Restriction map for pF1KB5228
 (Linear) (Six-base) MAPSORT of: KIBB5228.seq  Check: 3489  from: 1  to: 1419



KIBB5228.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      54    1369    1419 
   Size:         54    1315      50

  Fragments arranged by size: 

                1315      54      50
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1096    1419 
   Size:       1096     323
 
AfeI AGC'GCT

Cuts at:      0     719    1419 
   Size:        719     700
 
AflIII A'CryG_T

Cuts at:      0    1083    1419 
   Size:       1083     336
 
AleI CACnn'nnGTG

Cuts at:      0    1189    1419 
   Size:       1189     230
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      93     205     674    1419 
   Size:         93     112     469     745

  Fragments arranged by size: 

                 745     469     112      93
 
AlwNI CAG_nnn'CTG

Cuts at:      0     141     432     504     762    1283    1419 
   Size:        141     291      72     258     521     136

  Fragments arranged by size: 

                 521     291     258     141     136      72
 
ApaI G_GGCC'C

Cuts at:      0     439    1237    1415    1419 
   Size:        439     798     178       4

  Fragments arranged by size: 

                 798     439     178       4
 
ApaLI G'TGCA_C

Cuts at:      0     649    1419 
   Size:        649     770
 
AvaI C'yCGr_G

Cuts at:      0     438     580    1198    1230    1419 
   Size:        438     142     618      32     189

  Fragments arranged by size: 

                 618     438     189     142      32
 
BanI G'GyrC_C

Cuts at:      0      85     852    1342    1419 
   Size:         85     767     490      77

  Fragments arranged by size: 

                 767     490      85      77
 
BanII G_rGCy'C

Cuts at:      0     110     439    1178    1207    1237    1415    1419 
   Size:        110     329     739      29      30     178       4

  Fragments arranged by size: 

                 739     329     178     110      30      29       4
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1056    1419 
   Size:       1056     363
 
BbvCI CC'TCA_GC

Cuts at:      0     334    1419 
   Size:        334    1085
 
BglI GCCn_nnn'nGGC

Cuts at:      0     881     990    1109    1292    1301    1419 
   Size:        881     109     119     183       9     118

  Fragments arranged by size: 

                 881     183     119     118     109       9
 
BlpI GC'TnA_GC

Cuts at:      0    1172    1419 
   Size:       1172     247
 
Bme1580I G_kGCm'C

Cuts at:      0     155     439     581     653     753     804     857    1237 
   Size:        155     284     142      72     100      51      53     380

Cuts at:   1237    1347    1415    1419 
   Size:        110      68       4

  Fragments arranged by size: 

                 380     284     155     142     110     100      72      68
                  53      51       4
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     340     555    1060    1419 
   Size:         38     302     215     505     359

  Fragments arranged by size: 

                 505     359     302     215      38
 
Bpu10I CC'TnA_GC

Cuts at:      0     334    1338    1419 
   Size:        334    1004      81

  Fragments arranged by size: 

                1004     334      81
 
BsaHI Gr'CG_yC

Cuts at:      0      10    1419 
   Size:         10    1409
 
BsaWI w'CCGG_w

Cuts at:      0     353    1419 
   Size:        353    1066
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     553     583    1419 
   Size:        553      30     836

  Fragments arranged by size: 

                 836     553      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     831     834     994    1419 
   Size:        831       3     160     425

  Fragments arranged by size: 

                 831     425     160       3
 
BseYI C'CCAG_C

Cuts at:      0     131     432     987    1351    1419 
   Size:        131     301     555     364      68

  Fragments arranged by size: 

                 555     364     301     131      68
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     247     388     538     586     610    1419 
   Size:        175      72     141     150      48      24     809

  Fragments arranged by size: 

                 809     175     150     141      72      48      24
 
BsiEI CG_ry'CG

Cuts at:      0     615    1419 
   Size:        615     804
 
BsiHKAI G_wGCw'C

Cuts at:      0     653    1419 
   Size:        653     766
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     622    1419 
   Size:        622     797
 
