Restriction map for
pF1KB1529
(Circular) (Six-base) MAPSORT of: pF1KB1529.seq Check: 5352 from: 1 to: 7019
pF1KB1529 7019 bp
With 182 enzymes: SgfI *
June 20, 2008 10:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 193 193
Size: 7019
AccI GT'mk_AC
Cuts at: 2332 4057 2332
Size: 1725 5294
Acc65I G'GTAC_C
Cuts at: 871 4035 871
Size: 3164 3855
AclI AA'CG_TT
Cuts at: 6859 6859
Size: 7019
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 797 884 1310 2334 2396 2612 3733 4844 5276
Size: 87 426 1024 62 216 1121 1111 432
Cuts at: 5276 5629 6291 797
Size: 353 662 1525
Fragments arranged by size:
1525 1121 1111 1024 662 432 426 353
216 87 62
AflII C'TTAA_G
Cuts at: 2647 2647
Size: 7019
AflIII A'CryG_T
Cuts at: 3115 4371 6171 6452 3115
Size: 1256 1800 281 3682
Fragments arranged by size:
3682 1800 1256 281
AgeI A'CCGG_T
Cuts at: 1299 1299
Size: 7019
AhdI GACnn_n'nnGTC
Cuts at: 1366 1366
Size: 7019
AleI CACnn'nnGTG
Cuts at: 3682 3682
Size: 7019
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2723 2723
Size: 7019
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 700 732 700
Size: 32 6987
AlwNI CAG_nnn'CTG
Cuts at: 127 1333 1804 2059 3084 3711 4324 5762 127
Size: 1206 471 255 1025 627 613 1438 1384
Fragments arranged by size:
1438 1384 1206 1025 627 613 471 255
ApaLI G'TGCA_C
Cuts at: 5857 5857
Size: 7019
ApoI r'AATT_y
Cuts at: 630 1786 2122 2455 3214 4023 6372 6628 630
Size: 1156 336 333 759 809 2349 256 1021
Fragments arranged by size:
2349 1156 1021 809 759 336 333 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 7019
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 7019
AvaI C'yCGr_G
Cuts at: 3873 4039 3873
Size: 166 6853
AvrII C'CTAG_G
Cuts at: 393 795 393
Size: 402 6617
BamHI G'GATC_C
Cuts at: 954 1510 2860 4044 954
Size: 556 1350 1184 3929
Fragments arranged by size:
3929 1350 1184 556
BanI G'GyrC_C
Cuts at: 871 3139 3929 4035 4449 4697 4732 871
Size: 2268 790 106 414 248 35 3158
Fragments arranged by size:
3158 2268 790 414 248 106 35
BanII G_rGCy'C
Cuts at: 1342 4033 5063 1342
Size: 2691 1030 3298
Fragments arranged by size:
3298 2691 1030
BbeI G_GCGC'C
Cuts at: 4453 4701 4453
Size: 248 6771
BbsI GAAGACnn'nnnn_
Cuts at: 2340 2396 2528 2340
Size: 56 132 6831
Fragments arranged by size:
6831 132 56
BbvCI CC'TCA_GC
Cuts at: 1351 2868 1351
Size: 1517 5502
BciVI GTATCCnnnnn_n'
Cuts at: 830 1082 4917 5973 7019 830
Size: 252 3835 1056 1046 830
Fragments arranged by size:
3835 1056 1046 830 252
BclI T'GATC_A
Cuts at: 3269 4539 3269
Size: 1270 5749
BfrBI ATG'CAT
Cuts at: 3490 3490
Size: 7019
BglII A'GATC_T
Cuts at: 1792 2547 2643 4534 6571 1792
Size: 755 96 1891 2037 2240
Fragments arranged by size:
2240 2037 1891 755 96
BlpI GC'TnA_GC
Cuts at: 962 4131 962
Size: 3169 3850
Bme1580I G_kGCm'C
Cuts at: 1593 3522 3932 4644 4737 5861 1593
Size: 1929 410 712 93 1124 2751
Fragments arranged by size:
