Restriction map for pF1KB4163
 (Linear) (Six-base) MAPSORT of: KIBB4163.seq  Check: 8880  from: 1  to: 2817



KIBB4163.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:57  ..

AccI GT'mk_AC

Cuts at:      0     206    1718    2817 
   Size:        206    1512    1099

  Fragments arranged by size: 

                1512    1099     206
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     214     409     970    1667    2436    2698    2773 
   Size:         73     141     195     561     697     769     262      75

Cuts at:   2773    2817 
   Size:         44

  Fragments arranged by size: 

                 769     697     561     262     195     141      75      73
                  44
 
AfeI AGC'GCT

Cuts at:      0     548    1168    2817 
   Size:        548     620    1649

  Fragments arranged by size: 

                1649     620     548
 
AflIII A'CryG_T

Cuts at:      0    2783    2817 
   Size:       2783      34
 
AleI CACnn'nnGTG

Cuts at:      0     271    2817 
   Size:        271    2546
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     444     601    2623    2716    2817 
   Size:        444     157    2022      93     101

  Fragments arranged by size: 

                2022     444     157     101      93
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     589     621    2817 
   Size:        589      32    2196

  Fragments arranged by size: 

                2196     589      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     424    1452    1503    2573    2593    2676    2817 
   Size:        424    1028      51    1070      20      83     141

  Fragments arranged by size: 

                1070    1028     424     141      83      51      20
 
ApaLI G'TGCA_C

Cuts at:      0     639    1765    2817 
   Size:        639    1126    1052

  Fragments arranged by size: 

                1126    1052     639
 
AvaI C'yCGr_G

Cuts at:      0    1331    1408    1794    2817 
   Size:       1331      77     386    1023

  Fragments arranged by size: 

                1331    1023     386      77
 
BamHI G'GATC_C

Cuts at:      0     399    2717    2817 
   Size:        399    2318     100

  Fragments arranged by size: 

                2318     399     100
 
BanI G'GyrC_C

Cuts at:      0    1580    2005    2019    2107    2185    2817 
   Size:       1580     425      14      88      78     632

  Fragments arranged by size: 

                1580     632     425      88      78      14
 
BanII G_rGCy'C

Cuts at:      0      65     312    1670    1816    2209    2383    2817 
   Size:         65     247    1358     146     393     174     434

  Fragments arranged by size: 

                1358     434     393     247     174     146      65
 
BbeI G_GCGC'C

Cuts at:      0    1584    2817 
   Size:       1584    1233
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2436    2817 
   Size:       2436     381
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     570     604    1167    1201    1995    2029    2817 
   Size:        570      34     563      34     794      34     788

  Fragments arranged by size: 

                 794     788     570     563      34      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     256    1042    2161    2204    2817 
   Size:        256     786    1119      43     613

  Fragments arranged by size: 

                1119     786     613     256      43
 
BglII A'GATC_T

Cuts at:      0      41     656    2177    2354    2817 
   Size:         41     615    1521     177     463

  Fragments arranged by size: 

                1521     615     463     177      41
 
Bme1580I G_kGCm'C

Cuts at:      0     147     643    1769    2008    2110    2817 
   Size:        147     496    1126     239     102     707

  Fragments arranged by size: 

                1126     707     496     239     147     102
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1727    1912    2550    2817 
   Size:       1727     185     638     267

  Fragments arranged by size: 

                1727     638     267     185
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208    1027    1417    1853    2194    2299    2398    2817 
   Size:        208     819     390     436     341     105      99     419

  Fragments arranged by size: 

                 819     436     419     390     341     208     105      99
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    2165    2817 
   Size:       2165     652
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     826    2794    2817 
   Size:        826    1968      23

  Fragments arranged by size: 

                1968     826      23
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1486    2794    2817 
   Size:       1486    1308      23

  Fragments arranged by size: 

                1486    1308      23
 
BsaAI yAC'GTr

Cuts at:      0     999    2784    2817 
   Size:        999    1785      33

  Fragments arranged by size: 

                1785     999      33
 
BsaBI GATnn'nnATC

Cuts at:      0     661    2817 
   Size:        661    2156
 
BsaHI Gr'CG_yC

Cuts at:      0    1581    1760    2817 
   Size:       1581     179    1057

  Fragments arranged by size: 

