Restriction map for pF1KB1226
 (Linear) (Six-base) MAPSORT of: KIBB1226.seq  Check: 5234  from: 1  to: 3666



KIBB1226.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1383    3666 
   Size:       1383    2283
 
Acc65I G'GTAC_C

Cuts at:      0    2867    3666 
   Size:       2867     799
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289    2116    2869    3666 
   Size:        289    1827     753     797

  Fragments arranged by size: 

                1827     797     753     289
 
AflIII A'CryG_T

Cuts at:      0    1736    1955    3666 
   Size:       1736     219    1711

  Fragments arranged by size: 

                1736    1711     219
 
AgeI A'CCGG_T

Cuts at:      0    1925    3666 
   Size:       1925    1741
 
AleI CACnn'nnGTG

Cuts at:      0     592    3400    3666 
   Size:        592    2808     266

  Fragments arranged by size: 

                2808     592     266
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     610    3666 
   Size:        610    3056
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1013    1045    3666 
   Size:       1013      32    2621

  Fragments arranged by size: 

                2621    1013      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     423     766    1229    1516    1572    2112    2130    2847 
   Size:        423     343     463     287      56     540      18     717

Cuts at:   2847    3285    3604    3666 
   Size:        438     319      62

  Fragments arranged by size: 

                 717     540     463     438     423     343     319     287
                  62      56      18
 
ApaI G_GGCC'C

Cuts at:      0      35    3666 
   Size:         35    3631
 
ApoI r'AATT_y

Cuts at:      0    1807    3666 
   Size:       1807    1859
 
AseI AT'TA_AT

Cuts at:      0    3161    3666 
   Size:       3161     505
 
AvaI C'yCGr_G

Cuts at:      0    2425    2670    3666 
   Size:       2425     245     996

  Fragments arranged by size: 

                2425     996     245
 
AvrII C'CTAG_G

Cuts at:      0     285    3666 
   Size:        285    3381
 
BamHI G'GATC_C

Cuts at:      0    3475    3666 
   Size:       3475     191
 
BanI G'GyrC_C

Cuts at:      0     912    2506    2867    3666 
   Size:        912    1594     361     799

  Fragments arranged by size: 

                1594     912     799     361
 
BanII G_rGCy'C

Cuts at:      0      35     320     686     965    2761    2921    3666 
   Size:         35     285     366     279    1796     160     745

  Fragments arranged by size: 

                1796     745     366     285     279     160      35
 
BbeI G_GCGC'C

Cuts at:      0     916    3666 
   Size:        916    2750
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     366    1952    2048    2894    2984    3658    3666 
   Size:        366    1586      96     846      90     674       8

  Fragments arranged by size: 

                1586     846     674     366      96      90       8
 
BbvCI CC'TCA_GC

Cuts at:      0     406    1193    2651    3666 
   Size:        406     787    1458    1015

  Fragments arranged by size: 

                1458    1015     787     406
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3432    3466    3666 
   Size:       3432      34     200

  Fragments arranged by size: 

                3432     200      34
 
BclI T'GATC_A

Cuts at:      0    2480    3188    3666 
   Size:       2480     708     478

  Fragments arranged by size: 

                2480     708     478
 
BfrBI ATG'CAT

Cuts at:      0    1680    3666 
   Size:       1680    1986
 
BglI GCCn_nnn'nGGC

Cuts at:      0     622    3666 
   Size:        622    3044
 
BlpI GC'TnA_GC

Cuts at:      0      67    3666 
   Size:         67    3599
 
Bme1580I G_kGCm'C

Cuts at:      0      35     216    3666 
   Size:         35     181    3450

  Fragments arranged by size: 

                3450     181      35
 
BmgBI CAC'GTC

Cuts at:      0     138     975    1737    1958    3666 
   Size:        138     837     762     221    1708

  Fragments arranged by size: 

                1708     837     762     221     138
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     389     470    2274    3357    3666 
   Size:        389      81    1804    1083     309

  Fragments arranged by size: 

                1804    1083     389     309      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2829    3666 
   Size:       2829     837
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     554     801    1585    1732    1747    2297    2560    3666 
   Size:        554     247     784     147      15     550     263    1106

  Fragments arranged by size: 

                1106     784     554     550     263     247     147      15
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     406    1193    1573    2651    2755    3666 
   Size:        406     787     380    1078     104     911

