Restriction map for pF1KB3501
 (Linear) (Six-base) MAPSORT of: KIBB3501.seq  Check: 9649  from: 1  to: 1140



KIBB3501.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     298     740    1140 
   Size:        214      84     442     400

  Fragments arranged by size: 

                 442     400     214      84
 
AflIII A'CryG_T

Cuts at:      0      89     158     849    1140 
   Size:         89      69     691     291

  Fragments arranged by size: 

                 691     291      89      69
 
AlwNI CAG_nnn'CTG

Cuts at:      0     763     863    1086    1140 
   Size:        763     100     223      54

  Fragments arranged by size: 

                 763     223     100      54
 
ApoI r'AATT_y

Cuts at:      0     228     271     308     336    1033    1140 
   Size:        228      43      37      28     697     107

  Fragments arranged by size: 

                 697     228     107      43      37      28
 
AvaI C'yCGr_G

Cuts at:      0      20     138    1140 
   Size:         20     118    1002

  Fragments arranged by size: 

                1002     118      20
 
BamHI G'GATC_C

Cuts at:      0     506     731    1140 
   Size:        506     225     409

  Fragments arranged by size: 

                 506     409     225
 
BanI G'GyrC_C

Cuts at:      0     144    1140 
   Size:        144     996
 
BanII G_rGCy'C

Cuts at:      0    1112    1140 
   Size:       1112      28
 
BbeI G_GCGC'C

Cuts at:      0     148    1140 
   Size:        148     992
 
BfrBI ATG'CAT

Cuts at:      0    1098    1140 
   Size:       1098      42
 
BglII A'GATC_T

Cuts at:      0     996    1140 
   Size:        996     144
 
BlpI GC'TnA_GC

Cuts at:      0      52     895    1140 
   Size:         52     843     245

  Fragments arranged by size: 

                 843     245      52
 
Bme1580I G_kGCm'C

Cuts at:      0     221    1140 
   Size:        221     919
 
BsaAI yAC'GTr

Cuts at:      0      90    1140 
   Size:         90    1050
 
BsaHI Gr'CG_yC

Cuts at:      0     145    1140 
   Size:        145     995
 
BsaWI w'CCGG_w

Cuts at:      0     176     431    1140 
   Size:        176     255     709

  Fragments arranged by size: 

                 709     255     176
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     510     540    1140 
   Size:        510      30     600

  Fragments arranged by size: 

                 600     510      30
 
BseYI C'CCAG_C

Cuts at:      0      36     634    1140 
   Size:         36     598     506

  Fragments arranged by size: 

                 598     506      36
 
BsiHKAI G_wGCw'C

Cuts at:      0     901    1140 
   Size:        901     239
 
BsmI GAATG_Cn'

Cuts at:      0    1100    1140 
   Size:       1100      40
 
Bsp1286I G_dGCh'C

Cuts at:      0     221     901    1112    1140 
   Size:        221     680     211      28

  Fragments arranged by size: 

                 680     221     211      28
 
BsrGI T'GTAC_A

Cuts at:      0     155     914    1140 
   Size:        155     759     226

  Fragments arranged by size: 

                 759     226     155
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      36     642     955    1140 
   Size:         36     606     313     185

  Fragments arranged by size: 

                 606     313     185      36
 
BstYI r'GATC_y

Cuts at:      0     506     731     944     996    1140 
   Size:        506     225     213      52     144

  Fragments arranged by size: 

                 506     225     213     144      52
 
DraI TTT'AAA

Cuts at:      0     704    1140 
   Size:        704     436
 
DraIII CAC_nnn'GTG

Cuts at:      0    1046    1135    1140 
   Size:       1046      89       5

  Fragments arranged by size: 

                1046      89       5
 
EaeI y'GGCC_r

Cuts at:      0     829     964    1140 
   Size:        829     135     176

  Fragments arranged by size: 

                 829     176     135
 
EarI CTCTTCn'nnn_

Cuts at:      0     935    1140 
   Size:        935     205
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     298     740    1140 
   Size:        214      84     442     400

  Fragments arranged by size: 

                 442     400     214      84
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     100    1140 
   Size:        100    1040
 
EcoRI G'AATT_C

Cuts at:      0     228    1140 
   Size:        228     912
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     328    1140 
   Size:        328     812
 
