Restriction map for pF1KB4677
 (Linear) (Six-base) MAPSORT of: KIBB4677.seq  Check: 6264  from: 1  to: 2913



KIBB4677.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AatII G_ACGT'C

Cuts at:      0      81    2913 
   Size:         81    2832
 
AccI GT'mk_AC

Cuts at:      0    2436    2581    2806    2913 
   Size:       2436     145     225     107

  Fragments arranged by size: 

                2436     225     145     107
 
Acc65I G'GTAC_C

Cuts at:      0    2882    2913 
   Size:       2882      31
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170     950    1682    2053    2317    2872    2913 
   Size:        170     780     732     371     264     555      41

  Fragments arranged by size: 

                 780     732     555     371     264     170      41
 
AfeI AGC'GCT

Cuts at:      0    2864    2913 
   Size:       2864      49
 
AflIII A'CryG_T

Cuts at:      0    1850    2913 
   Size:       1850    1063
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2034    2913 
   Size:       2034     879
 
AlwNI CAG_nnn'CTG

Cuts at:      0    2208    2913 
   Size:       2208     705
 
ApaLI G'TGCA_C

Cuts at:      0     289    2913 
   Size:        289    2624
 
ApoI r'AATT_y

Cuts at:      0      91     248     317     487     685     853     894    1057 
   Size:         91     157      69     170     198     168      41     163

Cuts at:   1057    1426    1914    1972    2177    2337    2486    2774    2913 
   Size:        369     488      58     205     160     149     288     139

  Fragments arranged by size: 

                 488     369     288     205     198     170     168     163
                 160     157     149     139      91      69      58      41
            
 
AseI AT'TA_AT

Cuts at:      0     437    1523    2381    2913 
   Size:        437    1086     858     532

  Fragments arranged by size: 

                1086     858     532     437
 
BanI G'GyrC_C

Cuts at:      0    2882    2913 
   Size:       2882      31
 
BanII G_rGCy'C

Cuts at:      0     797    2913 
   Size:        797    2116
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2030    2913 
   Size:       2030     883
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1160    1194    2913 
   Size:       1160      34    1719

  Fragments arranged by size: 

                1719    1160      34
 
BglII A'GATC_T

Cuts at:      0    2519    2913 
   Size:       2519     394
 
BlpI GC'TnA_GC

Cuts at:      0     301    2913 
   Size:        301    2612
 
Bme1580I G_kGCm'C

Cuts at:      0     293    2409    2913 
   Size:        293    2116     504

  Fragments arranged by size: 

                2116     504     293
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     240    2851    2913 
   Size:        169      71    2611      62

  Fragments arranged by size: 

                2611     169      71      62
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     972    2913 
   Size:        972    1941
 
BsaBI GATnn'nnATC

Cuts at:      0      17      62    2913 
   Size:         17      45    2851

  Fragments arranged by size: 

                2851      45      17
 
BsaHI Gr'CG_yC

Cuts at:      0      78    2913 
   Size:         78    2835
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1915    1945    2913 
   Size:       1915      30     968

  Fragments arranged by size: 

                1915     968      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1114    1936    2913 
   Size:       1114     822     977

  Fragments arranged by size: 

                1114     977     822
 
BseYI C'CCAG_C

Cuts at:      0    2725    2913 
   Size:       2725     188
 
BsiEI CG_ry'CG

Cuts at:      0     264    2913 
   Size:        264    2649
 
BsiHKAI G_wGCw'C

Cuts at:      0     293     797    2270    2845    2913 
   Size:        293     504    1473     575      68

  Fragments arranged by size: 

                1473     575     504     293      68
 
BsmI GAATG_Cn'

Cuts at:      0    2128    2859    2913 
   Size:       2128     731      54

  Fragments arranged by size: 

                2128     731      54
 
Bsp1286I G_dGCh'C

Cuts at:      0     293     797    2270    2409    2845    2913 
   Size:        293     504    1473     139     436      68

  Fragments arranged by size: 

                1473     504     436     293     139      68
 
BspHI T'CATG_A

Cuts at:      0     483    1727    2913 
   Size:        483    1244    1186

  Fragments arranged by size: 

                1244    1186     483
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     875    2913 
   Size:        875    2038
 
BsrFI r'CCGG_y

Cuts at:      0     781    2913 
   Size:        781    2132
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     297     601     832    1570    2164    2913 
   Size:        297     304     231     738     594     749

  Fragments arranged by size: 

                 749     738     594     304     297     231
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     377    2081    2913 
   Size:        377    1704     832

  Fragments arranged by size: 

                1704     832     377
 
BstYI r'GATC_y

Cuts at:      0     675    1126    2519    2913 
   Size:        675     451    1393     394

  Fragments arranged by size: 

                1393     675     451     394
 
BstZ17I GTA'TAC

Cuts at:      0    2437    2582    2913 
   Size:       2437     145     331

  Fragments arranged by size: 

                2437     331     145
 
Bsu36I CC'TnA_GG

Cuts at:      0     613    2913 
   Size:        613    2300
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      26    2913 
   Size:         26    2887
 
BtsI GCAGTG_nn'

Cuts at:      0    2905    2913 
   Size:       2905       8
 
DraI TTT'AAA

Cuts at:      0      44     471    1406    2913 
   Size:         44     427     935    1507

  Fragments arranged by size: 

                1507     935     427      44
 
EaeI y'GGCC_r

Cuts at:      0     269    2913 
   Size:        269    2644
 
EarI CTCTTCn'nnn_

Cuts at:      0     767    1701    2913 
   Size:        767     934    1212

  Fragments arranged by size: 

