Restriction map for pF1KB4406
 (Linear) (Six-base) MAPSORT of: KIBB4406.seq  Check: 5618  from: 1  to: 6642



KIBB4406.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    3110    5538    5819    6642 
   Size:       3110    2428     281     823

  Fragments arranged by size: 

                3110    2428     823     281
 
AatII G_ACGT'C

Cuts at:      0    2432    6626    6642 
   Size:       2432    4194      16

  Fragments arranged by size: 

                4194    2432      16
 
AccI GT'mk_AC

Cuts at:      0    1268    4769    6642 
   Size:       1268    3501    1873

  Fragments arranged by size: 

                3501    1873    1268
 
Acc65I G'GTAC_C

Cuts at:      0    4319    5736    6642 
   Size:       4319    1417     906

  Fragments arranged by size: 

                4319    1417     906
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1159    1342    2713    4666    6280    6642 
   Size:       1159     183    1371    1953    1614     362

  Fragments arranged by size: 

                1953    1614    1371    1159     362     183
 
AfeI AGC'GCT

Cuts at:      0     246    2857    4468    4597    6642 
   Size:        246    2611    1611     129    2045

  Fragments arranged by size: 

                2611    2045    1611     246     129
 
AflIII A'CryG_T

Cuts at:      0     407     738     917    1556    4252    4447    6185    6278 
   Size:        407     331     179     639    2696     195    1738      93

Cuts at:   6278    6642 
   Size:        364

  Fragments arranged by size: 

                2696    1738     639     407     364     331     195     179
                  93
 
AleI CACnn'nnGTG

Cuts at:      0    1204    3593    4306    6642 
   Size:       1204    2389     713    2336

  Fragments arranged by size: 

                2389    2336    1204     713
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2906    6642 
   Size:       2906    3736
 
AlwNI CAG_nnn'CTG

Cuts at:      0     102    1768    3554    3867    6079    6642 
   Size:        102    1666    1786     313    2212     563

  Fragments arranged by size: 

                2212    1786    1666     563     313     102
 
ApaI G_GGCC'C

Cuts at:      0    4247    6642 
   Size:       4247    2395
 
ApaLI G'TGCA_C

Cuts at:      0     319    6642 
   Size:        319    6323
 
ApoI r'AATT_y

Cuts at:      0     440    2110    6248    6642 
   Size:        440    1670    4138     394

  Fragments arranged by size: 

                4138    1670     440     394
 
AseI AT'TA_AT

Cuts at:      0    1037    1286    6642 
   Size:       1037     249    5356

  Fragments arranged by size: 

                5356    1037     249
 
AvaI C'yCGr_G

Cuts at:      0     259     362     857    2033    2325    6642 
   Size:        259     103     495    1176     292    4317

  Fragments arranged by size: 

                4317    1176     495     292     259     103
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    6172    6205    6642 
   Size:       6172      33     437

  Fragments arranged by size: 

                6172     437      33
 
BamHI G'GATC_C

Cuts at:      0    3690    6642 
   Size:       3690    2952
 
BanI G'GyrC_C

Cuts at:      0    4319    5736    6420    6642 
   Size:       4319    1417     684     222

  Fragments arranged by size: 

                4319    1417     684     222
 
BanII G_rGCy'C

Cuts at:      0      78     266    2640    3011    3818    4247    5022    6642 
   Size:         78     188    2374     371     807     429     775    1620

  Fragments arranged by size: 

                2374    1620     807     775     429     371     188      78
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     822     956    1779    3226    4450    4807    6642 
   Size:        822     134     823    1447    1224     357    1835

  Fragments arranged by size: 

                1835    1447    1224     823     822     357     134
 
BbvCI CC'TCA_GC

Cuts at:      0    2524    6642 
   Size:       2524    4118
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     330     364    3907    3941    6642 
   Size:        330      34    3543      34    2701

  Fragments arranged by size: 

                3543    2701     330      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3403    3654    6642 
   Size:       3403     251    2988

  Fragments arranged by size: 

                3403    2988     251
 
BclI T'GATC_A

Cuts at:      0    2474    6108    6642 
   Size:       2474    3634     534

  Fragments arranged by size: 

