Restriction map for
pF1KB3336
(Circular) (Six-base) MAPSORT of: pF1KB3336.seq Check: 2934 from: 1 to: 4766
pF1KB3336 4766 bp
With 182 enzymes: SgfI *
June 20, 2008 10:23 ..
AatII G_ACGT'C
Cuts at: 1007 1007
Size: 4766
AccI GT'mk_AC
Cuts at: 1804 1804
Size: 4766
Acc65I G'GTAC_C
Cuts at: 1782 1782
Size: 4766
AclI AA'CG_TT
Cuts at: 4606 4606
Size: 4766
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 777 949 961 1029 2591 3023 3376 4038 777
Size: 172 12 68 1562 432 353 662 1505
Fragments arranged by size:
1562 1505 662 432 353 172 68 12
AflII C'TTAA_G
Cuts at: 1152 1152
Size: 4766
AflIII A'CryG_T
Cuts at: 2118 3918 4199 2118
Size: 1800 281 2685
Fragments arranged by size:
2685 1800 281
AlwNI CAG_nnn'CTG
Cuts at: 399 786 1338 1505 1521 2071 3509 399
Size: 387 552 167 16 550 1438 1656
Fragments arranged by size:
1656 1438 552 550 387 167 16
ApaI G_GGCC'C
Cuts at: 1680 1680
Size: 4766
ApaLI G'TGCA_C
Cuts at: 3604 3604
Size: 4766
ApoI r'AATT_y
Cuts at: 1770 4119 4375 1770
Size: 2349 256 2161
Fragments arranged by size:
2349 2161 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4766
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
AvaI C'yCGr_G
Cuts at: 225 1786 225
Size: 1561 3205
BamHI G'GATC_C
Cuts at: 1791 1791
Size: 4766
BanI G'GyrC_C
Cuts at: 1062 1134 1302 1782 2196 2444 2479 1062
Size: 72 168 480 414 248 35 3349
Fragments arranged by size:
3349 480 414 248 168 72 35
BanII G_rGCy'C
Cuts at: 267 384 772 988 1145 1680 1780 2810 267
Size: 117 388 216 157 535 100 1030 2223
Fragments arranged by size:
2223 1030 535 388 216 157 117 100
BbeI G_GCGC'C
Cuts at: 2200 2448 2200
Size: 248 4518
BbsI GAAGACnn'nnnn_
Cuts at: 1283 1307 1283
Size: 24 4742
BbvCI CC'TCA_GC
Cuts at: 859 859
Size: 4766
BciVI GTATCCnnnnn_n'
Cuts at: 2664 3720 4766 2664
Size: 1056 1046 2664
Fragments arranged by size:
2664 1056 1046
BclI T'GATC_A
Cuts at: 899 2286 899
Size: 1387 3379
BglI GCCn_nnn'nGGC
Cuts at: 112 1055 1524 1659 112
Size: 943 469 135 3219
Fragments arranged by size:
3219 943 469 135
BglII A'GATC_T
Cuts at: 1070 2281 4318 1070
Size: 1211 2037 1518
Fragments arranged by size:
2037 1518 1211
BlpI GC'TnA_GC
Cuts at: 982 1878 982
Size: 896 3870
Bme1580I G_kGCm'C
Cuts at: 1305 1680 2391 2484 3608 1305
Size: 375 711 93 1124 2463
Fragments arranged by size:
2463 1124 711 375 93
BmrI ACTGGGnnnn_n'
Cuts at: 198 708 1397 1515 1978 2059 2394 4538 198
Size: 510 689 118 463 81 335 2144 426
Fragments arranged by size:
2144 689 510 463 426 335 118 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3133 3133
Size: 4766
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 622 712 786 795 1111 1359 1717 270
Size: 352 90 74 9 316 248 358 3319
Fragments arranged by size:
3319 358 352 316 248 90 74 9
Bpu10I CC'TnA_GC
Cuts at: 859 1139 1574 859
Size: 280 435 4051
Fragments arranged by size:
4051 435 280
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 649 1942 2821 3535 3833 649
Size: 1293 879 714 298 1582
Fragments arranged by size:
1582 1293 879 714 298
BsaI GGTCTCn'nnnn_
