Restriction map for pF1KB4411
##################################
# NO CUT ENZYMES (  164 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     FauI        FbaI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MslI        
MunI        MvnI        MwoI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NspI        
PacI        PaeR7I      PflFI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        Tru9I       
Tth111I     XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AfaI          522  GTAC                 GT^AC
ApaI          548  GGGCCC               GGGCC^C
AvaI          413  CCCGGG               C^[CT]CG[GA]G
BbsI          138  GAAGAC               GAAGAC(2/6)
BbvCI         406  GCTGAGG              CCTCAGC(-5/-2)
BglII          68  AGATCT               A^GATCT
BpuAI         138  GAAGAC               GAAGAC(2/6)
BsaI           35  GAGACC               GGTCTC(1/5)
BsmFI         242  GTCCC                GGGAC(10/14)
BsoBI         413  CCCGGG               C^[CT]CG[GA]G
BsrFI         382  GCCGGC               [GA]^CCGG[CT]
BsrI          148  CCAGT                ACTGG(1/-1)
BstBI         478  TTCGAA               TT^CGAA
BstYI          68  AGATCT               [GA]^GATC[CT]
CfoI          424  GCGC                 GCG^C
Cfr10I        382  GCCGGC               [GA]^CCGG[CT]
DpnII          69  GATC                 ^GATC
EaeI          380  TGGCCG               [CT]^GGCC[GA]
EarI          178  CTCTTC               CTCTTC(1/4)
EcoT22I        21  ATGCAT               ATGCA^T
HaeII         423  GGCGCT               [GA]GCGC^[CT]
HhaI          424  GCGC                 GCG^C
HinP1I        424  GCGC                 G^CGC
Ksp632I       178  CTCTTC               CTCTTC(1/4)
MboI           69  GATC                 ^GATC
MflI           68  AGATCT               [GA]^GATC[CT]
NaeI          382  GCCGGC               GCC^GGC
NdeII          69  GATC                 ^GATC
NgoMIV        382  GCCGGC               G^CCGGC
NlaIII        419  CATG                 CATG^
NsiI           21  ATGCAT               ATGCA^T
NspV          478  TTCGAA               TT^CGAA
PflMI         432  CCAGGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          297  CTGCAG               CTGCA^G
RsaI          522  GTAC                 GT^AC
Sau3AI         69  GATC                 ^GATC
SfcI          297  CTGCAG               C^T[GA][CT]AG
SfuI          478  TTCGAA               TT^CGAA
SmaI          413  CCCGGG               CCC^GGG
TaqI          479  TCGA                 T^CGA
TfiI          283  GAATC                G^A[AT]TC
Tsp509I       230  AATT                 ^AATT
TthHB8I       479  TCGA                 T^CGA
Van91I        432  CCAGGGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           17  CCAAATGCATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII          68  AGATCT               [GA]^GATC[CT]
XmaCI         413  CCCGGG               C^CCGGG
XmaI          413  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AciI           56  CCGC                 CCGC(-3/-1)
               75  CCGC                
AlwNI         149  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              433  CAGGGCCTG           
BanII          86  GAGCCC               G[GA]GC[CT]^C
              548  GGGCCC              
BbvI           13  GCAGC                GCAGC(8/12)
              299  GCAGC               
BmyI           86  GAGCCC               G[AGT]GC[ACT]^C
              548  GGGCCC              
BpmI          332  CTCCAG               CTGGAG(16/14)
              310  CTGGAG              
Bsp1286I       86  GAGCCC               G[AGT]GC[ACT]^C
              548  GGGCCC              
BstF5I         24  CATCC                GGATG(2/0)
              455  CATCC               
DdeI          402  CTCAG                C^T[ACGT]AG
              407  CTGAG               
DraII         434  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              548  GGGCCCT             
EcoO109I      434  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              548  GGGCCCT             
Fnu4HI         13  GCAGC                GC^[ACGT]GC
              299  GCAGC               
FokI           24  CATCC                GGATG(9/13)
              455  CATCC               
HphI            3  GGTGA                GGTGA(8/7)
              135  GGTGA               
ItaI           13  GCAGC                GC^[ACGT]GC
              299  GCAGC               
MaeIII        271  GTGAC                ^GT[ACGT]AC
              324  GTCAC               
MnlI          409  GAGG                 CCTC(7/6)
              538  GAGG                
MspA1I        404  CAGCTG               C[AC]G^C[GT]G
              517  CAGCTG              
PvuII         404  CAGCTG               CAG^CTG
              517  CAGCTG              
SfaNI          23  GCATC                GCATC(5/9)
              338  GCATC               
TseI           13  GCAGC                G^C[AT]GC
              299  GCAGC               
Tsp45I        271  GTGAC                ^GT[GC]AC
              324  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AluI        BcnI        BsaJI       BsiYI       BslI        
BsmAI       BssKI       BstNI       Cac8I       Cfr13I      
EcoRII      HaeIII      HapII       HinfI       HpaII       
MboII       MspI        MvaI        NciI        NlaIV       
PleI        Sau96I      ScrFI       TspRI       
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