                3634    2474     534
 
BfrBI ATG'CAT

Cuts at:      0    6373    6642 
   Size:       6373     269
 
BglI GCCn_nnn'nGGC

Cuts at:      0     202    3185    3737    4496    6308    6642 
   Size:        202    2983     552     759    1812     334

  Fragments arranged by size: 

                2983    1812     759     552     334     202
 
BglII A'GATC_T

Cuts at:      0    5910    6317    6394    6642 
   Size:       5910     407      77     248

  Fragments arranged by size: 

                5910     407     248      77
 
BlpI GC'TnA_GC

Cuts at:      0    1433    2941    6445    6642 
   Size:       1433    1508    3504     197

  Fragments arranged by size: 

                3504    1508    1433     197
 
Bme1580I G_kGCm'C

Cuts at:      0     323    2654    3068    3629    4247    6425    6642 
   Size:        323    2331     414     561     618    2178     217

  Fragments arranged by size: 

                2331    2178     618     561     414     323     217
 
BmgBI CAC'GTC

Cuts at:      0    4448    5627    6642 
   Size:       4448    1179    1015

  Fragments arranged by size: 

                4448    1179    1015
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     333     489     546    1008    2550    2691    2931    3357 
   Size:        333     156      57     462    1542     141     240     426

Cuts at:   3357    5025    6031    6642 
   Size:       1668    1006     611

  Fragments arranged by size: 

                1668    1542    1006     611     462     426     333     240
                 156     141      57
 
BmtI G_CTAG'C

Cuts at:      0    1547    6642 
   Size:       1547    5095
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350    1349    1444    1484    1598    1822    2512    2839 
   Size:        350     999      95      40     114     224     690     327

Cuts at:   2839    4828    4954    5220    6519    6642 
   Size:       1989     126     266    1299     123

  Fragments arranged by size: 

                1989    1299     999     690     350     327     266     224
                 126     123     114      95      40
 
Bpu10I CC'TnA_GC

Cuts at:      0    2524    6642 
   Size:       2524    4118
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     901    3181    3439    5527    6642 
   Size:        901    2280     258    2088    1115

  Fragments arranged by size: 

                2280    2088    1115     901     258
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     987    1622    1699    2681    4804    6642 
   Size:        987     635      77     982    2123    1838

  Fragments arranged by size: 

                2123    1838     987     982     635      77
 
BsaAI yAC'GTr

Cuts at:      0     408    4253    4865    6642 
   Size:        408    3845     612    1777

  Fragments arranged by size: 

                3845    1777     612     408
 
BsaBI GATnn'nnATC

Cuts at:      0    3689    6642 
   Size:       3689    2953
 
BsaHI Gr'CG_yC

Cuts at:      0    2429    6623    6642 
   Size:       2429    4194      19

  Fragments arranged by size: 

                4194    2429      19
 
BsaWI w'CCGG_w

Cuts at:      0     230    1466    2106    2183    6485    6642 
   Size:        230    1236     640      77    4302     157

  Fragments arranged by size: 

                4302    1236     640     230     157      77
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    2204    2231    2234    2261    4543    4573    6517    6547 
   Size:       2204      27       3      27    2282      30    1944      30

Cuts at:   6547    6642 
   Size:         95

  Fragments arranged by size: 

                2282    2204    1944      95      30      30      27      27
                   3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     616    1318    1441    1747    2228    3507    5012    5391 
   Size:        616     702     123     306     481    1279    1505     379

Cuts at:   5391    6549    6642 
   Size:       1158      93

  Fragments arranged by size: 

                1505    1279    1158     702     616     481     379     306
                 123      93
 
BseYI C'CCAG_C

Cuts at:      0    1932    3638    6354    6540    6642 
   Size:       1932    1706    2716     186     102

  Fragments arranged by size: 

                2716    1932    1706     186     102
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      88     172    1857    2013    4352    5516    6386    6642 
   Size:         88      84    1685     156    2339    1164     870     256

  Fragments arranged by size: 

                2339    1685    1164     870     256     156      88      84
            
 
BsiHKAI G_wGCw'C

Cuts at:      0      78     323    1169    1783    2396    4589    6642 
   Size:         78     245     846     614     613    2193    2053

  Fragments arranged by size: 

                2193    2053     846     614     613     245      78
 
BsmI GAATG_Cn'