  Fragments arranged by size: 

                1078     911     787     406     380     104
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1120    1289    3037    3368    3666 
   Size:       1120     169    1748     331     298

  Fragments arranged by size: 

                1748    1120     331     298     169
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     819    3666 
   Size:        819    2847
 
BsaAI yAC'GTr

Cuts at:      0    3016    3666 
   Size:       3016     650
 
BsaBI GATnn'nnATC

Cuts at:      0    1243    2380    2449    2857    3666 
   Size:       1243    1137      69     408     809

  Fragments arranged by size: 

                1243    1137     809     408      69
 
BsaHI Gr'CG_yC

Cuts at:      0     913    3666 
   Size:        913    2753
 
BsaWI w'CCGG_w

Cuts at:      0    1925    2798    3666 
   Size:       1925     873     868

  Fragments arranged by size: 

                1925     873     868
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     428     458     799     829    1013    1043    2785    2815 
   Size:        428      30     341      30     184      30    1742      30

Cuts at:   2815    3666 
   Size:        851

  Fragments arranged by size: 

                1742     851     428     341     184      30      30      30
                  30
 
BseYI C'CCAG_C

Cuts at:      0      27    3666 
   Size:         27    3639
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     330    1411    2125    2233    2512    2851    3666 
   Size:        330    1081     714     108     279     339     815

  Fragments arranged by size: 

                1081     815     714     339     330     279     108
 
BsiEI CG_ry'CG

Cuts at:      0     311    3666 
   Size:        311    3355
 
BsiHKAI G_wGCw'C

Cuts at:      0     320    1148    1652    3666 
   Size:        320     828     504    2014

  Fragments arranged by size: 

                2014     828     504     320
 
BsmI GAATG_Cn'

Cuts at:      0    2735    3666 
   Size:       2735     931
 
Bsp1286I G_dGCh'C

Cuts at:      0      35     216     320     686     965    1148    1652    2761 
   Size:         35     181     104     366     279     183     504    1109

Cuts at:   2761    2921    3666 
   Size:        160     745

  Fragments arranged by size: 

                1109     745     504     366     279     183     181     160
                 104      35
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1383    3666 
   Size:       1383    2283
 
BsrBI CCG'CTC

Cuts at:      0      71    3081    3666 
   Size:         71    3010     585

  Fragments arranged by size: 

                3010     585      71
 
BsrDI GCAATG_nn'

Cuts at:      0     756    2181    3482    3666 
   Size:        756    1425    1301     184

  Fragments arranged by size: 

                1425    1301     756     184
 
BsrFI r'CCGG_y

Cuts at:      0      81    1925    3517    3566    3666 
   Size:         81    1844    1592      49     100

  Fragments arranged by size: 

                1844    1592     100      81      49
 
BsrGI T'GTAC_A

Cuts at:      0     173    1494    1898    3666 
   Size:        173    1321     404    1768

  Fragments arranged by size: 

                1768    1321     404     173
 
BssHII G'CGCG_C

Cuts at:      0    3584    3666 
   Size:       3584      82
 
BssSI C'ACGA_G

Cuts at:      0    3441    3666 
   Size:       3441     225
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2112    3666 
   Size:       2112    1554
 
BstEII G'GTnAC_C

Cuts at:      0      42    1164    1326    2550    3666 
   Size:         42    1122     162    1224    1116

  Fragments arranged by size: 

                1224    1122    1116     162      42
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     328    1803    2005    3666 
   Size:        328    1475     202    1661

  Fragments arranged by size: 

                1661    1475     328     202
 
BstYI r'GATC_y

Cuts at:      0     476    2381    2417    3358    3475    3666 
   Size:        476    1905      36     941     117     191

  Fragments arranged by size: 

                1905     941     476     191     117      36
 
Bsu36I CC'TnA_GG

Cuts at:      0    1406    3666 
   Size:       1406    2260
 
BtgI C'CryG_G

Cuts at:      0      35      50     311    1331    2385    2936    3666 
   Size:         35      15     261    1020    1054     551     730

  Fragments arranged by size: 

                1054    1020     730     551     261      35      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3450    3666 
   Size:       3450     216
 
BtsI GCAGTG_nn'

Cuts at:      0    2591    3304    3407    3666 
   Size:       2591     713     103     259

