Restriction map for
pF1KB4990
(Circular) (Six-base) MAPSORT of: pF1KB4990.seq Check: 4717 from: 1 to: 4592
pF1KB4990 4592 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 1630 1630
Size: 4592
Acc65I G'GTAC_C
Cuts at: 1608 1608
Size: 4592
AclI AA'CG_TT
Cuts at: 4432 4432
Size: 4592
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1369 2417 2849 3202 3864 1369
Size: 1048 432 353 662 2097
Fragments arranged by size:
2097 1048 662 432 353
AfeI AGC'GCT
Cuts at: 137 137
Size: 4592
AflIII A'CryG_T
Cuts at: 1944 3744 4025 1944
Size: 1800 281 2511
Fragments arranged by size:
2511 1800 281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1060 1060
Size: 4592
AlwNI CAG_nnn'CTG
Cuts at: 369 1582 1897 3335 369
Size: 1213 315 1438 1626
Fragments arranged by size:
1626 1438 1213 315
ApaI G_GGCC'C
Cuts at: 126 963 126
Size: 837 3755
ApaLI G'TGCA_C
Cuts at: 3430 3430
Size: 4592
ApoI r'AATT_y
Cuts at: 1596 3945 4201 1596
Size: 2349 256 1987
Fragments arranged by size:
2349 1987 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4592
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4592
AvaI C'yCGr_G
Cuts at: 503 1612 503
Size: 1109 3483
BamHI G'GATC_C
Cuts at: 507 771 1617 507
Size: 264 846 3482
Fragments arranged by size:
3482 846 264
BanI G'GyrC_C
Cuts at: 990 1129 1608 2022 2270 2305 990
Size: 139 479 414 248 35 3277
Fragments arranged by size:
3277 479 414 248 139 35
BanII G_rGCy'C
Cuts at: 126 347 500 872 963 1054 1606 2636 126
Size: 221 153 372 91 91 552 1030 2082
Fragments arranged by size:
2082 1030 552 372 221 153 91 91
BbeI G_GCGC'C
Cuts at: 2026 2274 2026
Size: 248 4344
BbsI GAAGACnn'nnnn_
Cuts at: 1357 1357
Size: 4592
BbvCI CC'TCA_GC
Cuts at: 462 467 1015 462
Size: 5 548 4039
Fragments arranged by size:
4039 548 5
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1298 1332 1298
Size: 34 4558
BciVI GTATCCnnnnn_n'
Cuts at: 2490 3546 4592 2490
Size: 1056 1046 2490
Fragments arranged by size:
2490 1056 1046
BclI T'GATC_A
Cuts at: 2112 2112
Size: 4592
BglI GCCn_nnn'nGGC
Cuts at: 1237 1237
Size: 4592
BglII A'GATC_T
Cuts at: 656 2107 4144 656
Size: 1451 2037 1104
Fragments arranged by size:
2037 1451 1104
BlpI GC'TnA_GC
Cuts at: 1048 1704 1048
Size: 656 3936
Bme1580I G_kGCm'C
Cuts at: 126 963 995 2217 2310 3434 126
Size: 837 32 1222 93 1124 1284
Fragments arranged by size:
1284 1222 1124 837 93 32
BmrI ACTGGGnnnn_n'
Cuts at: 1804 1885 2220 4364 1804
Size: 81 335 2144 2032
Fragments arranged by size:
2144 2032 335 81
BmtI G_CTAG'C
Cuts at: 1154 1154
Size: 4592
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2959 2959
Size: 4592
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 253 265 514 543 864 1102 1333 220
Size: 33 12 249 29 321 238 231 3479
Fragments arranged by size:
3479 321 249 238 231 33 29 12
Bpu10I CC'TnA_GC
Cuts at: 462 467 1015 462
Size: 5 548 4039
Fragments arranged by size:
4039 548 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1768 2647 3361 3659 1768
Size: 879 714 298 2701