Cuts at:      0    1478    4435    6642 
   Size:       1478    2957    2207

  Fragments arranged by size: 

                2957    2207    1478
 
Bsp1286I G_dGCh'C

Cuts at:      0      78     266     323    1169    1783    2396    2640    2654 
   Size:         78     188      57     846     614     613     244      14

Cuts at:   2654    3011    3068    3629    3818    4247    4589    5022    6425 
   Size:        357      57     561     189     429     342     433    1403

Cuts at:   6425    6642 
   Size:        217

  Fragments arranged by size: 

                1403     846     614     613     561     433     429     357
                 342     244     217     189     188      78      57      57
                  14
 
BspEI T'CCGG_A

Cuts at:      0     230    1466    2106    6642 
   Size:        230    1236     640    4536

  Fragments arranged by size: 

                4536    1236     640     230
 
BspHI T'CATG_A

Cuts at:      0     725    6642 
   Size:        725    5917
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    3110    4297    5538    5819    6642 
   Size:       3110    1187    1241     281     823

  Fragments arranged by size: 

                3110    1241    1187     823     281
 
BsrBI CCG'CTC

Cuts at:      0     228    1914    2832    3515    6642 
   Size:        228    1686     918     683    3127

  Fragments arranged by size: 

                3127    1686     918     683     228
 
BsrDI GCAATG_nn'

Cuts at:      0    1271    1652    2894    3060    3723    6642 
   Size:       1271     381    1242     166     663    2919

  Fragments arranged by size: 

                2919    1271    1242     663     381     166
 
BsrFI r'CCGG_y

Cuts at:      0    1189    2004    5587    6642 
   Size:       1189     815    3583    1055

  Fragments arranged by size: 

                3583    1189    1055     815
 
BsrGI T'GTAC_A

Cuts at:      0    1559    4664    6473    6642 
   Size:       1559    3105    1809     169

  Fragments arranged by size: 

                3105    1809    1559     169
 
BssHII G'CGCG_C

Cuts at:      0     116     184    6642 
   Size:        116      68    6458

  Fragments arranged by size: 

                6458     116      68
 
BssSI C'ACGA_G

Cuts at:      0     145    2647    6642 
   Size:        145    2502    3995

  Fragments arranged by size: 

                3995    2502     145
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     102    6082    6642 
   Size:        102    5980     560

  Fragments arranged by size: 

                5980     560     102
 
BstBI TT'CG_AA

Cuts at:      0    4427    6642 
   Size:       4427    2215
 
BstEII G'GTnAC_C

Cuts at:      0    1898    6642 
   Size:       1898    4744
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    2640    3382    5038    6322    6642 
   Size:       2640     742    1656    1284     320

  Fragments arranged by size: 

                2640    1656    1284     742     320
 
BstYI r'GATC_y

Cuts at:      0    2103    3690    3951    5910    6317    6394    6642 
   Size:       2103    1587     261    1959     407      77     248

  Fragments arranged by size: 

                2103    1959    1587     407     261     248      77
 
Bsu36I CC'TnA_GG

Cuts at:      0    3182    4930    6642 
   Size:       3182    1748    1712

  Fragments arranged by size: 

                3182    1748    1712
 
BtgI C'CryG_G

Cuts at:      0    1632    1850    3878    5037    5303    5840    6629    6642 
   Size:       1632     218    2028    1159     266     537     789      13

  Fragments arranged by size: 

                2028    1632    1159     789     537     266     218      13
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1035    1497    3335    4422    4646    6642 
   Size:       1035     462    1838    1087     224    1996

  Fragments arranged by size: 

                1996    1838    1087    1035     462     224
 
BtsI GCAGTG_nn'

Cuts at:      0      58     402     957    1200    1578    2375    3150    3627 
   Size:         58     344     555     243     378     797     775     477

Cuts at:   3627    3807    3868    4137    4287    4376    4653    5576    6642 
   Size:        180      61     269     150      89     277     923    1066

  Fragments arranged by size: 

                1066     923     797     775     555     477     378     344
                 277     269     243     180     150      89      61      58
            
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2949    2984    3288    3323    4383    4418    4468    4503 
   Size:       2949      35     304      35    1060      35      50      35

Cuts at:   4503    6642 
   Size:       2139

  Fragments arranged by size: 

                2949    2139    1060     304      50      35      35      35
                  35
 
