Restriction map for
pF1KB4426
(Circular) (Six-base) MAPSORT of: pF1KB4426.seq Check: 7164 from: 1 to: 3929
pF1KB4426 3929 bp
With 182 enzymes: SgfI *
June 20, 2008 10:24 ..
AccI GT'mk_AC
Cuts at: 314 967 314
Size: 653 3276
Acc65I G'GTAC_C
Cuts at: 945 945
Size: 3929
AclI AA'CG_TT
Cuts at: 917 3769 917
Size: 2852 1077
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 575 1754 2186 2933 3201 568
Size: 7 1179 432 747 268 1296
Fragments arranged by size:
1296 1179 747 432 268 7
AfeI AGC'GCT
Cuts at: 325 325
Size: 3929
AflIII A'CryG_T
Cuts at: 1281 2385 3362 1281
Size: 1104 977 1848
Fragments arranged by size:
1848 1104 977
AgeI A'CCGG_T
Cuts at: 2317 2317
Size: 3929
AlwNI CAG_nnn'CTG
Cuts at: 168 339 1234 2801 168
Size: 171 895 1567 1296
Fragments arranged by size:
1567 1296 895 171
ApaI G_GGCC'C
Cuts at: 679 679
Size: 3929
ApaLI G'TGCA_C
Cuts at: 181 2699 181
Size: 2518 1411
ApoI r'AATT_y
Cuts at: 933 3282 3538 933
Size: 2349 256 1324
Fragments arranged by size:
2349 1324 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3929
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
AvaI C'yCGr_G
Cuts at: 949 949
Size: 3929
BamHI G'GATC_C
Cuts at: 954 954
Size: 3929
BanI G'GyrC_C
Cuts at: 735 945 1359 1607 1642 735
Size: 210 414 248 35 3022
Fragments arranged by size:
3022 414 248 210 35
BanII G_rGCy'C
Cuts at: 175 194 473 679 716 943 1973 175
Size: 19 279 206 37 227 1030 2131
Fragments arranged by size:
2131 1030 279 227 206 37 19
BbeI G_GCGC'C
Cuts at: 1363 1611 1363
Size: 248 3681
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 604 638 604
Size: 34 3895
BciVI GTATCCnnnnn_n'
Cuts at: 1827 2588 3929 1827
Size: 761 1341 1827
Fragments arranged by size:
1827 1341 761
BclI T'GATC_A
Cuts at: 1449 1449
Size: 3929
BglI GCCn_nnn'nGGC
Cuts at: 236 236
Size: 3929
BglII A'GATC_T
Cuts at: 512 1444 3481 512
Size: 932 2037 960
Fragments arranged by size:
2037 960 932
BlpI GC'TnA_GC
Cuts at: 1041 1041
Size: 3929
Bme1580I G_kGCm'C
Cuts at: 185 223 679 740 1554 1647 2703 185
Size: 38 456 61 814 93 1056 1411
Fragments arranged by size:
1411 1056 814 456 93 61 38
BmrI ACTGGGnnnn_n'
Cuts at: 585 1141 1222 1557 3701 585
Size: 556 81 335 2144 813
Fragments arranged by size:
2144 813 556 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 468 519 2296 468
Size: 51 1777 2101
Fragments arranged by size:
2101 1777 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1105 1984 2476 2774 1105
Size: 879 492 298 2260
Fragments arranged by size:
2260 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 490 3633 490
Size: 3143 786
BsaAI yAC'GTr
Cuts at: 1282 1282
Size: 3929
BsaBI GATnn'nnATC
Cuts at: 6 1443 3480 6
Size: 1437 2037 455
Fragments arranged by size:
2037 1437 455
BsaHI Gr'CG_yC
Cuts at: 311 1360 1608 2310 3806 311
Size: 1049 248 702 1496 434
Fragments arranged by size:
1496 1049 702 434 248
BsaWI w'CCGG_w
Cuts at: 1118 1639 2317 2591 2738 3149 1118
Size: 521 678 274 147 411 1898
Fragments arranged by size:
1898 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 379 481 508 379
Size: 102 27 3800
Fragments arranged by size:
3800 102 27
BseYI C'CCAG_C
Cuts at: 226 1354 2112 2689 3185 226
Size: 1128 758 577 496 970
Fragments arranged by size:
1128 970 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 361 373 629 361
Size: 12 256 3661
Fragments arranged by size:
3661 256 12
BsiEI CG_ry'CG
Cuts at: 104 396 1471 1517 2725 104
