Restriction map for pF1KB3413
##################################
# NO CUT ENZYMES (  145 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaI        BseAI       BseRI       
BsgI        BsiWI       BsmAI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
KasI        KpnI        MaeI        MaeII       MamI        
MfeI        MluI        MroI        MseI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       Tth111I     
XbaI        XcmI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AlwNI         619  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          338  GGGCCC               GGGCC^C
AspHI         487  GAGCTC               G[AT]GC[AT]^C
BanI          473  GGCACC               G^G[CT][GA]CC
BsaWI         110  ACCGGA               [AT]^CCGG[AT]
BsiEI         108  CGACCG               CG[GA][CT]^CG
BsiHKAI       487  GAGCTC               G[AT]GC[AT]^C
BssSI          96  CTCGTG               CACGAG(-5/-1)
DdeI          492  CTTAG                C^T[ACGT]AG
DraIII        585  CACTGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI           77  CCGCGG               C^C[GA][CT]GG
EcoT14I       938  CCAAGG               C^C[AT][AT]GG
HaeII         620  AGCGCC               [GA]GCGC^[CT]
HincII        916  GTCAAC               GT[CT]^[GA]AC
HindII        916  GTCAAC               GT[CT]^[GA]AC
HphI          139  GGTGA                GGTGA(8/7)
KspI           77  CCGCGG               CCGC^GG
MslI          310  CATGAAAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I        301  CTCGAG               C^TCGAG
PpuMI          82  GGGACCC              [GA]G^G[AT]CC[CT]
PstI          963  CTGCAG               CTGCA^G
SacI          487  GAGCTC               GAGCT^C
SacII          77  CCGCGG               CCGC^GG
SfcI          963  CTGCAG               C^T[GA][CT]AG
SphI          697  GCATGC               GCATG^C
StuI         1011  AGGCCT               AGG^CCT
StyI          938  CCAAGG               C^C[AT][AT]GG
TaqI          302  TCGA                 T^CGA
Tsp45I        140  GTGAC                ^GT[GC]AC
Tsp509I       220  AATT                 ^AATT
TthHB8I       302  TCGA                 T^CGA
XhoI          301  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcyI          627  GGCGTC               G[GA]^CG[CT]C
              662  GGCGTC              
AvaI          301  CTCGAG               C^[CT]CG[GA]G
              609  CCCGAG              
BamHI         843  GGATCC               G^GATCC
              855  GGATCC              
BsaHI         627  GGCGTC               G[GA]^CG[CT]C
              662  GGCGTC              
BsoBI         301  CTCGAG               C^[CT]CG[GA]G
              609  CCCGAG              
BsrDI         771  CATTGC               GCAATG(2/0)
              680  GCAATG              
DraII          82  GGGACCC              [GA]G^G[ACGT]CC[CT]
              338  GGGCCCC             
EarI          406  GAAGAG               CTCTTC(1/4)
              538  GAAGAG              
EcoO109I       82  GGGACCC              [GA]G^G[ACGT]CC[CT]
              338  GGGCCCC             
Hin1I         627  GGCGTC               G[GA]^CG[CT]C
              662  GGCGTC              
Ksp632I       406  GAAGAG               CTCTTC(1/4)
              538  GAAGAG              
MaeIII        140  GTGAC                ^GT[ACGT]AC
              292  GTAAC               
NspI          462  GCATGT               [GA]CATG^[CT]
              697  GCATGC              
PflMI         584  CCACTGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              618  CCAGCGCCTGG         
PleI            7  GACTC                GAGTC(4/5)
               46  GACTC               
PvuII         144  CAGCTG               CAG^CTG
              784  CAGCTG              
TfiI          116  GAATC                G^A[AT]TC
              580  GAATC               
Van91I        584  CCACTGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              618  CCAGCGCCTGG         


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaII       BalI        BanII       BbvI        BcnI        
BmyI        BsaJI       BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CfoI        Cfr13I      
DpnII       EaeI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       ItaI        MboI        
MboII       MflI        MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
TseI        TspRI       XhoII       
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