Restriction map for pF1KB4182
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AlwNI       Aor51HI     
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AviII       AvrII       BalI        
BamHI       BbrPI       BbsI        BcgI        BciVI       
BfaI        BfrI        BlnI        BpuAI       BsaBI       
BsaI        BsaWI       BseAI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstXI       BstZ17I     Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
Eco47III    EcoNI       EcoRI       EcoRV       EcoT22I     
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        MaeI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MseI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SalI        
SapI        SbfI        ScaI        SexAI       SfcI        
SfiI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        TfiI        
Tru9I       Tsp509I     Tth111I     XbaI        XcmI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII         658  GACGTC               GACGT^C
AhdI          181  GACGGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I         34  GTGCAC               G^TGCAC
AlwI          555  GGATC                GGATC(4/5)
AocI          941  CCTCAGG              CC^T[ACGT]AGG
ApaLI          34  GTGCAC               G^TGCAC
AspEI         181  GACGGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI          34  GTGCAC               G[AT]GC[AT]^C
BbeI          730  GGCGCC               GGCGC^C
BbvCI         663  CCTCAGC              CCTCAGC(-5/-2)
BclI           77  TGATCA               T^GATCA
BglI          766  GCCGTGGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         564  AGATCT               A^GATCT
BlpI          881  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      881  GCTCAGC              GC^T[ACGT]AGC
BsaAI         588  CACGTA               [CT]AC^GT[GA]
BsgI          936  CTGCAC               GTGCAG(16/14)
BsiEI          68  CGGCCG               CG[GA][CT]^CG
BsiHKAI        34  GTGCAC               G[AT]GC[AT]^C
BsmBI         131  GAGACG               CGTCTC(1/5)
BsmI          342  GAATGC               GAATGC(1/-1)
Bsu36I        941  CCTCAGG              CC^T[ACGT]AGG
CelII         881  GCTCAGC              GC^T[ACGT]AGC
EagI           68  CGGCCG               C^GGCCG
Eam1105I      181  GACGGCGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          207  CTCTTC               CTCTTC(1/4)
EclXI          68  CGGCCG               C^GGCCG
Eco52I         68  CGGCCG               C^GGCCG
Eco81I        941  CCTCAGG              CC^T[ACGT]AGG
FbaI           77  TGATCA               T^GATCA
HgaI          133  GACGC                GACGC(5/10)
KasI          730  GGCGCC               G^GCGCC
Ksp632I       207  CTCTTC               CTCTTC(1/4)
MslI          718  CATGGCGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI          730  GGCGCC               GG^CGCC
NcoI          717  CCATGG               C^CATGG
PflMI         834  CCATGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         689  GGGACCC              [GA]G^G[AT]CC[CT]
PvuII         933  CAGCTG               CAG^CTG
SfoI          730  GGCGCC               GGC^GCC
SmaI          992  CCCGGG               CCC^GGG
SphI          603  GCATGC               GCATG^C
StuI          545  AGGCCT               AGG^CCT
TspRI         485  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        834  CCATGCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         992  CCCGGG               C^CCGGG
XmaI          992  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccII         381  CGCG                 CG^CG
              507  CGCG                
AfaI          591  GTAC                 GT^AC
              762  GTAC                
ApaI          640  GGGCCC               GGGCC^C
              989  GGGCCC              
BsiWI         590  CGTACG               C^GTACG
              761  CGTACG              
BsrBI          56  GAGCGG               CCGCTC(-3/-3)
              286  GAGCGG              
BsrI           18  ACTGG                ACTGG(1/-1)
              395  CCAGT               
BstEII        273  GGTGACC              G^GT[ACGT]ACC
              712  GGTTACC             
BstPI         273  GGTGACC              G^GT[ACGT]ACC
              712  GGTTACC             
BstUI         381  CGCG                 CG^CG
              507  CGCG                
BstYI         555  GGATCT               [GA]^GATC[CT]
              564  AGATCT              
EaeI           68  CGGCCG               [CT]^GGCC[GA]
              120  TGGCCG              
EcoO65I       273  GGTGACC              G^GT[ACGT]ACC
              712  GGTTACC             
KspI          380  CCGCGG               CCGC^GG
              506  CCGCGG              
MflI          555  GGATCT               [GA]^GATC[CT]
              564  AGATCT              
MvnI          381  CGCG                 CG^CG
              507  CGCG                
NspI          302  ACATGC               [GA]CATG^[CT]
              603  GCATGC              
RsaI          591  GTAC                 GT^AC
              762  GTAC                
SacII         380  CCGCGG               CCGC^GG
              506  CCGCGG              
SfaNI         668  GCATC                GCATC(5/9)
              947  GCATC               
SmlI           10  CTCAAG               C^T[CT][GA]AG
             1005  CTCAAG              
TaqI          536  TCGA                 T^CGA
              632  TCGA                
TthHB8I       536  TCGA                 T^CGA
              632  TCGA                
XhoII         555  GGATCT               [GA]^GATC[CT]
              564  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AcyI        AluI        AvaI        AvaII       
BanI        BanII       BbvI        BcnI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsiYI       
BslI        BsmAI       BsmFI       BsoBI       Bsp1286I    
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PleI        
Sau3AI      Sau96I      ScrFI       StyI        TseI        
Tsp45I      
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