Restriction map for
pF1KB3385
(Circular) (Six-base) MAPSORT of: pF1KB3385.seq Check: 5128 from: 1 to: 3677
pF1KB3385 3677 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AccI GT'mk_AC
Cuts at: 715 715
Size: 3677
Acc65I G'GTAC_C
Cuts at: 390 693 390
Size: 303 3374
AclI AA'CG_TT
Cuts at: 671 3517 671
Size: 2846 831
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 1502 1934 2681 2949 421
Size: 1081 432 747 268 1149
Fragments arranged by size:
1149 1081 747 432 268
AflIII A'CryG_T
Cuts at: 1029 2133 3110 1029
Size: 1104 977 1596
Fragments arranged by size:
1596 1104 977
AgeI A'CCGG_T
Cuts at: 2065 2065
Size: 3677
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 535 535
Size: 3677
AlwNI CAG_nnn'CTG
Cuts at: 982 2549 982
Size: 1567 2110
ApaI G_GGCC'C
Cuts at: 435 435
Size: 3677
ApaLI G'TGCA_C
Cuts at: 410 2447 410
Size: 2037 1640
ApoI r'AATT_y
Cuts at: 681 3030 3286 681
Size: 2349 256 1072
Fragments arranged by size:
2349 1072 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3677
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3677
AvaI C'yCGr_G
Cuts at: 697 697
Size: 3677
BamHI G'GATC_C
Cuts at: 702 702
Size: 3677
BanI G'GyrC_C
Cuts at: 390 693 1107 1355 1390 390
Size: 303 414 248 35 2677
Fragments arranged by size:
2677 414 303 248 35
BanII G_rGCy'C
Cuts at: 161 435 691 1721 161
Size: 274 256 1030 2117
Fragments arranged by size:
2117 1030 274 256
BbeI G_GCGC'C
Cuts at: 1111 1359 1111
Size: 248 3429
BciVI GTATCCnnnnn_n'
Cuts at: 1575 2336 3677 1575
Size: 761 1341 1575
Fragments arranged by size:
1575 1341 761
BclI T'GATC_A
Cuts at: 1197 1197
Size: 3677
BglII A'GATC_T
Cuts at: 1192 3229 1192
Size: 2037 1640
BlpI GC'TnA_GC
Cuts at: 789 789
Size: 3677
Bme1580I G_kGCm'C
Cuts at: 414 435 1302 1395 2451 414
Size: 21 867 93 1056 1640
Fragments arranged by size:
1640 1056 867 93 21
BmrI ACTGGGnnnn_n'
Cuts at: 889 970 1305 3449 889
Size: 81 335 2144 1117
Fragments arranged by size:
2144 1117 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 3677
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 853 1732 2224 2522 853
Size: 879 492 298 2008
Fragments arranged by size:
2008 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3381 3381
Size: 3677
BsaAI yAC'GTr
Cuts at: 1030 1030
Size: 3677
BsaBI GATnn'nnATC
Cuts at: 6 1191 3228 6
Size: 1185 2037 455
Fragments arranged by size:
2037 1185 455
BsaHI Gr'CG_yC
Cuts at: 1108 1356 2058 3554 1108
Size: 248 702 1496 1231
Fragments arranged by size:
1496 1231 702 248
BsaWI w'CCGG_w
Cuts at: 308 526 866 1387 2065 2339 2486 2897 308
Size: 218 340 521 678 274 147 411 1088
Fragments arranged by size:
1088 678 521 411 340 274 218 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 595 625 595
Size: 30 3647
BseYI C'CCAG_C
Cuts at: 1102 1860 2437 2933 1102
Size: 758 577 496 1846
Fragments arranged by size:
1846 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 283 347 1219 1265 2473 104
Size: 179 64 872 46 1208 1308
Fragments arranged by size:
1308 1208 872 179 64 46
BsiHKAI G_wGCw'C
Cuts at: 414 691 1469 1659 2451 414
