Restriction map for pF1KB4862
 (Linear) (Six-base) MAPSORT of: KIBB4862.seq  Check: 3992  from: 1  to: 1455



KIBB4862.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AatII G_ACGT'C

Cuts at:      0    1436    1455 
   Size:       1436      19
 
AccI GT'mk_AC

Cuts at:      0     410    1455 
   Size:        410    1045
 
AflIII A'CryG_T

Cuts at:      0     836    1361    1455 
   Size:        836     525      94

  Fragments arranged by size: 

                 836     525      94
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     462    1455 
   Size:        462     993
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1392    1455 
   Size:       1392      63
 
ApoI r'AATT_y

Cuts at:      0     391    1216    1455 
   Size:        391     825     239

  Fragments arranged by size: 

                 825     391     239
 
AvaI C'yCGr_G

Cuts at:      0     472     868    1455 
   Size:        472     396     587

  Fragments arranged by size: 

                 587     472     396
 
BamHI G'GATC_C

Cuts at:      0     323    1455 
   Size:        323    1132
 
BanI G'GyrC_C

Cuts at:      0    1036    1455 
   Size:       1036     419
 
BanII G_rGCy'C

Cuts at:      0     665     719    1455 
   Size:        665      54     736

  Fragments arranged by size: 

                 736     665      54
 
Bme1580I G_kGCm'C

Cuts at:      0    1039    1455 
   Size:       1039     416
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     328     378     627     921    1455 
   Size:        328      50     249     294     534

  Fragments arranged by size: 

                 534     328     294     249      50
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     460     738     787    1455 
   Size:        460     278      49     668

  Fragments arranged by size: 

                 668     460     278      49
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     757     954    1455 
   Size:        757     197     501

  Fragments arranged by size: 

                 757     501     197
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     712    1455 
   Size:        254     458     743

  Fragments arranged by size: 

                 743     458     254
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     758    1455 
   Size:        758     697
 
BsaAI yAC'GTr

Cuts at:      0    1362    1455 
   Size:       1362      93
 
BsaHI Gr'CG_yC

Cuts at:      0    1433    1455 
   Size:       1433      22
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     840     870    1455 
   Size:        840      30     585

  Fragments arranged by size: 

                 840     585      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     709    1426    1455 
   Size:        709     717      29

  Fragments arranged by size: 

                 717     709      29
 
BseYI C'CCAG_C

Cuts at:      0      22     570     664    1211    1395    1455 
   Size:         22     548      94     547     184      60

  Fragments arranged by size: 

                 548     547     184      94      60      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245    1402    1455 
   Size:        245    1157      53

  Fragments arranged by size: 

                1157     245      53
 
BsiHKAI G_wGCw'C

Cuts at:      0     719    1455 
   Size:        719     736
 
Bsp1286I G_dGCh'C

Cuts at:      0     665     719    1039    1455 
   Size:        665      54     320     416

  Fragments arranged by size: 

                 665     416     320      54
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1391    1455 
   Size:       1391      64
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1392    1425    1455 
   Size:       1392      33      30

  Fragments arranged by size: 

                1392      33      30
 
BstYI r'GATC_y

Cuts at:      0      96     323     521    1455 
   Size:         96     227     198     934

  Fragments arranged by size: 

                 934     227     198      96
 
Bsu36I CC'TnA_GG

Cuts at:      0    1409    1455 
   Size:       1409      46
 
BtgI C'CryG_G

Cuts at:      0    1302    1455 
   Size:       1302     153
 
BtsI GCAGTG_nn'

Cuts at:      0      68     349    1131    1455 
   Size:         68     281     782     324

  Fragments arranged by size: 

                 782     324     281      68
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1087    1455 
   Size:       1087     368
 
EaeI y'GGCC_r

Cuts at:      0    1244    1455 
   Size:       1244     211
 
EarI CTCTTCn'nnn_

Cuts at:      0     707    1455 
   Size:        707     748
 
EcoICRI GAG'CTC

Cuts at:      0     717    1455 
   Size:        717     738
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     757     954    1455 
   Size:        757     197     501

  Fragments arranged by size: 

                 757     501     197
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1075    1455 
   Size:       1075     380
 