Size: 292 1075 46 1208 1308
Fragments arranged by size:
1308 1208 1075 292 46
BsiHKAI G_wGCw'C
Cuts at: 185 464 716 943 1721 1911 2703 185
Size: 279 252 227 778 190 792 1411
Fragments arranged by size:
1411 792 778 279 252 227 190
Bsp1286I G_dGCh'C
Cuts at: 175 185 194 223 464 473 679 716 740
Size: 10 9 29 241 9 206 37 24
Cuts at: 740 943 1554 1647 1721 1911 1973 2703 175
Size: 203 611 93 74 190 62 730 1401
Fragments arranged by size:
1401 730 611 241 206 203 190 93
74 62 37 29 24 10 9 9
BspEI T'CCGG_A
Cuts at: 3149 3149
Size: 3929
BspHI T'CATG_A
Cuts at: 3105 3105
Size: 3929
BspMI ACCTGCnnnn'nnnn_
Cuts at: 289 761 979 1193 1495 1876 289
Size: 472 218 214 302 381 2342
Fragments arranged by size:
2342 472 381 302 218 214
BsrBI CCG'CTC
Cuts at: 50 2221 2275 3759 3924 50
Size: 2171 54 1484 165 55
Fragments arranged by size:
2171 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1841 1841
Size: 3929
BsrFI r'CCGG_y
Cuts at: 156 1927 2108 2317 156
Size: 1771 181 209 1768
Fragments arranged by size:
1771 1768 209 181
BssSI C'ACGA_G
Cuts at: 463 2200 2558 463
Size: 1737 358 1834
Fragments arranged by size:
1834 1737 358
BstAPI GCAn_nnn'nTGC
Cuts at: 281 384 1306 281
Size: 103 922 2904
Fragments arranged by size:
2904 922 103
BstBI TT'CG_AA
Cuts at: 2290 2290
Size: 3929
BstXI CCAn_nnnn'nTGG
Cuts at: 234 234
Size: 3929
BstYI r'GATC_y
Cuts at: 1 512 954 1421 1444 1779 2025 3037 3123
Size: 511 442 467 23 335 246 1012 86
Cuts at: 3123 3135 3481 1
Size: 12 346 449
Fragments arranged by size:
1012 511 467 449 442 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 122 396 807 107
Size: 15 274 411 3229
Fragments arranged by size:
3229 411 274 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1879 2060 2223 1879
Size: 181 163 3585
Fragments arranged by size:
3585 181 163
BtsI GCAGTG_nn'
Cuts at: 183 566 620 799 1172 183
Size: 383 54 179 373 2940
Fragments arranged by size:
2940 383 373 179 54
DraI TTT'AAA
Cuts at: 928 928
Size: 3929
DrdI GACnn_nn'nnGTC
Cuts at: 1635 2493 1635
Size: 858 3071
EaeI y'GGCC_r
Cuts at: 140 290 393 488 810 1514 1688 2079 2106
Size: 150 103 95 322 704 174 391 27
Cuts at: 2106 3451 140
Size: 1345 618
Fragments arranged by size:
1345 704 618 391 322 174 150 103
95 27
EagI C'GGCC_G
Cuts at: 393 1514 393
Size: 1121 2808
EarI CTCTTCn'nnn_
Cuts at: 1952 2162 1952
Size: 210 3719
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2137 2447 2593 3739 2137
Size: 310 146 1146 2327
Fragments arranged by size:
2327 1146 310 146
EcoICRI GAG'CTC
Cuts at: 714 941 714
Size: 227 3702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 568 575 1754 2186 2933 3201 568
Size: 7 1179 432 747 268 1296
Fragments arranged by size:
1296 1179 747 432 268 7
EcoNI CCTnn'n_nnAGG
Cuts at: 292 292
Size: 3929
EcoO109I rG'GnC_Cy
Cuts at: 675 676 1068 675
Size: 1 392 3536
Fragments arranged by size:
3536 392 1
EcoRI G'AATT_C
Cuts at: 933 933
Size: 3929
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1948 1948
Size: 3929
FseI GG_CCGG'CC
Cuts at: 160 160
Size: 3929
FspI TGC'GCA
Cuts at: 1710 1710
Size: 3929
HaeII r_GCGC'y
Cuts at: 327 1363 1611 2633 3612 327
Size: 1036 248 1022 979 644
Fragments arranged by size:
1036 1022 979 644 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 286 318 1771 1801 1803 1833 286
Size: 32 1453 30 2 30 2382
Fragments arranged by size:
2382 1453 32 30 30 2
HincII GTy'rAC