Fragments arranged by size:
2701 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 4296 4296
Size: 4592
BsaAI yAC'GTr
Cuts at: 1945 1945
Size: 4592
BsaBI GATnn'nnATC
Cuts at: 6 2106 4143 6
Size: 2100 2037 455
Fragments arranged by size:
2100 2037 455
BsaHI Gr'CG_yC
Cuts at: 1217 1552 2023 2271 2973 4469 1217
Size: 335 471 248 702 1496 1340
Fragments arranged by size:
1496 1340 702 471 335 248
BsaWI w'CCGG_w
Cuts at: 261 410 1781 2302 2980 3391 3538 3812 261
Size: 149 1371 521 678 411 147 274 1041
Fragments arranged by size:
1371 1041 678 521 411 274 149 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 634 634
Size: 4592
BseYI C'CCAG_C
Cuts at: 872 1053 2017 2775 3440 3848 872
Size: 181 964 758 665 408 1616
Fragments arranged by size:
1616 964 758 665 408 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1095 1292 1429 1095
Size: 197 137 4258
Fragments arranged by size:
4258 197 137
BsiEI CG_ry'CG
Cuts at: 104 2134 2180 3410 104
Size: 2030 46 1230 1286
Fragments arranged by size:
2030 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 347 500 1606 2384 2574 3434 347
Size: 153 1106 778 190 860 1505
Fragments arranged by size:
1505 1106 860 778 190 153
BsmI GAATG_Cn'
Cuts at: 569 569
Size: 4592
BsmBI CGTCTCn'nnnn_
Cuts at: 1228 1228
Size: 4592
Bsp1286I G_dGCh'C
Cuts at: 126 347 500 872 963 995 1054 1606 2217
Size: 221 153 372 91 32 59 552 611
Cuts at: 2217 2310 2384 2574 2636 3434 126
Size: 93 74 190 62 798 1284
Fragments arranged by size:
1284 798 611 552 372 221 190 153
93 91 74 62 59 32
BspEI T'CCGG_A
Cuts at: 261 410 261
Size: 149 4443
BspHI T'CATG_A
Cuts at: 764 3024 764
Size: 2260 2332
BspMI ACCTGCnnnn'nnnn_
Cuts at: 275 1271 1283 1642 1856 2158 2539 275
Size: 996 12 359 214 302 381 2328
Fragments arranged by size:
2328 996 381 359 302 214 12
BsrBI CCG'CTC
Cuts at: 50 891 1392 2884 2938 4422 4587 50
Size: 841 501 1492 54 1484 165 55
Fragments arranged by size:
1492 1484 841 501 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1160 2504 1160
Size: 1344 3248
BsrFI r'CCGG_y
Cuts at: 1532 2590 2771 1532
Size: 1058 181 3353
Fragments arranged by size:
3353 1058 181
BssSI C'ACGA_G
Cuts at: 2863 3571 2863
Size: 708 3884
BstAPI GCAn_nnn'nTGC
Cuts at: 972 1969 972
Size: 997 3595
BstBI TT'CG_AA
Cuts at: 2953 2953
Size: 4592
BstXI CCAn_nnnn'nTGG
Cuts at: 198 795 1167 198
Size: 597 372 3623
Fragments arranged by size:
3623 597 372
BstYI r'GATC_y
Cuts at: 1 176 507 656 771 1412 1617 2084 2107
Size: 175 331 149 115 641 205 467 23
Cuts at: 2107 2442 2688 2994 3006 3092 4144 1
Size: 335 246 306 12 86 1052 449
Fragments arranged by size:
1052 641 467 449 335 331 306 246
205 175 149 115 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 627 1187 1424 627
Size: 560 237 3795
Fragments arranged by size:
3795 560 237
BtgI C'CryG_G
Cuts at: 107 935 107
Size: 828 3764
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2542 2723 2886 2542
Size: 181 163 4248
Fragments arranged by size:
4248 181 163
BtsI GCAGTG_nn'