HaeII r_GCGC'y

Cuts at:      0     148    1140 
   Size:        148     992
 
HincII GTy'rAC

Cuts at:      0     783     855    1140 
   Size:        783      72     285

  Fragments arranged by size: 

                 783     285      72
 
HindIII A'AGCT_T

Cuts at:      0     709    1140 
   Size:        709     431
 
KasI G'GCGC_C

Cuts at:      0     144    1140 
   Size:        144     996
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     474     582     843    1140 
   Size:        474     108     261     297

  Fragments arranged by size: 

                 474     297     261     108
 
MslI CAynn'nnrTG

Cuts at:      0     323     404     953    1013    1140 
   Size:        323      81     549      60     127

  Fragments arranged by size: 

                 549     323     127      81      60
 
MspA1I CmG'CkG

Cuts at:      0    1065    1083    1140 
   Size:       1065      18      57

  Fragments arranged by size: 

                1065      57      18
 
NarI GG'CG_CC

Cuts at:      0     145    1140 
   Size:        145     995
 
NsiI A_TGCA'T

Cuts at:      0    1100    1140 
   Size:       1100      40
 
NspI r_CATG'y

Cuts at:      0     162     322     853    1140 
   Size:        162     160     531     287

  Fragments arranged by size: 

                 531     287     162     160
 
PciI A'CATG_T

Cuts at:      0     158     849    1140 
   Size:        158     691     291

  Fragments arranged by size: 

                 691     291     158
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     728    1046    1140 
   Size:        728     318      94

  Fragments arranged by size: 

                 728     318      94
 
PfoI T'CCnGG_A

Cuts at:      0     641    1140 
   Size:        641     499
 
PsiI TTA'TAA

Cuts at:      0     476    1140 
   Size:        476     664
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1020    1052    1140 
   Size:       1020      32      88

  Fragments arranged by size: 

                1020      88      32
 
PvuII CAG'CTG

Cuts at:      0    1065    1083    1140 
   Size:       1065      18      57

  Fragments arranged by size: 

                1065      57      18
 
SfoI GGC'GCC

Cuts at:      0     146    1140 
   Size:        146     994
 
SmaI CCC'GGG

Cuts at:      0      22     140    1140 
   Size:         22     118    1000

  Fragments arranged by size: 

                1000     118      22
 
SspI AAT'ATT

Cuts at:      0     480     652    1140 
   Size:        480     172     488

  Fragments arranged by size: 

                 488     480     172
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     253     471    1140 
   Size:        253     218     669

  Fragments arranged by size: 

                 669     253     218
 
TatI w'GTAC_w

Cuts at:      0     155     914    1140 
   Size:        155     759     226

  Fragments arranged by size: 

                 759     226     155
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     510     542    1140 
   Size:        510      32     598

  Fragments arranged by size: 

                 598     510      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1125    1140 
   Size:       1125      15
 
XbaI T'CTAG_A

Cuts at:      0     201    1140 
   Size:        201     939
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     598    1140 
   Size:        598     542
 
XmaI C'CCGG_G

Cuts at:      0      20     138    1140 
   Size:         20     118    1002

  Fragments arranged by size: 

                1002     118      20
 
XmnI GAAnn'nnTTC

Cuts at:      0     112     311     998    1140 
   Size:        112     199     687     142

  Fragments arranged by size: 

                 687     199     142     112
 

 Enzymes that do cut:

     AcuI   AflIII    AlwNI     ApoI     AvaI    BamHI     BanI    BanII
     BbeI    BfrBI    BglII     BlpI Bme1580I    BsaAI    BsaHI    BsaWI
    BsaXI    BseYI  BsiHKAI     BsmI Bsp1286I    BsrGI    BstXI    BstYI
     DraI   DraIII     EaeI     EarI  Eco57MI    EcoNI    EcoRI     FalI
    HaeII   HincII  HindIII     KasI     MmeI     MslI   MspA1I     NarI
     NsiI     NspI     PciI    PflMI     PfoI     PsiI     PsrI    PvuII
     SfoI     SmaI     SspI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI     BbsI    BbvCI     BcgI    BciVI     BclI
     BglI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BseRI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRV     FseI
     FspI    FspAI    Hin4I     HpaI     KpnI     MfeI     MluI     MscI
     NaeI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI     XhoI
     ZraI
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