Restriction map for pF1KB4862
##################################
# NO CUT ENZYMES (  127 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AflII       
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AviII       AvrII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     CelII       Cfr10I      ClaI        
CpoI        DraI        DraIII      EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MunI        MvnI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tth111I     
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII        1432  GACGTC               GACGT^C
AccI          409  GTAGAC               GT^[AC][GT]AC
AcyI         1432  GACGTC               G[GA]^CG[CT]C
AlwNI        1387  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1408  CCTGAGG              CC^T[ACGT]AGG
Asp700I       387  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         715  GAGCTC               G[AT]GC[AT]^C
BalI         1244  TGGCCA               TGG^CCA
BamHI         323  GGATCC               G^GATCC
BanI         1036  GGCACC               G^G[CT][GA]CC
BcnI          338  CCCGG                CC^[GC]GG
BsaAI        1360  TACGTG               [CT]AC^GT[GA]
BsaHI        1432  GACGTC               G[GA]^CG[CT]C
BsaI          764  GAGACC               GGTCTC(1/5)
BsiHKAI       715  GAGCTC               G[AT]GC[AT]^C
BspLU11I      836  ACATGT               A^CATGT
BspMI        1382  ACCTGC               ACCTGC(4/8)
Bsu36I       1408  CCTGAGG              CC^T[ACGT]AGG
DraII          75  AGGACCT              [GA]G^G[ACGT]CC[CT]
DrdI         1081  GACAAGCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1302  CCGTGG               C^C[GA][CT]GG
EaeI         1244  TGGCCA               [CT]^GGCC[GA]
EarI          712  GAAGAG               CTCTTC(1/4)
Eco81I       1408  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1071  CCTCAATAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       75  AGGACCT              [GA]G^G[ACGT]CC[CT]
EcoT14I      1031  CCAAGG               C^C[AT][AT]GG
HaeII          48  AGCGCC               [GA]GCGC^[CT]
HgaI          690  GACGC                GACGC(5/10)
Hin1I        1432  GACGTC               G[GA]^CG[CT]C
HincII        565  GTCAAC               GT[CT]^[GA]AC
HindII        565  GTCAAC               GT[CT]^[GA]AC
Ksp632I       712  GAAGAG               CTCTTC(1/4)
MluNI        1244  TGGCCA               TGG^CCA
MscI         1244  TGGCCA               TGG^CCA
MslI          837  CATGTGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          338  CCCGG                CC^[GC]GG
PaeR7I        472  CTCGAG               C^TCGAG
PflMI        1247  CCAGGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI          75  AGGACCT              [GA]G^G[AT]CC[CT]
SacI          715  GAGCTC               GAGCT^C
SapI          712  GAAGAGC              GCTCTTC(1/4)
SfaNI        1444  GATGC                GCATC(5/9)
SphI          627  GCATGC               GCATG^C
StyI         1031  CCAAGG               C^C[AT][AT]GG
Van91I       1247  CCAGGAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          516  TCTAGA               T^CTAGA
XcmI         1132  CCAATCCAGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          472  CTCGAG               C^TCGAG
XmnI          387  GAATGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI          391  GAATTC               [GA]^AATT[CT]
             1216  GAATTC              
AfaI         1365  GTAC                 GT^AC
             1401  GTAC                
AflIII        836  ACATGT               A^C[GA][CT]GT
             1361  ACGTGT              
ApoI          391  GAATTC               [GA]^AATT[CT]
             1216  GAATTC              
AvaI          472  CTCGAG               C^[CT]CG[GA]G
              868  CTCGGG              
BanII         661  GAGCCC               G[GA]GC[CT]^C
              715  GAGCTC              
BpmI          772  CTCCAG               CTGGAG(16/14)
              933  CTGGAG              
BseRI         718  CTCCTC               GAGGAG(10/8)
             1411  GAGGAG              
BsgI         1417  CTGCAC               GTGCAG(16/14)
              224  GTGCAG              
BsoBI         472  CTCGAG               C^[CT]CG[GA]G
              868  CTCGGG              
BstAPI       1386  GCAGAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1419  GCACGCCATGC         
CfoI           49  GCGC                 GCG^C
              449  GCGC                
EcoRI         391  GAATTC               G^AATTC
             1216  GAATTC              
HapII         339  CCGG                 C^CGG
             1006  CCGG                
HhaI           49  GCGC                 GCG^C
              449  GCGC                
HinP1I         49  GCGC                 G^CGC
              449  GCGC                
HpaII         339  CCGG                 C^CGG
             1006  CCGG                
MaeII        1361  ACGT                 A^CGT
             1433  ACGT                
MaeIII        823  GTCAC                ^GT[ACGT]AC
             1379  GTGAC               
MspI          339  CCGG                 C^CGG
             1006  CCGG                
NspI          627  GCATGC               [GA]CATG^[CT]
              836  ACATGT              
PvuII        1138  CAGCTG               CAG^CTG
             1194  CAGCTG              
RsaI         1365  GTAC                 GT^AC
             1401  GTAC                
TfiI           16  GATTC                G^A[AT]TC
              583  GAATC               
Tsp45I        823  GTCAC                ^GT[GC]AC
             1379  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AluI        AlwI        AvaII       BbvI        
BfaI        BmyI        BsaJI       BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr13I      
DdeI        DpnII       EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeI        MboI        MboII       MflI        MnlI        
MseI        MspA1I      MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        PstI        Sau3AI      
Sau96I      ScrFI       SfcI        SmlI        TaqI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I     
XhoII       
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