Restriction map for pF1KB4976
 (Linear) (Six-base) MAPSORT of: KIBB4976.seq  Check: 1288  from: 1  to: 1254



KIBB4976.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AccI GT'mk_AC

Cuts at:      0      59     812    1254 
   Size:         59     753     442

  Fragments arranged by size: 

                 753     442      59
 
Acc65I G'GTAC_C

Cuts at:      0    1225    1254 
   Size:       1225      29
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     738    1254 
   Size:        738     516
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1185    1254 
   Size:       1185      69
 
ApaI G_GGCC'C

Cuts at:      0     398    1254 
   Size:        398     856
 
ApoI r'AATT_y

Cuts at:      0     456     483    1254 
   Size:        456      27     771

  Fragments arranged by size: 

                 771     456      27
 
AvaI C'yCGr_G

Cuts at:      0      17     271     989    1254 
   Size:         17     254     718     265

  Fragments arranged by size: 

                 718     265     254      17
 
BamHI G'GATC_C

Cuts at:      0     129    1254 
   Size:        129    1125
 
BanI G'GyrC_C

Cuts at:      0     786     925    1225    1254 
   Size:        786     139     300      29

  Fragments arranged by size: 

                 786     300     139      29
 
BanII G_rGCy'C

Cuts at:      0     398    1254 
   Size:        398     856
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     407     893    1254 
   Size:        407     486     361

  Fragments arranged by size: 

                 486     407     361
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     776     810    1254 
   Size:        776      34     444

  Fragments arranged by size: 

                 776     444      34
 
BglII A'GATC_T

Cuts at:      0     617     695    1008    1254 
   Size:        617      78     313     246

  Fragments arranged by size: 

                 617     313     246      78
 
Bme1580I G_kGCm'C

Cuts at:      0     398     754    1254 
   Size:        398     356     500

  Fragments arranged by size: 

                 500     398     356
 
BmgBI CAC'GTC

Cuts at:      0     657    1026    1254 
   Size:        657     369     228

  Fragments arranged by size: 

                 657     369     228
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     862    1254 
   Size:        862     392
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     547    1254 
   Size:        547     707
 
BsaBI GATnn'nnATC

Cuts at:      0     694    1121    1254 
   Size:        694     427     133

  Fragments arranged by size: 

                 694     427     133
 
BsaHI Gr'CG_yC

Cuts at:      0    1108    1254 
   Size:       1108     146
 
BsaWI w'CCGG_w

Cuts at:      0     503    1254 
   Size:        503     751
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1218    1248    1254 
   Size:       1218      30       6

  Fragments arranged by size: 

                1218      30       6
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     478    1231    1254 
   Size:        478     753      23

  Fragments arranged by size: 

                 753     478      23
 
BseYI C'CCAG_C

Cuts at:      0     321     539     790    1254 
   Size:        321     218     251     464

  Fragments arranged by size: 

                 464     321     251     218
 
BsiHKAI G_wGCw'C

Cuts at:      0     332    1254 
   Size:        332     922
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1099    1254 
   Size:       1099     155
 
Bsp1286I G_dGCh'C

Cuts at:      0     332     398     754    1254 
   Size:        332      66     356     500

  Fragments arranged by size: 

                 500     356     332      66
 
BspEI T'CCGG_A

Cuts at:      0     503    1254 
   Size:        503     751
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     181    1254 
   Size:        181    1073
 
BsrBI CCG'CTC

Cuts at:      0     150    1254 
   Size:        150    1104
 
BsrFI r'CCGG_y

Cuts at:      0     264    1228    1254 
   Size:        264     964      26

  Fragments arranged by size: 

                 964     264      26
 
BssSI C'ACGA_G

Cuts at:      0      97    1254 
   Size:         97    1157
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1026    1254 
   Size:       1026     228
 
BstYI r'GATC_y

Cuts at:      0     129     353     470     617     695     938    1008    1254 
   Size:        129     224     117     147      78     243      70     246