                1581    1057     179
 
BsaWI w'CCGG_w

Cuts at:      0     377    2817 
   Size:        377    2440
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     490     520     793     823    1606    1636    2817 
   Size:        490      30     273      30     783      30    1181

  Fragments arranged by size: 

                1181     783     490     273      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1885    1935    2817 
   Size:       1885      50     882

  Fragments arranged by size: 

                1885     882      50
 
BseYI C'CCAG_C

Cuts at:      0     458     574    1884    2151    2734    2777    2817 
   Size:        458     116    1310     267     583      43      40

  Fragments arranged by size: 

                1310     583     458     267     116      43      40
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     949    1528    1972    2455    2724    2817 
   Size:        949     579     444     483     269      93

  Fragments arranged by size: 

                 949     579     483     444     269      93
 
BsiEI CG_ry'CG

Cuts at:      0     702    2817 
   Size:        702    2115
 
BsiHKAI G_wGCw'C

Cuts at:      0      65     643     770    1180    1432    1769    1839    2058 
   Size:         65     578     127     410     252     337      70     219

Cuts at:   2058    2766    2817 
   Size:        708      51

  Fragments arranged by size: 

                 708     578     410     337     252     219     127      70
                  65      51
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1645    2817 
   Size:       1645    1172
 
Bsp1286I G_dGCh'C

Cuts at:      0      65     147     312     643     770    1180    1432    1670 
   Size:         65      82     165     331     127     410     252     238

Cuts at:   1670    1769    1816    1839    2008    2058    2110    2209    2383 
   Size:         99      47      23     169      50      52      99     174

Cuts at:   2383    2766    2817 
   Size:        383      51

  Fragments arranged by size: 

                 410     383     331     252     238     174     169     165
                 127      99      99      82      65      52      51      50
                  47      23
 
BspEI T'CCGG_A

Cuts at:      0     377    2817 
   Size:        377    2440
 
BspHI T'CATG_A

Cuts at:      0    1022    2252    2387    2817 
   Size:       1022    1230     135     430

  Fragments arranged by size: 

                1230    1022     430     135
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     593    1503    1562    1889    2231    2238    2817 
   Size:        593     910      59     327     342       7     579

  Fragments arranged by size: 

                 910     593     579     342     327      59       7
 
BsrBI CCG'CTC

Cuts at:      0     726    1925    2817 
   Size:        726    1199     892

  Fragments arranged by size: 

                1199     892     726
 
BsrFI r'CCGG_y

Cuts at:      0    2504    2817 
   Size:       2504     313
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2593    2817 
   Size:       2593     224
 
BstEII G'GTnAC_C

Cuts at:      0     729    1888    2817 
   Size:        729    1159     929

  Fragments arranged by size: 

                1159     929     729
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     528     540    1149    1617    2016    2103    2217    2817 
   Size:        528      12     609     468     399      87     114     600

  Fragments arranged by size: 

                 609     600     528     468     399     114      87      12
            
 
BstYI r'GATC_y

Cuts at:      0      41     399     656     944    2177    2354    2717    2817 
   Size:         41     358     257     288    1233     177     363     100

  Fragments arranged by size: 

                1233     363     358     288     257     177     100      41
            
 
BstZ17I GTA'TAC

Cuts at:      0     207    2817 
   Size:        207    2610
 
Bsu36I CC'TnA_GG

Cuts at:      0    1325    2817 
   Size:       1325    1492
 
BtgI C'CryG_G

Cuts at:      0     125     326     521    1563    1592    2216    2817 
   Size:        125     201     195    1042      29     624     601

  Fragments arranged by size: 

                1042     624     601     201     195     125      29
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1035    2817 
   Size:       1035    1782
 
BtsI GCAGTG_nn'

Cuts at:      0     104     323     545     835     868    2073    2467    2817 
   Size:        104     219     222     290      33    1205     394     350

  Fragments arranged by size: 

                1205     394     350     290     222     219     104      33
            
 
ClaI AT'CG_AT

Cuts at:      0     118    2438    2817 
   Size:        118    2320     379

  Fragments arranged by size: 

                2320     379     118
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1073    1108    2817 
   Size:       1073      35    1709

  Fragments arranged by size: 

                1709    1073      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     647    1295    1297    2817 
   Size:        647     648       2    1520

