Restriction map for pF1KB4182
 (Linear) (Six-base) MAPSORT of: KIBB4182.seq  Check: 3808  from: 1  to: 1068



KIBB4182.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AatII G_ACGT'C

Cuts at:      0     662    1068 
   Size:        662     406
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619    1068 
   Size:        619     449
 
AhdI GACnn_n'nnGTC

Cuts at:      0     186    1068 
   Size:        186     882
 
ApaI G_GGCC'C

Cuts at:      0     644     993    1068 
   Size:        644     349      75

  Fragments arranged by size: 

                 644     349      75
 
ApaLI G'TGCA_C

Cuts at:      0      34    1068 
   Size:         34    1034
 
AvaI C'yCGr_G

Cuts at:      0      53     694     992    1068 
   Size:         53     641     298      76

  Fragments arranged by size: 

                 641     298      76      53
 
BanI G'GyrC_C

Cuts at:      0     391     730     739    1068 
   Size:        391     339       9     329

  Fragments arranged by size: 

                 391     339     329       9
 
BanII G_rGCy'C

Cuts at:      0     179     313     644     993    1068 
   Size:        179     134     331     349      75

  Fragments arranged by size: 

                 349     331     179     134      75
 
BbeI G_GCGC'C

Cuts at:      0     734    1068 
   Size:        734     334
 
BbvCI CC'TCA_GC

Cuts at:      0     664    1068 
   Size:        664     404
 
BclI T'GATC_A

Cuts at:      0      77    1068 
   Size:         77     991
 
BglI GCCn_nnn'nGGC

Cuts at:      0     772    1068 
   Size:        772     296
 
BglII A'GATC_T

Cuts at:      0     564    1068 
   Size:        564     504
 
BlpI GC'TnA_GC

Cuts at:      0     882    1068 
   Size:        882     186
 
Bme1580I G_kGCm'C

Cuts at:      0      38     394     475     491     581     644     993    1068 
   Size:         38     356      81      16      90      63     349      75

  Fragments arranged by size: 

                 356     349      90      81      75      63      38      16
            
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      79    1068 
   Size:         79     989
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     862     915     973    1068 
   Size:        862      53      58      95

  Fragments arranged by size: 

                 862      95      58      53
 
Bpu10I CC'TnA_GC

Cuts at:      0     664    1068 
   Size:        664     404
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     990    1068 
   Size:        990      78
 
BsaAI yAC'GTr

Cuts at:      0     590    1068 
   Size:        590     478
 
BsaHI Gr'CG_yC

Cuts at:      0     134     659     731    1068 
   Size:        134     525      72     337

  Fragments arranged by size: 

                 525     337     134      72
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     215     245     738     768     805     835     907     937 
   Size:        215      30     493      30      37      30      72      30

Cuts at:    937    1068 
   Size:        131

  Fragments arranged by size: 

                 493     215     131      72      37      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     478     493     523     697     712     970    1057    1060 
   Size:        478      15      30     174      15     258      87       3

Cuts at:   1060    1063    1068 
   Size:          3       5

  Fragments arranged by size: 

                 478     258     174      87      30      15      15       5
                   3       3
 
BseYI C'CCAG_C

Cuts at:      0      90     306     359     651     871    1068 
   Size:         90     216      53     292     220     197

  Fragments arranged by size: 

                 292     220     216     197      90      53
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     921    1068 
   Size:        921     147
 
BsiEI CG_ry'CG

Cuts at:      0      71    1068 
   Size:         71     997
 
BsiHKAI G_wGCw'C

Cuts at:      0      38    1068 
   Size:         38    1030
 
BsiWI C'GTAC_G

Cuts at:      0     590     761    1068 
   Size:        590     171     307

  Fragments arranged by size: 

                 590     307     171
 
BsmI GAATG_Cn'

Cuts at:      0     348    1068 
   Size:        348     720
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     125    1068 
   Size:        125     943
 
Bsp1286I G_dGCh'C

Cuts at:      0      38     179     313     394     475     491     581     644 
   Size:         38     141     134      81      81      16      90      63

Cuts at:    644     993    1068 
   Size:        349      75

  Fragments arranged by size: 

                 349     141     134      90      81      81      75      63
                  38      16
 
BsrBI CCG'CTC

Cuts at:      0      58     288    1068 
   Size:         58     230     780

  Fragments arranged by size: 

                 780     230      58
 
BstEII G'GTnAC_C

Cuts at:      0     273     712    1068 
   Size:        273     439     356

  Fragments arranged by size: 

                 439     356     273
 
BstYI r'GATC_y

Cuts at:      0     555     564    1068 
   Size:        555       9     504

  Fragments arranged by size: 

                 555     504       9
 
Bsu36I CC'TnA_GG

Cuts at:      0     942    1068 
   Size:        942     126
 
BtgI C'CryG_G

Cuts at:      0     380     506     717     767    1068 
   Size:        380     126     211      50     301

  Fragments arranged by size: 

                 380     301     211     126      50
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     473     737    1068 
   Size:        473     264     331

  Fragments arranged by size: 