2751 1929 1124 712 410 93
BmrI ACTGGGnnnn_n'
Cuts at: 818 4231 4312 4647 6791 818
Size: 3413 81 335 2144 1046
Fragments arranged by size:
3413 2144 1046 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3874 5386 3874
Size: 1512 5507
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 428 656 1724 2433 3353 3685 410
Size: 18 228 1068 709 920 332 3744
Fragments arranged by size:
3744 1068 920 709 332 228 18
Bpu10I CC'TnA_GC
Cuts at: 1128 1351 2868 3833 1128
Size: 223 1517 965 4314
Fragments arranged by size:
4314 1517 965 223
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 206 3131 3587 4195 5074 5788 6086 206
Size: 2925 456 608 879 714 298 1139
Fragments arranged by size:
2925 1139 879 714 608 456 298
BsaI GGTCTCn'nnnn_
Cuts at: 270 2974 3386 6723 270
Size: 2704 412 3337 566
Fragments arranged by size:
3337 2704 566 412
BsaAI yAC'GTr
Cuts at: 3116 4372 3116
Size: 1256 5763
BsaBI GATnn'nnATC
Cuts at: 6 4533 6570 6
Size: 4527 2037 455
Fragments arranged by size:
4527 2037 455
BsaHI Gr'CG_yC
Cuts at: 528 4450 4698 5400 6896 528
Size: 3922 248 702 1496 651
Fragments arranged by size:
3922 1496 702 651 248
BsaWI w'CCGG_w
Cuts at: 1299 4208 4729 5407 5818 5965 6239 1299
Size: 2909 521 678 411 147 274 2079
Fragments arranged by size:
2909 2079 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 700 730 1026 1056 2431 2461 3100 3130 700
Size: 30 296 30 1375 30 639 30 4589
Fragments arranged by size:
4589 1375 639 296 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 724 2654 3336 3891 724
Size: 1930 682 555 3852
Fragments arranged by size:
3852 1930 682 555
BseYI C'CCAG_C
Cuts at: 1248 1574 2991 3121 3126 3663 4444 5202 5867
Size: 326 1417 130 5 537 781 758 665
Cuts at: 5867 6275 1248
Size: 408 1992
Fragments arranged by size:
1992 1417 781 758 665 537 408 326
130 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 3708 3708
Size: 7019
BsiEI CG_ry'CG
Cuts at: 104 4561 4607 5837 104
Size: 4457 46 1230 1286
Fragments arranged by size:
4457 1286 1230 46
BsiHKAI G_wGCw'C
Cuts at: 2776 4033 4811 5001 5861 2776
Size: 1257 778 190 860 3934
Fragments arranged by size:
3934 1257 860 778 190
BsmI GAATG_Cn'
Cuts at: 389 977 3886 389
Size: 588 2909 3522
Fragments arranged by size:
3522 2909 588
Bsp1286I G_dGCh'C
Cuts at: 1342 1593 2776 3522 3932 4033 4644 4737 4811
Size: 251 1183 746 410 101 611 93 74
Cuts at: 4811 5001 5063 5861 1342
Size: 190 62 798 2500
Fragments arranged by size:
2500 1183 798 746 611 410 251 190
101 93 74 62
BspHI T'CATG_A
Cuts at: 5451 5451
Size: 7019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 193 3092 4016 4069 4283 4585 4966 193
Size: 2899 924 53 214 302 381 2246
Fragments arranged by size:
2899 2246 924 381 302 214 53
BsrBI CCG'CTC
Cuts at: 50 5311 5365 6849 7014 50
Size: 5261 54 1484 165 55
Fragments arranged by size:
5261 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1226 1627 4931 1226