Cuts at: 532 4470 532
Size: 3938 828
BsaAI yAC'GTr
Cuts at: 534 2119 534
Size: 1585 3181
BsaBI GATnn'nnATC
Cuts at: 6 466 2280 4317 6
Size: 460 1814 2037 455
Fragments arranged by size:
2037 1814 460 455
BsaHI Gr'CG_yC
Cuts at: 132 1004 1355 2197 2445 3147 4643 132
Size: 872 351 842 248 702 1496 255
Fragments arranged by size:
1496 872 842 702 351 255 248
BsaWI w'CCGG_w
Cuts at: 1955 2476 3154 3565 3712 3986 1955
Size: 521 678 411 147 274 2735
Fragments arranged by size:
2735 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 989 1019 989
Size: 30 4736
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 133 142 658 838 1013 1399 1615 133
Size: 9 516 180 175 386 216 3284
Fragments arranged by size:
3284 516 386 216 180 175 9
BseYI C'CCAG_C
Cuts at: 560 977 1420 1514 1594 2191 2949 3614 4022
Size: 417 443 94 80 597 758 665 408
Cuts at: 4022 560
Size: 1304
Fragments arranged by size:
1304 758 665 597 443 417 408 94
80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1347 1347
Size: 4766
BsiEI CG_ry'CG
Cuts at: 104 2308 2354 3584 104
Size: 2204 46 1230 1286
Fragments arranged by size:
2204 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 988 1780 2558 2748 3608 988
Size: 792 778 190 860 2146
Fragments arranged by size:
2146 860 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 688 995 688
Size: 307 4459
Bsp1286I G_dGCh'C
Cuts at: 267 384 772 988 1145 1305 1680 1780 2391
Size: 117 388 216 157 160 375 100 611
Cuts at: 2391 2484 2558 2748 2810 3608 267
Size: 93 74 190 62 798 1425
Fragments arranged by size:
1425 798 611 388 375 216 190 160
157 117 100 93 74 62
BspHI T'CATG_A
Cuts at: 3198 3198
Size: 4766
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1394 1816 2030 2332 2713 1394
Size: 422 214 302 381 3447
Fragments arranged by size:
3447 422 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3058 3112 4596 4761 50
Size: 3008 54 1484 165 55
Fragments arranged by size:
3008 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2678 2678
Size: 4766
BsrFI r'CCGG_y
Cuts at: 1456 2764 2945 1456
Size: 1308 181 3277
Fragments arranged by size:
3277 1308 181
BssSI C'ACGA_G
Cuts at: 850 3037 3745 850
Size: 2187 708 1871
Fragments arranged by size:
2187 1871 708
BstAPI GCAn_nnn'nTGC
Cuts at: 2143 2143
Size: 4766
BstBI TT'CG_AA
Cuts at: 3127 3127
Size: 4766
BstYI r'GATC_y
Cuts at: 1 683 1070 1791 2258 2281 2616 2862 3168
Size: 682 387 721 467 23 335 246 306
Cuts at: 3168 3180 3266 4318 1
Size: 12 86 1052 449
Fragments arranged by size:
1052 721 682 467 449 387 335 306
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 424 424
Size: 4766
BtgI C'CryG_G
Cuts at: 107 832 922 107
Size: 725 90 3951
Fragments arranged by size:
3951 725 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 465 2716 2897 3060 465
Size: 2251 181 163 2171
Fragments arranged by size:
2251 2171 181 163
BtsI GCAGTG_nn'
Cuts at: 1433 1455 2009 1433
Size: 22 554 4190
Fragments arranged by size:
4190 554 22
DraI TTT'AAA
Cuts at: 1765 1765
Size: 4766
DraIII CAC_nnn'GTG
Cuts at: 1758 1758
Size: 4766
DrdI GACnn_nn'nnGTC
Cuts at: 2472 3816 2472
Size: 1344 3422
EaeI y'GGCC_r
Cuts at: 298 464 840 1295 2351 2525 2916 2943 4288
Size: 166 376 455 1056 174 391 27 1345
Cuts at: 4288 298
Size: 776
Fragments arranged by size:
1345 1056 776 455 391 376 174 166
27
EagI C'GGCC_G
Cuts at: 2351 2351
Size: 4766
EarI CTCTTCn'nnn_
Cuts at: 1026 1399 1457 2789 2999 1026
Size: 373 58 1332 210 2793
Fragments arranged by size:
2793 1332 373 210 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2974 3716 3862 4576 2974
Size: 742 146 714 3164
Fragments arranged by size:
3164 742 714 146
EcoICRI GAG'CTC
Cuts at: 986 1778 986
Size: 792 3974
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 270 622 712 777 786 795 949 961 1029
Size: 352 90 65 9 9 154 12 68
Cuts at: 1029 1111 1359 1717 2591 3023 3376 4038 270
Size: 82 248 358 874 432 353 662 998
Fragments arranged by size:
998 874 662 432 358 353 352 248
154 90 82 68 65 12 9 9
EcoNI CCTnn'n_nnAGG
Cuts at: 746 817 1123 1340 1647 746
Size: 71 306 217 307 3865
Fragments arranged by size:
3865 307 306 217 71
EcoO109I rG'GnC_Cy
Cuts at: 648 936 1509 1525 1605 1676 1677 1743 1905
Size: 288 573 16 80 71 1 66 162
Cuts at: 1905 648
Size: 3509
Fragments arranged by size:
3509 573 288 162 80 71 66 16
1
EcoRI G'AATT_C
Cuts at: 1770 1770
Size: 4766
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2785 2785
Size: 4766
FspI TGC'GCA
Cuts at: 2547 2547
Size: 4766
HaeII r_GCGC'y
Cuts at: 2200 2448 3678 4449 2200
Size: 248 1230 771 2517
Fragments arranged by size:
2517 1230 771 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 221 253 989 1021 1132 1164 1252 1284 2608
Size: 32 736 32 111 32 88 32 1324
Cuts at: 2608 2638 2640 2670 221
Size: 30 2 30 2317
Fragments arranged by size:
2317 1324 736 111 88 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 411 1805 411
Size: 1394 3372
KasI G'GCGC_C
Cuts at: 2196 2444 2196
Size: 248 4518
KpnI G_GTAC'C
Cuts at: 1786 1786
Size: 4766
MluI A'CGCG_T
Cuts at: 4199 4199
Size: 4766
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3525 3709 3525
Size: 184 4582
MscI TGG'CCA
Cuts at: 466 2527 466
Size: 2061 2705
MslI CAynn'nnrTG
Cuts at: 886 2755 2882 886
Size: 1869 127 2770
Fragments arranged by size:
2770 1869 127
MspA1I CmG'CkG
Cuts at: 187 863 981 1113 1178 1877 2191 2551 3335
Size: 676 118 132 65 699 314 360 784
Cuts at: 3335 3580 187
Size: 245 1373
Fragments arranged by size:
1373 784 699 676 360 314 245 132
118 65
NaeI GCC'GGC
Cuts at: 2947 2947
Size: 4766
NarI GG'CG_CC
Cuts at: 2197 2445 2197
Size: 248 4518
NcoI C'CATG_G
Cuts at: 107 922 107
Size: 815 3951
NgoMIV G'CCGG_C
Cuts at: 2945 2945
Size: 4766
NspI r_CATG'y
Cuts at: 215 1819 2319 3922 215
Size: 1604 500 1603 1059
Fragments arranged by size:
1604 1603 1059 500
PasI CC'CwG_GG
Cuts at: 1441 1521 1622 1441
Size: 80 101 4585
Fragments arranged by size:
4585 101 80
PciI A'CATG_T
Cuts at: 3918 3918
Size: 4766
PflMI CCAn_nnn'nTGG
Cuts at: 786 833 1505 1521 786
Size: 47 672 16 4031
Fragments arranged by size:
4031 672 47 16
PfoI T'CCnGG_A
Cuts at: 470 1214 470
Size: 744 4022
PmeI GTTT'AAAC
Cuts at: 1765 1765
Size: 4766
PmlI CAC'GTG
Cuts at: 534 534
Size: 4766
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3212 3244 3212
Size: 32 4734
PpuMI rG'GwC_Cy
Cuts at: 936 1509 1605 1743 936
Size: 573 96 138 3959
Fragments arranged by size:
3959 573 138 96
PspOMI G'GGCC_C
Cuts at: 1676 1676
Size: 4766
PstI C_TGCA'G
Cuts at: 186 272 810 910 1124 1334 1813 2498 186
Size: 86 538 100 214 210 479 685 2454
Fragments arranged by size:
2454 685 538 479 214 210 100 86
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4766
PvuII CAG'CTG
Cuts at: 981 1113 1178 2191 2551 981
Size: 132 65 1013 360 3196
Fragments arranged by size:
3196 1013 360 132 65
RsrII CG'GwC_CG
Cuts at: 2961 2961
Size: 4766
SacI G_AGCT'C
Cuts at: 988 1780 988
Size: 792 3974
SalI G'TCGA_C
Cuts at: 1803 1803
Size: 4766
SanDI GG'GwC_CC
Cuts at: 1509 1509
Size: 4766
SapI GCTCTTCn'nnn_
Cuts at: 1399 2789 2999 1399
Size: 1390 210 3166
Fragments arranged by size:
3166 1390 210
SbfI CC_TGCA'GG
Cuts at: 1813 1813
Size: 4766
ScaI AGT'ACT
Cuts at: 4283 4283
Size: 4766
SexAI A'CCwGG_T
Cuts at: 1550 1550
Size: 4766
SfcI C'TryA_G
Cuts at: 33 182 268 806 906 1120 1330 1809 2494
Size: 149 86 538 100 214 210 479 685
Cuts at: 2494 3462 3653 33
Size: 968 191 1146
Fragments arranged by size:
1146 968 685 538 479 214 210 191
149 100 86
SfoI GGC'GCC
Cuts at: 2198 2446 2198
Size: 248 4518
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4766
SmaI CCC'GGG
Cuts at: 1788 1788
Size: 4766
SmlI C'TyrA_G
Cuts at: 225 664 1152 1921 2836 3550 3812 225
Size: 439 488 769 915 714 262 1179
Fragments arranged by size:
1179 915 769 714 488 439 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4766
SphI G_CATG'C
Cuts at: 1819 2319 1819
Size: 500 4266
StuI AGG'CCT
Cuts at: 1237 1653 1237
Size: 416 4350
StyI C'CwwG_G
Cuts at: 107 813 922 1223 1900 2254 107
Size: 706 109 301 677 354 2619
Fragments arranged by size:
2619 706 677 354 301 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 727 1320 2349 2482 2941 3150 727
Size: 593 1029 133 459 209 2343
Fragments arranged by size:
2343 1029 593 459 209 133
TatI w'GTAC_w
Cuts at: 890 1247 4281 890
Size: 357 3034 1375
Fragments arranged by size:
3034 1375 357
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4283 4315 4283
Size: 32 4734
Tth111I GACn'n_nGTC
Cuts at: 415 621 2563 415
Size: 206 1942 2618
Fragments arranged by size:
2618 1942 206
XbaI T'CTAG_A
Cuts at: 1797 1797
Size: 4766
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 635 2261 635
Size: 1626 3140
XhoI C'TCGA_G
Cuts at: 225 225
Size: 4766
XmaI C'CCGG_G
Cuts at: 1786 1786
Size: 4766
ZraI GAC'GTC
Cuts at: 1005 1005
Size: 4766
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AarI AfeI AgeI AhdI AleI AlfI AloI AscI
AvrII BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmI
BspEI BsrGI BssHII BstEII BstXI BstZ17I ClaI CspCI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PshAI PsiI PspXI PsrI
SacII SfiI SgrAI SnaBI SrfI SspI SwaI XmnI