Restriction map for pF1KB3413
 (Linear) (Six-base) MAPSORT of: KIBB3413.seq  Check: 338  from: 1  to: 1017



KIBB3413.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191    1017 
   Size:        191     826
 
AlwNI CAG_nnn'CTG

Cuts at:      0     624    1017 
   Size:        624     393
 
ApaI G_GGCC'C

Cuts at:      0     342    1017 
   Size:        342     675
 
AvaI C'yCGr_G

Cuts at:      0     301     609    1017 
   Size:        301     308     408

  Fragments arranged by size: 

                 408     308     301
 
BamHI G'GATC_C

Cuts at:      0     843     855    1017 
   Size:        843      12     162

  Fragments arranged by size: 

                 843     162      12
 
BanI G'GyrC_C

Cuts at:      0     473    1017 
   Size:        473     544
 
BanII G_rGCy'C

Cuts at:      0     342     491     798    1017 
   Size:        342     149     307     219

  Fragments arranged by size: 

                 342     307     219     149
 
Bme1580I G_kGCm'C

Cuts at:      0     133     342    1017 
   Size:        133     209     675

  Fragments arranged by size: 

                 675     209     133
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     558     710    1017 
   Size:        558     152     307

  Fragments arranged by size: 

                 558     307     152
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     589     738    1017 
   Size:        589     149     279

  Fragments arranged by size: 

                 589     279     149
 
BsaHI Gr'CG_yC

Cuts at:      0     628     663    1017 
   Size:        628      35     354

  Fragments arranged by size: 

                 628     354      35
 
BsaWI w'CCGG_w

Cuts at:      0     110    1017 
   Size:        110     907
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      31      61    1017 
   Size:         31      30     956

  Fragments arranged by size: 

                 956      31      30
 
BseYI C'CCAG_C

Cuts at:      0      21    1017 
   Size:         21     996
 
BsiEI CG_ry'CG

Cuts at:      0     111    1017 
   Size:        111     906
 
BsiHKAI G_wGCw'C

Cuts at:      0     491    1017 
   Size:        491     526
 
Bsp1286I G_dGCh'C

Cuts at:      0     133     342     491     798    1017 
   Size:        133     209     149     307     219

  Fragments arranged by size: 

                 307     219     209     149     133
 
BsrDI GCAATG_nn'

Cuts at:      0     687     770    1017 
   Size:        687      83     247

  Fragments arranged by size: 

                 687     247      83
 
BssSI C'ACGA_G

Cuts at:      0      96    1017 
   Size:         96     921
 
BstYI r'GATC_y

Cuts at:      0     149     843     855    1017 
   Size:        149     694      12     162

  Fragments arranged by size: 

                 694     162     149      12
 
BtgI C'CryG_G

Cuts at:      0      77    1017 
   Size:         77     940
 
BtsI GCAGTG_nn'

Cuts at:      0     972    1017 
   Size:        972      45
 
DraIII CAC_nnn'GTG

Cuts at:      0     590    1017 
   Size:        590     427
 
EaeI y'GGCC_r

Cuts at:      0     359     615     800    1017 
   Size:        359     256     185     217

  Fragments arranged by size: 

                 359     256     217     185
 
EarI CTCTTCn'nnn_

Cuts at:      0     401     533    1017 
   Size:        401     132     484

  Fragments arranged by size: 

                 484     401     132
 
EcoICRI GAG'CTC

Cuts at:      0     489    1017 
   Size:        489     528
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     191    1017 
   Size:        191     826
 
EcoO109I rG'GnC_Cy

Cuts at:      0      83     339    1017 
   Size:         83     256     678

  Fragments arranged by size: 

                 678     256      83
 
HaeII r_GCGC'y

Cuts at:      0     624    1017 
   Size:        624     393
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     310     342     780     812     835     867    1017 
   Size:        310      32     438      32      23      32     150

  Fragments arranged by size: 

                 438     310     150      32      32      32      23
 
HincII GTy'rAC

Cuts at:      0     918    1017 
   Size:        918      99
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     700    1017 
   Size:        700     317
 
MscI TGG'CCA

Cuts at:      0     361     617     802    1017 
   Size:        361     256     185     215

  Fragments arranged by size: 

                 361     256     215     185
 
MslI CAynn'nnrTG

Cuts at:      0     314    1017 
   Size:        314     703
 
MspA1I CmG'CkG

Cuts at:      0      53      79     146     704     786    1002    1017 
   Size:         53      26      67     558      82     216      15

  Fragments arranged by size: 

                 558     216      82      67      53      26      15
 
NspI r_CATG'y

Cuts at:      0     466     701    1017 
   Size:        466     235     316

  Fragments arranged by size: 

                 466     316     235
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     590     624    1017 
   Size:        590      34     393

  Fragments arranged by size: 

                 590     393      34
 
PpuMI rG'GwC_Cy

Cuts at:      0      83    1017 
   Size:         83     934
 
PspOMI G'GGCC_C

Cuts at:      0     338    1017 
   Size:        338     679
 
PstI C_TGCA'G

Cuts at:      0     967    1017 
   Size:        967      50
 
PvuII CAG'CTG

Cuts at:      0     146     786    1017 
   Size:        146     640     231

  Fragments arranged by size: 

                 640     231     146
 
SacI G_AGCT'C

Cuts at:      0     491    1017 
   Size:        491     526
 
SacII CC_GC'GG

Cuts at:      0      80    1017 
   Size:         80     937
 
SanDI GG'GwC_CC

Cuts at:      0      83    1017 
   Size:         83     934
 
SfcI C'TryA_G

Cuts at:      0     963    1017 
   Size:        963      54
 
SmlI C'TyrA_G

Cuts at:      0     301     568     753    1017 
   Size:        301     267     185     264

  Fragments arranged by size: 

                 301     267     264     185
 
SphI G_CATG'C

Cuts at:      0     701    1017 
   Size:        701     316
 
StuI AGG'CCT

Cuts at:      0    1013    1017 
   Size:       1013       4
 
StyI C'CwwG_G

Cuts at:      0     938    1017 
   Size:        938      79
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     491    1017 
   Size:        491     526
 
TatI w'GTAC_w

Cuts at:      0     733    1017 
   Size:        733     284
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      31      63    1017 
   Size:         31      32     954

  Fragments arranged by size: 

                 954      32      31
 
XhoI C'TCGA_G

Cuts at:      0     301    1017 
   Size:        301     716
 

 Enzymes that do cut:

     AcuI    AlwNI     ApaI     AvaI    BamHI     BanI    BanII Bme1580I
     BmrI    BpuEI    BsaHI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BsrDI    BssSI    BstYI     BtgI     BtsI   DraIII     EaeI
     EarI  EcoICRI  Eco57MI EcoO109I    HaeII    Hin4I   HincII     MmeI
     MscI     MslI   MspA1I     NspI    PflMI    PpuMI   PspOMI     PstI
    PvuII     SacI    SacII    SanDI     SfcI     SmlI     SphI     StuI
     StyI    TaqII     TatI     TstI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI
     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI    BseRI     BsgI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI     DrdI     EagI     EciI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI
     PvuI    RsrII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     SwaI  Tth111I     XbaI     XcmI     XmaI     XmnI     ZraI
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