Restriction map for pF1KB3552
 (Linear) (Six-base) MAPSORT of: KIBB3552.seq  Check: 5007  from: 1  to: 1119



KIBB3552.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      94     223    1119 
   Size:         94     129     896

  Fragments arranged by size: 

                 896     129      94
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49    1119 
   Size:         49    1070
 
AleI CACnn'nnGTG

Cuts at:      0     189    1119 
   Size:        189     930
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     478     509    1119 
   Size:        478      31     610

  Fragments arranged by size: 

                 610     478      31
 
AlwNI CAG_nnn'CTG

Cuts at:      0     180     627     732     837    1075    1116    1119 
   Size:        180     447     105     105     238      41       3

  Fragments arranged by size: 

                 447     238     180     105     105      41       3
 
ApaI G_GGCC'C

Cuts at:      0     266     689     795     937     955    1119 
   Size:        266     423     106     142      18     164

  Fragments arranged by size: 

                 423     266     164     142     106      18
 
AvaI C'yCGr_G

Cuts at:      0     388     551    1028    1119 
   Size:        388     163     477      91

  Fragments arranged by size: 

                 477     388     163      91
 
AvrII C'CTAG_G

Cuts at:      0    1021    1119 
   Size:       1021      98
 
BanI G'GyrC_C

Cuts at:      0     202    1012    1119 
   Size:        202     810     107

  Fragments arranged by size: 

                 810     202     107
 
BanII G_rGCy'C

Cuts at:      0     266     371     479     689     795     860     937     955 
   Size:        266     105     108     210     106      65      77      18

Cuts at:    955    1119 
   Size:        164

  Fragments arranged by size: 

                 266     210     164     108     106     105      77      65
                  18
 
BbeI G_GCGC'C

Cuts at:      0     206    1119 
   Size:        206     913
 
BbvCI CC'TCA_GC

Cuts at:      0     497    1119 
   Size:        497     622
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     139     173    1119 
   Size:        139      34     946

  Fragments arranged by size: 

                 946     139      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     131     652     799     820     921     941    1119 
   Size:        131     521     147      21     101      20     178

  Fragments arranged by size: 

                 521     178     147     131     101      21      20
 
BlpI GC'TnA_GC

Cuts at:      0     602     656    1119 
   Size:        602      54     463

  Fragments arranged by size: 

                 602     463      54
 
Bme1580I G_kGCm'C

Cuts at:      0     266     648     689     795     937     955    1017    1119 
   Size:        266     382      41     106     142      18      62     102

  Fragments arranged by size: 

                 382     266     142     106     102      62      41      18
            
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     493     670     817     859     947    1078    1119 
   Size:        493     177     147      42      88     131      41

  Fragments arranged by size: 

                 493     177     147     131      88      42      41
 
Bpu10I CC'TnA_GC

Cuts at:      0     478     497     635     824     830    1119 
   Size:        478      19     138     189       6     289

  Fragments arranged by size: 

                 478     289     189     138      19       6
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      75    1119 
   Size:         75    1044
 
BsaHI Gr'CG_yC

Cuts at:      0     203    1119 
   Size:        203     916
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     160     190    1119 
   Size:        160      30     929

  Fragments arranged by size: 

                 929     160      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     276     514     856     988    1119 
   Size:        276     238     342     132     131

  Fragments arranged by size: 

                 342     276     238     132     131
 
BseYI C'CCAG_C

Cuts at:      0     265     274     357     400     504    1016    1103    1119 
   Size:        265       9      83      43     104     512      87      16

  Fragments arranged by size: 

                 512     265     104      87      83      43      16       9
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     438     505     706     784    1119 
   Size:        169     269      67     201      78     335

  Fragments arranged by size: 

                 335     269     201     169      78      67
 
BsiHKAI G_wGCw'C

Cuts at:      0     479     860    1119 
   Size:        479     381     259

  Fragments arranged by size: 

                 479     381     259
 
BsmI GAATG_Cn'

Cuts at:      0     498    1119 
   Size:        498     621
 
Bsp1286I G_dGCh'C

Cuts at:      0     266     371     479     648     689     795     860     937 
   Size:        266     105     108     169      41     106      65      77

Cuts at:    937     955    1017    1119 
   Size:         18      62     102

  Fragments arranged by size: 

                 266     169     108     106     105     102      77      65
                  62      41      18
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      94     223     470     881    1119 
   Size:         94     129     247     411     238

  Fragments arranged by size: 

                 411     247     238     129      94
 
BsrBI CCG'CTC

Cuts at:      0     171     747    1119 
   Size:        171     576     372

  Fragments arranged by size: 

                 576     372     171
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     180     534     589    1075    1119 
   Size:        180     354      55     486      44

  Fragments arranged by size: 

                 486     354     180      55      44
 
Bsu36I CC'TnA_GG

Cuts at:      0     250    1119 
   Size:        250     869
 
BtgI C'CryG_G

Cuts at:      0      20     346     919    1119 
   Size:         20     326     573     200

  Fragments arranged by size: 

                 573     326     200      20
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      87     248    1119 
   Size:         87     161     871

  Fragments arranged by size: 

                 871     161      87
 
BtsI GCAGTG_nn'

