Restriction map for pF1KB3406
 (Linear) (Six-base) MAPSORT of: KIBB3406.seq  Check: 8259  from: 1  to: 1101



KIBB3406.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AflII C'TTAA_G

Cuts at:      0      91    1101 
   Size:         91    1010
 
AlwNI CAG_nnn'CTG

Cuts at:      0     345    1101 
   Size:        345     756
 
ApoI r'AATT_y

Cuts at:      0     283     828     878     994    1101 
   Size:        283     545      50     116     107

  Fragments arranged by size: 

                 545     283     116     107      50
 
AseI AT'TA_AT

Cuts at:      0     566    1101 
   Size:        566     535
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     312     345    1101 
   Size:        312      33     756

  Fragments arranged by size: 

                 756     312      33
 
BanI G'GyrC_C

Cuts at:      0     328     370     789    1101 
   Size:        328      42     419     312

  Fragments arranged by size: 

                 419     328     312      42
 
BglII A'GATC_T

Cuts at:      0     851    1101 
   Size:        851     250
 
BlpI GC'TnA_GC

Cuts at:      0     107    1101 
   Size:        107     994
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     916    1101 
   Size:        916     185
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     924    1101 
   Size:        924     177
 
Bpu10I CC'TnA_GC

Cuts at:      0      12    1101 
   Size:         12    1089
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1024    1101 
   Size:       1024      77
 
BsaAI yAC'GTr

Cuts at:      0     664    1101 
   Size:        664     437
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     742     772    1101 
   Size:        742      30     329

  Fragments arranged by size: 

                 742     329      30
 
BsmI GAATG_Cn'

Cuts at:      0     506     620    1101 
   Size:        506     114     481

  Fragments arranged by size: 

                 506     481     114
 
BsrDI GCAATG_nn'

Cuts at:      0     404    1101 
   Size:        404     697
 
BstYI r'GATC_y

Cuts at:      0     335     348     851    1101 
   Size:        335      13     503     250

  Fragments arranged by size: 

                 503     335     250      13
 
BtsI GCAGTG_nn'

Cuts at:      0     235     416    1101 
   Size:        235     181     685

  Fragments arranged by size: 

                 685     235     181
 
DraI TTT'AAA

Cuts at:      0     999    1101 
   Size:        999     102
 
EaeI y'GGCC_r

Cuts at:      0     176    1101 
   Size:        176     925
 
EarI CTCTTCn'nnn_

Cuts at:      0     610    1101 
   Size:        610     491
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     924    1101 
   Size:        924     177
 
EcoRI G'AATT_C

Cuts at:      0     283    1101 
   Size:        283     818
 
EcoRV GAT'ATC

Cuts at:      0     777    1101 
   Size:        777     324
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     704     736     742     774     901     933    1101 
   Size:        704      32       6      32     127      32     168

  Fragments arranged by size: 

                 704     168     127      32      32      32       6
 
HincII GTy'rAC

Cuts at:      0     233    1101 
   Size:        233     868
 
HindIII A'AGCT_T

Cuts at:      0     109    1101 
   Size:        109     992
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1046    1101 
   Size:       1046      55
 
MscI TGG'CCA

Cuts at:      0     178    1101 
   Size:        178     923
 
MslI CAynn'nnrTG

Cuts at:      0     409     694    1101 
   Size:        409     285     407

  Fragments arranged by size: 

                 409     407     285
 
MspA1I CmG'CkG

Cuts at:      0     679     824    1101 
   Size:        679     145     277

  Fragments arranged by size: 

                 679     277     145
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     139     345    1065    1101 
   Size:        139     206     720      36

  Fragments arranged by size: 

                 720     206     139      36
 
PfoI T'CCnGG_A

Cuts at:      0     156     344     940    1101 
   Size:        156     188     596     161

  Fragments arranged by size: 

                 596     188     161     156
 
PvuII CAG'CTG

Cuts at:      0     679    1101 
   Size:        679     422
 
SapI GCTCTTCn'nnn_

Cuts at:      0     610    1101 
   Size:        610     491
 
SfcI C'TryA_G

Cuts at:      0     674    1101 
   Size:        674     427
 
SmlI C'TyrA_G

Cuts at:      0      91    1101 
   Size:         91    1010
 
SnaBI TAC'GTA

Cuts at:      0     664    1101 
   Size:        664     437
 
SpeI A'CTAG_T

Cuts at:      0     870    1101 
   Size:        870     231
 
StyI C'CwwG_G

Cuts at:      0     374    1101 
   Size:        374     727
 
SwaI ATTT'AAAT

Cuts at:      0     999    1101 
   Size:        999     102
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     768     948    1101 
   Size:        768     180     153

  Fragments arranged by size: 

                 768     180     153
 

 Enzymes that do cut:

    AflII    AlwNI     ApoI     AseI     BaeI     BanI    BglII     BlpI
     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaXI     BsmI    BsrDI
    BstYI     BtsI     DraI     EaeI     EarI  Eco57MI    EcoRI    EcoRV
    Hin4I   HincII  HindIII     MmeI     MscI     MslI   MspA1I    PflMI
     PfoI    PvuII     SapI     SfcI     SmlI    SnaBI     SpeI     StyI
     SwaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI Bme1580I    BmgBI     BmtI
     BplI    BpuEI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I     FalI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SphI     SrfI     SspI
     StuI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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