Restriction map for pF1KB5228
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        AlwI        
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BsaAI       
BsaBI       BsaI        BseAI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrGI       
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstYI       BstZ17I     ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KasI        KpnI        MaeII       MamI        MboI        
MfeI        MflI        MroI        MseI        MunI        
NarI        NcoI        NdeI        NdeII       NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacI        SalI        SapI        
Sau3AI      SexAI       SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SspBI       
SspI        SwaI        TfiI        Tru9I       Tth111I     
XbaI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AcyI            9  GGCGTC               G[GA]^CG[CT]C
AflIII       1083  ACGCGT               A^C[GA][CT]GT
Alw44I        649  GTGCAC               G^TGCAC
AocI          762  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       717  AGCGCT               AGC^GCT
ApaLI         649  GTGCAC               G^TGCAC
AspHI         649  GTGCAC               G[AT]GC[AT]^C
BalI          932  TGGCCA               TGG^CCA
BbsI         1049  GAAGAC               GAAGAC(2/6)
BbvCI         333  CCTCAGC              CCTCAGC(-5/-2)
BfaI          901  CTAG                 C^TAG
BlpI         1171  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     1171  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1049  GAAGAC               GAAGAC(2/6)
BsaHI           9  GGCGTC               G[GA]^CG[CT]C
BsaWI         353  TCCGGT               [AT]^CCGG[AT]
BsiEI         612  CGACCG               CG[GA][CT]^CG
BsiHKAI       649  GTGCAC               G[AT]GC[AT]^C
BsmAI         617  GTCTC                GTCTC(1/5)
BsmBI         616  CGTCTC               CGTCTC(1/5)
BspHI         683  TCATGA               T^CATGA
BsrBI        1180  CCGCTC               CCGCTC(-3/-3)
BsrDI        1067  GCAATG               GCAATG(2/0)
BssHII        585  GCGCGC               G^CGCGC
Bsu36I        762  CCTGAGG              CC^T[ACGT]AGG
CelII        1171  GCTGAGC              GC^T[ACGT]AGC
Eco47III      717  AGCGCT               AGC^GCT
Eco81I        762  CCTGAGG              CC^T[ACGT]AGG
Hin1I           9  GGCGTC               G[GA]^CG[CT]C
MaeI          901  CTAG                 C^TAG
MluI         1083  ACGCGT               A^CGCGT
MluNI         932  TGGCCA               TGG^CCA
MscI          932  TGGCCA               TGG^CCA
NaeI           26  GCCGGC               GCC^GGC
NgoMIV         26  GCCGGC               G^CCGGC
RcaI          683  TCATGA               T^CATGA
ScaI         1035  AGTACT               AGT^ACT
SmaI          438  CCCGGG               CCC^GGG
SphI          657  GCATGC               GCATG^C
Tsp509I       285  AATT                 ^AATT
XmaCI         438  CCCGGG               C^CCGGG
XmaI          438  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

EarI          547  CTCTTC               CTCTTC(1/4)
                3  GAAGAG              
EcoT14I       110  CCAAGG               C^C[AT][AT]GG
              672  CCTTGG              
HaeII         717  AGCGCT               [GA]GCGC^[CT]
             1386  GGCGCT              
HgaI         1082  GACGC                GACGC(5/10)
               10  GCGTC               
Ksp632I       547  CTCTTC               CTCTTC(1/4)
                3  GAAGAG              
KspI          450  CCGCGG               CCGC^GG
              834  CCGCGG              
NspI          210  GCATGT               [GA]CATG^[CT]
              657  GCATGC              
PflMI         251  CCATCCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              378  CCACGGCCTGG         
PvuII         218  CAGCTG               CAG^CTG
              325  CAGCTG              
SacII         450  CCGCGG               CCGC^GG
              834  CCGCGG              
StuI           56  AGGCCT               AGG^CCT
             1028  AGGCCT              
StyI          110  CCAAGG               C^C[AT][AT]GG
              672  CCTTGG              
TaqI          268  TCGA                 T^CGA
              611  TCGA                
TthHB8I       268  TCGA                 T^CGA
              611  TCGA                
Van91I        251  CCATCCTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              378  CCACGGCCTGG         
XcmI          725  CCACTGAGGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              808  CCACTCTGGGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
ApaI        AvaI        AvaII       BanI        BanII       
BbvI        BcnI        BglI        BmyI        BpmI        
BsaJI       BseRI       BsgI        BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BspMI       BsrFI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DraII       DsaI        EaeI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeIII      MboII       MnlI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NlaIII      NlaIV       PleI        
PstI        RsaI        Sau96I      SbfI        ScrFI       
SfaNI       SfcI        Sse8387I    TseI        Tsp45I      
TspRI       
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