Size: 401 3304 3314
Fragments arranged by size:
3314 3304 401
BsrFI r'CCGG_y
Cuts at: 1299 5017 5198 1299
Size: 3718 181 3120
Fragments arranged by size:
3718 3120 181
BsrGI T'GTAC_A
Cuts at: 3638 3638
Size: 7019
BssSI C'ACGA_G
Cuts at: 5290 5998 5290
Size: 708 6311
BstAPI GCAn_nnn'nTGC
Cuts at: 4396 4396
Size: 7019
BstBI TT'CG_AA
Cuts at: 5380 5380
Size: 7019
BstEII G'GTnAC_C
Cuts at: 3645 3645
Size: 7019
BstXI CCAn_nnnn'nTGG
Cuts at: 831 2289 3657 831
Size: 1458 1368 4193
Fragments arranged by size:
4193 1458 1368
BstYI r'GATC_y
Cuts at: 1 954 1510 1707 1792 2547 2643 2860 4044
Size: 953 556 197 85 755 96 217 1184
Cuts at: 4044 4511 4534 4869 5115 5421 5433 5519 6571
Size: 467 23 335 246 306 12 86 1052
Cuts at: 6571 1
Size: 449
Fragments arranged by size:
1184 1052 953 755 556 467 449 335
306 246 217 197 96 86 85 23
12
Bsu36I CC'TnA_GG
Cuts at: 1896 1896
Size: 7019
BtgI C'CryG_G
Cuts at: 107 149 107
Size: 42 6977
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 662 4969 5150 5313 662
Size: 4307 181 163 2368
Fragments arranged by size:
4307 2368 181 163
BtsI GCAGTG_nn'
Cuts at: 576 878 2504 2577 2774 3677 3689 3696 4262
Size: 302 1626 73 197 903 12 7 566
Cuts at: 4262 576
Size: 3333
Fragments arranged by size:
3333 1626 903 566 302 197 73 12
7
ClaI AT'CG_AT
Cuts at: 3722 3722
Size: 7019
DraI TTT'AAA
Cuts at: 1238 1659 1827 3009 4018 1238
Size: 421 168 1182 1009 4239
Fragments arranged by size:
4239 1182 1009 421 168
DrdI GACnn_nn'nnGTC
Cuts at: 2328 4725 6069 2328
Size: 2397 1344 3278
Fragments arranged by size:
3278 2397 1344
EaeI y'GGCC_r
Cuts at: 118 783 833 2694 4604 4778 5169 5196 6541
Size: 665 50 1861 1910 174 391 27 1345
Cuts at: 6541 118
Size: 596
Fragments arranged by size:
1910 1861 1345 665 596 391 174 50
27
EagI C'GGCC_G
Cuts at: 4604 4604
Size: 7019
EarI CTCTTCn'nnn_
Cuts at: 464 533 589 917 2978 5042 5252 464
Size: 69 56 328 2061 2064 210 2231
Fragments arranged by size:
2231 2064 2061 328 210 69 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 159 5227 5969 6115 6829 159
Size: 5068 742 146 714 349
Fragments arranged by size:
5068 742 714 349 146
EcoICRI GAG'CTC
Cuts at: 4031 4031
Size: 7019
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 410 428 656 797 884 1310 1724 2334 2396
Size: 18 228 141 87 426 414 610 62
Cuts at: 2396 2433 2612 3353 3685 3733 4844 5276 5629
Size: 37 179 741 332 48 1111 432 353
Cuts at: 5629 6291 410
Size: 662 1138
Fragments arranged by size:
1138 1111 741 662 610 432 426 414
353 332 228 179 141 87 62 48
37 18
EcoNI CCTnn'n_nnAGG
Cuts at: 200 224 392 2254 200
Size: 24 168 1862 4965
Fragments arranged by size:
4965 1862 168 24
EcoO109I rG'GnC_Cy
Cuts at: 331 697 1330 3081 4158 331
Size: 366 633 1751 1077 3192
Fragments arranged by size:
3192 1751 1077 633 366
EcoRI G'AATT_C
Cuts at: 1786 2122 4023 1786
Size: 336 1901 4782