Bsp1286I G_dGCh'C

Cuts at:      0     110     155     439     581     653     753     804     857 
   Size:        110      45     284     142      72     100      51      53

Cuts at:    857    1178    1207    1237    1347    1415    1419 
   Size:        321      29      30     110      68       4

  Fragments arranged by size: 

                 321     284     142     110     110     100      72      68
                  53      51      45      30      29       4
 
BspHI T'CATG_A

Cuts at:      0     683    1419 
   Size:        683     736
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      54     488     923    1369    1419 
   Size:         54     434     435     446      50

  Fragments arranged by size: 

                 446     435     434      54      50
 
BsrBI CCG'CTC

Cuts at:      0    1182    1419 
   Size:       1182     237
 
BsrDI GCAATG_nn'

Cuts at:      0    1074    1419 
   Size:       1074     345
 
BsrFI r'CCGG_y

Cuts at:      0      26     937     961    1419 
   Size:         26     911      24     458

  Fragments arranged by size: 

                 911     458      26      24
 
BssHII G'CGCG_C

Cuts at:      0     585    1419 
   Size:        585     834
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     141     150     504    1069    1283    1419 
   Size:        141       9     354     565     214     136

  Fragments arranged by size: 

                 565     354     214     141     136       9
 
Bsu36I CC'TnA_GG

Cuts at:      0     763    1419 
   Size:        763     656
 
BtgI C'CryG_G

Cuts at:      0     155     378     450     594     834    1126    1419 
   Size:        155     223      72     144     240     292     293

  Fragments arranged by size: 

                 293     292     240     223     155     144      72
 
BtsI GCAGTG_nn'

Cuts at:      0     502    1184    1196    1281    1419 
   Size:        502     682      12      85     138

  Fragments arranged by size: 

                 682     502     138      85      12
 
EaeI y'GGCC_r

Cuts at:      0     675     882     932    1419 
   Size:        675     207      50     487

  Fragments arranged by size: 

                 675     487     207      50
 
EarI CTCTTCn'nnn_

Cuts at:      0     553    1419 
   Size:        553     866
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      38     340     555    1060    1096    1419 
   Size:         38     302     215     505      36     323

  Fragments arranged by size: 

                 505     323     302     215      38      36
 
EcoO109I rG'GnC_Cy

Cuts at:      0     138     732    1234    1412    1419 
   Size:        138     594     502     178       7

  Fragments arranged by size: 

                 594     502     178     138       7
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1073    1419 
   Size:       1073     346
 
HaeII r_GCGC'y

Cuts at:      0     721    1390    1419 
   Size:        721     669      29

  Fragments arranged by size: 

                 721     669      29
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     678     710    1015    1047    1419 
   Size:        678      32     305      32     372

  Fragments arranged by size: 

                 678     372     305      32      32
 
MluI A'CGCG_T

Cuts at:      0    1083    1419 
   Size:       1083     336
 
MscI TGG'CCA

Cuts at:      0     934    1419 
   Size:        934     485
 
MslI CAynn'nnrTG

Cuts at:      0     269     656    1136    1189    1419 
   Size:        269     387     480      53     230

  Fragments arranged by size: 

                 480     387     269     230      53
 
MspA1I CmG'CkG

Cuts at:      0      16     220     327     416     452     836     987    1021 
   Size:         16     204     107      89      36     384     151      34

Cuts at:   1021    1307    1419 
   Size:        286     112

  Fragments arranged by size: 

                 384     286     204     151     112     107      89      36
                  34      16
 
NaeI GCC'GGC

Cuts at:      0      28    1419 
   Size:         28    1391
 
NgoMIV G'CCGG_C

Cuts at:      0      26    1419 
   Size:         26    1393
 
NspI r_CATG'y

Cuts at:      0     214     661    1419 
   Size:        214     447     758

  Fragments arranged by size: 

