Restriction map for pF1KB4600
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcsI        AcyI        AflII       AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BamHI       BanI        BanII       BbeI        
BbsI        BciVI       BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmI        BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        HphI        KasI        KpnI        
KspI        MaeI        MamI        MfeI        MluI        
MroI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PshAI       
PshBI       PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfaNI       SfiI        SfoI        SgrAI       
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        StyI        SwaI        Tsp45I      
TspRI       Tth111I     Van91I      XbaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AclI           10  AACGTT               AA^CGTT
AflIII        423  ACGTGT               A^C[GA][CT]GT
Asp700I       488  GAAACACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         792  GGACC                G^G[AT]CC
BbrPI         422  CACGTG               CAC^GTG
BcgI          103  GCAATCTTTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          845  TGATCA               T^GATCA
BmyI          460  GTGCCC               G[AGT]GC[ACT]^C
BpmI          838  CTGGAG               CTGGAG(16/14)
BsaAI         422  CACGTG               [CT]AC^GT[GA]
BsgI          767  GTGCAG               GTGCAG(16/14)
Bsp1286I      460  GTGCCC               G[AGT]GC[ACT]^C
Bsp1407I      407  TGTACA               T^GTACA
BspMI         283  GCAGGT               ACCTGC(4/8)
BsrGI         407  TGTACA               T^GTACA
BsrI          798  ACTGG                ACTGG(1/-1)
BstBI         111  TTCGAA               TT^CGAA
CfoI          896  GCGC                 GCG^C
Cfr13I        792  GGACC                G^G[ACGT]CC
DraII         791  GGGACCT              [GA]G^G[ACGT]CC[CT]
EarI          700  GAAGAG               CTCTTC(1/4)
EcoO109I      791  GGGACCT              [GA]G^G[ACGT]CC[CT]
FauI          537  GCGGG                CCCGC(4/6)
FbaI          845  TGATCA               T^GATCA
HaeII         895  AGCGCC               [GA]GCGC^[CT]
HapII         189  CCGG                 C^CGG
HhaI          896  GCGC                 GCG^C
HinP1I        896  GCGC                 G^CGC
HpaII         189  CCGG                 C^CGG
Ksp632I       700  GAAGAG               CTCTTC(1/4)
MslI           72  CACTCTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          189  CCGG                 C^CGG
NlaIII         39  CATG                 CATG^
NlaIV         791  GGGACC               GG[ACGT]^[ACGT]CC
NspV          111  TTCGAA               TT^CGAA
PmaCI         422  CACGTG               CAC^GTG
PmlI          422  CACGTG               CAC^GTG
PpuMI         791  GGGACCT              [GA]G^G[AT]CC[CT]
Psp1406I       10  AACGTT               AA^CGTT
PvuII         835  CAGCTG               CAG^CTG
Sau96I        792  GGACC                G^G[ACGT]CC
SfuI          111  TTCGAA               TT^CGAA
SmaI          188  CCCGGG               CCC^GGG
SspBI         407  TGTACA               T^GTACA
XmaCI         188  CCCGGG               C^CCGGG
XmaI          188  CCCGGG               C^CCGGG
XmnI          488  GAAACACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AciI          304  GCGG                 CCGC(-3/-1)
              537  GCGG                
AlwI           28  GATCC                GGATC(4/5)
              541  GGATC               
AvaI          188  CCCGGG               C^[CT]CG[GA]G
              449  CTCGGG              
BalI            2  TGGCCA               TGG^CCA
              228  TGGCCA              
BbvCI         781  GCTGAGG              CCTCAGC(-5/-2)
              868  GCTGAGG             
BcnI          188  CCCGG                CC^[GC]GG
              189  CCGGG               
BsaJI         188  CCCGGG               C^C[ACGT][ACGT]GG
              273  CCGAGG              
BsmAI         736  GAGAC                GTCTC(1/5)
              890  GAGAC               
BsoBI         188  CCCGGG               C^[CT]CG[GA]G
              449  CTCGGG              
BstYI          27  AGATCC               [GA]^GATC[CT]
              541  GGATCT              
Cac8I           4  GCCAGC               GC[ACGT]^[ACGT]GC
              226  GCTGGC              
EaeI            2  TGGCCA               [CT]^GGCC[GA]
              228  TGGCCA              
MflI           27  AGATCC               [GA]^GATC[CT]
              541  GGATCT              
MluNI           2  TGGCCA               TGG^CCA
              228  TGGCCA              
MscI            2  TGGCCA               TGG^CCA
              228  TGGCCA              
NciI          188  CCCGG                CC^[GC]GG
              189  CCGGG               
PstI          254  CTGCAG               CTGCA^G
              299  CTGCAG              
TaqI          112  TCGA                 T^CGA
              466  TCGA                
TfiI           63  GAATC                G^A[AT]TC
              622  GATTC               
TthHB8I       112  TCGA                 T^CGA
              466  TCGA                
XhoII          27  AGATCC               [GA]^GATC[CT]
              541  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   34 enzymes) #
#########################################

AfaI        AluI        BbvI        BseRI       BsmFI       
BssKI       BstF5I      BstNI       DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspA1I      MvaI        MwoI        
NdeII       PleI        RsaI        Sau3AI      ScrFI       
SfcI        Tru9I       TseI        Tsp509I     
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