(Linear) (Six-base) MAPSORT of: KIBB4667.seq Check: 3385 from: 1 to: 1065 KIBB4667.orf With 182 enzymes: SgfI * March 3, 2008 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 74 982 1065 Size: 74 908 83 Fragments arranged by size: 908 83 74 AatII G_ACGT'C Cuts at: 0 1028 1065 Size: 1028 37 AccI GT'mk_AC Cuts at: 0 905 1065 Size: 905 160 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 405 1065 Size: 405 660 AflIII A'CryG_T Cuts at: 0 884 1065 Size: 884 181 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 338 432 1065 Size: 338 94 633 Fragments arranged by size: 633 338 94 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 550 582 1065 Size: 550 32 483 Fragments arranged by size: 550 483 32 ApaI G_GGCC'C Cuts at: 0 261 337 1065 Size: 261 76 728 Fragments arranged by size: 728 261 76 AvaI C'yCGr_G Cuts at: 0 364 533 865 1065 Size: 364 169 332 200 Fragments arranged by size: 364 332 200 169 BamHI G'GATC_C Cuts at: 0 635 1065 Size: 635 430 BanI G'GyrC_C Cuts at: 0 118 1065 Size: 118 947 BanII G_rGCy'C Cuts at: 0 261 337 584 683 1065 Size: 261 76 247 99 382 Fragments arranged by size: 382 261 247 99 76 BbsI GAAGACnn'nnnn_ Cuts at: 0 932 999 1065 Size: 932 67 66 Fragments arranged by size: 932 67 66 BglII A'GATC_T Cuts at: 0 956 1065 Size: 956 109 BlpI GC'TnA_GC Cuts at: 0 682 1065 Size: 682 383 Bme1580I G_kGCm'C Cuts at: 0 121 261 337 411 1065 Size: 121 140 76 74 654 Fragments arranged by size: 654 140 121 76 74 BmgBI CAC'GTC Cuts at: 0 603 1065 Size: 603 462 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 978 1065 Size: 978 87 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 414 466 499 1065 Size: 208 206 52 33 566 Fragments arranged by size: 566 208 206 52 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1030 1065 Size: 1030 35 BsaI GGTCTCn'nnnn_ Cuts at: 0 940 1065 Size: 940 125 BsaAI yAC'GTr Cuts at: 0 887 1065 Size: 887 178 BsaBI GATnn'nnATC Cuts at: 0 160 1065 Size: 160 905 BsaHI Gr'CG_yC Cuts at: 0 653 1025 1065 Size: 653 372 40 Fragments arranged by size: 653 372 40 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 550 580 1065 Size: 550 30 485 Fragments arranged by size: 550 485 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 907 1065 Size: 907 158 BseYI C'CCAG_C Cuts at: 0 114 372 1065 Size: 114 258 693 Fragments arranged by size: 693 258 114 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 1065 Size: 334 731 BsiEI CG_ry'CG Cuts at: 0 297 1065 Size: 297 768 BsiHKAI G_wGCw'C Cuts at: 0 142 584 683 1065 Size: 142 442 99 382 Fragments arranged by size: 442 382 142 99 BsiWI C'GTAC_G Cuts at: 0 887 1065 Size: 887 178 Bsp1286I G_dGCh'C Cuts at: 0 121 142 261 337 411 584 683 1065 Size: 121 21 119 76 74 173 99 382 Fragments arranged by size: 382 173 121 119 99 76 74 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 74 827 982 1065 Size: 74 753 155 83 Fragments arranged by size: 753 155 83 74 BsrBI CCG'CTC Cuts at: 0 428 849 1065 Size: 428 421 216 Fragments arranged by size: 428 421 216 BstYI r'GATC_y Cuts at: 0 635 833 956 1065 Size: 635 198 123 109 Fragments arranged by size: 635 198 123 109 Bsu36I CC'TnA_GG Cuts at: 0 271 1065 Size: 271 794 BtgI C'CryG_G Cuts at: 0 545 1065 Size: 545 520 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 471 1065 Size: 471 594 BtsI GCAGTG_nn' Cuts at: 0 639 1065 Size: 639 426 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 534 569 1065 Size: 534 35 496 Fragments arranged by size: 534 496 35 EaeI y'GGCC_r Cuts at: 0 263 1065 Size: 263 802 EarI CTCTTCn'nnn_ Cuts at: 0 805 1065 Size: 805 260 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 826 1065 Size: 826 239 EcoICRI GAG'CTC Cuts at: 0 582 681 1065 Size: 582 99 384 Fragments arranged by size: 582 384 99 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 405 414 466 499 1065 Size: 208 197 9 52 33 566 Fragments arranged by size: 566 208 197 52 33 9 EcoO109I rG'GnC_Cy Cuts at: 0 258 334 816 1065 Size: 258 76 482 249 Fragments arranged by size: 482 258 249 76 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 588 1065 Size: 588 477 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 706 1065 Size: 706 359 MspA1I CmG'CkG Cuts at: 0 84 173 340 496 686 901 915 1065 Size: 84 89 167 156 190 215 14 150 Fragments arranged by size: 215 190 167 156 150 89 84 14 PasI CC'CwG_GG Cuts at: 0 403 1065 Size: 403 662 PfoI T'CCnGG_A Cuts at: 0 46 1065 Size: 46 1019 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 550 582 1065 Size: 550 32 483 Fragments arranged by size: 550 483 32 PpuMI rG'GwC_Cy Cuts at: 0 816 1065 Size: 816 249 PshAI GACnn'nnGTC Cuts at: 0 1024 1065 Size: 1024 41 PspOMI G'GGCC_C Cuts at: 0 257 333 1065 Size: 257 76 732 Fragments arranged by size: 732 257 76 PvuII CAG'CTG Cuts at: 0 496 1065 Size: 496 569 SacI G_AGCT'C Cuts at: 0 584 683 1065 Size: 584 99 382 Fragments arranged by size: 584 382 99 SmaI CCC'GGG Cuts at: 0 535 1065 Size: 535 530 SmlI C'TyrA_G Cuts at: 0 1045 1065 Size: 1045 20 StuI AGG'CCT Cuts at: 0 193 415 1065 Size: 193 222 650 Fragments arranged by size: 650 222 193 StyI C'CwwG_G Cuts at: 0 950 1065 Size: 950 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 364 1065 Size: 364 701 TatI w'GTAC_w Cuts at: 0 203 869 1049 1065 Size: 203 666 180 16 Fragments arranged by size: 666 203 180 16 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 552 1065 Size: 552 513 XmaI C'CCGG_G Cuts at: 0 533 1065 Size: 533 532 XmnI GAAnn'nnTTC Cuts at: 0 565 1065 Size: 565 500 ZraI GAC'GTC Cuts at: 0 1026 1065 Size: 1026 39 Enzymes that do cut: AarI AatII AccI AcuI AflIII AlfI AloI ApaI AvaI BamHI BanI BanII BbsI BglII BlpI Bme1580I BmgBI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspMI BsrBI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI MmeI MspA1I PasI PfoI PpiI PpuMI PshAI PspOMI PvuII SacI SmaI SmlI StuI StyI TaqII TatI XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI AleI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BmrI BmtI Bpu10I BsaWI BsmI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I ClaI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI