Restriction map for pF1KB5018
 (Linear) (Six-base) MAPSORT of: KIBB5018.seq  Check: 4828  from: 1  to: 1086



KIBB5018.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     354     723    1086 
   Size:        354     369     363

  Fragments arranged by size: 

                 369     363     354
 
AatII G_ACGT'C

Cuts at:      0     397    1086 
   Size:        397     689
 
Acc65I G'GTAC_C

Cuts at:      0     250    1086 
   Size:        250     836
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413    1086 
   Size:        413     673
 
AleI CACnn'nnGTG

Cuts at:      0     469    1086 
   Size:        469     617
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     767    1086 
   Size:        767     319
 
AlwNI CAG_nnn'CTG

Cuts at:      0     406     418     780     846    1086 
   Size:        406      12     362      66     240

  Fragments arranged by size: 

                 406     362     240      66      12
 
ApoI r'AATT_y

Cuts at:      0     602    1086 
   Size:        602     484
 
AvaI C'yCGr_G

Cuts at:      0     616    1086 
   Size:        616     470
 
BanI G'GyrC_C

Cuts at:      0     250     821     901    1086 
   Size:        250     571      80     185

  Fragments arranged by size: 

                 571     250     185      80
 
BanII G_rGCy'C

Cuts at:      0     370     481     616     857    1086 
   Size:        370     111     135     241     229

  Fragments arranged by size: 

                 370     241     229     135     111
 
BbeI G_GCGC'C

Cuts at:      0     905    1086 
   Size:        905     181
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     458    1086 
   Size:        458     628
 
BbvCI CC'TCA_GC

Cuts at:      0     255     797    1086 
   Size:        255     542     289

  Fragments arranged by size: 

                 542     289     255
 
BglII A'GATC_T

Cuts at:      0     158    1086 
   Size:        158     928
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     708    1086 
   Size:        708     378
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     712     757    1086 
   Size:        712      45     329

  Fragments arranged by size: 

                 712     329      45
 
Bpu10I CC'TnA_GC

Cuts at:      0     255     797    1086 
   Size:        255     542     289

  Fragments arranged by size: 

                 542     289     255
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183    1086 
   Size:        183     903
 
BsaHI Gr'CG_yC

Cuts at:      0     394     902     946    1086 
   Size:        394     508      44     140

  Fragments arranged by size: 

                 508     394     140      44
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     445     475     496     526    1086 
   Size:        445      30      21      30     560

  Fragments arranged by size: 

                 560     445      30      30      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     928    1086 
   Size:        928     158
 
BseYI C'CCAG_C

Cuts at:      0     780    1086 
   Size:        780     306
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      61     484    1086 
   Size:         61     423     602

  Fragments arranged by size: 

                 602     423      61
 
Bsp1286I G_dGCh'C

Cuts at:      0     370     481     616     857    1086 
   Size:        370     111     135     241     229

  Fragments arranged by size: 

                 370     241     229     135     111
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     354     723    1086 
   Size:        354     369     363

  Fragments arranged by size: 

                 369     363     354
 
BsrBI CCG'CTC

Cuts at:      0     534    1086 
   Size:        534     552
 
BsrFI r'CCGG_y

Cuts at:      0     151     241     663     818    1086 
   Size:        151      90     422     155     268

  Fragments arranged by size: 

                 422     268     155     151      90
 
BstEII G'GTnAC_C

Cuts at:      0     225    1086 
   Size:        225     861
 
BstYI r'GATC_y

Cuts at:      0      47     158    1086 
   Size:         47     111     928

  Fragments arranged by size: 

                 928     111      47
 
Bsu36I CC'TnA_GG

Cuts at:      0     324    1086 
   Size:        324     762
 
BtgI C'CryG_G

Cuts at:      0     125     515     581     668    1086 
   Size:        125     390      66      87     418

  Fragments arranged by size: 

                 418     390     125      87      66
 
ClaI AT'CG_AT

Cuts at:      0     197    1086 
   Size:        197     889
 
EaeI y'GGCC_r

Cuts at:      0       2     243     542     665    1086 
   Size:          2     241     299     123     421

  Fragments arranged by size: 

                 421     299     241     123       2
 
EarI CTCTTCn'nnn_

Cuts at:      0     432    1086 
   Size:        432     654
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     372     886    1086 
   Size:        372     514     200

  Fragments arranged by size: 

                 514     372     200
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     712     757    1086 
   Size:        413     299      45     329

