Restriction map for
pF1KB3758
(Circular) (Six-base) MAPSORT of: pF1KB3758.seq Check: 5852 from: 1 to: 4895
pF1KB3758 4895 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1461 1461
Size: 4895
AatII G_ACGT'C
Cuts at: 629 629
Size: 4895
AccI GT'mk_AC
Cuts at: 1933 1933
Size: 4895
Acc65I G'GTAC_C
Cuts at: 1911 1911
Size: 4895
AclI AA'CG_TT
Cuts at: 4735 4735
Size: 4895
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1303 2720 3152 3899 4167 1303
Size: 1417 432 747 268 2031
Fragments arranged by size:
2031 1417 747 432 268
AfeI AGC'GCT
Cuts at: 1297 1297
Size: 4895
AflIII A'CryG_T
Cuts at: 2247 3351 4328 2247
Size: 1104 977 2814
Fragments arranged by size:
2814 1104 977
AgeI A'CCGG_T
Cuts at: 3283 3283
Size: 4895
AlwNI CAG_nnn'CTG
Cuts at: 747 883 1281 1381 1767 2200 3767 747
Size: 136 398 100 386 433 1567 1875
Fragments arranged by size:
1875 1567 433 398 386 136 100
ApaI G_GGCC'C
Cuts at: 1732 1848 1732
Size: 116 4779
ApaLI G'TGCA_C
Cuts at: 182 3665 182
Size: 3483 1412
ApoI r'AATT_y
Cuts at: 1899 4248 4504 1899
Size: 2349 256 2290
Fragments arranged by size:
2349 2290 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4895
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4895
AvaI C'yCGr_G
Cuts at: 114 157 263 452 526 1915 114
Size: 43 106 189 74 1389 3094
Fragments arranged by size:
3094 1389 189 106 74 43
BamHI G'GATC_C
Cuts at: 259 1920 259
Size: 1661 3234
BanI G'GyrC_C
Cuts at: 350 1557 1590 1911 2325 2573 2608 350
Size: 1207 33 321 414 248 35 2637
Fragments arranged by size:
2637 1207 414 321 248 35 33
BanII G_rGCy'C
Cuts at: 218 312 348 808 863 1349 1732 1848 1909
Size: 94 36 460 55 486 383 116 61
Cuts at: 1909 2939 218
Size: 1030 2174
Fragments arranged by size:
2174 1030 486 460 383 116 94 61
55 36
BbeI G_GCGC'C
Cuts at: 354 2329 2577 354
Size: 1975 248 2672
Fragments arranged by size:
2672 1975 248
BbsI GAAGACnn'nnnn_
Cuts at: 502 629 971 502
Size: 127 342 4426
Fragments arranged by size:
4426 342 127
BbvCI CC'TCA_GC
Cuts at: 195 1069 1340 1490 1598 1804 195
Size: 874 271 150 108 206 3286
Fragments arranged by size:
3286 874 271 206 150 108
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4861
BciVI GTATCCnnnnn_n'
Cuts at: 2793 3554 4895 2793
Size: 761 1341 2793
Fragments arranged by size:
2793 1341 761
BclI T'GATC_A
Cuts at: 2415 2415
Size: 4895
BglI GCCn_nnn'nGGC
Cuts at: 325 1714 325
Size: 1389 3506
BglII A'GATC_T
Cuts at: 2410 4447 2410
Size: 2037 2858
BlpI GC'TnA_GC
Cuts at: 807 1199 2007 807
Size: 392 808 3695
Fragments arranged by size:
3695 808 392
Bme1580I G_kGCm'C
Cuts at: 186 244 1562 1732 1837 1848 2520 2613 3669
Size: 58 1318 170 105 11 672 93 1056
Cuts at: 3669 186
Size: 1412
Fragments arranged by size:
1412 1318 1056 672 170 105 93 58
11
BmrI ACTGGGnnnn_n'
Cuts at: 276 2107 2188 2523 4667 276
Size: 1831 81 335 2144 504
Fragments arranged by size:
2144 1831 504 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3262 3262
Size: 4895
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 589 670 850 1099 1177 1687 1732 1744
Size: 239 81 180 249 78 510 45 12
Cuts at: 1744 1804 350
Size: 60 3441
Fragments arranged by size:
3441 510 249 239 180 81 78 60
45 12
Bpu10I CC'TnA_GC
Cuts at: 195 542 998 1069 1222 1340 1490 1598 1804
Size: 347 456 71 153 118 150 108 206
Cuts at: 1804 195
Size: 3286
Fragments arranged by size:
