################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BsaWI BseAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI KspI MaeI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 1024 GACGTC GACGT^C AccI 904 GTCTAC GT^[AC][GT]AC AflIII 884 ACACGT A^C[GA][CT]GT AocI 270 CCTCAGG CC^T[ACGT]AGG Asp700I 561 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 635 GGATCC G^GATCC BanI 118 GGCACC G^G[CT][GA]CC BglII 956 AGATCT A^GATCT BlpI 681 GCTCAGC GC^T[ACGT]AGC Bpu1102I 681 GCTCAGC GC^T[ACGT]AGC BsaAI 885 CACGTA [CT]AC^GT[GA] BsaBI 156 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 946 GAGACC GGTCTC(1/5) BseRI 892 GAGGAG GAGGAG(10/8) BsgI 313 GTGCAG GTGCAG(16/14) BsiEI 294 CGACCG CG[GA][CT]^CG BsiWI 887 CGTACG C^GTACG BsmAI 946 GAGAC GTCTC(1/5) Bsu36I 270 CCTCAGG CC^T[ACGT]AGG CelII 681 GCTCAGC GC^T[ACGT]AGC DsaI 545 CCACGG C^C[GA][CT]GG EaeI 263 TGGCCG [CT]^GGCC[GA] EarI 799 CTCTTC CTCTTC(1/4) Eco81I 270 CCTCAGG CC^T[ACGT]AGG EcoT14I 950 CCAAGG C^C[AT][AT]GG Ksp632I 799 CTCTTC CTCTTC(1/4) MamI 156 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC PpuMI 815 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1020 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 494 CAGCTG CAG^CTG SmaI 533 CCCGGG CCC^GGG SmlI 1045 CTCAAG C^T[CT][GA]AG StyI 950 CCAAGG C^C[AT][AT]GG Tsp509I 54 AATT ^AATT XcmI 545 CCACGGGCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 533 CCCGGG C^CCGGG XmaI 533 CCCGGG C^CCGGG XmnI 561 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcyI 652 GGCGTC G[GA]^CG[CT]C 1024 GACGTC ApaI 257 GGGCCC GGGCC^C 333 GGGCCC AvaII 44 GGTCC G^G[AT]CC 816 GGACC BbsI 925 GAAGAC GAAGAC(2/6) 1006 GTCTTC BpuAI 925 GAAGAC GAAGAC(2/6) 1006 GTCTTC BsaHI 652 GGCGTC G[GA]^CG[CT]C 1024 GACGTC BsmFI 536 GGGAC GGGAC(10/14) 45 GTCCC BsrBI 426 CCGCTC CCGCTC(-3/-3) 847 CCGCTC HgaI 1012 GACGC GACGC(5/10) 653 GCGTC Hin1I 652 GGCGTC G[GA]^CG[CT]C 1024 GACGTC HinfI 235 GACTC G^A[ACGT]TC 754 GACTC NlaIII 381 CATG CATG^ 540 CATG PleI 235 GACTC GAGTC(4/5) 754 GACTC SacI 580 GAGCTC GAGCT^C 679 GAGCTC StuI 191 AGGCCT AGG^CCT 413 AGGCCT Tsp45I 655 GTCAC ^GT[GC]AC 1018 GTGAC TspRI 510 CACTG CA[GC]TG[ACGT][ACGT]^ 640 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaI BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI TthHB8I XhoII