Restriction map for pF1KB3399
 (Circular) (Six-base) MAPSORT of: pF1KB3399.seq  Check: 4876  from: 1  to: 3671



pF1KB3399  3671 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:23  ..

AccI GT'mk_AC

Cuts at:    709     709 
   Size:       3671
 
Acc65I G'GTAC_C

Cuts at:    687     687 
   Size:       3671
 
AclI AA'CG_TT

Cuts at:    419    3511     419 
   Size:       3092     579
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1496    1928    2675    2943    1496 
   Size:        432     747     268    2224

  Fragments arranged by size: 

                2224     747     432     268
 
AflII C'TTAA_G

Cuts at:    189     189 
   Size:       3671
 
AflIII A'CryG_T

Cuts at:   1023    2127    3104    1023 
   Size:       1104     977    1590

  Fragments arranged by size: 

                1590    1104     977
 
AgeI A'CCGG_T

Cuts at:   2059    2059 
   Size:       3671
 
AlwNI CAG_nnn'CTG

Cuts at:    976    2543     976 
   Size:       1567    2104
 
ApaI G_GGCC'C

Cuts at:    593     593 
   Size:       3671
 
ApaLI G'TGCA_C

Cuts at:   2441    2441 
   Size:       3671
 
ApoI r'AATT_y

Cuts at:    340     463     659     675    3024    3280     340 
   Size:        123     196      16    2349     256     731

  Fragments arranged by size: 

                2349     731     256     196     123      16
 
AseI AT'TA_AT

Cuts at:     20     250      20 
   Size:        230    3441
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3671
 
AvaI C'yCGr_G

Cuts at:    691     691 
   Size:       3671
 
BamHI G'GATC_C

Cuts at:    696     696 
   Size:       3671
 
BanI G'GyrC_C

Cuts at:    346     687    1101    1349    1384     346 
   Size:        341     414     248      35    2633

  Fragments arranged by size: 

                2633     414     341     248      35
 
BanII G_rGCy'C

Cuts at:    593     685    1715     593 
   Size:         92    1030    2549

  Fragments arranged by size: 

                2549    1030      92
 
BbeI G_GCGC'C

Cuts at:   1105    1353    1105 
   Size:        248    3423
 
BciVI GTATCCnnnnn_n'

Cuts at:   1569    2330    3671    1569 
   Size:        761    1341    1569

  Fragments arranged by size: 

                1569    1341     761
 
BclI T'GATC_A

Cuts at:   1191    1191 
   Size:       3671
 
BglII A'GATC_T

Cuts at:   1186    3223    1186 
   Size:       2037    1634
 
BlpI GC'TnA_GC

Cuts at:    357     783     357 
   Size:        426    3245
 
Bme1580I G_kGCm'C

Cuts at:    593    1296    1389    2445     593 
   Size:        703      93    1056    1819

  Fragments arranged by size: 

                1819    1056     703      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    883     964    1299    3443     883 
   Size:         81     335    2144    1111

  Fragments arranged by size: 

                2144    1111     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2038    2038 
   Size:       3671
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    335     335 
   Size:       3671
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    847    1726    2218    2516     847 
   Size:        879     492     298    2002

  Fragments arranged by size: 

                2002     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3375    3375 
   Size:       3671
 
BsaAI yAC'GTr

Cuts at:   1024    1024 
   Size:       3671
 
BsaBI GATnn'nnATC

Cuts at:      6    1185    3222       6 
   Size:       1179    2037     455

  Fragments arranged by size: 

                2037    1179     455
 
BsaHI Gr'CG_yC

Cuts at:   1102    1350    2052    3548    1102 
   Size:        248     702    1496    1225

  Fragments arranged by size: 

                1496    1225     702     248
 
BsaWI w'CCGG_w

Cuts at:    860    1381    2059    2333    2480    2891     860 
   Size:        521     678     274     147     411    1640

  Fragments arranged by size: 

