Restriction map for pF1KB3451
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# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BbvI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BmyI        BpmI        BsaAI       
BsaHI       BseRI       BsiEI       BsiHKAI     BsiWI       
BsmBI       BsmI        Bsp1286I    Bsp1407I    BspDI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FokI        FseI        
FspI        HaeIII      Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        Ksp632I     
KspI        MaeI        MfeI        MluI        MluNI       
MscI        MseI        MspA1I      MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PstI        PvuI        PvuII       RsaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
TaqI        Tru9I       TseI        Tth111I     TthHB8I     
XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   57 enzymes) #
###################################

AccIII        491  TCCGGA               T^CCGGA
AcsI          247  AAATTC               [GA]^AATT[CT]
ApoI          247  AAATTC               [GA]^AATT[CT]
Asp700I       265  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI          518  CCCGAG               C^[CT]CG[GA]G
BcgI          732  CGAGACAGGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI          466  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      466  GCTCAGC              GC^T[ACGT]AGC
BsaBI         504  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          181  GGTCTC               GGTCTC(1/5)
BsaWI         491  TCCGGA               [AT]^CCGG[AT]
BseAI         491  TCCGGA               T^CCGGA
BsgI          526  GTGCAG               GTGCAG(16/14)
BsiYI         300  CCATGGCATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          300  CCATGGCATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         728  GGGAC                GGGAC(10/14)
BsoBI         518  CCCGAG               C^[CT]CG[GA]G
BspEI         491  TCCGGA               T^CCGGA
BspHI         512  TCATGA               T^CATGA
BspMI         105  ACCTGC               ACCTGC(4/8)
BssKI         149  CCAGG                ^CC[ACGT]GG
BstNI         149  CCAGG                CC^[AT]GG
BstYI         309  GGATCT               [GA]^GATC[CT]
Cac8I         598  GCTTGC               GC[ACGT]^[ACGT]GC
CelII         466  GCTCAGC              GC^T[ACGT]AGC
CfoI           21  GCGC                 GCG^C
DraII         151  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoO109I      151  AGGACCC              [GA]G^G[ACGT]CC[CT]
EcoRII        149  CCAGG                ^CC[AT]GG
Fnu4HI         35  GCGGC                GC^[ACGT]GC
HaeII          20  AGCGCC               [GA]GCGC^[CT]
HapII         492  CCGG                 C^CGG
HgaI          138  GACGC                GACGC(5/10)
HhaI           21  GCGC                 GCG^C
HinP1I         21  GCGC                 G^CGC
HpaII         492  CCGG                 C^CGG
ItaI           35  GCGGC                GC^[ACGT]GC
MaeII         371  ACGT                 A^CGT
MamI          504  GATGGAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          309  GGATCT               [GA]^GATC[CT]
MroI          491  TCCGGA               T^CCGGA
MslI          370  CACGTTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          492  CCGG                 C^CGG
MvaI          149  CCAGG                CC^[AT]GG
PflMI         300  CCATGGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          207  GAGTC                GAGTC(4/5)
PpuMI         151  AGGACCC              [GA]G^G[AT]CC[CT]
PshAI         138  GACGCTCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          512  TCATGA               T^CATGA
ScrFI         149  CCAGG                CC^[ACGT]GG
SfaNI         172  GCATC                GCATC(5/9)
SmlI          184  CTCAAG               C^T[CT][GA]AG
SspI          127  AATATT               AAT^ATT
TspRI         723  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        300  CCATGGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         309  GGATCT               [GA]^GATC[CT]
XmnI          265  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwI           67  GATCC                GGATC(4/5)
              309  GGATC               
AvaII         152  GGACC                G^G[AT]CC
              480  GGTCC               
BbsI          453  GAAGAC               GAAGAC(2/6)
              628  GTCTTC              
BpuAI         453  GAAGAC               GAAGAC(2/6)
              628  GTCTTC              
BsmAI         733  GAGAC                GTCTC(1/5)
              182  GTCTC               
BsrI          724  ACTGG                ACTGG(1/-1)
              775  CCAGT               
Cfr13I        152  GGACC                G^G[ACGT]CC
              480  GGTCC               
DdeI           77  CTAAG                C^T[ACGT]AG
              467  CTCAG               
DpnII          67  GATC                 ^GATC
              310  GATC                
DsaI          300  CCATGG               C^C[GA][CT]GG
              335  CCATGG              
HphI          697  GGTGA                GGTGA(8/7)
              146  TCACC               
MboI           67  GATC                 ^GATC
              310  GATC                
MwoI          651  GCTGAAGAAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              681  GCTCATGGAGC         
NcoI          300  CCATGG               C^CATGG
              335  CCATGG              
NdeII          67  GATC                 ^GATC
              310  GATC                
Sau3AI         67  GATC                 ^GATC
              310  GATC                
Sau96I        152  GGACC                G^G[ACGT]CC
              480  GGTCC               
TfiI          564  GATTC                G^A[AT]TC
              784  GAATC               
Tsp45I        145  GTCAC                ^GT[GC]AC
              320  GTGAC               
XcmI           50  CCAAGAAGAAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              662  CCAAGTTTGAATTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AciI        AluI        BsaJI       EcoT14I     HinfI       
MaeIII      MboII       MnlI        NlaIII      NlaIV       
StyI        Tsp509I     
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