3286 456 347 206 153 150 118 108
71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1150 2071 2950 3442 3740 1150
Size: 921 879 492 298 2305
Fragments arranged by size:
2305 921 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1633 4599 1633
Size: 2966 1929
BsaAI yAC'GTr
Cuts at: 2248 2248
Size: 4895
BsaBI GATnn'nnATC
Cuts at: 6 2409 4446 6
Size: 2403 2037 455
Fragments arranged by size:
2403 2037 455
BsaHI Gr'CG_yC
Cuts at: 351 499 626 2326 2574 3276 4772 351
Size: 148 127 1700 248 702 1496 474
Fragments arranged by size:
1700 1496 702 474 248 148 127
BsaWI w'CCGG_w
Cuts at: 210 304 386 2084 2605 3283 3557 3704 4115
Size: 94 82 1698 521 678 274 147 411
Cuts at: 4115 210
Size: 990
Fragments arranged by size:
1698 990 678 521 411 274 147 94
82
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 132 667 790 832 853 904 925 928 1096
Size: 535 123 42 21 51 21 3 168
Cuts at: 1096 1099 1357 1564 1582 1651 1669 132
Size: 3 258 207 18 69 18 3358
Fragments arranged by size:
3358 535 258 207 168 123 69 51
42 21 21 18 18 3 3
BseYI C'CCAG_C
Cuts at: 165 801 1572 1649 2320 3078 3655 4151 165
Size: 636 771 77 671 758 577 496 909
Fragments arranged by size:
909 771 758 671 636 577 496 77
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1009 1009
Size: 4895
BsiEI CG_ry'CG
Cuts at: 104 2437 2483 3691 104
Size: 2333 46 1208 1308
Fragments arranged by size:
2333 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 186 312 863 981 1223 1349 1909 2687 2877
Size: 126 551 118 242 126 560 778 190
Cuts at: 2877 3669 186
Size: 792 1412
Fragments arranged by size:
1412 792 778 560 551 242 190 126
126 118
BsmI GAATG_Cn'
Cuts at: 361 361
Size: 4895
BsmBI CGTCTCn'nnnn_
Cuts at: 1324 1361 1324
Size: 37 4858
Bsp1286I G_dGCh'C
Cuts at: 186 218 244 312 348 808 863 981 1223
Size: 32 26 68 36 460 55 118 242
Cuts at: 1223 1349 1562 1732 1837 1848 1909 2520 2613
Size: 126 213 170 105 11 61 611 93
Cuts at: 2613 2687 2877 2939 3669 186
Size: 74 190 62 730 1412
Fragments arranged by size:
1412 730 611 460 242 213 190 170
126 118 105 93 74 68 62 61
55 36 32 26 11
BspEI T'CCGG_A
Cuts at: 210 4115 210
Size: 3905 990
BspHI T'CATG_A
Cuts at: 4071 4071
Size: 4895
BspMI ACCTGCnnnn'nnnn_
Cuts at: 977 1461 1945 2159 2461 2842 977
Size: 484 484 214 302 381 3030
Fragments arranged by size:
3030 484 484 381 302 214
BsrBI CCG'CTC
Cuts at: 50 208 3187 3241 4725 4890 50
Size: 158 2979 54 1484 165 55
Fragments arranged by size:
2979 1484 165 158 55 54
BsrDI GCAATG_nn'
Cuts at: 1018 2807 1018
Size: 1789 3106
BsrFI r'CCGG_y
Cuts at: 128 146 518 1593 2893 3074 3283 128
Size: 18 372 1075 1300 181 209 1740
Fragments arranged by size:
1740 1300 1075 372 209 181 18
BssHII G'CGCG_C
Cuts at: 546 546
Size: 4895
BssSI C'ACGA_G
Cuts at: 3166 3524 3166
Size: 358 4537
BstAPI GCAn_nnn'nTGC
Cuts at: 2272 2272
Size: 4895
BstBI TT'CG_AA
Cuts at: 3256 3256
Size: 4895
BstXI CCAn_nnnn'nTGG
Cuts at: 671 1725 671
Size: 1054 3841
BstYI r'GATC_y
Cuts at: 1 259 1920 2387 2410 2745 2991 4003 4089
Size: 258 1661 467 23 335 246 1012 86
Cuts at: 4089 4101 4447 1
Size: 12 346 449
Fragments arranged by size:
1661 1012 467 449 346 335 258 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1435 1435
Size: 4895
BtgI C'CryG_G
Cuts at: 415 1839 415
Size: 1424 3471
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2845 3026 3189 2845
