Restriction map for pF1KB4163
##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AgeI        AhdI        ApaI        ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvrII       BbvCI       BciVI       BclI        BfrI        
BlnI        BlpI        Bpu1102I    BsiWI       BsmI        
Bsp1407I    BspLU11I    BsrDI       BsrGI       BssHII      
BssSI       BstBI       CelII       CpoI        DraI        
DrdI        EagI        Eam1105I    EclXI       Eco52I      
EcoNI       EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KpnI        KspI        
MfeI        MluI        MunI        NaeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfuI        
SgrAI       SnaBI       SpeI        Sse8387I    SspBI       
SwaI        Tth111I     XbaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccIII        377  TCCGGA               T^CCGGA
AflIII       2783  ACGTGT               A^C[GA][CT]GT
AocI         1324  CCTGAGG              CC^T[ACGT]AGG
Asp700I       963  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI         1580  GGCGCC               GGCGC^C
BbrPI        2782  CACGTG               CAC^GTG
BbsI         2443  GTCTTC               GAAGAC(2/6)
BfaI          202  CTAG                 C^TAG
BpuAI        2443  GTCTTC               GAAGAC(2/6)
BsaBI         657  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         377  TCCGGA               [AT]^CCGG[AT]
BseAI         377  TCCGGA               T^CCGGA
BsiEI         699  CGACCG               CG[GA][CT]^CG
BsmBI        1651  GAGACG               CGTCTC(1/5)
BspEI         377  TCCGGA               T^CCGGA
BsrFI        2504  ACCGGC               [GA]^CCGG[CT]
Bst1107I      205  GTATAC               GTA^TAC
BstAPI       2587  GCAGAGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I       205  GTATAC               GTA^TAC
Bsu36I       1324  CCTGAGG              CC^T[ACGT]AGG
Cfr10I       2504  ACCGGC               [GA]^CCGG[CT]
Eco81I       1324  CCTGAGG              CC^T[ACGT]AGG
HgaI         1760  GCGTC                GACGC(5/10)
KasI         1580  GGCGCC               G^GCGCC
MaeI          202  CTAG                 C^TAG
MamI          657  GATCTTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          377  TCCGGA               T^CCGGA
MseI          494  TTAA                 T^TAA
NarI         1580  GGCGCC               GG^CGCC
PmaCI        2782  CACGTG               CAC^GTG
PmlI         2782  CACGTG               CAC^GTG
PshAI        1200  GACCAAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI           61  GAGCTC               GAGCT^C
SfoI         1580  GGCGCC               GGC^GCC
SmaI         1331  CCCGGG               CCC^GGG
SphI          170  GCATGC               GCATG^C
SspI           14  AATATT               AAT^ATT
TfiI         1302  GAATC                G^A[AT]TC
Tru9I         494  TTAA                 T^TAA
XmaCI        1331  CCCGGG               C^CCGGG
XmaI         1331  CCCGGG               C^CCGGG
XmnI          963  GAAGGTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AccI          205  GTATAC               GT^[AC][GT]AC
             1717  GTCTAC              
AcyI         1580  GGCGCC               G[GA]^CG[CT]C
             1759  GGCGTC              
Alw44I        639  GTGCAC               G^TGCAC
             1765  GTGCAC              
Aor51HI       546  AGCGCT               AGC^GCT
             1166  AGCGCT              
ApaLI         639  GTGCAC               G^TGCAC
             1765  GTGCAC              
AviII         410  TGCGCA               TGC^GCA
              908  TGCGCA              
BamHI         399  GGATCC               G^GATCC
             2717  GGATCC              
BsaAI         997  TACGTG               [CT]AC^GT[GA]
             2782  CACGTG              
BsaHI        1580  GGCGCC               G[GA]^CG[CT]C
             1759  GGCGTC              
BsaI         1492  GAGACC               GGTCTC(1/5)
             2800  GAGACC              
BseRI        1894  CTCCTC               GAGGAG(10/8)
             1944  CTCCTC              
BspDI         117  ATCGAT               AT^CGAT
             2437  ATCGAT              
BsrBI         724  GAGCGG               CCGCTC(-3/-3)
             1923  GAGCGG              
BstEII        729  GGTCACC              G^GT[ACGT]ACC
             1888  GGTGACC             
BstPI         729  GGTCACC              G^GT[ACGT]ACC
             1888  GGTGACC             
ClaI          117  ATCGAT               AT^CGAT
             2437  ATCGAT              
DraII         287  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1514  AGGTCCT             
EarI          148  CTCTTC               CTCTTC(1/4)
             1648  GAAGAG              
Eco47III      546  AGCGCT               AGC^GCT
             1166  AGCGCT              
EcoO109I      287  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1514  AGGTCCT             
EcoO65I       729  GGTCACC              G^GT[ACGT]ACC
             1888  GGTGACC             
FspI          410  TGCGCA               TGC^GCA
              908  TGCGCA              
Hin1I        1580  GGCGCC               G[GA]^CG[CT]C
             1759  GGCGTC              
Ksp632I       148  CTCTTC               CTCTTC(1/4)
             1648  GAAGAG              
NdeI          404  CATATG               CA^TATG
             2123  CATATG              
NspI          170  GCATGC               [GA]CATG^[CT]
             2660  ACATGC              
PaeR7I       1408  CTCGAG               C^TCGAG
             1794  CTCGAG              
PpuMI         287  AGGACCC              [GA]G^G[AT]CC[CT]
             1514  AGGTCCT             
SfiI          249  GGCCATTATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1035  GGCCTCTCAGGCC       
Tsp509I       223  AATT                 ^AATT
              558  AATT                
XhoI         1408  CTCGAG               C^TCGAG
             1794  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  102 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbvI        BcgI        BcnI        
BglI        BglII       BmyI        BpmI        BsaJI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspHI       BspMI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraIII      DsaI        EaeI        
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PflMI       PleI        PstI        PvuII       RcaI        
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StuI        StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XcmI        XhoII       
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