      0     115     565    1169    1269 
   Size:        115     450     604     100

  Fragments arranged by size: 

                 604     450     115     100
 
NruI TCG'CGA

Cuts at:      0    1253    1269 
   Size:       1253      16
 
NspI r_CATG'y

Cuts at:      0     115     426     906    1023    1179    1269 
   Size:        115     311     480     117     156      90

  Fragments arranged by size: 

                 480     311     156     117     115      90
 
PasI CC'CwG_GG

Cuts at:      0      23    1269 
   Size:         23    1246
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     896     944    1053    1223    1238    1269 
   Size:        896      48     109     170      15      31

  Fragments arranged by size: 

                 896     170     109      48      31      15
 
PfoI T'CCnGG_A

Cuts at:      0     716    1070    1269 
   Size:        716     354     199

  Fragments arranged by size: 

                 716     354     199
 
PmlI CAC'GTG

Cuts at:      0     551    1269 
   Size:        551     718
 
PpuMI rG'GwC_Cy

Cuts at:      0     789    1269 
   Size:        789     480
 
PspXI vC'TCGA_Gb

Cuts at:      0     169    1269 
   Size:        169    1100
 
SacII CC_GC'GG

Cuts at:      0     223     862    1269 
   Size:        223     639     407

  Fragments arranged by size: 

                 639     407     223
 
SapI GCTCTTCn'nnn_

Cuts at:      0      67     658    1132    1269 
   Size:         67     591     474     137

  Fragments arranged by size: 

                 591     474     137      67
 
ScaI AGT'ACT

Cuts at:      0     760    1269 
   Size:        760     509
 
SexAI A'CCwGG_T

Cuts at:      0     740    1269 
   Size:        740     529
 
SfoI GGC'GCC

Cuts at:      0    1215    1269 
   Size:       1215      54
 
SmaI CCC'GGG

Cuts at:      0     393    1269 
   Size:        393     876
 
SmlI C'TyrA_G

Cuts at:      0     169     310    1132    1269 
   Size:        169     141     822     137

  Fragments arranged by size: 

                 822     169     141     137
 
SpeI A'CTAG_T

Cuts at:      0      67    1269 
   Size:         67    1202
 
SphI G_CATG'C

Cuts at:      0     115    1023    1179    1269 
   Size:        115     908     156      90

  Fragments arranged by size: 

                 908     156     115      90
 
StuI AGG'CCT

Cuts at:      0     961    1269 
   Size:        961     308
 
StyI C'CwwG_G

Cuts at:      0      32    1269 
   Size:         32    1237
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     595    1269 
   Size:        595     674
 
TatI w'GTAC_w

Cuts at:      0      86     758    1269 
   Size:         86     672     511

  Fragments arranged by size: 

                 672     511      86
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     781     813    1269 
   Size:        781      32     456

  Fragments arranged by size: 

                 781     456      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1116    1269 
   Size:       1116     153
 
XhoI C'TCGA_G

Cuts at:      0     169    1269 
   Size:        169    1100
 
XmaI C'CCGG_G

Cuts at:      0     391    1269 
   Size:        391     878
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI     AscI     AvaI     BanI     BbeI
     BbsI     BcgI     BglI Bme1580I    BmgBI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaHI    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspMI
    BsrDI    BsrFI   BssHII   BstEII    BstYI     BtgI     BtsI   DraIII
     EaeI     EagI     EarI  Eco57MI    EcoNI EcoO109I     FalI    HaeII
     KasI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NruI
     NspI     PasI    PflMI     PfoI     PmlI    PpuMI    PspXI    SacII
     SapI     ScaI    SexAI     SfoI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII    BbvCI    BciVI     BclI    BfrBI
    BglII     BlpI     BmrI     BmtI     BplI     BsaI    BsaBI    BsaWI
    BsaXI    BseRI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrGI    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI
     ClaI    CspCI     DraI     DrdI     EciI  EcoICRI    EcoRI    EcoRV
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     NdeI     NheI     NotI     NsiI
     PacI     PciI     PmeI     PpiI    PshAI     PsiI   PspOMI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI     SbfI
     SfcI     SfiI     SgfI    SgrAI    SnaBI     SrfI     SspI     SwaI
  Tth111I     XbaI     XmnI     ZraI
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