  Fragments arranged by size: 

                1520     648     647       2
 
EaeI y'GGCC_r

Cuts at:      0     248     257     434     530    1131    1526    1937    2420 
   Size:        248       9     177      96     601     395     411     483

Cuts at:   2420    2519    2817 
   Size:         99     298

  Fragments arranged by size: 

                 601     483     411     395     298     248     177      99
                  96       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     154    1643    2817 
   Size:        154    1489    1174

  Fragments arranged by size: 

                1489    1174     154
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1018    2722    2817 
   Size:       1018    1704      95

  Fragments arranged by size: 

                1704    1018      95
 
EcoICRI GAG'CTC

Cuts at:      0      63    2817 
   Size:         63    2754
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     208     214     409     970    1027    1417    1667 
   Size:         73     135       6     195     561      57     390     250

Cuts at:   1667    1853    2194    2299    2398    2436    2698    2773    2817 
   Size:        186     341     105      99      38     262      75      44

  Fragments arranged by size: 

                 561     390     341     262     250     195     186     135
                 105      99      75      73      57      44      38       6
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0     288    1515    2817 
   Size:        288    1227    1302

  Fragments arranged by size: 

                1302    1227     288
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     987    1266    2817 
   Size:        987     279    1551

  Fragments arranged by size: 

                1551     987     279
 
FspI TGC'GCA

Cuts at:      0     412     910    2817 
   Size:        412     498    1907

  Fragments arranged by size: 

                1907     498     412
 
HaeII r_GCGC'y

Cuts at:      0     550    1170    1584    2817 
   Size:        550     620     414    1233

  Fragments arranged by size: 

                1233     620     550     414
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     490     522     793     825    2045    2077    2385    2417 
   Size:        490      32     271      32    1220      32     308      32

Cuts at:   2417    2817 
   Size:        400

  Fragments arranged by size: 

                1220     490     400     308     271      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     165     264     345    1209    2817 
   Size:        165      99      81     864    1608

  Fragments arranged by size: 

                1608     864     165      99      81
 
KasI G'GCGC_C

Cuts at:      0    1580    2817 
   Size:       1580    1237
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     533    2050    2817 
   Size:        533    1517     767

  Fragments arranged by size: 

                1517     767     533
 
MscI TGG'CCA

Cuts at:      0     250     436     532    1528    1939    2521    2817 
   Size:        250     186      96     996     411     582     296

  Fragments arranged by size: 

                 996     582     411     296     250     186      96
 
MslI CAynn'nnrTG

Cuts at:      0     271     526     802    1147    2215    2392    2698    2817 
   Size:        271     255     276     345    1068     177     306     119

  Fragments arranged by size: 

                1068     345     306     276     271     255     177     119
            
 
MspA1I CmG'CkG

Cuts at:      0     424     462     760    1136    1443    1449    2229    2738 
   Size:        424      38     298     376     307       6     780     509

Cuts at:   2738    2817 
   Size:         79

  Fragments arranged by size: 

                 780     509     424     376     307     298      79      38
                   6
 
NarI GG'CG_CC

Cuts at:      0    1581    2817 
   Size:       1581    1236
 
NcoI C'CATG_G

Cuts at:      0     326     521    2216    2817 
   Size:        326     195    1695     601

  Fragments arranged by size: 

                1695     601     326     195
 
NdeI CA'TA_TG

Cuts at:      0     405    2124    2817 
   Size:        405    1719     693

  Fragments arranged by size: 

                1719     693     405
 
NspI r_CATG'y

Cuts at:      0     174    2664    2817 
   Size:        174    2490     153

  Fragments arranged by size: 

                2490     174     153
 
PasI CC'CwG_GG

Cuts at:      0    1872    2817 
   Size:       1872     945
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     465    1062    1346    1503    2349    2817 
   Size:        465     597     284     157     846     468

  Fragments arranged by size: 

                 846     597     468     465     284     157
 
PfoI T'CCnGG_A

Cuts at:      0     482     665    1220    2817 
   Size:        482     183     555    1597

  Fragments arranged by size: 

                1597     555     482     183
 
PmlI CAC'GTG

Cuts at:      0    2784    2817 
   Size:       2784      33
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2542    2574    2817 
   Size:       2542      32     243

  Fragments arranged by size: 