Cuts at: 869 1064 1145 1556 1835 869
Size: 195 81 411 279 3626
Fragments arranged by size:
3626 411 279 195 81
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1011 1046 1011
Size: 35 4557
DraI TTT'AAA
Cuts at: 1591 1591
Size: 4592
DrdI GACnn_nn'nnGTC
Cuts at: 2298 3642 2298
Size: 1344 3248
EaeI y'GGCC_r
Cuts at: 2177 2351 2742 2769 4114 2177
Size: 174 391 27 1345 2655
Fragments arranged by size:
2655 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2177 2177
Size: 4592
EarI CTCTTCn'nnn_
Cuts at: 121 133 881 2615 2825 121
Size: 12 748 1734 210 1888
Fragments arranged by size:
1888 1734 748 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2800 3542 3688 4402 2800
Size: 742 146 714 2990
Fragments arranged by size:
2990 742 714 146
EcoICRI GAG'CTC
Cuts at: 345 498 1604 345
Size: 153 1106 3333
Fragments arranged by size:
3333 1106 153
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 220 253 265 514 543 864 1102 1333 1369
Size: 33 12 249 29 321 238 231 36
Cuts at: 1369 2417 2849 3202 3864 220
Size: 1048 432 353 662 948
Fragments arranged by size:
1048 948 662 432 353 321 249 238
231 36 33 29 12
EcoNI CCTnn'n_nnAGG
Cuts at: 766 766
Size: 4592
EcoO109I rG'GnC_Cy
Cuts at: 122 123 815 959 960 1092 1254 1341 1731
Size: 1 692 144 1 132 162 87 390
Cuts at: 1731 122
Size: 2983
Fragments arranged by size:
2983 692 390 162 144 132 87 1
1
EcoRI G'AATT_C
Cuts at: 1596 1596
Size: 4592
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 4592
FspI TGC'GCA
Cuts at: 724 1261 1279 2373 724
Size: 537 18 1094 2943
Fragments arranged by size:
2943 1094 537 18
HaeII r_GCGC'y
Cuts at: 139 199 1232 2026 2274 3504 4275 139
Size: 60 1033 794 248 1230 771 456
Fragments arranged by size:
1230 1033 794 771 456 248 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 407 439 460 492 595 627 2434 2464 2466
Size: 32 21 32 103 32 1807 30 2
Cuts at: 2466 2496 407
Size: 30 2503
Fragments arranged by size:
2503 1807 103 32 32 32 30 30
21 2
HincII GTy'rAC
Cuts at: 1631 1631
Size: 4592
KasI G'GCGC_C
Cuts at: 2022 2270 2022
Size: 248 4344
KpnI G_GTAC'C
Cuts at: 1612 1612
Size: 4592
MluI A'CGCG_T
Cuts at: 4025 4025
Size: 4592
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1322 3351 3535 1322
Size: 2029 184 2379
Fragments arranged by size:
2379 2029 184
MscI TGG'CCA
Cuts at: 2353 2353
Size: 4592
MslI CAynn'nnrTG
Cuts at: 1210 2581 2708 1210
Size: 1371 127 3094
Fragments arranged by size:
3094 1371 127
MspA1I CmG'CkG
Cuts at: 420 466 514 876 969 1197 1269 1582 1703
Size: 46 48 362 93 228 72 313 121
Cuts at: 1703 2017 2377 3161 3406 420
Size: 314 360 784 245 1606
Fragments arranged by size:
1606 784 362 360 314 313 245 228
121 93 72 48 46
NaeI GCC'GGC
Cuts at: 2773 2773
Size: 4592
NarI GG'CG_CC
Cuts at: 2023 2271 2023
Size: 248 4344
NcoI C'CATG_G
Cuts at: 107 935 107
Size: 828 3764
NgoMIV G'CCGG_C
Cuts at: 2771 2771
Size: 4592
NheI G'CTAG_C
Cuts at: 1150 1150
Size: 4592
NspI r_CATG'y
Cuts at: 157 1011 1645 2145 3748 157
