Restriction map for pF1KB4046
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflIII      AgeI        AocI        ApaI        ApoI        
AscI        AseI        AsnI        Asp718I     AspI        
AviII       AvrII       BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BlnI        BsaAI       
BsaI        BseAI       BsiWI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrGI       BstBI       
BstEII      BstPI       Bsu36I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        MfeI        
MluI        MroI        MunI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SbfI        SexAI       SfiI        
SfuI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tsp509I     Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   64 enzymes) #
###################################

AfaI          660  GTAC                 GT^AC
AflII        1219  CTTAAG               C^TTAAG
AhdI         1216  GACCTTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       1015  GTGCAC               G^TGCAC
AlwI         1321  GATCC                GGATC(4/5)
AlwNI          95  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       121  AGCGCT               AGC^GCT
ApaLI        1015  GTGCAC               G^TGCAC
Asp700I       157  GAAGTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1216  GACCTTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          152  CCCGGG               C^[CT]CG[GA]G
BalI          847  TGGCCA               TGG^CCA
BbeI         1293  GGCGCC               GGCGC^C
BbsI          586  GTCTTC               GAAGAC(2/6)
BfaI         1325  CTAG                 C^TAG
BfrI         1219  CTTAAG               C^TTAAG
BglI          840  GCCCAAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         920  AGATCT               A^GATCT
BpuAI         586  GTCTTC               GAAGAC(2/6)
BsaBI         315  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1130  ACCGGA               [AT]^CCGG[AT]
BsgI         1156  GTGCAG               GTGCAG(16/14)
BsmI           60  GAATGC               GAATGC(1/-1)
BsoBI         152  CCCGGG               C^[CT]CG[GA]G
BsrBI         196  GAGCGG               CCGCTC(-3/-3)
BsrDI         507  CATTGC               GCAATG(2/0)
BsrFI         306  GCCGGC               [GA]^CCGG[CT]
BssHII        556  GCGCGC               G^CGCGC
BssSI         433  CACGAG               CACGAG(-5/-1)
Bst1107I     1161  GTATAC               GTA^TAC
BstAPI       1008  GCAGTTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         920  AGATCT               [GA]^GATC[CT]
BstZ17I      1161  GTATAC               GTA^TAC
Cfr10I        306  GCCGGC               [GA]^CCGG[CT]
Eam1105I     1216  GACCTTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      121  AGCGCT               AGC^GCT
EcoT14I      1193  CCAAGG               C^C[AT][AT]GG
KasI         1293  GGCGCC               G^GCGCC
MaeI         1325  CTAG                 C^TAG
MaeII        1306  ACGT                 A^CGT
MamI          315  GATTCCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          920  AGATCT               [GA]^GATC[CT]
MluNI         847  TGGCCA               TGG^CCA
MscI          847  TGGCCA               TGG^CCA
MseI         1220  TTAA                 T^TAA
NaeI          306  GCCGGC               GCC^GGC
NarI         1293  GGCGCC               GG^CGCC
NgoMIV        306  GCCGGC               G^CCGGC
NlaIII       1209  CATG                 CATG^
PflMI          94  CCAGCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI          660  GTAC                 GT^AC
SapI          399  GCTCTTC              GCTCTTC(1/4)
ScaI          659  AGTACT               AGT^ACT
SfoI         1293  GGCGCC               GGC^GCC
SgrAI         305  CGCCGGCG             C[GA]^CCGG[CT]G
SmaI          152  CCCGGG               CCC^GGG
StyI         1193  CCAAGG               C^C[AT][AT]GG
Tru9I        1220  TTAA                 T^TAA
Van91I         94  CCAGCACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          850  CCAAAGGCCTTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         920  AGATCT               [GA]^GATC[CT]
XmaCI         152  CCCGGG               C^CCGGG
XmaI          152  CCCGGG               C^CCGGG
XmnI          157  GAAGTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI         1084  GTCTAC               GT^[AC][GT]AC
             1161  GTATAC              
BlpI          394  GCTGAGC              GC^T[ACGT]AGC
              829  GCTGAGC             
Bpu1102I      394  GCTGAGC              GC^T[ACGT]AGC
              829  GCTGAGC             
BseRI          73  CTCCTC               GAGGAG(10/8)
              436  GAGGAG              
BsiEI         611  CGGCCG               CG[GA][CT]^CG
              880  CGGCCG              
BsmAI         439  GAGAC                GTCTC(1/5)
              712  GTCTC               
BsmBI         711  CGTCTC               CGTCTC(1/5)
              439  GAGACG              
BsmFI         863  GGGAC                GGGAC(10/14)
             1172  GGGAC               
BspMI        1043  ACCTGC               ACCTGC(4/8)
             1104  GCAGGT              
BsrI          869  ACTGG                ACTGG(1/-1)
             1266  CCAGT               
BstXI        1193  CCAAGGCTCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1300  CCACTGACGTGG        
CelII         394  GCTGAGC              GC^T[ACGT]AGC
              829  GCTGAGC             
EagI          611  CGGCCG               C^GGCCG
              880  CGGCCG              
EarI           76  CTCTTC               CTCTTC(1/4)
              400  CTCTTC              
EclXI         611  CGGCCG               C^GGCCG
              880  CGGCCG              
Eco52I        611  CGGCCG               C^GGCCG
              880  CGGCCG              
HinfI         162  GATTC                G^A[ACGT]TC
              315  GATTC               
Ksp632I        76  CTCTTC               CTCTTC(1/4)
              400  CTCTTC              
KspI          263  CCGCGG               CCGC^GG
              608  CCGCGG              
MslI          507  CATTGCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1301  CACTGACGTG          
PvuII        1027  CAGCTG               CAG^CTG
             1099  CAGCTG              
SacI          397  GAGCTC               GAGCT^C
              820  GAGCTC              
SacII         263  CCGCGG               CCGC^GG
              608  CCGCGG              
SfaNI         229  GATGC                GCATC(5/9)
              873  GATGC               
TfiI          162  GATTC                G^A[AT]TC
              315  GATTC               
TspRI         348  CACTG                CA[GC]TG[ACGT][ACGT]^
             1301  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccII       AciI        AcyI        AluI        AspHI       
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaHI       BsaJI       BsiHKAI     
BsiYI       BslI        Bsp1286I    BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EaeI        EcoRII      
FauI        Fnu4HI      FokI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIV       
PstI        Sau3AI      Sau96I      ScrFI       SfcI        
SmlI        StuI        TaqI        TseI        Tsp45I      
TthHB8I     
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