Restriction map for pF1KB4600
 (Circular) (Six-base) MAPSORT of: pF1KB4600.seq  Check: 3873  from: 1  to: 4031



pF1KB4600  4031 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    382     382 
   Size:       4031
 
AccI GT'mk_AC

Cuts at:   1069    1069 
   Size:       4031
 
Acc65I G'GTAC_C

Cuts at:   1047    1047 
   Size:       4031
 
AclI AA'CG_TT

Cuts at:    119    3871     119 
   Size:       3752     279
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    670    1856    2288    3035    3303     670 
   Size:       1186     432     747     268    1398

  Fragments arranged by size: 

                1398    1186     747     432     268
 
AflIII A'CryG_T

Cuts at:    531    1383    2487    3464     531 
   Size:        852    1104     977    1098

  Fragments arranged by size: 

                1104    1098     977     852
 
AgeI A'CCGG_T

Cuts at:   2419    2419 
   Size:       4031
 
AleI CACnn'nnGTG

Cuts at:    184     184 
   Size:       4031
 
AlwNI CAG_nnn'CTG

Cuts at:   1336    2903    1336 
   Size:       1567    2464
 
ApaLI G'TGCA_C

Cuts at:   2801    2801 
   Size:       4031
 
ApoI r'AATT_y

Cuts at:   1035    3384    3640    1035 
   Size:       2349     256    1426

  Fragments arranged by size: 

                2349    1426     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4031
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4031
 
AvaI C'yCGr_G

Cuts at:    296     557    1051     296 
   Size:        261     494    3276

  Fragments arranged by size: 

                3276     494     261
 
BamHI G'GATC_C

Cuts at:   1056    1056 
   Size:       4031
 
BanI G'GyrC_C

Cuts at:   1047    1461    1709    1744    1047 
   Size:        414     248      35    3334

  Fragments arranged by size: 

                3334     414     248      35
 
BanII G_rGCy'C

Cuts at:   1045    2075    1045 
   Size:       1030    3001
 
BbeI G_GCGC'C

Cuts at:   1465    1713    1465 
   Size:        248    3783
 
BbvCI CC'TCA_GC

Cuts at:    890     977     890 
   Size:         87    3944
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    200     234     200 
   Size:         34    3997
 
BciVI GTATCCnnnnn_n'

Cuts at:   1929    2690    4031    1929 
   Size:        761    1341    1929

  Fragments arranged by size: 

                1929    1341     761
 
BclI T'GATC_A

Cuts at:    953    1551     953 
   Size:        598    3433
 
BglII A'GATC_T

Cuts at:   1546    3583    1546 
   Size:       2037    1994
 
BlpI GC'TnA_GC

Cuts at:   1143    1143 
   Size:       4031
 
Bme1580I G_kGCm'C

Cuts at:    572    1656    1749    2805     572 
   Size:       1084      93    1056    1798

  Fragments arranged by size: 

                1798    1084    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1243    1324    1659    3803    1243 
   Size:         81     335    2144    1471

  Fragments arranged by size: 

                2144    1471     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1038    2398    1038 
   Size:       1360    2671
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    967     967 
   Size:       4031
 
Bpu10I CC'TnA_GC

Cuts at:    890     977     890 
   Size:         87    3944
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1207    2086    2578    2876    1207 
   Size:        879     492     298    2362

  Fragments arranged by size: 

                2362     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3735    3735 
   Size:       4031
 
BsaAI yAC'GTr

Cuts at:    532    1384     532 
   Size:        852    3179
 
BsaBI GATnn'nnATC

Cuts at:      6    1545    3582       6 
   Size:       1539    2037     455

  Fragments arranged by size: 

                2037    1539     455
 
BsaHI Gr'CG_yC

Cuts at:   1462    1710    2412    3908    1462 
   Size:        248     702    1496    1585

  Fragments arranged by size: 

                1585    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1220    1741    2419    2693    2840    3251    1220 
   Size:        521     678     274     147     411    2000

  Fragments arranged by size: 

                2000     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    456     530     533     536     826     907     456 
   Size:         74       3       3     290      81    3580

  Fragments arranged by size: 

                3580     290      81      74       3       3
 
BseYI C'CCAG_C

Cuts at:    525    1456    2214    2791    3287     525 
   Size:        931     758     577     496    1269