Size: 277 778 190 792 1640
Fragments arranged by size:
1640 792 778 277 190
Bsp1286I G_dGCh'C
Cuts at: 161 414 435 691 1302 1395 1469 1659 1721
Size: 253 21 256 611 93 74 190 62
Cuts at: 1721 2451 161
Size: 730 1387
Fragments arranged by size:
1387 730 611 256 253 190 93 74
62 21
BspEI T'CCGG_A
Cuts at: 308 2897 308
Size: 2589 1088
BspHI T'CATG_A
Cuts at: 2853 2853
Size: 3677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 727 941 1243 1624 727
Size: 214 302 381 2780
Fragments arranged by size:
2780 381 302 214
BsrBI CCG'CTC
Cuts at: 50 611 1969 2023 3507 3672 50
Size: 561 1358 54 1484 165 55
Fragments arranged by size:
1484 1358 561 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1589 1589
Size: 3677
BsrFI r'CCGG_y
Cuts at: 278 554 1675 1856 2065 278
Size: 276 1121 181 209 1890
Fragments arranged by size:
1890 1121 276 209 181
BssSI C'ACGA_G
Cuts at: 1948 2306 1948
Size: 358 3319
BstAPI GCAn_nnn'nTGC
Cuts at: 1054 1054
Size: 3677
BstBI TT'CG_AA
Cuts at: 2038 2038
Size: 3677
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 3677
BstYI r'GATC_y
Cuts at: 1 464 702 1169 1192 1527 1773 2785 2871
Size: 463 238 467 23 335 246 1012 86
Cuts at: 2871 2883 3229 1
Size: 12 346 449
Fragments arranged by size:
1012 467 463 449 346 335 246 238
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 181 448 500 181
Size: 267 52 3358
Fragments arranged by size:
3358 267 52
BtgI C'CryG_G
Cuts at: 107 347 107
Size: 240 3437
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1627 1808 1971 1627
Size: 181 163 3333
Fragments arranged by size:
3333 181 163
BtsI GCAGTG_nn'
Cuts at: 412 920 412
Size: 508 3169
DraI TTT'AAA
Cuts at: 676 676
Size: 3677
DrdI GACnn_nn'nnGTC
Cuts at: 1383 2241 1383
Size: 858 2819
EaeI y'GGCC_r
Cuts at: 212 280 327 344 1262 1436 1827 1854 3199
Size: 68 47 17 918 174 391 27 1345
Cuts at: 3199 212
Size: 690
Fragments arranged by size:
1345 918 690 391 174 68 47 27
17
EagI C'GGCC_G
Cuts at: 280 344 1262 280
Size: 64 918 2695
Fragments arranged by size:
2695 918 64
EarI CTCTTCn'nnn_
Cuts at: 247 1700 1910 247
Size: 1453 210 2014
Fragments arranged by size:
2014 1453 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 112 1885 2195 2341 3487 112
Size: 1773 310 146 1146 302
Fragments arranged by size:
1773 1146 310 302 146
EcoICRI GAG'CTC
Cuts at: 689 689
Size: 3677
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 421 1502 1934 2681 2949 421
Size: 1081 432 747 268 1149
Fragments arranged by size:
1149 1081 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 570 816 570
Size: 246 3431
EcoRI G'AATT_C
Cuts at: 681 681
Size: 3677
EcoRV GAT'ATC
Cuts at: 174 174
Size: 3677
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1696 1696
Size: 3677
FspI TGC'GCA
Cuts at: 1458 1458
Size: 3677
HaeII r_GCGC'y
Cuts at: 1111 1359 2381 3360 1111
Size: 248 1022 979 1428
Fragments arranged by size:
1428 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 104 136 1519 1549 1551 1581 104
Size: 32 1383 30 2 30 2200
Fragments arranged by size:
2200 1383 32 30 30 2
