Restriction map for pF1KB3955
##################################
# NO CUT ENZYMES (  143 enzymes) #
##################################

AccII       AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfaI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsoBI       BspDI       BspEI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
FauI        FseI        FspI        HaeII       HhaI        
HinP1I      HincII      HindII      HpaI        KasI        
KspI        MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MunI        
MvnI        NaeI        NarI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
PvuII       RsrII       SacI        SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII         620  GACGTC               GACGT^C
Acc65I        186  GGTACC               G^GTACC
AccI         1162  GTCTAC               GT^[AC][GT]AC
AcyI          620  GACGTC               G[GA]^CG[CT]C
AflIII       1355  ACGTGT               A^C[GA][CT]GT
ApaI          819  GGGCCC               GGGCC^C
Asp700I       133  GAACTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       186  GGTACC               G^GTACC
BanI          186  GGTACC               G^G[CT][GA]CC
BanII         819  GGGCCC               G[GA]GC[CT]^C
BbsI         1233  GAAGAC               GAAGAC(2/6)
BclI          372  TGATCA               T^GATCA
BcnI          486  CCGGG                CC^[GC]GG
BmyI          819  GGGCCC               G[AGT]GC[ACT]^C
BpmI         1148  CTGGAG               CTGGAG(16/14)
BpuAI        1233  GAAGAC               GAAGAC(2/6)
BsaAI        1354  TACGTG               [CT]AC^GT[GA]
BsaHI         620  GACGTC               G[GA]^CG[CT]C
BsmAI         872  GAGAC                GTCTC(1/5)
BsmI         1058  GCATTC               GAATGC(1/-1)
Bsp1286I      819  GGGCCC               G[AGT]GC[ACT]^C
Bsp1407I      353  TGTACA               T^GTACA
BspHI         120  TCATGA               T^CATGA
BsrGI         353  TGTACA               T^GTACA
BstEII         36  GGTCACC              G^GT[ACGT]ACC
BstPI          36  GGTCACC              G^GT[ACGT]ACC
EaeI          734  CGGCCA               [CT]^GGCC[GA]
EcoO65I        36  GGTCACC              G^GT[ACGT]ACC
EcoT22I       449  ATGCAT               ATGCA^T
FbaI          372  TGATCA               T^GATCA
HgaI         1221  GCGTC                GACGC(5/10)
Hin1I         620  GACGTC               G[GA]^CG[CT]C
HindIII       744  AAGCTT               A^AGCTT
KpnI          186  GGTACC               GGTAC^C
NciI          486  CCGGG                CC^[GC]GG
NdeI          791  CATATG               CA^TATG
NsiI          449  ATGCAT               ATGCA^T
NspI         1307  ACATGC               [GA]CATG^[CT]
PleI          796  GACTC                GAGTC(4/5)
PpuMI         906  AGGACCC              [GA]G^G[AT]CC[CT]
PstI          306  CTGCAG               CTGCA^G
RcaI          120  TCATGA               T^CATGA
SapI          958  GCTCTTC              GCTCTTC(1/4)
SfcI          306  CTGCAG               C^T[GA][CT]AG
SspBI         353  TGTACA               T^GTACA
TfiI          124  GATTC                G^A[AT]TC
XmnI          133  GAACTCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI         1180  GATCC                GGATC(4/5)
              928  GGATC               
AvaII         546  GGACC                G^G[AT]CC
              907  GGACC               
BglII         111  AGATCT               A^GATCT
              144  AGATCT              
BseRI         755  CTCCTC               GAGGAG(10/8)
             1261  GAGGAG              
BsmFI         435  GTCCC                GGGAC(10/14)
              853  GTCCC               
BstYI         111  AGATCT               [GA]^GATC[CT]
              144  AGATCT              
DraI          325  TTTAAA               TTT^AAA
             1018  TTTAAA              
EarI          959  CTCTTC               CTCTTC(1/4)
             1036  GAAGAG              
HapII         486  CCGG                 C^CGG
             1312  CCGG                
HinfI         124  GATTC                G^A[ACGT]TC
              796  GACTC               
HpaII         486  CCGG                 C^CGG
             1312  CCGG                
HphI           38  TCACC                GGTGA(8/7)
              501  TCACC               
Ksp632I       959  CTCTTC               CTCTTC(1/4)
             1036  GAAGAG              
MflI          111  AGATCT               [GA]^GATC[CT]
              144  AGATCT              
MslI          426  CACGCCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1285  CACTTTGATG          
MspI          486  CCGG                 C^CGG
             1312  CCGG                
TaqI          866  TCGA                 T^CGA
             1005  TCGA                
Tsp45I         37  GTCAC                ^GT[GC]AC
             1250  GTCAC               
TthHB8I       866  TCGA                 T^CGA
             1005  TCGA                
XhoII         111  AGATCT               [GA]^GATC[CT]
              144  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
BbvI        BsaJI       BsiYI       BslI        BsrI        
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       DsaI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MvaI        MwoI        NcoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        Tru9I       
TseI        Tsp509I     TspRI       
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