Fragments arranged by size:
4782 1901 336
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1041 1186 2989 3374 5038 1041
Size: 145 1803 385 1664 3022
Fragments arranged by size:
3022 1803 1664 385 145
FspI TGC'GCA
Cuts at: 4800 4800
Size: 7019
HaeII r_GCGC'y
Cuts at: 115 4453 4701 5931 6702 115
Size: 4338 248 1230 771 432
Fragments arranged by size:
4338 1230 771 432 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1352 1384 1666 1698 1828 1860 2431 2463 2515
Size: 32 282 32 130 32 571 32 52
Cuts at: 2515 2547 2587 2619 3769 3784 3801 3816 4861
Size: 32 40 32 1150 15 17 15 1045
Cuts at: 4861 4891 4893 4923 1352
Size: 30 2 30 3448
Fragments arranged by size:
3448 1150 1045 571 282 130 52 40
32 32 32 32 32 32 30 30
17 15 15 2
HincII GTy'rAC
Cuts at: 2051 4058 2051
Size: 2007 5012
HindIII A'AGCT_T
Cuts at: 1776 1776
Size: 7019
KasI G'GCGC_C
Cuts at: 4449 4697 4449
Size: 248 6771
KpnI G_GTAC'C
Cuts at: 875 4039 875
Size: 3164 3855
MfeI C'AATT_G
Cuts at: 917 917
Size: 7019
MluI A'CGCG_T
Cuts at: 6452 6452
Size: 7019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1069 1288 1368 5778 5962 1069
Size: 219 80 4410 184 2126
Fragments arranged by size:
4410 2126 219 184 80
MscI TGG'CCA
Cuts at: 785 835 2696 4780 785
Size: 50 1861 2084 3024
Fragments arranged by size:
3024 2084 1861 50
MslI CAynn'nnrTG
Cuts at: 1081 2926 3495 3682 5008 5135 1081
Size: 1845 569 187 1326 127 2965
Fragments arranged by size:
2965 1845 1326 569 187 127
MspA1I CmG'CkG
Cuts at: 151 379 1672 2011 3947 4130 4444 4804 5588
Size: 228 1293 339 1936 183 314 360 784
Cuts at: 5588 5833 151
Size: 245 1337
Fragments arranged by size:
1936 1337 1293 784 360 339 314 245
228 183
NaeI GCC'GGC
Cuts at: 5200 5200
Size: 7019
NarI GG'CG_CC
Cuts at: 4450 4698 4450
Size: 248 6771
NcoI C'CATG_G
Cuts at: 107 107
Size: 7019
NdeI CA'TA_TG
Cuts at: 3492 3492
Size: 7019
NgoMIV G'CCGG_C
Cuts at: 5198 5198
Size: 7019
NsiI A_TGCA'T
Cuts at: 3492 3492
Size: 7019
NspI r_CATG'y
Cuts at: 420 453 2656 4072 4572 6175 420
Size: 33 2203 1416 500 1603 1264
Fragments arranged by size:
2203 1603 1416 1264 500 33
PasI CC'CwG_GG
Cuts at: 1100 2484 1100
Size: 1384 5635
PciI A'CATG_T
Cuts at: 6171 6171
Size: 7019
PflMI CCAn_nnn'nTGG
Cuts at: 127 1399 1615 3959 127
Size: 1272 216 2344 3187
Fragments arranged by size:
3187 2344 1272 216
PfoI T'CCnGG_A
Cuts at: 1481 1614 1481
Size: 133 6886
PmeI GTTT'AAAC
Cuts at: 4018 4018
Size: 7019
PmlI CAC'GTG
Cuts at: 3116 3116
Size: 7019
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 700 732 5465 5497 700
Size: 32 4733 32 2222
Fragments arranged by size:
4733 2222 32 32
PpuMI rG'GwC_Cy
Cuts at: 331 1330 3081 331
Size: 999 1751 4269
Fragments arranged by size:
4269 1751 999
PspXI vC'TCGA_Gb
Cuts at: 3873 3873
Size: 7019
PstI C_TGCA'G
Cuts at: 885 1410 1800 2715 3684 4066 4751 885
Size: 525 390 915 969 382 685 3153
