Restriction map for pF1KB3552
##################################
# NO CUT ENZYMES (  144 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AviII       
BalI        BamHI       BbrPI       BbsI        BciVI       
BclI        BfrI        BglII       BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstYI       BstZ17I     Cfr10I      
ClaI        CpoI        DpnII       DraI        DrdI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        Ksp632I     KspI        
MaeII       MamI        MboI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MunI        MvnI        NaeI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshBI       
Psp1406I    PvuI        RcaI        RsaI        RsrII       
SacII       SalI        SapI        Sau3AI      ScaI        
SexAI       SfuI        SgrAI       SmlI        SnaBI       
SpeI        SspBI       SspI        SwaI        TaqI        
TfiI        Tru9I       Tth111I     TthHB8I     XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AcyI          202  GGCGCC               G[GA]^CG[CT]C
AocI          249  CCTGAGG              CC^T[ACGT]AGG
AvaII         729  GGACC                G^G[AT]CC
AvrII        1021  CCTAGG               C^CTAGG
BbeI          202  GGCGCC               GGCGC^C
BbvCI         496  GCTGAGG              CCTCAGC(-5/-2)
BcgI          150  GCAGCGGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI         1021  CCTAGG               C^CTAGG
BsaHI         202  GGCGCC               G[GA]^CG[CT]C
BsaI           69  GGTCTC               GGTCTC(1/5)
BsmAI          70  GTCTC                GTCTC(1/5)
BsmI          492  GAATGC               GAATGC(1/-1)
Bsu36I        249  CCTGAGG              CC^T[ACGT]AGG
DraIII        584  CACTAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        249  CCTGAGG              CC^T[ACGT]AGG
EcoNI         720  CCTGCACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I         202  GGCGCC               G[GA]^CG[CT]C
KasI          202  GGCGCC               G^GCGCC
MaeIII        433  GTGAC                ^GT[ACGT]AC
NarI          202  GGCGCC               GG^CGCC
NcoI           20  CCATGG               C^CATGG
NspI          606  GCATGC               [GA]CATG^[CT]
PpuMI         728  AGGACCT              [GA]G^G[AT]CC[CT]
PshAI         292  GACAGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI          576  CCTGCAGG             CCTGCA^GG
SfiI          124  GGCCAGACTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          202  GGCGCC               GGC^GCC
SmaI          551  CCCGGG               CCC^GGG
SphI          606  GCATGC               GCATG^C
Sse8387I      576  CCTGCAGG             CCTGCA^GG
StuI          515  AGGCCT               AGG^CCT
Tsp45I        433  GTGAC                ^GT[GC]AC
Tsp509I       321  AATT                 ^AATT
XmaCI         551  CCCGGG               C^CCGGG
XmaI          551  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AspHI         475  GAGCTC               G[AT]GC[AT]^C
              856  GAGCTC              
BanI          202  GGCGCC               G^G[CT][GA]CC
             1012  GGTGCC              
BlpI          601  GCTGAGC              GC^T[ACGT]AGC
              655  GCTGAGC             
Bpu1102I      601  GCTGAGC              GC^T[ACGT]AGC
              655  GCTGAGC             
BsiHKAI       475  GAGCTC               G[AT]GC[AT]^C
              856  GAGCTC              
BsrBI         169  GAGCGG               CCGCTC(-3/-3)
              745  GAGCGG              
CelII         601  GCTGAGC              GC^T[ACGT]AGC
              655  GCTGAGC             
EcoT14I        20  CCATGG               C^C[AT][AT]GG
             1021  CCTAGG              
FauI          371  CCCGC                CCCGC(4/6)
              465  GCGGG               
HgaI          631  GACGC                GACGC(5/10)
             1045  GACGC               
HinfI         976  GAGTC                G^A[ACGT]TC
             1000  GAGTC               
HphI          783  GGTGA                GGTGA(8/7)
              694  TCACC               
MslI          185  CACAAGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1006  CATGCTGGTG          
PleI          976  GAGTC                GAGTC(4/5)
             1000  GAGTC               
PvuII         294  CAGCTG               CAG^CTG
              664  CAGCTG              
SacI          475  GAGCTC               GAGCT^C
              856  GAGCTC              
SfaNI          47  GCATC                GCATC(5/9)
              703  GCATC               
StyI           20  CCATGG               C^C[AT][AT]GG
             1021  CCTAGG              


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AciI        AluI        AlwNI       ApaI        AvaI        
BanII       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BspMI       BsrI        BssKI       BstAPI      BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DraII       DsaI        EaeI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HhaI        HinP1I      HpaII       ItaI        MaeI        
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       PflMI       PstI        
Sau96I      ScrFI       SfcI        TseI        TspRI       
Van91I      
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