  Fragments arranged by size: 

                2591     713     259     103
 
ClaI AT'CG_AT

Cuts at:      0    3128    3666 
   Size:       3128     538
 
DraI TTT'AAA

Cuts at:      0     522    1365    3666 
   Size:        522     843    2301

  Fragments arranged by size: 

                2301     843     522
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1566    2052    3666 
   Size:       1566     486    1614

  Fragments arranged by size: 

                1614    1566     486
 
EaeI y'GGCC_r

Cuts at:      0     623    2763    3276    3515    3591    3666 
   Size:        623    2140     513     239      76      75

  Fragments arranged by size: 

                2140     623     513     239      76      75
 
EarI CTCTTCn'nnn_

Cuts at:      0    2990    3666 
   Size:       2990     676
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     518    1084    3666 
   Size:        518     566    2582

  Fragments arranged by size: 

                2582     566     518
 
EcoICRI GAG'CTC

Cuts at:      0     318    3666 
   Size:        318    3348
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     289     554     801    1585    1732    1747    2116    2297 
   Size:        289     265     247     784     147      15     369     181

Cuts at:   2297    2560    2869    3666 
   Size:        263     309     797

  Fragments arranged by size: 

                 797     784     369     309     289     265     263     247
                 181     147      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     284    3666 
   Size:        284    3382
 
EcoO109I rG'GnC_Cy

Cuts at:      0      31     402     427    2089    2239    3666 
   Size:         31     371      25    1662     150    1427

  Fragments arranged by size: 

                1662    1427     371     150      31      25
 
EcoRV GAT'ATC

Cuts at:      0    1241    3666 
   Size:       1241    2425
 
HaeII r_GCGC'y

Cuts at:      0     916    3088    3666 
   Size:        916    2172     578

  Fragments arranged by size: 

                2172     916     578
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     264     296     974    1006    1329    1361    1437    1469 
   Size:        264      32     678      32     323      32      76      32

Cuts at:   1469    1723    1755    2270    2302    3420    3452    3666 
   Size:        254      32     515      32    1118      32     214

  Fragments arranged by size: 

                1118     678     515     323     264     254     214      76
                  32      32      32      32      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1620    1869    3560    3666 
   Size:       1620     249    1691     106

  Fragments arranged by size: 

                1691    1620     249     106
 
HindIII A'AGCT_T

Cuts at:      0    2634    3226    3666 
   Size:       2634     592     440

  Fragments arranged by size: 

                2634     592     440
 
KasI G'GCGC_C

Cuts at:      0     912    3666 
   Size:        912    2754
 
KpnI G_GTAC'C

Cuts at:      0    2871    3666 
   Size:       2871     795
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     586    1216    2411    2735    3666 
   Size:        113     473     630    1195     324     931

  Fragments arranged by size: 

                1195     931     630     473     324     113
 
MscI TGG'CCA

Cuts at:      0    2765    3278    3666 
   Size:       2765     513     388

  Fragments arranged by size: 

                2765     513     388
 
MslI CAynn'nnrTG

Cuts at:      0     106     326     592    3400    3666 
   Size:        106     220     266    2808     266

  Fragments arranged by size: 

                2808     266     266     220     106
 
MspA1I CmG'CkG

Cuts at:      0      37      52    1197    2130    2478    3666 
   Size:         37      15    1145     933     348    1188

  Fragments arranged by size: 

                1188    1145     933     348      37      15
 
NaeI GCC'GGC

Cuts at:      0      83    3568    3666 
   Size:         83    3485      98

  Fragments arranged by size: 

                3485      98      83
 
NarI GG'CG_CC

Cuts at:      0     913    3666 
   Size:        913    2753
 
NcoI C'CATG_G

Cuts at:      0    2936    3666 
   Size:       2936     730
 
NgoMIV G'CCGG_C

Cuts at:      0      81    3566    3666 
   Size:         81    3485     100

  Fragments arranged by size: 

                3485     100      81
 
NsiI A_TGCA'T

Cuts at:      0    1682    3666 
   Size:       1682    1984
 
NspI r_CATG'y

Cuts at:      0    1773    3666 
   Size:       1773    1893
 
PasI CC'CwG_GG

Cuts at:      0     423    2365    3666 
   Size:        423    1942    1301

  Fragments arranged by size: 