Fragments arranged by size:
3153 969 915 685 525 390 382
PvuI CG_AT'CG
Cuts at: 104 104
Size: 7019
PvuII CAG'CTG
Cuts at: 1672 2011 3947 4444 4804 1672
Size: 339 1936 497 360 3887
Fragments arranged by size:
3887 1936 497 360 339
RsrII CG'GwC_CG
Cuts at: 5214 5214
Size: 7019
SacI G_AGCT'C
Cuts at: 4033 4033
Size: 7019
SacII CC_GC'GG
Cuts at: 152 152
Size: 7019
SalI G'TCGA_C
Cuts at: 4056 4056
Size: 7019
SapI GCTCTTCn'nnn_
Cuts at: 464 533 2978 5042 5252 464
Size: 69 2445 2064 210 2231
Fragments arranged by size:
2445 2231 2064 210 69
SbfI CC_TGCA'GG
Cuts at: 4066 4066
Size: 7019
ScaI AGT'ACT
Cuts at: 6536 6536
Size: 7019
SfcI C'TryA_G
Cuts at: 33 851 881 1406 1796 2711 2751 3680 4062
Size: 818 30 525 390 915 40 929 382
Cuts at: 4062 4747 5715 5906 33
Size: 685 968 191 1146
Fragments arranged by size:
1146 968 929 915 818 685 525 390
382 191 40 30
SfoI GGC'GCC
Cuts at: 4451 4699 4451
Size: 248 6771
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 7019
SgrAI Cr'CCGG_yG
Cuts at: 1299 1299
Size: 7019
SmaI CCC'GGG
Cuts at: 4041 4041
Size: 7019
SmlI C'TyrA_G
Cuts at: 221 2647 3146 3602 3873 4174 5089 5803 6065
Size: 2426 499 456 271 301 915 714 262
Cuts at: 6065 221
Size: 1175
Fragments arranged by size:
2426 1175 915 714 499 456 301 271
262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 7019
SphI G_CATG'C
Cuts at: 420 4072 4572 420
Size: 3652 500 2867
Fragments arranged by size:
3652 2867 500
SspI AAT'ATT
Cuts at: 2143 3616 2143
Size: 1473 5546
StuI AGG'CCT
Cuts at: 230 230
Size: 7019
StyI C'CwwG_G
Cuts at: 107 327 393 704 795 979 1356 3333 4153
Size: 220 66 311 91 184 377 1977 820
Cuts at: 4153 4507 107
Size: 354 2619
Fragments arranged by size:
2619 1977 820 377 354 311 220 184
91 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1387 4602 4735 5194 5403 1387
Size: 3215 133 459 209 3003
Fragments arranged by size:
3215 3003 459 209 133
TatI w'GTAC_w
Cuts at: 437 739 3446 3638 6534 437
Size: 302 2707 192 2896 922
Fragments arranged by size:
2896 2707 922 302 192
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1026 1058 1874 1906 3100 3132 3954 3986 6536
Size: 32 816 32 1194 32 822 32 2550
Cuts at: 6536 6568 1026
Size: 32 1477
Fragments arranged by size:
2550 1477 1194 822 816 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 4816 4816
Size: 7019
XbaI T'CTAG_A
Cuts at: 4050 4050
Size: 7019
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1420 4514 1420
Size: 3094 3925
XhoI C'TCGA_G
Cuts at: 3873 3873
Size: 7019
XmaI C'CCGG_G
Cuts at: 4039 4039
Size: 7019
XmnI GAAnn'nnTTC
Cuts at: 3590 3590
Size: 7019
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PspXI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI ApaI AscI BaeI BcgI BglI BmgBI
BmtI BsiWI BsmBI BspEI BssHII BstZ17I CspCI DraIII
EcoRV FseI FspAI HpaI NheI NotI NruI PacI
PshAI PsiI PspOMI PsrI SanDI SexAI SfiI SnaBI
SrfI SwaI ZraI