DraI TTT'AAA

Cuts at:      0    6143    6246    6642 
   Size:       6143     103     396

  Fragments arranged by size: 

                6143     396     103
 
DraIII CAC_nnn'GTG

Cuts at:      0    1248    2745    6642 
   Size:       1248    1497    3897

  Fragments arranged by size: 

                3897    1497    1248
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     287    6642 
   Size:        287    6355
 
EaeI y'GGCC_r

Cuts at:      0     711     990    1649    2847    4134    4476    4497    6642 
   Size:        711     279     659    1198    1287     342      21    2145

  Fragments arranged by size: 

                2145    1287    1198     711     659     342     279      21
            
 
EarI CTCTTCn'nnn_

Cuts at:      0     593    1771    2261    3215    5650    6642 
   Size:        593    1178     490     954    2435     992

  Fragments arranged by size: 

                2435    1178     992     954     593     490
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1652    1974    6642 
   Size:       1652     322    4668

  Fragments arranged by size: 

                4668    1652     322
 
EcoICRI GAG'CTC

Cuts at:      0      76    6642 
   Size:         76    6566
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350    1159    1342    1349    1444    1484    1598    1822 
   Size:        350     809     183       7      95      40     114     224

Cuts at:   1822    2512    2713    2839    4666    4828    4954    5220    6280 
   Size:        690     201     126    1827     162     126     266    1060

Cuts at:   6280    6519    6642 
   Size:        239     123

  Fragments arranged by size: 

                1827    1060     809     690     350     266     239     224
                 201     183     162     126     126     123     114      95
                  40       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1206    1486    3094    4725    5100    6642 
   Size:       1206     280    1608    1631     375    1542

  Fragments arranged by size: 

                1631    1608    1542    1206     375     280
 
EcoO109I rG'GnC_Cy

Cuts at:      0     671    2972    3162    4244    4286    4934    6642 
   Size:        671    2301     190    1082      42     648    1708

  Fragments arranged by size: 

                2301    1708    1082     671     648     190      42
 
EcoRV GAT'ATC

Cuts at:      0    5256    5316    6642 
   Size:       5256      60    1326

  Fragments arranged by size: 

                5256    1326      60
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     474     895    5490    6155    6642 
   Size:        474     421    4595     665     487

  Fragments arranged by size: 

                4595     665     487     474     421
 
HaeII r_GCGC'y

Cuts at:      0     248    1440    2442    2859    4470    4599    6642 
   Size:        248    1192    1002     417    1611     129    2043

  Fragments arranged by size: 

                2043    1611    1192    1002     417     248     129
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1307    1339    1376    1408    2231    2263    2308    2340 
   Size:       1307      32      37      32     823      32      45      32

Cuts at:   2340    2588    2620    2917    2949    3121    3153    3995    4027 
   Size:        248      32     297      32     172      32     842      32

Cuts at:   4027    5197    5229    6343    6375    6642 
   Size:       1170      32    1114      32     267

  Fragments arranged by size: 

                1307    1170    1114     842     823     297     267     248
                 172      45      37      32      32      32      32      32
                  32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    3699    4658    5145    6642 
   Size:       3699     959     487    1497

  Fragments arranged by size: 

                3699    1497     959     487
 
HindIII A'AGCT_T

Cuts at:      0    2504    5289    6642 
   Size:       2504    2785    1353

  Fragments arranged by size: 

                2785    2504    1353
 
KpnI G_GTAC'C

Cuts at:      0    4323    5740    6642 
   Size:       4323    1417     902

  Fragments arranged by size: 

                4323    1417     902
 
MfeI C'AATT_G

Cuts at:      0    1395    4512    5897    6642 
   Size:       1395    3117    1385     745

  Fragments arranged by size: 

                3117    1395    1385     745
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     671    2042    2438    2619    3629    4764    5966    6042 
   Size:        671    1371     396     181    1010    1135    1202      76

Cuts at:   6042    6278    6642 
   Size:        236     364

  Fragments arranged by size: 

                1371    1202    1135    1010     671     396     364     236
                 181      76
 
MscI TGG'CCA

Cuts at:      0     713    2849    4136    6642 
   Size:        713    2136    1287    2506

  Fragments arranged by size: 

