Restriction map for pF1KB5225
 (Linear) (Six-base) MAPSORT of: KIBB5225.seq  Check: 9774  from: 1  to: 2103



KIBB5225.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     851    1024    2103 
   Size:        851     173    1079

  Fragments arranged by size: 

                1079     851     173
 
AatII G_ACGT'C

Cuts at:      0     368    1824    2103 
   Size:        368    1456     279

  Fragments arranged by size: 

                1456     368     279
 
Acc65I G'GTAC_C

Cuts at:      0    1199    2103 
   Size:       1199     904
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     592     613     979    1141    1562    2103 
   Size:        592      21     366     162     421     541

  Fragments arranged by size: 

                 592     541     421     366     162      21
 
AflIII A'CryG_T

Cuts at:      0    1427    2103 
   Size:       1427     676
 
AleI CACnn'nnGTG

Cuts at:      0    1507    2103 
   Size:       1507     596
 
AlwNI CAG_nnn'CTG

Cuts at:      0     114    1606    2103 
   Size:        114    1492     497

  Fragments arranged by size: 

                1492     497     114
 
ApaI G_GGCC'C

Cuts at:      0    1137    2103 
   Size:       1137     966
 
ApoI r'AATT_y

Cuts at:      0     492    2103 
   Size:        492    1611
 
AvaI C'yCGr_G

Cuts at:      0     525    1177    2103 
   Size:        525     652     926

  Fragments arranged by size: 

                 926     652     525
 
AvrII C'CTAG_G

Cuts at:      0     513    2103 
   Size:        513    1590
 
BanI G'GyrC_C

Cuts at:      0    1036    1199    1611    1704    1895    2103 
   Size:       1036     163     412      93     191     208

  Fragments arranged by size: 

                1036     412     208     191     163      93
 
BanII G_rGCy'C

Cuts at:      0     730    1137    1848    2022    2103 
   Size:        730     407     711     174      81

  Fragments arranged by size: 

                 730     711     407     174      81
 
BbeI G_GCGC'C

Cuts at:      0    1708    1899    2103 
   Size:       1708     191     204

  Fragments arranged by size: 

                1708     204     191
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1340    2103 
   Size:       1340     763
 
BbvCI CC'TCA_GC

Cuts at:      0     754    1186    1883    2103 
   Size:        754     432     697     220

  Fragments arranged by size: 

                 754     697     432     220
 
BglI GCCn_nnn'nGGC

Cuts at:      0     130     518    1713    1893    2026    2103 
   Size:        130     388    1195     180     133      77

  Fragments arranged by size: 

                1195     388     180     133     130      77
 
BlpI GC'TnA_GC

Cuts at:      0     749    2103 
   Size:        749    1354
 
Bme1580I G_kGCm'C

Cuts at:      0    1041    1137    1514    2103 
   Size:       1041      96     377     589

  Fragments arranged by size: 

                1041     589     377      96
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1663    1954    2103 
   Size:       1663     291     149

  Fragments arranged by size: 

                1663     291     149
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1029    2103 
   Size:       1029    1074
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     376     418     712    1021    1430    1441    1532 
   Size:        136     240      42     294     309     409      11      91

Cuts at:   1532    1538    1745    2103 
   Size:          6     207     358

  Fragments arranged by size: 

                 409     358     309     294     240     207     136      91
                  42      11       6
 
Bpu10I CC'TnA_GC

Cuts at:      0     754    1186    1883    2103 
   Size:        754     432     697     220

  Fragments arranged by size: 

                 754     697     432     220
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     952    1027    2103 
   Size:        448     504      75    1076

  Fragments arranged by size: 

                1076     504     448      75
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      61     931    1743    2089    2103 
   Size:         61     870     812     346      14

  Fragments arranged by size: 

                 870     812     346      61      14
 
BsaBI GATnn'nnATC

Cuts at:      0     710    2103 
   Size:        710    1393
 
BsaHI Gr'CG_yC

Cuts at:      0     365    1367    1705    1821    1896    2103 
   Size:        365    1002     338     116      75     207

  Fragments arranged by size: 

                1002     365     338     207     116      75
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1518    1548    2103 
   Size:       1518      30     555

  Fragments arranged by size: 

                1518     555      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     409     412     592     595     598     601    1018    1246 
   Size:        409       3     180       3       3       3     417     228

Cuts at:   1246    1743    2030    2103 
   Size:        497     287      73

  Fragments arranged by size: 

                 497     417     409     287     228     180      73       3
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     852     943    1258    1325    1518    1566    1924    1954 
   Size:        852      91     315      67     193      48     358      30

Cuts at:   1954    2052    2103 
   Size:         98      51

  Fragments arranged by size: 

                 852     358     315     193      98      91      67      51
                  48      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       1    1182    1964    2103 
   Size:          1    1181     782     139

  Fragments arranged by size: 

                1181     782     139       1
 
BsiHKAI G_wGCw'C

Cuts at:      0     730    2103 
   Size:        730    1373
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     559     691     970    2103 
   Size:        559     132     279    1133

  Fragments arranged by size: 