Size: 181 163 4551
Fragments arranged by size:
4551 181 163
BtsI GCAGTG_nn'
Cuts at: 722 979 1035 2138 722
Size: 257 56 1103 3479
Fragments arranged by size:
3479 1103 257 56
DraI TTT'AAA
Cuts at: 1894 1894
Size: 4895
DraIII CAC_nnn'GTG
Cuts at: 843 843
Size: 4895
DrdI GACnn_nn'nnGTC
Cuts at: 2601 3459 2601
Size: 858 4037
EaeI y'GGCC_r
Cuts at: 368 462 516 738 1769 2480 2654 3045 3072
Size: 94 54 222 1031 711 174 391 27
Cuts at: 3072 4417 368
Size: 1345 846
Fragments arranged by size:
1345 1031 846 711 391 222 174 94
54 27
EagI C'GGCC_G
Cuts at: 2480 2480
Size: 4895
EarI CTCTTCn'nnn_
Cuts at: 902 929 1100 1319 1610 2918 3128 902
Size: 27 171 219 291 1308 210 2669
Fragments arranged by size:
2669 1308 291 219 210 171 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 587 3103 3413 3559 4705 587
Size: 2516 310 146 1146 777
Fragments arranged by size:
2516 1146 777 310 146
EcoICRI GAG'CTC
Cuts at: 310 861 1347 1907 310
Size: 551 486 560 3298
Fragments arranged by size:
3298 560 551 486
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 350 589 670 850 1099 1177 1303 1687 1732
Size: 239 81 180 249 78 126 384 45
Cuts at: 1732 1744 1804 2720 3152 3899 4167 350
Size: 12 60 916 432 747 268 1078
Fragments arranged by size:
1078 916 747 432 384 268 249 239
180 126 81 78 60 45 12
EcoO109I rG'GnC_Cy
Cuts at: 162 714 1729 1844 1845 2034 162
Size: 552 1015 115 1 189 3023
Fragments arranged by size:
3023 1015 552 189 115 1
EcoRI G'AATT_C
Cuts at: 1899 1899
Size: 4895
EcoRV GAT'ATC
Cuts at: 1518 1518
Size: 4895
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2914 2914
Size: 4895
FspI TGC'GCA
Cuts at: 224 2676 224
Size: 2452 2443
HaeII r_GCGC'y
Cuts at: 354 1299 2329 2577 3599 4578 354
Size: 945 1030 248 1022 979 671
Fragments arranged by size:
1030 1022 979 945 671 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 373 405 1296 1328 1615 1647 2737 2767 2769
Size: 32 891 32 287 32 1090 30 2
Cuts at: 2769 2799 373
Size: 30 2469
Fragments arranged by size:
2469 1090 891 287 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1934 1934
Size: 4895
KasI G'GCGC_C
Cuts at: 350 2325 2573 350
Size: 1975 248 2672
Fragments arranged by size:
2672 1975 248
KpnI G_GTAC'C
Cuts at: 1915 1915
Size: 4895
MluI A'CGCG_T
Cuts at: 4328 4328
Size: 4895
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1187 3566 3750 1187
Size: 2379 184 2332
Fragments arranged by size:
2379 2332 184
MscI TGG'CCA
Cuts at: 1771 2656 1771
Size: 885 4010
MslI CAynn'nnrTG
Cuts at: 986 2884 3011 986
Size: 1898 127 2870
Fragments arranged by size:
2870 1898 127
MspA1I CmG'CkG
Cuts at: 484 1047 1098 1513 1721 1767 1803 2006 2320
Size: 563 51 415 208 46 36 203 314
Cuts at: 2320 2680 3693 3938 484
Size: 360 1013 245 1441
Fragments arranged by size:
1441 1013 563 415 360 314 245 208
203 51 46 36
NaeI GCC'GGC
Cuts at: 148 520 1595 3076 148
Size: 372 1075 1481 1967
Fragments arranged by size:
1967 1481 1075 372
NarI GG'CG_CC
Cuts at: 351 2326 2574 351
Size: 1975 248 2672
Fragments arranged by size:
2672 1975 248
NgoMIV G'CCGG_C
Cuts at: 146 518 1593 3074 146
Size: 372 1075 1481 1967
Fragments arranged by size:
1967 1481 1075 372
NspI r_CATG'y
Cuts at: 1948 2448 3355 1948
Size: 500 907 3488
Fragments arranged by size:
3488 907 500
PciI A'CATG_T
Cuts at: 3351 3351
Size: 4895