  Fragments arranged by size: 

                 413     329     299      45
 
EcoO109I rG'GnC_Cy

Cuts at:      0     918     981    1086 
   Size:        918      63     105

  Fragments arranged by size: 

                 918     105      63
 
EcoRI G'AATT_C

Cuts at:      0     602    1086 
   Size:        602     484
 
HaeII r_GCGC'y

Cuts at:      0     905    1086 
   Size:        905     181
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     125     157     394     426     445     477     496     521 
   Size:        125      32     237      32      19      32      19      25

Cuts at:    521     528     553    1086 
   Size:          7      25     533

  Fragments arranged by size: 

                 533     237     125      32      32      32      25      25
                  19      19       7
 
KasI G'GCGC_C

Cuts at:      0     901    1086 
   Size:        901     185
 
KpnI G_GTAC'C

Cuts at:      0     254    1086 
   Size:        254     832
 
MslI CAynn'nnrTG

Cuts at:      0     469     514     841     940    1086 
   Size:        469      45     327      99     146

  Fragments arranged by size: 

                 469     327     146      99      45
 
MspA1I CmG'CkG

Cuts at:      0     283     440     780    1086 
   Size:        283     157     340     306

  Fragments arranged by size: 

                 340     306     283     157
 
NaeI GCC'GGC

Cuts at:      0     153     243     665     820    1086 
   Size:        153      90     422     155     266

  Fragments arranged by size: 

                 422     266     155     153      90
 
NarI GG'CG_CC

Cuts at:      0     902    1086 
   Size:        902     184
 
NcoI C'CATG_G

Cuts at:      0     515    1086 
   Size:        515     571
 
NgoMIV G'CCGG_C

Cuts at:      0     151     241     663     818    1086 
   Size:        151      90     422     155     268

  Fragments arranged by size: 

                 422     268     155     151      90
 
NspI r_CATG'y

Cuts at:      0     333     729    1086 
   Size:        333     396     357

  Fragments arranged by size: 

                 396     357     333
 
PasI CC'CwG_GG

Cuts at:      0     859     984    1086 
   Size:        859     125     102

  Fragments arranged by size: 

                 859     125     102
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     780    1086 
   Size:        780     306
 
PfoI T'CCnGG_A

Cuts at:      0     638    1086 
   Size:        638     448
 
PstI C_TGCA'G

Cuts at:      0      32    1086 
   Size:         32    1054
 
PvuII CAG'CTG

Cuts at:      0     283     780    1086 
   Size:        283     497     306

  Fragments arranged by size: 

                 497     306     283
 
SfcI C'TryA_G

Cuts at:      0      28     965    1086 
   Size:         28     937     121

  Fragments arranged by size: 

                 937     121      28
 
SfoI GGC'GCC

Cuts at:      0     903    1086 
   Size:        903     183
 
SmlI C'TyrA_G

Cuts at:      0     162    1086 
   Size:        162     924
 
StuI AGG'CCT

Cuts at:      0     271    1015    1086 
   Size:        271     744      71

  Fragments arranged by size: 

                 744     271      71
 
StyI C'CwwG_G

Cuts at:      0     515    1086 
   Size:        515     571
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     291    1086 
   Size:        291     795
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     588     620    1086 
   Size:        588      32     466

  Fragments arranged by size: 

                 588     466      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     172    1086 
   Size:        172     914
 
ZraI GAC'GTC

Cuts at:      0     395    1086 
   Size:        395     691
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI     AleI     AlfI    AlwNI     ApoI
     AvaI     BanI    BanII     BbeI     BbsI    BbvCI    BglII     BmrI
     BpmI   Bpu10I    BpuEI    BsaHI    BsaXI    BseRI    BseYI    BsmBI
 Bsp1286I    BspMI    BsrBI    BsrFI   BstEII    BstYI   Bsu36I     BtgI
     ClaI     EaeI     EarI     EciI  Eco57MI EcoO109I    EcoRI    HaeII
    Hin4I     KasI     KpnI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PasI    PflMI     PfoI     PstI    PvuII     SfcI
     SfoI     SmlI     StuI     StyI    TaqII     TstI  Tth111I     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmtI     BplI     BsaI    BsaAI    BsaBI    BsaWI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI  BstZ17I    BtgZI     BtsI    CspCI
     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     MfeI     MluI
     MmeI     MscI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI     XbaI
     XcmI     XhoI     XmaI     XmnI
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