                1133     559     279     132
 
Bsp1286I G_dGCh'C

Cuts at:      0     730    1041    1137    1514    1848    2022    2103 
   Size:        730     311      96     377     334     174      81

  Fragments arranged by size: 

                 730     377     334     311     174      96      81
 
BspHI T'CATG_A

Cuts at:      0     890    2103 
   Size:        890    1213
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      90     101     707     851    1024    2103 
   Size:         90      11     606     144     173    1079

  Fragments arranged by size: 

                1079     606     173     144      90      11
 
BsrBI CCG'CTC

Cuts at:      0     182    1751    2103 
   Size:        182    1569     352

  Fragments arranged by size: 

                1569     352     182
 
BsrDI GCAATG_nn'

Cuts at:      0     616    2103 
   Size:        616    1487
 
BsrFI r'CCGG_y

Cuts at:      0     120    1904    2103 
   Size:        120    1784     199

  Fragments arranged by size: 

                1784     199     120
 
BssSI C'ACGA_G

Cuts at:      0     442    2103 
   Size:        442    1661
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      24    2103 
   Size:         24    2079
 
BstEII G'GTnAC_C

Cuts at:      0    1638    2103 
   Size:       1638     465
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     240     561    1278    1359    2103 
   Size:        240     321     717      81     744

  Fragments arranged by size: 

                 744     717     321     240      81
 
Bsu36I CC'TnA_GG

Cuts at:      0    1195    2024    2103 
   Size:       1195     829      79

  Fragments arranged by size: 

                1195     829      79
 
BtgI C'CryG_G

Cuts at:      0     548     765    2103 
   Size:        548     217    1338

  Fragments arranged by size: 

                1338     548     217
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     697    1251    2103 
   Size:        697     554     852

  Fragments arranged by size: 

                 852     697     554
 
BtsI GCAGTG_nn'

Cuts at:      0    1502    2103 
   Size:       1502     601
 
DraIII CAC_nnn'GTG

Cuts at:      0    1029    2103 
   Size:       1029    1074
 
EaeI y'GGCC_r

Cuts at:      0     122     335     551     768    1226    1297    2103 
   Size:        122     213     216     217     458      71     806

  Fragments arranged by size: 

                 806     458     217     216     213     122      71
 
EarI CTCTTCn'nnn_

Cuts at:      0       1     589     914     934    1408    1444    2103 
   Size:          1     588     325      20     474      36     659

  Fragments arranged by size: 

                 659     588     474     325      36      20       1
 
EcoICRI GAG'CTC

Cuts at:      0     728    2103 
   Size:        728    1375
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     376     418     592     613     712     979    1021 
   Size:        136     240      42     174      21      99     267      42

Cuts at:   1021    1141    1430    1441    1532    1538    1562    1745    2103 
   Size:        120     289      11      91       6      24     183     358

  Fragments arranged by size: 

                 358     289     267     240     183     174     136     120
                  99      91      42      42      24      21      11       6
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      85      97    1153    2103 
   Size:         85      12    1056     950

  Fragments arranged by size: 

                1056     950      85      12
 
EcoO109I rG'GnC_Cy

Cuts at:      0      90     947    1133    1146    1282    1799    2040    2103 
   Size:         90     857     186      13     136     517     241      63

  Fragments arranged by size: 

                 857     517     241     186     136      90      63      13
            
 
EcoRI G'AATT_C

Cuts at:      0     492    2103 
   Size:        492    1611
 
HaeII r_GCGC'y

Cuts at:      0    1381    1701    1708    1899    2103 
   Size:       1381     320       7     191     204

  Fragments arranged by size: 

                1381     320     204     191       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1324    1356    2103 
   Size:       1324      32     747

  Fragments arranged by size: 

                1324     747      32
 
HindIII A'AGCT_T

Cuts at:      0     211    2103 
   Size:        211    1892
 
KasI G'GCGC_C

Cuts at:      0    1704    1895    2103 
   Size:       1704     191     208

  Fragments arranged by size: 

                1704     208     191
 
KpnI G_GTAC'C

Cuts at:      0    1203    2103 
   Size:       1203     900
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     473    1040    2103 
   Size:        473     567    1063

  Fragments arranged by size: 

                1063     567     473
 
MscI TGG'CCA

Cuts at:      0     553    1299    2103 
   Size:        553     746     804

  Fragments arranged by size: 

                 804     746     553
 
MslI CAynn'nnrTG

Cuts at:      0     238    1357    1507    2103 
   Size:        238    1119     150     596

  Fragments arranged by size: 

                1119     596     238     150
 
MspA1I CmG'CkG

Cuts at:      0      15      33      73     111     321    1329    1447    1663 
   Size:         15      18      40      38     210    1008     118     216

Cuts at:   1663    2103 
   Size:        440

  Fragments arranged by size: 

                1008     440     216     210     118      40      38      18
                  15
 
NaeI GCC'GGC

Cuts at:      0     122    1906    2103 
   Size:        122    1784     197

  Fragments arranged by size: 

                1784     197     122
 
NarI GG'CG_CC

Cuts at:      0    1705    1896    2103 
   Size:       1705     191     207

  Fragments arranged by size: 