                 758     447     214
 
PasI CC'CwG_GG

Cuts at:      0     782    1377    1419 
   Size:        782     595      42

  Fragments arranged by size: 

                 782     595      42
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     257     384    1419 
   Size:        257     127    1035

  Fragments arranged by size: 

                1035     257     127
 
PfoI T'CCnGG_A

Cuts at:      0     317    1419 
   Size:        317    1102
 
PspOMI G'GGCC_C

Cuts at:      0     435    1233    1411    1419 
   Size:        435     798     178       8

  Fragments arranged by size: 

                 798     435     178       8
 
PstI C_TGCA'G

Cuts at:      0      56     146     428     470     485     500     758     827 
   Size:         56      90     282      42      15      15     258      69

Cuts at:    827     862     920    1191    1366    1419 
   Size:         35      58     271     175      53

  Fragments arranged by size: 

                 282     271     258     175      90      69      58      56
                  53      42      35      15      15
 
PvuII CAG'CTG

Cuts at:      0     220     327    1419 
   Size:        220     107    1092

  Fragments arranged by size: 

                1092     220     107
 
SacII CC_GC'GG

Cuts at:      0     453     837    1419 
   Size:        453     384     582

  Fragments arranged by size: 

                 582     453     384
 
SbfI CC_TGCA'GG

Cuts at:      0     146     428     485     920    1419 
   Size:        146     282      57     435     499

  Fragments arranged by size: 

                 499     435     282     146      57
 
ScaI AGT'ACT

Cuts at:      0    1037    1419 
   Size:       1037     382
 
SfcI C'TryA_G

Cuts at:      0      52     142     424     466     481     496     754     823 
   Size:         52      90     282      42      15      15     258      69

Cuts at:    823     858     916    1187    1362    1419 
   Size:         35      58     271     175      57

  Fragments arranged by size: 

                 282     271     258     175      90      69      58      57
                  52      42      35      15      15
 
SmaI CCC'GGG

Cuts at:      0     440    1419 
   Size:        440     979
 
SphI G_CATG'C

Cuts at:      0     661    1419 
   Size:        661     758
 
StuI AGG'CCT

Cuts at:      0      58    1030    1419 
   Size:         58     972     389

  Fragments arranged by size: 

                 972     389      58
 
StyI C'CwwG_G

Cuts at:      0     110     672    1419 
   Size:        110     562     747

  Fragments arranged by size: 

                 747     562     110
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      30     774    1419 
   Size:         30     744     645

  Fragments arranged by size: 

                 744     645      30
 
TatI w'GTAC_w

Cuts at:      0    1035    1419 
   Size:       1035     384
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     533     565    1205    1237    1419 
   Size:        533      32     640      32     182

  Fragments arranged by size: 

                 640     533     182      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     732     815    1419 
   Size:        732      83     604

  Fragments arranged by size: 

                 732     604      83
 
XmaI C'CCGG_G

Cuts at:      0     438    1419 
   Size:        438     981
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AleI     AlfI    AlwNI     ApaI
    ApaLI     AvaI     BanI    BanII     BbsI    BbvCI     BglI     BlpI
 Bme1580I     BpmI   Bpu10I    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII   BstAPI   Bsu36I     BtgI     BtsI     EaeI
     EarI  Eco57MI EcoO109I     FalI    HaeII    Hin4I     MluI     MscI
     MslI   MspA1I     NaeI   NgoMIV     NspI     PasI    PflMI     PfoI
   PspOMI     PstI    PvuII    SacII     SbfI     ScaI     SfcI     SmaI
     SphI     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AloI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsiWI     BsmI    BspEI
    BsrGI    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MmeI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII
     SacI     SalI    SanDI     SapI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmlI    SnaBI     SpeI     SrfI     SspI     SwaI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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