                1640     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    203     233     203 
   Size:         30    3641
 
BseYI C'CCAG_C

Cuts at:   1096    1854    2431    2927    1096 
   Size:        758     577     496    1840

  Fragments arranged by size: 

                1840     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1213    1259    2467     104 
   Size:       1109      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1109      46
 
BsiHKAI G_wGCw'C

Cuts at:    685    1463    1653    2445     685 
   Size:        778     190     792    1911

  Fragments arranged by size: 

                1911     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    593     685    1296    1389    1463    1653    1715    2445     593 
   Size:         92     611      93      74     190      62     730    1819

  Fragments arranged by size: 

                1819     730     611     190      93      92      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2891    2891 
   Size:       3671
 
BspHI T'CATG_A

Cuts at:   2847    2847 
   Size:       3671
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    721     935    1237    1618     721 
   Size:        214     302     381    2774

  Fragments arranged by size: 

                2774     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1963    2017    3501    3666      50 
   Size:       1913      54    1484     165      55

  Fragments arranged by size: 

                1913    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1583    1583 
   Size:       3671
 
BsrFI r'CCGG_y

Cuts at:   1669    1850    2059    1669 
   Size:        181     209    3281

  Fragments arranged by size: 

                3281     209     181
 
BssSI C'ACGA_G

Cuts at:   1942    2300    1942 
   Size:        358    3313
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1048    1048 
   Size:       3671
 
BstBI TT'CG_AA

Cuts at:   2032    2032 
   Size:       3671
 
BstYI r'GATC_y

Cuts at:      1     696    1163    1186    1521    1767    2779    2865    2877 
   Size:        695     467      23     335     246    1012      86      12

Cuts at:   2877    3223       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     695     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    294     294 
   Size:       3671
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3671
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1621    1802    1965    1621 
   Size:        181     163    3327

  Fragments arranged by size: 

                3327     181     163
 
BtsI GCAGTG_nn'

Cuts at:    434     914     434 
   Size:        480    3191
 
DraI TTT'AAA

Cuts at:    185     670     185 
   Size:        485    3186
 
DrdI GACnn_nn'nnGTC

Cuts at:   1377    2235    1377 
   Size:        858    2813
 
EaeI y'GGCC_r

Cuts at:   1256    1430    1821    1848    3193    1256 
   Size:        174     391      27    1345    1734

  Fragments arranged by size: 

                1734    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1256    1256 
   Size:       3671
 
EarI CTCTTCn'nnn_

Cuts at:   1694    1904    1694 
   Size:        210    3461
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1879    2189    2335    3481    1879 
   Size:        310     146    1146    2069

  Fragments arranged by size: 

                2069    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    683     683 
   Size:       3671
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    335    1496    1928    2675    2943     335 
   Size:       1161     432     747     268    1063

  Fragments arranged by size: 

                1161    1063     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    589     810     589 
   Size:        221    3450
 
EcoRI G'AATT_C

Cuts at:    463     675     463 
   Size:        212    3459
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1690    1690 
   Size:       3671
 
FspI TGC'GCA

Cuts at:   1452    1452 
   Size:       3671
 
HaeII r_GCGC'y

Cuts at:   1105    1353    2375    3354    1105 
   Size:        248    1022     979    1422

  Fragments arranged by size: 

                1422    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    522     554    1513    1543    1545    1575     522 
   Size:         32     959      30       2      30    2618

  Fragments arranged by size: 

                2618     959      32      30      30       2
 
HincII GTy'rAC

Cuts at:    417     710     417 
   Size:        293    3378
 
HindIII A'AGCT_T

Cuts at:    256     256 
   Size:       3671
 
HpaI GTT'AAC

Cuts at:    417     417 
   Size:       3671
 
KasI G'GCGC_C

Cuts at:   1101    1349    1101 
   Size:        248    3423
 
KpnI G_GTAC'C

Cuts at:    691     691 
   Size:       3671
 
MluI A'CGCG_T

Cuts at:   3104    3104 
   Size:       3671
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    219    2342    2526     219 
   Size:       2123     184    1364