Cuts at: 879 968 879
Size: 89 3840
KasI G'GCGC_C
Cuts at: 1359 1607 1359
Size: 248 3681
KpnI G_GTAC'C
Cuts at: 949 949
Size: 3929
MfeI C'AATT_G
Cuts at: 759 759
Size: 3929
MluI A'CGCG_T
Cuts at: 3362 3362
Size: 3929
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2600 2784 2600
Size: 184 3745
MscI TGG'CCA
Cuts at: 1690 1690
Size: 3929
MslI CAynn'nnrTG
Cuts at: 1918 2045 1918
Size: 127 3802
MspA1I CmG'CkG
Cuts at: 124 375 398 1040 1354 1714 2727 2972 124
Size: 251 23 642 314 360 1013 245 1081
Fragments arranged by size:
1081 1013 642 360 314 251 245 23
NaeI GCC'GGC
Cuts at: 158 2110 158
Size: 1952 1977
NarI GG'CG_CC
Cuts at: 1360 1608 1360
Size: 248 3681
NcoI C'CATG_G
Cuts at: 107 107
Size: 3929
NgoMIV G'CCGG_C
Cuts at: 156 2108 156
Size: 1952 1977
NspI r_CATG'y
Cuts at: 982 1482 2389 982
Size: 500 907 2522
Fragments arranged by size:
2522 907 500
PasI CC'CwG_GG
Cuts at: 234 333 234
Size: 99 3830
PciI A'CATG_T
Cuts at: 2385 2385
Size: 3929
PflMI CCAn_nnn'nTGG
Cuts at: 168 168
Size: 3929
PmeI GTTT'AAAC
Cuts at: 928 928
Size: 3929
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3068 3100 3068
Size: 32 3897
PshAI GACnn'nnGTC
Cuts at: 663 663
Size: 3929
PspOMI G'GGCC_C
Cuts at: 675 675
Size: 3929
PstI C_TGCA'G
Cuts at: 710 976 1661 710
Size: 266 685 2978
Fragments arranged by size:
2978 685 266
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3929
PvuII CAG'CTG
Cuts at: 1354 1714 1354
Size: 360 3569
RsrII CG'GwC_CG
Cuts at: 2124 2124
Size: 3929
SacI G_AGCT'C
Cuts at: 716 943 716
Size: 227 3702
SacII CC_GC'GG
Cuts at: 125 399 125
Size: 274 3655
SalI G'TCGA_C
Cuts at: 966 966
Size: 3929
SapI GCTCTTCn'nnn_
Cuts at: 1952 2162 1952
Size: 210 3719
SbfI CC_TGCA'GG
Cuts at: 710 976 710
Size: 266 3663
ScaI AGT'ACT
Cuts at: 3446 3446
Size: 3929
SfcI C'TryA_G
Cuts at: 33 706 972 1657 2650 2841 33
Size: 673 266 685 993 191 1121
Fragments arranged by size:
1121 993 685 673 266 191
SfoI GGC'GCC
Cuts at: 1361 1609 1361
Size: 248 3681
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3929
SmaI CCC'GGG
Cuts at: 951 951
Size: 3929
SmlI C'TyrA_G
Cuts at: 1084 1999 2491 2753 1084
Size: 915 492 262 2260
Fragments arranged by size:
2260 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3929
SphI G_CATG'C
Cuts at: 982 1482 982
Size: 500 3429
StyI C'CwwG_G
Cuts at: 107 1063 1417 107
Size: 956 354 2619
Fragments arranged by size:
2619 956 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1512 1645 2104 2313 1512
Size: 133 459 209 3128
Fragments arranged by size:
3128 459 209 133
TatI w'GTAC_w
Cuts at: 3444 3444
Size: 3929
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 877 909 3446 3478 877
Size: 32 2537 32 1328
Fragments arranged by size:
2537 1328 32 32
Tth111I GACn'n_nGTC
Cuts at: 591 1726 591
Size: 1135 2794
XbaI T'CTAG_A
Cuts at: 960 960
Size: 3929
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 483 729 1424 483
Size: 246 695 2988
Fragments arranged by size:
2988 695 246
XmaI C'CCGG_G
Cuts at: 949 949
Size: 3929
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PmeI PpiI
PshAI PspOMI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BpmI
Bpu10I BsaXI BsiWI BsmI BsmBI BsrGI BssHII BstEII
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FspAI HindIII
HpaI NdeI NheI NotI NruI NsiI PacI PfoI
PmlI PpuMI PsiI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI StuI SwaI XhoI XmnI
ZraI