Restriction map for pF1KB3424
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
BbeI        BbrPI       BcgI        BciVI       BcnI        
BglI        BglII       BlpI        Bpu1102I    BsaAI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsmBI       BsoBI       BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     CelII       ClaI        
CpoI        DraI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
EcoT22I     FauI        FseI        FspI        HaeII       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MfeI        MluI        MroI        
MspA1I      MunI        MvnI        MwoI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    StuI        SwaI        TaqI        
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AciI         1035  CCGC                 CCGC(-3/-1)
AcyI          862  GACGCC               G[GA]^CG[CT]C
AflII        1218  CTTAAG               C^TTAAG
ApaI          787  GGGCCC               GGGCC^C
AvrII         777  CCTAGG               C^CTAGG
BalI          986  TGGCCA               TGG^CCA
BanI         1297  GGCACC               G^G[CT][GA]CC
BbsI         1249  GAAGAC               GAAGAC(2/6)
BbvCI         728  CCTCAGC              CCTCAGC(-5/-2)
BclI         1148  TGATCA               T^GATCA
BfrI         1218  CTTAAG               C^TTAAG
BlnI          777  CCTAGG               C^CTAGG
BpmI           32  CTGGAG               CTGGAG(16/14)
BpuAI        1249  GAAGAC               GAAGAC(2/6)
BsaBI         349  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI         862  GACGCC               G[GA]^CG[CT]C
BsaI          544  GAGACC               GGTCTC(1/5)
BseRI         277  GAGGAG               GAGGAG(10/8)
BsiWI         798  CGTACG               C^GTACG
BsmAI         544  GAGAC                GTCTC(1/5)
BsmI          401  GCATTC               GAATGC(1/-1)
Bsp1407I     1195  TGTACA               T^GTACA
BsrFI         173  GCCGGT               [GA]^CCGG[CT]
BsrGI        1195  TGTACA               T^GTACA
BstXI         942  CCAGTTGGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI         1086  GCGC                 GCG^C
Cfr10I        173  GCCGGT               [GA]^CCGG[CT]
DrdI          312  GACCCCGATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          986  TGGCCA               [CT]^GGCC[GA]
EcoNI         682  CCTGGCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         229  GAATTC               G^AATTC
FbaI         1148  TGATCA               T^GATCA
HapII         174  CCGG                 C^CGG
HhaI         1086  GCGC                 GCG^C
Hin1I         862  GACGCC               G[GA]^CG[CT]C
HinP1I       1086  GCGC                 G^CGC
HpaII         174  CCGG                 C^CGG
MamI          349  GATGACAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         986  TGGCCA               TGG^CCA
MscI          986  TGGCCA               TGG^CCA
MslI          513  CACAGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          174  CCGG                 C^CGG
PleI          886  GACTC                GAGTC(4/5)
PpuMI         310  GGGACCC              [GA]G^G[AT]CC[CT]
SphI          468  GCATGC               GCATG^C
SspBI        1195  TGTACA               T^GTACA
SspI          626  AATATT               AAT^ATT


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI          369  GTCTAC               GT^[AC][GT]AC
              415  GTAGAC              
AluI          605  AGCT                 AG^CT
             1203  AGCT                
AlwNI         128  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1199  CAGAAGCTG           
AocI          655  CCTAAGG              CC^T[ACGT]AGG
             1303  CCTGAGG             
Asp700I       760  GAACTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1270  GAAAATGTTC          
BamHI         501  GGATCC               G^GATCC
             1011  GGATCC              
BanII         381  GGGCTC               G[GA]GC[CT]^C
              787  GGGCCC              
BbvI          133  GCTGC                GCAGC(8/12)
              440  GCTGC               
BmyI          381  GGGCTC               G[AGT]GC[ACT]^C
              787  GGGCCC              
Bsp1286I      381  GGGCTC               G[AGT]GC[ACT]^C
              787  GGGCCC              
BsrI          150  ACTGG                ACTGG(1/-1)
              942  CCAGT               
Bsu36I        655  CCTAAGG              CC^T[ACGT]AGG
             1303  CCTGAGG             
DraII         310  GGGACCC              [GA]G^G[ACGT]CC[CT]
              533  AGGCCCC             
DraIII        165  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              460  CACAAGGTG           
EarI          337  GAAGAG               CTCTTC(1/4)
              634  GAAGAG              
Eco81I        655  CCTAAGG              CC^T[ACGT]AGG
             1303  CCTGAGG             
EcoO109I      310  GGGACCC              [GA]G^G[ACGT]CC[CT]
              533  AGGCCCC             
EcoT14I       332  CCTTGG               C^C[AT][AT]GG
              777  CCTAGG              
HgaI          862  GACGC                GACGC(5/10)
             1116  GACGC               
Ksp632I       337  GAAGAG               CTCTTC(1/4)
              634  GAAGAG              
MaeII         395  ACGT                 A^CGT
             1227  ACGT                
NspI          468  GCATGC               [GA]CATG^[CT]
              828  ACATGC              
SfaNI         130  GATGC                GCATC(5/9)
             1088  GCATC               
SmlI          741  CTTGAG               C^T[CT][GA]AG
             1218  CTTAAG              
StyI          332  CCTTGG               C^C[AT][AT]GG
              777  CCTAGG              
TseI          133  GCTGC                G^C[AT]GC
              440  GCTGC               
Tsp45I        154  GTGAC                ^GT[GC]AC
              511  GTCAC               
XmnI          760  GAACTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1270  GAAAATGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   45 enzymes) #
#########################################

AcsI        AfaI        AlwI        ApoI        AvaII       
BfaI        BsaJI       BsiYI       BslI        BsmFI       
BssKI       BstF5I      BstNI       BstYI       Cac8I       
Cfr13I      DdeI        DpnII       EcoRII      Fnu4HI      
FokI        HaeIII      HinfI       HphI        ItaI        
MaeI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MvaI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
TfiI        Tru9I       Tsp509I     TspRI       XhoII       
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