HincII GTy'rAC
Cuts at: 716 716
Size: 3677
HindIII A'AGCT_T
Cuts at: 319 319
Size: 3677
KasI G'GCGC_C
Cuts at: 1107 1355 1107
Size: 248 3429
KpnI G_GTAC'C
Cuts at: 394 697 394
Size: 303 3374
MluI A'CGCG_T
Cuts at: 3110 3110
Size: 3677
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2348 2532 2348
Size: 184 3493
MscI TGG'CCA
Cuts at: 214 1438 214
Size: 1224 2453
MslI CAynn'nnrTG
Cuts at: 112 1666 1793 112
Size: 1554 127 1996
Fragments arranged by size:
1996 1554 127
MspA1I CmG'CkG
Cuts at: 483 788 1102 1462 2475 2720 483
Size: 305 314 360 1013 245 1440
Fragments arranged by size:
1440 1013 360 314 305 245
NaeI GCC'GGC
Cuts at: 1858 1858
Size: 3677
NarI GG'CG_CC
Cuts at: 1108 1356 1108
Size: 248 3429
NcoI C'CATG_G
Cuts at: 107 107
Size: 3677
NgoMIV G'CCGG_C
Cuts at: 1856 1856
Size: 3677
NspI r_CATG'y
Cuts at: 730 1230 2137 730
Size: 500 907 2270
Fragments arranged by size:
2270 907 500
PciI A'CATG_T
Cuts at: 2133 2133
Size: 3677
PfoI T'CCnGG_A
Cuts at: 298 298
Size: 3677
PmeI GTTT'AAAC
Cuts at: 676 676
Size: 3677
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2816 2848 2816
Size: 32 3645
PspOMI G'GGCC_C
Cuts at: 431 431
Size: 3677
PstI C_TGCA'G
Cuts at: 724 1409 724
Size: 685 2992
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3677
PvuII CAG'CTG
Cuts at: 483 1102 1462 483
Size: 619 360 2698
Fragments arranged by size:
2698 619 360
RsrII CG'GwC_CG
Cuts at: 1872 1872
Size: 3677
SacI G_AGCT'C
Cuts at: 691 691
Size: 3677
SalI G'TCGA_C
Cuts at: 714 714
Size: 3677
SapI GCTCTTCn'nnn_
Cuts at: 1700 1910 1700
Size: 210 3467
SbfI CC_TGCA'GG
Cuts at: 724 724
Size: 3677
ScaI AGT'ACT
Cuts at: 3194 3194
Size: 3677
SexAI A'CCwGG_T
Cuts at: 239 239
Size: 3677
SfcI C'TryA_G
Cuts at: 33 720 1405 2398 2589 33
Size: 687 685 993 191 1121
Fragments arranged by size:
1121 993 687 685 191
SfoI GGC'GCC
Cuts at: 1109 1357 1109
Size: 248 3429
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3677
SmaI CCC'GGG
Cuts at: 699 699
Size: 3677
SmlI C'TyrA_G
Cuts at: 832 1747 2239 2501 832
Size: 915 492 262 2008
Fragments arranged by size:
2008 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3677
SphI G_CATG'C
Cuts at: 730 1230 730
Size: 500 3177
StyI C'CwwG_G
Cuts at: 107 614 811 1165 107
Size: 507 197 354 2619
Fragments arranged by size:
2619 507 354 197
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 152 1260 1393 1852 2061 152
Size: 1108 133 459 209 1768
Fragments arranged by size:
1768 1108 459 209 133
TatI w'GTAC_w
Cuts at: 3192 3192
Size: 3677
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3194 3226 3194
Size: 32 3645
Tth111I GACn'n_nGTC
Cuts at: 1474 1474
Size: 3677
XbaI T'CTAG_A
Cuts at: 708 708
Size: 3677
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1172 1172
Size: 3677
XmaI C'CCGG_G
Cuts at: 697 697
Size: 3677
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PfoI PmeI PpiI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BglI BmgBI
BmtI BpmI Bpu10I BseRI BsgI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SrfI
SspI StuI SwaI XhoI XmnI ZraI