EcoO109I rG'GnC_Cy

Cuts at:      0      76    1455 
   Size:         76    1379
 
EcoRI G'AATT_C

Cuts at:      0     391    1216    1455 
   Size:        391     825     239

  Fragments arranged by size: 

                 825     391     239
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1056    1455 
   Size:       1056     399
 
HaeII r_GCGC'y

Cuts at:      0      52    1455 
   Size:         52    1403
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     159     191     308     340     681     713     733     765 
   Size:        159      32     117      32     341      32      20      32

Cuts at:    765     840     872    1455 
   Size:         75      32     583

  Fragments arranged by size: 

                 583     341     159     117      75      32      32      32
                  32      32      20
 
HincII GTy'rAC

Cuts at:      0     567    1455 
   Size:        567     888
 
MscI TGG'CCA

Cuts at:      0    1246    1455 
   Size:       1246     209
 
MslI CAynn'nnrTG

Cuts at:      0     841    1455 
   Size:        841     614
 
MspA1I CmG'CkG

Cuts at:      0    1140    1196    1348    1455 
   Size:       1140      56     152     107

  Fragments arranged by size: 

                1140     152     107      56
 
NspI r_CATG'y

Cuts at:      0     631     840    1455 
   Size:        631     209     615

  Fragments arranged by size: 

                 631     615     209
 
PasI CC'CwG_GG

Cuts at:      0     453    1455 
   Size:        453    1002
 
PciI A'CATG_T

Cuts at:      0     836    1455 
   Size:        836     619
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1253    1455 
   Size:       1253     202
 
PfoI T'CCnGG_A

Cuts at:      0     931    1455 
   Size:        931     524
 
PpuMI rG'GwC_Cy

Cuts at:      0      76    1455 
   Size:         76    1379
 
PstI C_TGCA'G

Cuts at:      0      63     530     965    1388    1455 
   Size:         63     467     435     423      67

  Fragments arranged by size: 

                 467     435     423      67      63
 
PvuII CAG'CTG

Cuts at:      0    1140    1196    1455 
   Size:       1140      56     259

  Fragments arranged by size: 

                1140     259      56
 
SacI G_AGCT'C

Cuts at:      0     719    1455 
   Size:        719     736
 
SapI GCTCTTCn'nnn_

Cuts at:      0     707    1455 
   Size:        707     748
 
SfcI C'TryA_G

Cuts at:      0      59     526     961    1384    1455 
   Size:         59     467     435     423      71

  Fragments arranged by size: 

                 467     435     423      71      59
 
SmlI C'TyrA_G

Cuts at:      0     269     472     727    1455 
   Size:        269     203     255     728

  Fragments arranged by size: 

                 728     269     255     203
 
SphI G_CATG'C

Cuts at:      0     631    1455 
   Size:        631     824
 
StyI C'CwwG_G

Cuts at:      0    1031    1455 
   Size:       1031     424
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     360     741     940    1455 
   Size:        360     381     199     515

  Fragments arranged by size: 

                 515     381     360     199
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     843     875    1455 
   Size:        843      32     580

  Fragments arranged by size: 

                 843     580      32
 
XbaI T'CTAG_A

Cuts at:      0     516    1455 
   Size:        516     939
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1139    1455 
   Size:       1139     316
 
XhoI C'TCGA_G

Cuts at:      0     472    1455 
   Size:        472     983
 
XmnI GAAnn'nnTTC

Cuts at:      0     391    1455 
   Size:        391    1064
 
ZraI GAC'GTC

Cuts at:      0    1434    1455 
   Size:       1434      21
 

 Enzymes that do cut:

    AatII     AccI   AflIII     AlfI    AlwNI     ApoI     AvaI    BamHI
     BanI    BanII Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BspMI   BstAPI    BstYI   Bsu36I     BtgI     BtsI     DrdI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII
    Hin4I   HincII     MscI     MslI   MspA1I     NspI     PasI     PciI
    PflMI     PfoI    PpuMI     PstI    PvuII     SacI     SapI     SfcI
     SmlI     SphI     StyI    TaqII     TstI     XbaI     XcmI     XhoI
     XmnI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI   Bpu10I    BsaBI    BsaWI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     EagI     EciI    EcoRV
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI     TatI
  Tth111I     XmaI
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