  Fragments arranged by size: 

                 246     243     224     147     129     117      78      70
            
 
BtgI C'CryG_G

Cuts at:      0     627     761    1031    1254 
   Size:        627     134     270     223

  Fragments arranged by size: 

                 627     270     223     134
 
BtsI GCAGTG_nn'

Cuts at:      0     382    1254 
   Size:        382     872
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     205     433    1254 
   Size:        205     228     821

  Fragments arranged by size: 

                 821     228     205
 
EaeI y'GGCC_r

Cuts at:      0     189     242    1028    1254 
   Size:        189      53     786     226

  Fragments arranged by size: 

                 786     226     189      53
 
EarI CTCTTCn'nnn_

Cuts at:      0     761    1208    1254 
   Size:        761     447      46

  Fragments arranged by size: 

                 761     447      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0      14     198     231     394     855    1254 
   Size:         14     184      33     163     461     399

  Fragments arranged by size: 

                 461     399     184     163      33      14
 
EcoRI G'AATT_C

Cuts at:      0     483    1254 
   Size:        483     771
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      93    1254 
   Size:         93    1161
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     623     655    1000    1032    1254 
   Size:        623      32     345      32     222

  Fragments arranged by size: 

                 623     345     222      32      32
 
HincII GTy'rAC

Cuts at:      0     648    1254 
   Size:        648     606
 
KpnI G_GTAC'C

Cuts at:      0    1229    1254 
   Size:       1229      25
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     557    1254 
   Size:        557     697
 
MscI TGG'CCA

Cuts at:      0     244    1254 
   Size:        244    1010
 
MslI CAynn'nnrTG

Cuts at:      0    1051    1254 
   Size:       1051     203
 
NcoI C'CATG_G

Cuts at:      0     627     761    1254 
   Size:        627     134     493

  Fragments arranged by size: 

                 627     493     134
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     886     918    1254 
   Size:        886      32     336

  Fragments arranged by size: 

                 886     336      32
 
PpuMI rG'GwC_Cy

Cuts at:      0      14     198     231    1254 
   Size:         14     184      33    1023

  Fragments arranged by size: 

                1023     184      33      14
 
PspOMI G'GGCC_C

Cuts at:      0     394    1254 
   Size:        394     860
 
PstI C_TGCA'G

Cuts at:      0     302     389    1254 
   Size:        302      87     865

  Fragments arranged by size: 

                 865     302      87
 
SanDI GG'GwC_CC

Cuts at:      0      14    1254 
   Size:         14    1240
 
SfcI C'TryA_G

Cuts at:      0     298     385    1254 
   Size:        298      87     869

  Fragments arranged by size: 

                 869     298      87
 
SmlI C'TyrA_G

Cuts at:      0     877    1254 
   Size:        877     377
 
StuI AGG'CCT

Cuts at:      0     531    1254 
   Size:        531     723
 
StyI C'CwwG_G

Cuts at:      0     627     761     863    1254 
   Size:        627     134     102     391

  Fragments arranged by size: 

                 627     391     134     102
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     804    1254 
   Size:        804     450
 
TatI w'GTAC_w

Cuts at:      0     743    1254 
   Size:        743     511
 

 Enzymes that do cut:

     AccI   Acc65I     AlfI    AlwNI     ApaI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI     BcgI    BglII Bme1580I    BmgBI    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BssSI    BstXI
    BstYI     BtgI     BtsI     DrdI     EaeI     EarI EcoO109I    EcoRI
     FalI    Hin4I   HincII     KpnI     MmeI     MscI     MslI     NcoI
     PpiI    PpuMI   PspOMI     PstI    SanDI     SfcI     SmlI     StuI
     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI     BlpI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI     BsgI    BsiEI
    BsiWI     BsmI    BspHI    BsrDI    BsrGI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRV     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     MfeI     MluI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI
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