PflMI CCAn_nnn'nTGG
Cuts at: 747 1194 1767 747
Size: 447 573 3875
Fragments arranged by size:
3875 573 447
PfoI T'CCnGG_A
Cuts at: 1325 1325
Size: 4895
PmeI GTTT'AAAC
Cuts at: 1894 1894
Size: 4895
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1133 1165 4034 4066 1133
Size: 32 2869 32 1962
Fragments arranged by size:
2869 1962 32 32
PpuMI rG'GwC_Cy
Cuts at: 162 714 162
Size: 552 4343
PspOMI G'GGCC_C
Cuts at: 1728 1844 1728
Size: 116 4779
PstI C_TGCA'G
Cuts at: 704 729 974 1271 1292 1876 1942 2627 704
Size: 25 245 297 21 584 66 685 2972
Fragments arranged by size:
2972 685 584 297 245 66 25 21
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4895
PvuII CAG'CTG
Cuts at: 1047 1098 1767 1803 2320 2680 1047
Size: 51 669 36 517 360 3262
Fragments arranged by size:
3262 669 517 360 51 36
RsrII CG'GwC_CG
Cuts at: 3090 3090
Size: 4895
SacI G_AGCT'C
Cuts at: 312 863 1349 1909 312
Size: 551 486 560 3298
Fragments arranged by size:
3298 560 551 486
SalI G'TCGA_C
Cuts at: 1932 1932
Size: 4895
SanDI GG'GwC_CC
Cuts at: 162 162
Size: 4895
SapI GCTCTTCn'nnn_
Cuts at: 2918 3128 2918
Size: 210 4685
SbfI CC_TGCA'GG
Cuts at: 1942 1942
Size: 4895
ScaI AGT'ACT
Cuts at: 4412 4412
Size: 4895
SexAI A'CCwGG_T
Cuts at: 839 839
Size: 4895
SfcI C'TryA_G
Cuts at: 33 700 725 970 1267 1288 1452 1872 1938
Size: 667 25 245 297 21 164 420 66
Cuts at: 1938 2623 3616 3807 33
Size: 685 993 191 1121
Fragments arranged by size:
1121 993 685 667 420 297 245 191
164 66 25 21
SfoI GGC'GCC
Cuts at: 352 2327 2575 352
Size: 1975 248 2672
Fragments arranged by size:
2672 1975 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4895
SmaI CCC'GGG
Cuts at: 159 454 528 1917 159
Size: 295 74 1389 3137
Fragments arranged by size:
3137 1389 295 74
SmlI C'TyrA_G
Cuts at: 1129 2050 2965 3457 3719 1129
Size: 921 915 492 262 2305
Fragments arranged by size:
2305 921 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4895
SphI G_CATG'C
Cuts at: 1948 2448 1948
Size: 500 4395
StuI AGG'CCT
Cuts at: 1111 1111
Size: 4895
StyI C'CwwG_G
Cuts at: 335 375 435 812 2029 2383 335
Size: 40 60 377 1217 354 2847
Fragments arranged by size:
2847 1217 377 354 60 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 631 1657 2478 2611 3070 3279 631
Size: 1026 821 133 459 209 2247
Fragments arranged by size:
2247 1026 821 459 209 133
TatI w'GTAC_w
Cuts at: 4410 4410
Size: 4895
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 400 432 565 597 4412 4444 400
Size: 32 133 32 3815 32 851
Fragments arranged by size:
3815 851 133 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2692 2692
Size: 4895
XbaI T'CTAG_A
Cuts at: 1926 1926
Size: 4895
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 442 1191 2390 442
Size: 749 1199 2947
Fragments arranged by size:
2947 1199 749
XmaI C'CCGG_G
Cuts at: 157 452 526 1915 157
Size: 295 74 1389 3137
Fragments arranged by size:
3137 1389 295 74
ZraI GAC'GTC
Cuts at: 627 627
Size: 4895
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PflMI PfoI PmeI PpiI
PpuMI PspOMI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AflII AhdI AleI AlfI AloI AscI AvrII BaeI
BfrBI BmgBI BmtI BsaXI BsiWI BsrGI BstEII BstZ17I
ClaI CspCI EcoNI FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PmlI PshAI PsiI PspXI PsrI SacII SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI