Restriction map for pF1KB3336
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

Acc65I      AccI        AccII       AccIII      AclI        
AcsI        AflIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AviII       
AvrII       BamHI       BbeI        BcgI        BciVI       
BlnI        BsaWI       BseAI       BsiEI       BsiWI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrGI       BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     CfoI        ClaI        
CpoI        DraI        DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FseI        FspI        HaeII       
HhaI        HinP1I      HindIII     HpaI        KasI        
KpnI        KspI        MfeI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        Tsp509I     XbaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AatII         895  GACGTC               GACGT^C
AflII        1044  CTTAAG               C^TTAAG
AocI          315  CCTGAGG              CC^T[ACGT]AGG
ApaI         1568  GGGCCC               GGGCC^C
AspHI         876  GAGCTC               G[AT]GC[AT]^C
AvaI          117  CTCGAG               C^[CT]CG[GA]G
BalI          356  TGGCCA               TGG^CCA
BbrPI         424  CACGTG               CAC^GTG
BbvCI         750  CCTCAGC              CCTCAGC(-5/-2)
BclI          791  TGATCA               T^GATCA
BfaI          973  CTAG                 C^TAG
BfrI         1044  CTTAAG               C^TTAAG
BglII         962  AGATCT               A^GATCT
BlpI          873  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      873  GCTGAGC              GC^T[ACGT]AGC
BsaAI         424  CACGTG               [CT]AC^GT[GA]
BsaBI         354  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          430  GAGACC               GGTCTC(1/5)
BsgI         1254  CTGCAC               GTGCAG(16/14)
BsiHKAI       876  GAGCTC               G[AT]GC[AT]^C
BsoBI         117  CTCGAG               C^[CT]CG[GA]G
BspMI        1277  ACCTGC               ACCTGC(4/8)
BsrFI        1348  GCCGGT               [GA]^CCGG[CT]
BssSI         742  CTCGTG               CACGAG(-5/-1)
Bsu36I        315  CCTGAGG              CC^T[ACGT]AGG
CelII         873  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       1348  GCCGGT               [GA]^CCGG[CT]
DraIII       1645  CACTTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        315  CCTGAGG              CC^T[ACGT]AGG
FbaI          791  TGATCA               T^GATCA
HincII        301  GTTGAC               GT[CT]^[GA]AC
HindII        301  GTTGAC               GT[CT]^[GA]AC
MaeI          973  CTAG                 C^TAG
MamI          354  GATGGCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI         356  TGGCCA               TGG^CCA
MscI          356  TGGCCA               TGG^CCA
MslI          774  CATCTTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI          814  CCATGG               C^CATGG
NspI          103  ACATGC               [GA]CATG^[CT]
PaeR7I        117  CTCGAG               C^TCGAG
PmaCI         424  CACGTG               CAC^GTG
PmlI          424  CACGTG               CAC^GTG
SacI          876  GAGCTC               GAGCT^C
SapI         1284  GCTCTTC              GCTCTTC(1/4)
SexAI        1442  ACCTGGT              A^CC[AT]GGT
TfiI          280  GATTC                G^A[AT]TC
XcmI          520  CCAGGCCACACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          117  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI          783  GTAC                 GT^AC
             1140  GTAC                
AlwI          264  GATCC                GGATC(4/5)
              575  GGATC               
AspI          304  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              510  GACGCTGTC           
BbsI         1168  GAAGAC               GAAGAC(2/6)
             1206  GTCTTC              
BpuAI        1168  GAAGAC               GAAGAC(2/6)
             1206  GTCTTC              
BsmBI         586  GAGACG               CGTCTC(1/5)
              893  GAGACG              
BstYI         575  GGATCT               [GA]^GATC[CT]
              962  AGATCT              
DsaI          724  CCGTGG               C^C[GA][CT]GG
              814  CCATGG              
MaeII         425  ACGT                 A^CGT
              896  ACGT                
MflI          575  GGATCT               [GA]^GATC[CT]
              962  AGATCT              
MseI         1045  TTAA                 T^TAA
             1574  TTAA                
PflFI         304  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              510  GACGCTGTC           
RsaI          783  GTAC                 GT^AC
             1140  GTAC                
StuI         1127  AGGCCT               AGG^CCT
             1543  AGGCCT              
Tru9I        1045  TTAA                 T^TAA
             1574  TTAA                
Tsp45I        310  GTCAC                ^GT[GC]AC
              997  GTGAC               
Tth111I       304  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              510  GACGCTGTC           
XhoII         575  GGATCT               [GA]^GATC[CT]
              962  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AcyI        AluI        AlwNI       AvaII       
BanI        BanII       BbvI        BcnI        BglI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HgaI        Hin1I       HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeIII      MboI        MboII       
MnlI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PpuMI       PstI        PvuII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TseI        TspRI       TthHB8I     
Van91I      
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com