                1212     934     767
 
EcoICRI GAG'CTC

Cuts at:      0     795    2913 
   Size:        795    2118
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     170     240     950    1682    2053    2317    2851 
   Size:        169       1      70     710     732     371     264     534

Cuts at:   2851    2872    2913 
   Size:         21      41

  Fragments arranged by size: 

                 732     710     534     371     264     169      70      41
                  21       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1202    2913 
   Size:       1202    1711
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1195    1600    2913 
   Size:       1195     405    1313

  Fragments arranged by size: 

                1313    1195     405
 
EcoRI G'AATT_C

Cuts at:      0     853    2337    2913 
   Size:        853    1484     576

  Fragments arranged by size: 

                1484     853     576
 
EcoRV GAT'ATC

Cuts at:      0    2695    2913 
   Size:       2695     218
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1513    1901    2902    2913 
   Size:       1513     388    1001      11

  Fragments arranged by size: 

                1513    1001     388      11
 
HaeII r_GCGC'y

Cuts at:      0    2866    2913 
   Size:       2866      47
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1393    1425    1512    1544    2020    2052    2913 
   Size:       1393      32      87      32     476      32     861

  Fragments arranged by size: 

                1393     861     476      87      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1266    2289    2913 
   Size:       1266    1023     624

  Fragments arranged by size: 

                1266    1023     624
 
HindIII A'AGCT_T

Cuts at:      0     520     751    2913 
   Size:        520     231    2162

  Fragments arranged by size: 

                2162     520     231
 
KpnI G_GTAC'C

Cuts at:      0    2886    2913 
   Size:       2886      27
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      99    2913 
   Size:         99    2814
 
MscI TGG'CCA

Cuts at:      0     271    2913 
   Size:        271    2642
 
MslI CAynn'nnrTG

Cuts at:      0     375     596    1280    2024    2913 
   Size:        375     221     684     744     889

  Fragments arranged by size: 

                 889     744     684     375     221
 
MspA1I CmG'CkG

Cuts at:      0      85    1627    2170    2913 
   Size:         85    1542     543     743

  Fragments arranged by size: 

                1542     743     543      85
 
NaeI GCC'GGC

Cuts at:      0     783    2913 
   Size:        783    2130
 
NgoMIV G'CCGG_C

Cuts at:      0     781    2913 
   Size:        781    2132
 
NspI r_CATG'y

Cuts at:      0    1854    2274    2913 
   Size:       1854     420     639

  Fragments arranged by size: 

                1854     639     420
 
PciI A'CATG_T

Cuts at:      0    1850    2913 
   Size:       1850    1063
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2591    2913 
   Size:       2591     322
 
PfoI T'CCnGG_A

Cuts at:      0    2817    2913 
   Size:       2817      96
 
PpuMI rG'GwC_Cy

Cuts at:      0    1195    2913 
   Size:       1195    1718
 
PsiI TTA'TAA

Cuts at:      0     989    2913 
   Size:        989    1924
 
PstI C_TGCA'G

Cuts at:      0     586    1241    1632    2169    2913 
   Size:        586     655     391     537     744

  Fragments arranged by size: 

                 744     655     586     537     391
 
PvuI CG_AT'CG

Cuts at:      0     264    2913 
   Size:        264    2649
 
PvuII CAG'CTG

Cuts at:      0      85    1627    2170    2913 
   Size:         85    1542     543     743

  Fragments arranged by size: 

                1542     743     543      85
 
SacI G_AGCT'C

Cuts at:      0     797    2913 
   Size:        797    2116
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1701    2913 
   Size:       1701    1212
 
SfcI C'TryA_G

Cuts at:      0     582     657    1237    1628    2165    2747    2913 
   Size:        582      75     580     391     537     582     166

  Fragments arranged by size: 

                 582     582     580     537     391     166      75
 
SpeI A'CTAG_T

Cuts at:      0     900    2913 
   Size:        900    2013
 
SspI AAT'ATT

Cuts at:      0      40    2100    2426    2913 
   Size:         40    2060     326     487

  Fragments arranged by size: 

                2060     487     326      40
 
StyI C'CwwG_G

Cuts at:      0    2235    2877    2913 
   Size:       2235     642      36

  Fragments arranged by size: 

                2235     642      36
 
TatI w'GTAC_w

Cuts at:      0    1103    2913 
   Size:       1103    1810
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     436    1265    2778    2913 
   Size:        436     829    1513     135

  Fragments arranged by size: 

                1513     829     436     135
 
XmnI GAAnn'nnTTC

Cuts at:      0     365    1737    1976    2913 
   Size:        365    1372     239     937

  Fragments arranged by size: 

                1372     937     365     239
 
ZraI GAC'GTC

Cuts at:      0      79    2913 
   Size:         79    2834
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AhdI    AlwNI
    ApaLI     ApoI     AseI     BanI    BanII     BbsI     BcgI    BglII
     BlpI Bme1580I     BpmI     BsaI    BsaBI    BsaHI    BsaXI    BseRI
    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrFI
   BstAPI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     DraI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI    HaeII    Hin4I   HincII  HindIII     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI   NgoMIV     NspI     PciI    PflMI     PfoI
    PpuMI     PsiI     PstI     PvuI    PvuII     SacI     SapI     SfcI
     SpeI     SspI     StyI     TatI     XcmI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI    AflII     AgeI     AleI     AlfI     AloI     ApaI
     AscI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI
    BciVI     BclI    BfrBI     BglI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaWI     BsgI    BsiWI    BsmBI    BspEI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII     BtgI
     ClaI    CspCI   DraIII     DrdI     EagI     EciI     FseI     FspI
    FspAI     HpaI     KasI     MfeI     MluI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI
     PpiI    PshAI   PspOMI    PspXI     PsrI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SphI     SrfI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XhoI     XmaI
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