                1942    1301     423
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     766     978    3666 
   Size:        766     212    2688

  Fragments arranged by size: 

                2688     766     212
 
PfoI T'CCnGG_A

Cuts at:      0    1256    3666 
   Size:       1256    2410
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     952     984    1013    1045    1924    1956    2989    3021 
   Size:        952      32      29      32     879      32    1033      32

Cuts at:   3021    3666 
   Size:        645

  Fragments arranged by size: 

                1033     952     879     645      32      32      32      32
                  29
 
PpuMI rG'GwC_Cy

Cuts at:      0     402     427    3666 
   Size:        402      25    3239

  Fragments arranged by size: 

                3239     402      25
 
PshAI GACnn'nnGTC

Cuts at:      0     788    3666 
   Size:        788    2878
 
PsiI TTA'TAA

Cuts at:      0    1850    3666 
   Size:       1850    1816
 
PspOMI G'GGCC_C

Cuts at:      0      31    3666 
   Size:         31    3635
 
PstI C_TGCA'G

Cuts at:      0    1746    2586    2965    3557    3666 
   Size:       1746     840     379     592     109

  Fragments arranged by size: 

                1746     840     592     379     109
 
PvuII CAG'CTG

Cuts at:      0    1197    2130    3666 
   Size:       1197     933    1536

  Fragments arranged by size: 

                1536    1197     933
 
SacI G_AGCT'C

Cuts at:      0     320    3666 
   Size:        320    3346
 
SacII CC_GC'GG

Cuts at:      0      38      53    3666 
   Size:         38      15    3613

  Fragments arranged by size: 

                3613      38      15
 
SfcI C'TryA_G

Cuts at:      0    1564    1742    2511    2582    2961    3553    3571    3666 
   Size:       1564     178     769      71     379     592      18      95

  Fragments arranged by size: 

                1564     769     592     379     178      95      71      18
            
 
SfoI GGC'GCC

Cuts at:      0     914    3666 
   Size:        914    2752
 
SmaI CCC'GGG

Cuts at:      0    2672    3666 
   Size:       2672     994
 
SmlI C'TyrA_G

Cuts at:      0    1099    1304    3052    3383    3666 
   Size:       1099     205    1748     331     283

  Fragments arranged by size: 

                1748    1099     331     283     205
 
StuI AGG'CCT

Cuts at:      0    2399    2614    3666 
   Size:       2399     215    1052

  Fragments arranged by size: 

                2399    1052     215
 
StyI C'CwwG_G

Cuts at:      0     285    2004    2078    2936    3273    3666 
   Size:        285    1719      74     858     337     393

  Fragments arranged by size: 

                1719     858     393     337     285      74
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     481    1480    3666 
   Size:        481     999    2186

  Fragments arranged by size: 

                2186     999     481
 
TatI w'GTAC_w

Cuts at:      0     173    1494    1898    2543    3666 
   Size:        173    1321     404     645    1123

  Fragments arranged by size: 

                1321    1123     645     404     173
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     428     460     858     890    3666 
   Size:        428      32     398      32    2776

  Fragments arranged by size: 

                2776     428     398      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1264    3666 
   Size:       1264    2402
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2002    2943    3666 
   Size:       2002     941     723

  Fragments arranged by size: 

                2002     941     723
 
XmaI C'CCGG_G

Cuts at:      0    2670    3666 
   Size:       2670     996
 
XmnI GAAnn'nnTTC

Cuts at:      0    2794    3206    3666 
   Size:       2794     412     460

  Fragments arranged by size: 

                2794     460     412
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI   AflIII     AgeI     AleI     AlfI     AloI
    AlwNI     ApaI     ApoI     AseI     AvaI    AvrII    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI     PasI    PflMI
     PfoI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI    PvuII
     SacI    SacII     SfcI     SfoI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI     AfeI    AflII     AhdI    ApaLI     AscI
    AsiSI     BaeI    BciVI    BglII     BmtI    BseRI    BsiWI    BsmBI
    BspEI    BspHI    BstBI  BstZ17I    CspCI   DraIII     EagI    EcoRI
     FalI     FseI     FspI    FspAI     HpaI     MfeI     MluI     NdeI
     NheI     NotI     NruI     PacI     PciI     PmeI     PmlI    PspXI
     PsrI     PvuI    RsrII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     XbaI     XhoI     ZraI
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