Restriction map for pF1KB4406
##################################
# NO CUT ENZYMES (   56 enzymes) #
##################################

AclI        AflII       AgeI        AhdI        AscI        
AspEI       AspI        AviII       AvrII       BbeI        
BfrI        BlnI        BsiEI       BsiWI       BsmBI       
BspDI       Bst1107I    BstZ17I     ClaI        CpoI        
EagI        Eam1105I    EclXI       Eco52I      EcoRI       
FseI        FspI        HpaI        KasI        KspI        
MluI        NarI        NotI        PacI        PaeR7I      
PflFI       PinAI       PmeI        PshAI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SfiI        SfoI        SgrAI       SnaBI       
SphI        Sse8387I    SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

Alw44I        319  GTGCAC               G^TGCAC
ApaI         4243  GGGCCC               GGGCC^C
ApaLI         319  GTGCAC               G^TGCAC
BamHI        3690  GGATCC               G^GATCC
BbrPI        4251  CACGTG               CAC^GTG
BbvCI        2523  CCTCAGC              CCTCAGC(-5/-2)
BsaBI        3685  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI         725  TCATGA               T^CATGA
BstBI        4426  TTCGAA               TT^CGAA
BstEII       1898  GGTCACC              G^GT[ACGT]ACC
BstPI        1898  GGTCACC              G^GT[ACGT]ACC
DrdI          281  GACAGGTTAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1898  GGTCACC              G^GT[ACGT]ACC
EcoT22I      6371  ATGCAT               ATGCA^T
MamI         3685  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI         1189  GCCGGC               GCC^GGC
NdeI         4794  CATATG               CA^TATG
NgoMIV       1189  GCCGGC               G^CCGGC
NheI         1543  GCTAGC               G^CTAGC
NruI           28  TCGCGA               TCG^CGA
NsiI         6371  ATGCAT               ATGCA^T
NspV         4426  TTCGAA               TT^CGAA
PmaCI        4251  CACGTG               CAC^GTG
PmlI         4251  CACGTG               CAC^GTG
RcaI          725  TCATGA               T^CATGA
SacI           74  GAGCTC               GAGCT^C
SfuI         4426  TTCGAA               TT^CGAA
SpeI         5179  ACTAGT               A^CTAGT
SspI         1043  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AatII        2428  GACGTC               GACGT^C
             6622  GACGTC              
Acc65I       4319  GGTACC               G^GTACC
             5736  GGTACC              
AccI         1267  GTCTAC               GT^[AC][GT]AC
             4768  GTCTAC              
AcyI         2428  GACGTC               G[GA]^CG[CT]C
             6622  GACGTC              
AocI         3181  CCTCAGG              CC^T[ACGT]AGG
             4929  CCTGAGG             
AseI         1036  ATTAAT               AT^TAAT
             1285  ATTAAT              
AsnI         1036  ATTAAT               AT^TAAT
             1285  ATTAAT              
Asp718I      4319  GGTACC               G^GTACC
             5736  GGTACC              
BcgI         3918  CGATGAGGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              341  GCAACGTGATCG        
BciVI        3660  GGATAC               GTATCC(6/5)
             3392  GTATCC              
BclI         2474  TGATCA               T^GATCA
             6108  TGATCA              
BsaHI        2428  GACGTC               G[GA]^CG[CT]C
             6622  GACGTC              
BsmI         1472  GAATGC               GAATGC(1/-1)
             4429  GAATGC              
BssHII        116  GCGCGC               G^CGCGC
              184  GCGCGC              
BssSI         145  CTCGTG               CACGAG(-5/-1)
             2647  CTCGTG              
BstAPI         96  GCAGAGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             6076  GCACCTGATGC         
Bsu36I       3181  CCTCAGG              CC^T[ACGT]AGG
             4929  CCTGAGG             
DraI         6141  TTTAAA               TTT^AAA
             6244  TTTAAA              
DraIII       1243  CACCGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2740  CACCTGGTG           
Eco81I       3181  CCTCAGG              CC^T[ACGT]AGG
             4929  CCTGAGG             
EcoRV        5254  GATATC               GAT^ATC
             5314  GATATC              
FbaI         2474  TGATCA               T^GATCA
             6108  TGATCA              
HgaI           93  GACGC                GACGC(5/10)
              541  GACGC               
Hin1I        2428  GACGTC               G[GA]^CG[CT]C
             6622  GACGTC              
HindIII      2504  AAGCTT               A^AGCTT
             5289  AAGCTT              
KpnI         4319  GGTACC               GGTAC^C
             5736  GGTACC              
PshBI        1036  ATTAAT               AT^TAAT
             1285  ATTAAT              
SapI         1776  GAAGAGC              GCTCTTC(1/4)
             5655  GAAGAGC             
SexAI        1070  ACCAGGT              A^CC[AT]GGT
             2741  ACCTGGT             
SmaI          857  CCCGGG               CCC^GGG
             2033  CCCGGG              
XmaCI         857  CCCGGG               C^CCGGG
             2033  CCCGGG              
XmaI          857  CCCGGG               C^CCGGG
             2033  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  141 enzymes) #
#########################################

AccII       AccIII      AciI        AcsI        AfaI        
AflIII      AluI        AlwI        AlwNI       Aor51HI     
ApoI        Asp700I     AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BglI        BglII       BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspEI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CelII       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        Eco47III    
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MfeI        
MflI        MluNI       MnlI        MroI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        SspBI       StuI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmnI        
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