  Fragments arranged by size: 

                1269     931     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    896     896 
   Size:       4031
 
BsiEI CG_ry'CG

Cuts at:    104    1573    1619    2827     104 
   Size:       1469      46    1208    1308

  Fragments arranged by size: 

                1469    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   1045    1823    2013    2805    1045 
   Size:        778     190     792    2271

  Fragments arranged by size: 

                2271     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    572    1045    1656    1749    1823    2013    2075    2805     572 
   Size:        473     611      93      74     190      62     730    1798

  Fragments arranged by size: 

                1798     730     611     473     190      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   3251    3251 
   Size:       4031
 
BspHI T'CATG_A

Cuts at:   3207    3207 
   Size:       4031
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    382    1081    1295    1597    1978     382 
   Size:        699     214     302     381    2435

  Fragments arranged by size: 

                2435     699     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2323    2377    3861    4026      50 
   Size:       2273      54    1484     165      55

  Fragments arranged by size: 

                2273    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1943    1943 
   Size:       4031
 
BsrFI r'CCGG_y

Cuts at:   2029    2210    2419    2029 
   Size:        181     209    3641

  Fragments arranged by size: 

                3641     209     181
 
BsrGI T'GTAC_A

Cuts at:    515     515 
   Size:       4031
 
BssSI C'ACGA_G

Cuts at:   2302    2660    2302 
   Size:        358    3673
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1408    1408 
   Size:       4031
 
BstBI TT'CG_AA

Cuts at:    220    2392     220 
   Size:       2172    1859
 
BstYI r'GATC_y

Cuts at:      1     135     649    1056    1523    1546    1881    2127    3139 
   Size:        134     514     407     467      23     335     246    1012

Cuts at:   3139    3225    3237    3583       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     514     467     449     407     346     335     246
                 134      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4031
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1981    2162    2325    1981 
   Size:        181     163    3687

  Fragments arranged by size: 

                3687     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1274    1274 
   Size:       4031
 
DraI TTT'AAA

Cuts at:   1030    1030 
   Size:       4031
 
DrdI GACnn_nn'nnGTC

Cuts at:   1737    2595    1737 
   Size:        858    3173
 
EaeI y'GGCC_r

Cuts at:    110     336    1616    1790    2181    2208    3553     110 
   Size:        226    1280     174     391      27    1345     588

  Fragments arranged by size: 

                1345    1280     588     391     226     174      27
 
EagI C'GGCC_G

Cuts at:   1616    1616 
   Size:       4031
 
EarI CTCTTCn'nnn_

Cuts at:    803    2054    2264     803 
   Size:       1251     210    2570

  Fragments arranged by size: 

                2570    1251     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2239    2549    2695    3841    2239 
   Size:        310     146    1146    2429

  Fragments arranged by size: 

                2429    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1043    1043 
   Size:       4031
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    670     967    1856    2288    3035    3303     670 
   Size:        297     889     432     747     268    1398

  Fragments arranged by size: 

                1398     889     747     432     297     268
 
EcoO109I rG'GnC_Cy

Cuts at:    900    1170     900 
   Size:        270    3761
 
EcoRI G'AATT_C

Cuts at:   1035    1035 
   Size:       4031
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2050    2050 
   Size:       4031
 
FspI TGC'GCA

Cuts at:   1812    1812 
   Size:       4031
 
HaeII r_GCGC'y

Cuts at:   1007    1465    1713    2735    3714    1007 
   Size:        458     248    1022     979    1324

  Fragments arranged by size: 

                1324    1022     979     458     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    123     127     155     159    1873    1903    1905    1935     123 
   Size:          4      28       4    1714      30       2      30    2219

  Fragments arranged by size: 

                2219    1714      30      30      28       4       4       2
            
 
HincII GTy'rAC

Cuts at:   1070    1070 
   Size:       4031
 
KasI G'GCGC_C

Cuts at:   1461    1709    1461 
   Size:        248    3783
 
KpnI G_GTAC'C

Cuts at:   1051    1051 
   Size:       4031
 
MluI A'CGCG_T

Cuts at:   3464    3464 
   Size:       4031
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2702    2886    2702 
   Size:        184    3847
 
MscI TGG'CCA

Cuts at:    112     338    1792     112 
   Size:        226    1454    2351

  Fragments arranged by size: 