                2506    2136    1287     713
 
MslI CAynn'nnrTG

Cuts at:      0     271    1204    2779    2888    3016    3593    3767    4306 
   Size:        271     933    1575     109     128     577     174     539

Cuts at:   4306    5036    5813    6158    6642 
   Size:        730     777     345     484

  Fragments arranged by size: 

                1575     933     777     730     577     539     484     345
                 271     174     128     109
 
MspA1I CmG'CkG

Cuts at:      0     619     690    1826    2532    3642    3728    4274    4693 
   Size:        619      71    1136     706    1110      86     546     419

Cuts at:   4693    4974    6642 
   Size:        281    1668

  Fragments arranged by size: 

                1668    1136    1110     706     619     546     419     281
                  86      71
 
NaeI GCC'GGC

Cuts at:      0    1191    6642 
   Size:       1191    5451
 
NcoI C'CATG_G

Cuts at:      0    3878    5037    5303    6629    6642 
   Size:       3878    1159     266    1326      13

  Fragments arranged by size: 

                3878    1326    1159     266      13
 
NdeI CA'TA_TG

Cuts at:      0    4795    6642 
   Size:       4795    1847
 
NgoMIV G'CCGG_C

Cuts at:      0    1189    6642 
   Size:       1189    5453
 
NheI G'CTAG_C

Cuts at:      0    1543    6642 
   Size:       1543    5099
 
NruI TCG'CGA

Cuts at:      0      30    6642 
   Size:         30    6612
 
NsiI A_TGCA'T

Cuts at:      0    6375    6642 
   Size:       6375     267
 
NspI r_CATG'y

Cuts at:      0     742     921    1979    3507    5506    5818    6189    6642 
   Size:        742     179    1058    1528    1999     312     371     453

  Fragments arranged by size: 

                1999    1528    1058     742     453     371     312     179
            
 
PasI CC'CwG_GG

Cuts at:      0    1410    1659    2633    4985    6642 
   Size:       1410     249     974    2352    1657

  Fragments arranged by size: 

                2352    1657    1410     974     249
 
PciI A'CATG_T

Cuts at:      0     738     917    6185    6642 
   Size:        738     179    5268     457

  Fragments arranged by size: 

                5268     738     457     179
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1248    1665    3879    4154    5090    6642 
   Size:       1248     417    2214     275     936    1552

  Fragments arranged by size: 

                2214    1552    1248     936     417     275
 
PfoI T'CCnGG_A

Cuts at:      0    1602    4610    5783    6642 
   Size:       1602    3008    1173     859

  Fragments arranged by size: 

                3008    1602    1173     859
 
PmlI CAC'GTG

Cuts at:      0    4253    6642 
   Size:       4253    2389
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2260    2292    6642 
   Size:       2260      32    4350

  Fragments arranged by size: 

                4350    2260      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     671    2972    3162    4286    4934    6642 
   Size:        671    2301     190    1124     648    1708

  Fragments arranged by size: 

                2301    1708    1124     671     648     190
 
PspOMI G'GGCC_C

Cuts at:      0    4243    6642 
   Size:       4243    2399
 
PstI C_TGCA'G

Cuts at:      0     257    3121    3256    3496    3550    4279    4648    5571 
   Size:        257    2864     135     240      54     729     369     923

Cuts at:   5571    6497    6642 
   Size:        926     145

  Fragments arranged by size: 

                2864     926     923     729     369     257     240     145
                 135      54
 
PvuII CAG'CTG

Cuts at:      0     619     690    1826    2532    4693    4974    6642 
   Size:        619      71    1136     706    2161     281    1668

  Fragments arranged by size: 

                2161    1668    1136     706     619     281      71
 
SacI G_AGCT'C

Cuts at:      0      78    6642 
   Size:         78    6564
 
SanDI GG'GwC_CC

Cuts at:      0    2972    6642 
   Size:       2972    3670
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1771    5650    6642 
   Size:       1771    3879     992

  Fragments arranged by size: 

                3879    1771     992
 
SexAI A'CCwGG_T

Cuts at:      0    1070    2741    6642 
   Size:       1070    1671    3901

  Fragments arranged by size: 

                3901    1671    1070
 
SfcI C'TryA_G

Cuts at:      0     253    3117    3252    3492    3546    4275    4644    4773 
   Size:        253    2864     135     240      54     729     369     129

Cuts at:   4773    5567    6493    6528    6642 
   Size:        794     926      35     114

  Fragments arranged by size: 

                2864     926     794     729     369     253     240     135
                 129     114      54      35
 
SmaI CCC'GGG

Cuts at:      0     859    2035    6642 
   Size:        859    1176    4607

  Fragments arranged by size: 

                4607    1176     859
 
SmlI C'TyrA_G

Cuts at:      0     880    3196    3418    5542    6642 
   Size:        880    2316     222    2124    1100

  Fragments arranged by size: 

                2316    2124    1100     880     222
 
SpeI A'CTAG_T

Cuts at:      0    5179    6642 
   Size:       5179    1463
 
SspI AAT'ATT

Cuts at:      0    1045    6642 
   Size:       1045    5597
 
StuI AGG'CCT

Cuts at:      0     643    1505    3601    4576    4642    6642 
   Size:        643     862    2096     975      66    2000

  Fragments arranged by size: 

                2096    2000     975     862     643      66
 
StyI C'CwwG_G

Cuts at:      0     356    1154    1202    1487    1859    2423    3095    3878 
   Size:        356     798      48     285     372     564     672     783

Cuts at:   3878    5037    5303    6219    6629    6642 
   Size:       1159     266     916     410      13

  Fragments arranged by size: 

                1159     916     798     783     672     564     410     372
                 356     285     266      48      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1308    3376    4296    4411    4430    5050    5377    6642 
   Size:       1308    2068     920     115      19     620     327    1265

  Fragments arranged by size: 

                2068    1308    1265     920     620     327     115      19
            
 
TatI w'GTAC_w

Cuts at:      0     838     914    1559    3485    4664    4671    4878    5054 
   Size:        838      76     645    1926    1179       7     207     176

Cuts at:   5054    6473    6642 
   Size:       1419     169

  Fragments arranged by size: 

                1926    1419    1179     838     645     207     176     169
                  76       7
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1014    1046    2032    2064    2746    2776    2778    2808 
   Size:       1014      32     986      32     682      30       2      30

Cuts at:   2808    3154    3186    3829    3861    4455    4487    4543    4575 
   Size:        346      32     643      32     594      32      56      32

Cuts at:   4575    5240    5272    6517    6549    6642 
   Size:        665      32    1245      32      93

  Fragments arranged by size: 

                1245    1014     986     682     665     643     594     346
                  93      56      32      32      32      32      32      32
                  32      32      30      30       2
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     654    1524    1857    3351    3997    5472    6261    6642 
   Size:        654     870     333    1494     646    1475     789     381

  Fragments arranged by size: 

                1494    1475     870     789     654     646     381     333
            
 
XmaI C'CCGG_G

Cuts at:      0     857    2033    6642 
   Size:        857    1176    4609

  Fragments arranged by size: 

                4609    1176     857
 
XmnI GAAnn'nnTTC

Cuts at:      0    1717    2275    3025    5344    6642 
   Size:       1717     558     750    2319    1298

  Fragments arranged by size: 

                2319    1717    1298     750     558
 
ZraI GAC'GTC

Cuts at:      0    2430    6624    6642 
   Size:       2430    4194      18

  Fragments arranged by size: 

                4194    2430      18
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AcuI     AfeI   AflIII     AleI
     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI     AvaI     BaeI
    BamHI     BanI    BanII     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI    HaeII    Hin4I
   HincII  HindIII     KpnI     MfeI     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI     NdeI   NgoMIV     NheI     NruI     NsiI     NspI
     PasI     PciI    PflMI     PfoI     PmlI     PpiI    PpuMI   PspOMI
     PstI    PvuII     SacI    SanDI     SapI    SexAI     SfcI     SmaI
     SmlI     SpeI     SspI     StuI     StyI    TaqII     TatI     TstI
     XcmI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AclI    AflII     AgeI     AhdI     AloI     AscI    AsiSI    AvrII
     BbeI     BplI    BsiEI    BsiWI    BsmBI  BstZ17I     ClaI     EagI
    EcoRI     FseI     FspI    FspAI     HpaI     KasI     MluI     NarI
     NotI     PacI     PmeI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SphI     SrfI     SwaI  Tth111I     XbaI     XhoI
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