Cuts at:      0     596    1119 
   Size:        596     523
 
DraIII CAC_nnn'GTG

Cuts at:      0     589    1119 
   Size:        589     530
 
EaeI y'GGCC_r

Cuts at:      0      17     132     593    1119 
   Size:         17     115     461     526

  Fragments arranged by size: 

                 526     461     115      17
 
EcoICRI GAG'CTC

Cuts at:      0     477     858    1119 
   Size:        477     381     261

  Fragments arranged by size: 

                 477     381     261
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     493     670     817     859     947    1078    1119 
   Size:         49     444     177     147      42      88     131      41

  Fragments arranged by size: 

                 444     177     147     131      88      49      42      41
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     724    1119 
   Size:        724     395
 
EcoO109I rG'GnC_Cy

Cuts at:      0     262     281     468     624     678     686     729     792 
   Size:        262      19     187     156      54       8      43      63

Cuts at:    792     933     934     951     952    1025    1119 
   Size:        141       1      17       1      73      94

  Fragments arranged by size: 

                 262     187     156     141      94      73      63      54
                  43      19      17       8       1       1
 
HaeII r_GCGC'y

Cuts at:      0     206     720     992    1119 
   Size:        206     514     272     127

  Fragments arranged by size: 

                 514     272     206     127
 
KasI G'GCGC_C

Cuts at:      0     202    1119 
   Size:        202     917
 
MslI CAynn'nnrTG

Cuts at:      0     189    1010    1119 
   Size:        189     821     109

  Fragments arranged by size: 

                 821     189     109
 
MspA1I CmG'CkG

Cuts at:      0     153     296     666     673    1119 
   Size:        153     143     370       7     446

  Fragments arranged by size: 

                 446     370     153     143       7
 
NarI GG'CG_CC

Cuts at:      0     203    1119 
   Size:        203     916
 
NcoI C'CATG_G

Cuts at:      0      20    1119 
   Size:         20    1099
 
NspI r_CATG'y

Cuts at:      0     610    1119 
   Size:        610     509
 
PasI CC'CwG_GG

Cuts at:      0     415     682    1119 
   Size:        415     267     437

  Fragments arranged by size: 

                 437     415     267
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      57      96     732    1119 
   Size:         57      39     636     387

  Fragments arranged by size: 

                 636     387      57      39
 
PfoI T'CCnGG_A

Cuts at:      0    1055    1119 
   Size:       1055      64
 
PpuMI rG'GwC_Cy

Cuts at:      0     729    1119 
   Size:        729     390
 
PshAI GACnn'nnGTC

Cuts at:      0     296    1119 
   Size:        296     823
 
PspOMI G'GGCC_C

Cuts at:      0     262     685     791     933     951    1119 
   Size:        262     423     106     142      18     168

  Fragments arranged by size: 

                 423     262     168     142     106      18
 
PstI C_TGCA'G

Cuts at:      0     581     623     665     878    1119 
   Size:        581      42      42     213     241

  Fragments arranged by size: 

                 581     241     213      42      42
 
PvuII CAG'CTG

Cuts at:      0     296     666    1119 
   Size:        296     370     453

  Fragments arranged by size: 

                 453     370     296
 
SacI G_AGCT'C

Cuts at:      0     479     860    1119 
   Size:        479     381     259

  Fragments arranged by size: 

                 479     381     259
 
SbfI CC_TGCA'GG

Cuts at:      0     581    1119 
   Size:        581     538
 
SfcI C'TryA_G

Cuts at:      0     577     619     661     874    1119 
   Size:        577      42      42     213     245

  Fragments arranged by size: 

                 577     245     213      42      42
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     131    1119 
   Size:        131     988
 
SfoI GGC'GCC

Cuts at:      0     204    1119 
   Size:        204     915
 
SmaI CCC'GGG

Cuts at:      0     553    1119 
   Size:        553     566
 
SphI G_CATG'C

Cuts at:      0     610    1119 
   Size:        610     509
 
StuI AGG'CCT

Cuts at:      0     517    1119 
   Size:        517     602
 
StyI C'CwwG_G

Cuts at:      0      20    1021    1119 
   Size:         20    1001      98

  Fragments arranged by size: 

                1001      98      20
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      27     453    1119 
   Size:         27     426     666

  Fragments arranged by size: 

                 666     426      27
 
XmaI C'CCGG_G

Cuts at:      0     551    1119 
   Size:        551     568
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI    AlwNI     ApaI     AvaI    AvrII
     BanI    BanII     BbeI    BbvCI     BcgI     BglI     BlpI Bme1580I
     BpmI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI   BstAPI   Bsu36I     BtgI
    BtgZI     BtsI   DraIII     EaeI  EcoICRI  Eco57MI    EcoNI EcoO109I
    HaeII     KasI     MslI   MspA1I     NarI     NcoI     NspI     PasI
    PflMI     PfoI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI
     SbfI     SfcI     SfiI     SfoI     SmaI     SphI     StuI     StyI
    TaqII     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI
    BamHI     BbsI    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI
     BmtI     BplI    BpuEI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI
    BsmBI    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I     ClaI    CspCI     DraI
     DrdI     EagI     EarI     EciI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     ScaI    SexAI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SrfI
     SspI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmnI     ZraI
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