                 473     331     264
 
BtsI GCAGTG_nn'

Cuts at:      0     491    1068 
   Size:        491     577
 
EaeI y'GGCC_r

Cuts at:      0      68     120    1068 
   Size:         68      52     948

  Fragments arranged by size: 

                 948      68      52
 
EagI C'GGCC_G

Cuts at:      0      68    1068 
   Size:         68    1000
 
EarI CTCTTCn'nnn_

Cuts at:      0     213    1068 
   Size:        213     855
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      91    1068 
   Size:         91     977
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619     862     915     973    1068 
   Size:        619     243      53      58      95

  Fragments arranged by size: 

                 619     243      95      58      53
 
EcoO109I rG'GnC_Cy

Cuts at:      0     640     641     690     989    1068 
   Size:        640       1      49     299      79

  Fragments arranged by size: 

                 640     299      79      49       1
 
HaeII r_GCGC'y

Cuts at:      0      48      86     734    1068 
   Size:         48      38     648     334

  Fragments arranged by size: 

                 648     334      48      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     172     204     805     837     907     939    1068 
   Size:        172      32     601      32      70      32     129

  Fragments arranged by size: 

                 601     172     129      70      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     730    1068 
   Size:        730     338
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     475    1068 
   Size:        475     593
 
MslI CAynn'nnrTG

Cuts at:      0     722    1068 
   Size:        722     346
 
MspA1I CmG'CkG

Cuts at:      0      90     382     508     736     865     935    1068 
   Size:         90     292     126     228     129      70     133

  Fragments arranged by size: 

                 292     228     133     129     126      90      70
 
NarI GG'CG_CC

Cuts at:      0     731    1068 
   Size:        731     337
 
NcoI C'CATG_G

Cuts at:      0     717    1068 
   Size:        717     351
 
NspI r_CATG'y

Cuts at:      0     306     607    1068 
   Size:        306     301     461

  Fragments arranged by size: 

                 461     306     301
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     840    1068 
   Size:        840     228
 
PfoI T'CCnGG_A

Cuts at:      0     238     950    1068 
   Size:        238     712     118

  Fragments arranged by size: 

                 712     238     118
 
PpuMI rG'GwC_Cy

Cuts at:      0     690    1068 
   Size:        690     378
 
PspOMI G'GGCC_C

Cuts at:      0     640     989    1068 
   Size:        640     349      79

  Fragments arranged by size: 

                 640     349      79
 
PvuII CAG'CTG

Cuts at:      0     935    1068 
   Size:        935     133
 
SacII CC_GC'GG

Cuts at:      0     383     509    1068 
   Size:        383     126     559

  Fragments arranged by size: 

                 559     383     126
 
SanDI GG'GwC_CC

Cuts at:      0     690    1068 
   Size:        690     378
 
SfoI GGC'GCC

Cuts at:      0     732    1068 
   Size:        732     336
 
SmaI CCC'GGG

Cuts at:      0     994    1068 
   Size:        994      74
 
SmlI C'TyrA_G

Cuts at:      0      10    1005    1068 
   Size:         10     995      63

  Fragments arranged by size: 

                 995      63      10
 
SphI G_CATG'C

Cuts at:      0     607    1068 
   Size:        607     461
 
SrfI GCCC'GGGC

Cuts at:      0     994    1068 
   Size:        994      74
 
StuI AGG'CCT

Cuts at:      0     547    1068 
   Size:        547     521
 
StyI C'CwwG_G

Cuts at:      0     191     581     717    1068 
   Size:        191     390     136     351

  Fragments arranged by size: 

                 390     351     191     136
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     643     763    1068 
   Size:        643     120     305

  Fragments arranged by size: 

                 643     305     120
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     738     770     838     870    1068 
   Size:        738      32      68      32     198

  Fragments arranged by size: 

                 738     198      68      32      32
 
XmaI C'CCGG_G

Cuts at:      0     992    1068 
   Size:        992      76
 
ZraI GAC'GTC

Cuts at:      0     660    1068 
   Size:        660     408
 

 Enzymes that do cut:

    AatII     AcuI     AhdI     ApaI    ApaLI     AvaI     BanI    BanII
     BbeI    BbvCI     BclI     BglI    BglII     BlpI Bme1580I     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BsrBI
   BstEII    BstYI   Bsu36I     BtgI    BtgZI     BtsI     EaeI     EagI
     EarI     EciI  Eco57MI EcoO109I    HaeII    Hin4I     KasI     MmeI
     MslI   MspA1I     NarI     NcoI     NspI    PflMI     PfoI    PpuMI
   PspOMI    PvuII    SacII    SanDI     SfoI     SmaI     SmlI     SphI
     SrfI     StuI     StyI    TaqII     TstI     XmaI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI     BcgI    BciVI    BfrBI    BmgBI
     BmrI     BmtI     BsaI    BsaBI    BsaWI    BspEI    BspHI    BspMI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
  BstZ17I     ClaI    CspCI     DraI   DraIII     DrdI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MscI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SspI     SwaI     TatI
  Tth111I     XbaI     XcmI     XhoI     XmnI
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