  Fragments arranged by size: 

                2123    1364     184
 
MscI TGG'CCA

Cuts at:   1432    1432 
   Size:       3671
 
MslI CAynn'nnrTG

Cuts at:   1660    1787    1660 
   Size:        127    3544
 
MspA1I CmG'CkG

Cuts at:    782    1096    1456    2469    2714     782 
   Size:        314     360    1013     245    1739

  Fragments arranged by size: 

                1739    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1852    1852 
   Size:       3671
 
NarI GG'CG_CC

Cuts at:   1102    1350    1102 
   Size:        248    3423
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3671
 
NgoMIV G'CCGG_C

Cuts at:   1850    1850 
   Size:       3671
 
NspI r_CATG'y

Cuts at:    441     724    1224    2131     441 
   Size:        283     500     907    1981

  Fragments arranged by size: 

                1981     907     500     283
 
PciI A'CATG_T

Cuts at:   2127    2127 
   Size:       3671
 
PmeI GTTT'AAAC

Cuts at:    670     670 
   Size:       3671
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2810    2842    2810 
   Size:         32    3639
 
PsiI TTA'TAA

Cuts at:    227     227 
   Size:       3671
 
PspOMI G'GGCC_C

Cuts at:    589     589 
   Size:       3671
 
PstI C_TGCA'G

Cuts at:    374     718    1403     374 
   Size:        344     685    2642

  Fragments arranged by size: 

                2642     685     344
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3671
 
PvuII CAG'CTG

Cuts at:   1096    1456    1096 
   Size:        360    3311
 
RsrII CG'GwC_CG

Cuts at:   1866    1866 
   Size:       3671
 
SacI G_AGCT'C

Cuts at:    685     685 
   Size:       3671
 
SalI G'TCGA_C

Cuts at:    708     708 
   Size:       3671
 
SapI GCTCTTCn'nnn_

Cuts at:   1694    1904    1694 
   Size:        210    3461
 
SbfI CC_TGCA'GG

Cuts at:    718     718 
   Size:       3671
 
ScaI AGT'ACT

Cuts at:   3188    3188 
   Size:       3671
 
SfcI C'TryA_G

Cuts at:     33     370     714    1399    2392    2583      33 
   Size:        337     344     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     344     337     191
 
SfoI GGC'GCC

Cuts at:   1103    1351    1103 
   Size:        248    3423
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3671
 
SmaI CCC'GGG

Cuts at:    693     693 
   Size:       3671
 
SmlI C'TyrA_G

Cuts at:    189     826    1741    2233    2495     189 
   Size:        637     915     492     262    1365

  Fragments arranged by size: 

                1365     915     637     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3671
 
SphI G_CATG'C

Cuts at:    724    1224     724 
   Size:        500    3171
 
SspI AAT'ATT

Cuts at:    395     395 
   Size:       3671
 
StyI C'CwwG_G

Cuts at:    107     805    1159     107 
   Size:        698     354    2619

  Fragments arranged by size: 

                2619     698     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1254    1387    1846    2055    1254 
   Size:        133     459     209    2870

  Fragments arranged by size: 

                2870     459     209     133
 
TatI w'GTAC_w

Cuts at:   3186    3186 
   Size:       3671
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3188    3220    3188 
   Size:         32    3639
 
Tth111I GACn'n_nGTC

Cuts at:   1468    1468 
   Size:       3671
 
XbaI T'CTAG_A

Cuts at:    702     702 
   Size:       3671
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1166    1166 
   Size:       3671
 
XmaI C'CCGG_G

Cuts at:    691     691 
   Size:       3671
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI    AlwNI
     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI     PmeI     PpiI     PsiI   PspOMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI     AhdI     AleI     AlfI     AloI     AscI
    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI   Bpu10I    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     StuI     SwaI     XhoI     XmnI     ZraI
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