                2542     243      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     288    1515    2817 
   Size:        288    1227    1302

  Fragments arranged by size: 

                1302    1227     288
 
PshAI GACnn'nnGTC

Cuts at:      0    1204    2817 
   Size:       1204    1613
 
PspXI vC'TCGA_Gb

Cuts at:      0    1408    1794    2817 
   Size:       1408     386    1023

  Fragments arranged by size: 

                1408    1023     386
 
PstI C_TGCA'G

Cuts at:      0     896    1448    2228    2612    2817 
   Size:        896     552     780     384     205

  Fragments arranged by size: 

                 896     780     552     384     205
 
PvuII CAG'CTG

Cuts at:      0     424     462    1443    1449    2229    2738    2817 
   Size:        424      38     981       6     780     509      79

  Fragments arranged by size: 

                 981     780     509     424      79      38       6
 
SacI G_AGCT'C

Cuts at:      0      65    2817 
   Size:         65    2752
 
SfcI C'TryA_G

Cuts at:      0     892    1444    2224    2608    2817 
   Size:        892     552     780     384     209

  Fragments arranged by size: 

                 892     780     552     384     209
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     256    1042    2817 
   Size:        256     786    1775

  Fragments arranged by size: 

                1775     786     256
 
SfoI GGC'GCC

Cuts at:      0    1582    2817 
   Size:       1582    1235
 
SmaI CCC'GGG

Cuts at:      0    1333    2817 
   Size:       1333    1484
 
SmlI C'TyrA_G

Cuts at:      0     841    1408    1794    2809    2817 
   Size:        841     567     386    1015       8

  Fragments arranged by size: 

                1015     841     567     386       8
 
SphI G_CATG'C

Cuts at:      0     174    2817 
   Size:        174    2643
 
SrfI GCCC'GGGC

Cuts at:      0    1333    2817 
   Size:       1333    1484
 
SspI AAT'ATT

Cuts at:      0      16    2817 
   Size:         16    2801
 
StuI AGG'CCT

Cuts at:      0     939    2242    2794    2817 
   Size:        939    1303     552      23

  Fragments arranged by size: 

                1303     939     552      23
 
StyI C'CwwG_G

Cuts at:      0     326     521     692    1202    1610    2216    2817 
   Size:        326     195     171     510     408     606     601

  Fragments arranged by size: 

                 606     601     510     408     326     195     171
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1876    2073    2272    2373    2688    2817 
   Size:       1876     197     199     101     315     129

  Fragments arranged by size: 

                1876     315     199     197     129     101
 
TatI w'GTAC_w

Cuts at:      0     980    2817 
   Size:        980    1837
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2111    2143    2459    2491    2817 
   Size:       2111      32     316      32     326

  Fragments arranged by size: 

                2111     326     316      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     759    1599    1740    2346    2643    2817 
   Size:        759     840     141     606     297     174

  Fragments arranged by size: 

                 840     759     606     297     174     141
 
XhoI C'TCGA_G

Cuts at:      0    1408    1794    2817 
   Size:       1408     386    1023

  Fragments arranged by size: 

                1408    1023     386
 
XmaI C'CCGG_G

Cuts at:      0    1331    2817 
   Size:       1331    1486
 
XmnI GAAnn'nnTTC

Cuts at:      0     967    2817 
   Size:        967    1850
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AleI     AlfI     AloI    AlwNI
    ApaLI     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI
     BglI    BglII Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrFI   BstAPI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I     FalI     FspI    HaeII    Hin4I   HincII
     KasI     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NdeI
     NspI     PasI    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI
    PspXI     PstI    PvuII     SacI     SfcI     SfiI     SfoI     SmaI
     SmlI     SphI     SrfI     SspI     StuI     StyI    TaqII     TatI
     TstI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AhdI     ApaI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI    BciVI
     BclI    BfrBI     BlpI    BmgBI     BmtI     BplI    BsiWI     BsmI
    BsrDI    BsrGI   BssHII    BssSI    BstBI     DraI     DrdI     EagI
    EcoNI    EcoRI    EcoRV     FseI    FspAI  HindIII     HpaI     KpnI
     MfeI     MluI     NaeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PsiI   PspOMI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SgfI
    SgrAI    SnaBI     SpeI     SwaI  Tth111I     XbaI     ZraI
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