                2351    1454     226
 
MslI CAynn'nnrTG

Cuts at:    184    2020    2147     184 
   Size:       1836     127    2068

  Fragments arranged by size: 

                2068    1836     127
 
MspA1I CmG'CkG

Cuts at:    412     645     945    1142    1456    1816    2829    3074     412 
   Size:        233     300     197     314     360    1013     245    1369

  Fragments arranged by size: 

                1369    1013     360     314     300     245     233     197
            
 
NaeI GCC'GGC

Cuts at:   2212    2212 
   Size:       4031
 
NarI GG'CG_CC

Cuts at:   1462    1710    1462 
   Size:        248    3783
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4031
 
NgoMIV G'CCGG_C

Cuts at:   2210    2210 
   Size:       4031
 
NspI r_CATG'y

Cuts at:   1084    1584    2491    1084 
   Size:        500     907    2624

  Fragments arranged by size: 

                2624     907     500
 
PciI A'CATG_T

Cuts at:   2487    2487 
   Size:       4031
 
PfoI T'CCnGG_A

Cuts at:    737     737 
   Size:       4031
 
PmeI GTTT'AAAC

Cuts at:   1030    1030 
   Size:       4031
 
PmlI CAC'GTG

Cuts at:    532     532 
   Size:       4031
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3170    3202    3170 
   Size:         32    3999
 
PpuMI rG'GwC_Cy

Cuts at:    900     900 
   Size:       4031
 
PstI C_TGCA'G

Cuts at:    366     411    1078    1763     366 
   Size:         45     667     685    2634

  Fragments arranged by size: 

                2634     685     667      45
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4031
 
PvuII CAG'CTG

Cuts at:    945    1456    1816     945 
   Size:        511     360    3160

  Fragments arranged by size: 

                3160     511     360
 
RsrII CG'GwC_CG

Cuts at:   2226    2226 
   Size:       4031
 
SacI G_AGCT'C

Cuts at:   1045    1045 
   Size:       4031
 
SalI G'TCGA_C

Cuts at:   1068    1068 
   Size:       4031
 
SapI GCTCTTCn'nnn_

Cuts at:   2054    2264    2054 
   Size:        210    3821
 
SbfI CC_TGCA'GG

Cuts at:   1078    1078 
   Size:       4031
 
ScaI AGT'ACT

Cuts at:   3548    3548 
   Size:       4031
 
SfcI C'TryA_G

Cuts at:     33     305     362     407     563    1074    1759    2752    2943 
   Size:        272      57      45     156     511     685     993     191

Cuts at:   2943      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     685     511     272     191     156      57
                  45
 
SfoI GGC'GCC

Cuts at:   1463    1711    1463 
   Size:        248    3783
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4031
 
SmaI CCC'GGG

Cuts at:    298    1053     298 
   Size:        755    3276
 
SmlI C'TyrA_G

Cuts at:   1186    2101    2593    2855    1186 
   Size:        915     492     262    2362

  Fragments arranged by size: 

                2362     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4031
 
SphI G_CATG'C

Cuts at:   1084    1584    1084 
   Size:        500    3531
 
SrfI GCCC'GGGC

Cuts at:    298     298 
   Size:       4031
 
StyI C'CwwG_G

Cuts at:    107    1165    1519     107 
   Size:       1058     354    2619

  Fragments arranged by size: 

                2619    1058     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1614    1747    2206    2415    1614 
   Size:        133     459     209    3230

  Fragments arranged by size: 

                3230     459     209     133
 
TatI w'GTAC_w

Cuts at:    515    3546     515 
   Size:       3031    1000
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3548    3580    3548 
   Size:         32    3999
 
Tth111I GACn'n_nGTC

Cuts at:   1828    1828 
   Size:       4031
 
XbaI T'CTAG_A

Cuts at:   1062    1062 
   Size:       4031
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1526    1526 
   Size:       4031
 
XmaI C'CCGG_G

Cuts at:    296    1051     296 
   Size:        755    3276
 
XmnI GAAnn'nnTTC

Cuts at:    600     600 
   Size:       4031
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     SrfI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI    BfrBI     BglI    BmgBI     BmtI    BsaXI
    BsiWI     BsmI    BsmBI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I
     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SspI     StuI     SwaI
     XhoI     ZraI
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