Size: 854 634 500 1603 1001
Fragments arranged by size:
1603 1001 854 634 500
PciI A'CATG_T
Cuts at: 3744 3744
Size: 4592
PflMI CCAn_nnn'nTGG
Cuts at: 1002 1002
Size: 4592
PfoI T'CCnGG_A
Cuts at: 704 704
Size: 4592
PmeI GTTT'AAAC
Cuts at: 1591 1591
Size: 4592
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3038 3070 3038
Size: 32 4560
PpuMI rG'GwC_Cy
Cuts at: 1092 1092
Size: 4592
PshAI GACnn'nnGTC
Cuts at: 1220 1220
Size: 4592
PspOMI G'GGCC_C
Cuts at: 122 959 122
Size: 837 3755
PstI C_TGCA'G
Cuts at: 881 968 1364 1639 2324 881
Size: 87 396 275 685 3149
Fragments arranged by size:
3149 685 396 275 87
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4592
PvuII CAG'CTG
Cuts at: 420 466 876 969 1197 1269 1582 2017 2377
Size: 46 410 93 228 72 313 435 360
Cuts at: 2377 420
Size: 2635
Fragments arranged by size:
2635 435 410 360 313 228 93 72
46
RsrII CG'GwC_CG
Cuts at: 2787 2787
Size: 4592
SacI G_AGCT'C
Cuts at: 347 500 1606 347
Size: 153 1106 3333
Fragments arranged by size:
3333 1106 153
SalI G'TCGA_C
Cuts at: 1629 1629
Size: 4592
SapI GCTCTTCn'nnn_
Cuts at: 881 2615 2825 881
Size: 1734 210 2648
Fragments arranged by size:
2648 1734 210
SbfI CC_TGCA'GG
Cuts at: 1639 1639
Size: 4592
ScaI AGT'ACT
Cuts at: 4109 4109
Size: 4592
SfcI C'TryA_G
Cuts at: 33 877 964 1360 1635 2320 3288 3479 33
Size: 844 87 396 275 685 968 191 1146
Fragments arranged by size:
1146 968 844 685 396 275 191 87
SfoI GGC'GCC
Cuts at: 2024 2272 2024
Size: 248 4344
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4592
SmaI CCC'GGG
Cuts at: 505 1614 505
Size: 1109 3483
SmlI C'TyrA_G
Cuts at: 1747 2662 3376 3638 1747
Size: 915 714 262 2701
Fragments arranged by size:
2701 915 714 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4592
SphI G_CATG'C
Cuts at: 1645 2145 1645
Size: 500 4092
StuI AGG'CCT
Cuts at: 550 550
Size: 4592
StyI C'CwwG_G
Cuts at: 107 191 395 935 1726 2080 107
Size: 84 204 540 791 354 2619
Fragments arranged by size:
2619 791 540 354 204 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2175 2308 2767 2976 2175
Size: 133 459 209 3791
Fragments arranged by size:
3791 459 209 133
TatI w'GTAC_w
Cuts at: 4107 4107
Size: 4592
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1386 1418 4109 4141 1386
Size: 32 2691 32 1837
Fragments arranged by size:
2691 1837 32 32
Tth111I GACn'n_nGTC
Cuts at: 2389 2389
Size: 4592
XbaI T'CTAG_A
Cuts at: 1623 1623
Size: 4592
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 393 792 861 1209 2087 393
Size: 399 69 348 878 2898
Fragments arranged by size:
2898 878 399 348 69
XmaI C'CCGG_G
Cuts at: 503 1612 503
Size: 1109 3483
XmnI GAAnn'nnTTC
Cuts at: 1293 1293
Size: 4592
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NheI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AgeI AhdI AleI AloI AscI
AvrII BaeI BfrBI BmgBI BsaXI BsiWI BsrGI BssHII
BstEII BstZ17I ClaI DraIII EcoRV FseI FspAI HindIII
HpaI MfeI NdeI NotI NruI NsiI PacI PasI
PmlI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI