Restriction map for pF1KB4244
##################################
# NO CUT ENZYMES (  162 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AciI        
AclI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaII       AviII       AvrII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaAI       
BsaBI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HgaI        HhaI        HinP1I      HincII      HindII      
HindIII     HpaI        HphI        KasI        KpnI        
KspI        MamI        MluI        MluNI       MroI        
MscI        MslI        MvnI        NaeI        NarI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SphI        Sse8387I    SspBI       StuI        SwaI        
TaqI        Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII         115  GACGTC               GACGT^C
AcyI          115  GACGTC               G[GA]^CG[CT]C
AfaI          223  GTAC                 GT^AC
AocI          576  CCTTAGG              CC^T[ACGT]AGG
AseI          757  ATTAAT               AT^TAAT
AsnI          757  ATTAAT               AT^TAAT
AvaI          175  CCCGGG               C^[CT]CG[GA]G
BanII         384  GAGCCC               G[GA]GC[CT]^C
BbsI            6  GAAGAC               GAAGAC(2/6)
BfaI          479  CTAG                 C^TAG
BmyI          384  GAGCCC               G[AGT]GC[ACT]^C
BpuAI           6  GAAGAC               GAAGAC(2/6)
BsaHI         115  GACGTC               G[GA]^CG[CT]C
BsmFI         172  GTCCC                GGGAC(10/14)
BsoBI         175  CCCGGG               C^[CT]CG[GA]G
Bsp1286I      384  GAGCCC               G[AGT]GC[ACT]^C
BsrDI         656  CATTGC               GCAATG(2/0)
Bsu36I        576  CCTTAGG              CC^T[ACGT]AGG
DrdI          719  GACAGCCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          650  CCATGG               C^C[GA][CT]GG
Eco81I        576  CCTTAGG              CC^T[ACGT]AGG
EcoRI         415  GAATTC               G^AATTC
EcoT14I       650  CCATGG               C^C[AT][AT]GG
HaeIII        609  GGCC                 GG^CC
HapII         176  CCGG                 C^CGG
Hin1I         115  GACGTC               G[GA]^CG[CT]C
HpaII         176  CCGG                 C^CGG
MaeI          479  CTAG                 C^TAG
MaeII         116  ACGT                 A^CGT
MfeI          226  CAATTG               C^AATTG
MspA1I         43  CAGCTG               C[AC]G^C[GT]G
MspI          176  CCGG                 C^CGG
MunI          226  CAATTG               C^AATTG
NcoI          650  CCATGG               C^CATGG
NlaIV         697  GGAGCC               GG[ACGT]^[ACGT]CC
PshBI         757  ATTAAT               AT^TAAT
PstI           84  CTGCAG               CTGCA^G
PvuII          43  CAGCTG               CAG^CTG
RsaI          223  GTAC                 GT^AC
SfcI           84  CTGCAG               C^T[GA][CT]AG
SmaI          175  CCCGGG               CCC^GGG
SmlI          282  CTTGAG               C^T[CT][GA]AG
SpeI          478  ACTAGT               A^CTAGT
SspI          268  AATATT               AAT^ATT
StyI          650  CCATGG               C^C[AT][AT]GG
XcmI          193  CCAAGTCATTGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI         175  CCCGGG               C^CCGGG
XmaI          175  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcsI          415  GAATTC               [GA]^AATT[CT]
              425  GAATTT              
ApoI          415  GAATTC               [GA]^AATT[CT]
              425  GAATTT              
BcnI          175  CCCGG                CC^[GC]GG
              176  CCGGG               
BpmI          630  CTCCAG               CTGGAG(16/14)
              623  CTGGAG              
BsmAI         626  GAGAC                GTCTC(1/5)
              717  GAGAC               
BsrI          641  ACTGG                ACTGG(1/-1)
              707  ACTGG               
BstNI         395  CCAGG                CC^[AT]GG
              622  CCTGG               
Cac8I         568  GCCAGC               GC[ACGT]^[ACGT]GC
              660  GCCAGC              
EarI          714  GAAGAG               CTCTTC(1/4)
              763  GAAGAG              
EcoRII        395  CCAGG                ^CC[AT]GG
              622  CCTGG               
Ksp632I       714  GAAGAG               CTCTTC(1/4)
              763  GAAGAG              
MvaI          395  CCAGG                CC^[AT]GG
              622  CCTGG               
NciI          175  CCCGG                CC^[GC]GG
              176  CCGGG               
NlaIII        153  CATG                 CATG^
              651  CATG                
PleI          628  GACTC                GAGTC(4/5)
              527  GAGTC               
SfaNI         445  GATGC                GCATC(5/9)
              469  GCATC               
TspRI         531  CAGTG                CA[GC]TG[ACGT][ACGT]^
              640  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AluI        AlwI        BbvI        BsaJI       BsiYI       
BslI        BssKI       BstF5I      BstYI       DdeI        
DpnII       Fnu4HI      FokI        HinfI       ItaI        
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MwoI        NdeII       Sau3AI      ScrFI       
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
XhoII       
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