                1705     207     191
 
NcoI C'CATG_G

Cuts at:      0     548    2103 
   Size:        548    1555
 
NgoMIV G'CCGG_C

Cuts at:      0     120    1904    2103 
   Size:        120    1784     199

  Fragments arranged by size: 

                1784     199     120
 
NspI r_CATG'y

Cuts at:      0      27    2103 
   Size:         27    2076
 
PasI CC'CwG_GG

Cuts at:      0     812    1795    2103 
   Size:        812     983     308

  Fragments arranged by size: 

                 983     812     308
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     999    1362    2103 
   Size:        999     363     741

  Fragments arranged by size: 

                 999     741     363
 
PfoI T'CCnGG_A

Cuts at:      0     689    1457    2103 
   Size:        689     768     646

  Fragments arranged by size: 

                 768     689     646
 
PpuMI rG'GwC_Cy

Cuts at:      0      90    1282    1799    2040    2103 
   Size:         90    1192     517     241      63

  Fragments arranged by size: 

                1192     517     241      90      63
 
PspOMI G'GGCC_C

Cuts at:      0    1133    2103 
   Size:       1133     970
 
PstI C_TGCA'G

Cuts at:      0     101     161     173     374    1145    2103 
   Size:        101      60      12     201     771     958

  Fragments arranged by size: 

                 958     771     201     101      60      12
 
PvuII CAG'CTG

Cuts at:      0      15      33     321    1329    1447    1663    2103 
   Size:         15      18     288    1008     118     216     440

  Fragments arranged by size: 

                1008     440     288     216     118      18      15
 
SacI G_AGCT'C

Cuts at:      0     730    2103 
   Size:        730    1373
 
SanDI GG'GwC_CC

Cuts at:      0    1282    1799    2040    2103 
   Size:       1282     517     241      63

  Fragments arranged by size: 

                1282     517     241      63
 
ScaI AGT'ACT

Cuts at:      0     625    2103 
   Size:        625    1478
 
SfcI C'TryA_G

Cuts at:      0      97     157     169     370    1141    2103 
   Size:         97      60      12     201     771     962

  Fragments arranged by size: 

                 962     771     201      97      60      12
 
SfoI GGC'GCC

Cuts at:      0    1706    1897    2103 
   Size:       1706     191     206

  Fragments arranged by size: 

                1706     206     191
 
SmaI CCC'GGG

Cuts at:      0     527    2103 
   Size:        527    1576
 
SmlI C'TyrA_G

Cuts at:      0     463     967    1042    2103 
   Size:        463     504      75    1061

  Fragments arranged by size: 

                1061     504     463      75
 
SphI G_CATG'C

Cuts at:      0      27    2103 
   Size:         27    2076
 
StuI AGG'CCT

Cuts at:      0     163     289     512     883    1436    2103 
   Size:        163     126     223     371     553     667

  Fragments arranged by size: 

                 667     553     371     223     163     126
 
StyI C'CwwG_G

Cuts at:      0     290     513     548     653    1571    1589    1634    2103 
   Size:        290     223      35     105     918      18      45     469

  Fragments arranged by size: 

                 918     469     290     223     105      45      35      18
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      73     817    1272    2103 
   Size:         73     744     455     831

  Fragments arranged by size: 

                 831     744     455      73
 
TatI w'GTAC_w

Cuts at:      0     623    2103 
   Size:        623    1480
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1518    1550    1632    1664    2103 
   Size:       1518      32      82      32     439

  Fragments arranged by size: 

                1518     439      82      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1465    1602    2103 
   Size:       1465     137     501

  Fragments arranged by size: 

                1465     501     137
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1359    2103 
   Size:       1359     744
 
XmaI C'CCGG_G

Cuts at:      0     525    2103 
   Size:        525    1578
 
ZraI GAC'GTC

Cuts at:      0     366    1822    2103 
   Size:        366    1456     281

  Fragments arranged by size: 

                1456     366     281
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI   AflIII     AleI    AlwNI     ApaI
     ApoI     AvaI    AvrII     BanI    BanII     BbeI     BbsI    BbvCI
     BglI     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     BtsI   DraIII
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    HaeII
    Hin4I  HindIII     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PasI    PflMI     PfoI
    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI     ScaI     SfcI
     SfoI     SmaI     SmlI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII     AgeI     AhdI     AlfI     AloI
    ApaLI     AscI     AseI    AsiSI     BaeI    BamHI     BcgI    BciVI
     BclI    BfrBI    BglII    BmgBI     BmtI    BsaAI    BsaWI    BsiEI
    BsiWI     BsmI    BspEI    BsrGI   BssHII    BstBI    BstYI  BstZ17I
     ClaI    CspCI     DraI     DrdI     EagI     EciI    EcoRV     FalI
     FseI     FspI    FspAI   HincII     HpaI     MfeI     MluI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     SapI     SbfI    SexAI     SfiI     SgfI    SgrAI    SnaBI
     SpeI     SrfI     SspI     SwaI     XbaI     XhoI     XmnI
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