Restriction map for pF1KB4666
##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AciI        
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BciVI       BclI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EarI        EclXI       
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FseI        FspI        HaeIII      
HgaI        Hin1I       HincII      HindII      HindIII     
HinfI       HpaI        HphI        KasI        KpnI        
Ksp632I     KspI        MaeII       MamI        MboII       
MfeI        MluI        MluNI       MroI        MscI        
MseI        MslI        MunI        MvaI        MwoI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TaqI        TfiI        
Tru9I       Tsp45I      Tsp509I     TspRI       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AccII          18  CGCG                 CG^CG
AfaI          111  GTAC                 GT^AC
Aor51HI        32  AGCGCT               AGC^GCT
BcgI           20  CGAGCGCCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI           81  GGTCTC               GGTCTC(1/5)
BsaJI           8  CCGGGG               C^C[ACGT][ACGT]GG
BsmAI          82  GTCTC                GTCTC(1/5)
BsmI          123  GAATGC               GAATGC(1/-1)
BstF5I        135  GGATG                GGATG(2/0)
BstUI          18  CGCG                 CG^CG
BstYI         187  AGATCC               [GA]^GATC[CT]
DdeI           86  CTCAG                C^T[ACGT]AG
Eco47III       32  AGCGCT               AGC^GCT
FokI          135  GGATG                GGATG(9/13)
MaeIII        167  GTAAC                ^GT[ACGT]AC
MflI          187  AGATCC               [GA]^GATC[CT]
MvnI           18  CGCG                 CG^CG
NheI           13  GCTAGC               G^CTAGC
NlaIV         180  GGAGCC               GG[ACGT]^[ACGT]CC
RsaI          111  GTAC                 GT^AC
ScaI          110  AGTACT               AGT^ACT
SfaNI         136  GATGC                GCATC(5/9)
SmlI           49  CTCAAG               C^T[CT][GA]AG
XhoII         187  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AlwI          188  GATCC                GGATC(4/5)
               74  GGATC               
BcnI            8  CCGGG                CC^[GC]GG
              177  CCGGG               
BfaI           14  CTAG                 C^TAG
              185  CTAG                
BsiYI         141  CCTGCTGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              171  CCCCTTCCGGG         
BslI          141  CCTGCTGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              171  CCCCTTCCGGG         
BssKI           8  CCGGG                ^CC[ACGT]GG
              177  CCGGG               
DpnII          75  GATC                 ^GATC
              188  GATC                
HaeII          22  AGCGCC               [GA]GCGC^[CT]
               32  AGCGCT              
HapII           8  CCGG                 C^CGG
              177  CCGG                
HpaII           8  CCGG                 C^CGG
              177  CCGG                
MaeI           14  CTAG                 C^TAG
              185  CTAG                
MboI           75  GATC                 ^GATC
              188  GATC                
MspA1I         92  CAGCTG               C[AC]G^C[GT]G
              158  CAGCTG              
MspI            8  CCGG                 C^CGG
              177  CCGG                
NciI            8  CCGGG                CC^[GC]GG
              177  CCGGG               
NdeII          75  GATC                 ^GATC
              188  GATC                
PstI           28  CTGCAG               CTGCA^G
               95  CTGCAG              
PvuII          92  CAGCTG               CAG^CTG
              158  CAGCTG              
Sau3AI         75  GATC                 ^GATC
              188  GATC                
ScrFI           8  CCGGG                CC^[ACGT]GG
              177  CCGGG               
SfcI           28  CTGCAG               C^T[GA][CT]AG
               95  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AluI        BbvI        Cac8I       CfoI        Fnu4HI      
HhaI        HinP1I      ItaI        MnlI        TseI        
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