Restriction map for pF1KB0047
##################################
# NO CUT ENZYMES (   60 enzymes) #
##################################

AatII       Acc65I      AccIII      AflII       AgeI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspI        AvrII       BbrPI       
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaWI       BseAI       BsiWI       BspEI       BspLU11I    
BssHII      Bst1107I    BstBI       BstZ17I     CelII       
CpoI        DraIII      Eco47III    FseI        HpaI        
KpnI        KspI        MroI        NdeI        NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
PvuI        RsrII       SacII       ScaI        SexAI       
SfuI        SgrAI       SpeI        SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         5678  GTCGAC               GT^[AC][GT]AC
AclI         4669  AACGTT               AA^CGTT
AflIII       5773  ACGCGT               A^C[GA][CT]GT
AocI          631  CCTCAGG              CC^T[ACGT]AGG
AviII        1161  TGCGCA               TGC^GCA
BanI         5921  GGCGCC               G^G[CT][GA]CC
BbeI         5921  GGCGCC               GGCGC^C
BbvCI        5874  GCTGAGG              CCTCAGC(-5/-2)
BcgI         3284  CGACCGACCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        4047  GGATAC               GTATCC(6/5)
BsaAI        3596  TACGTA               [CT]AC^GT[GA]
BsaBI        2214  GATCCGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI         5929  CTGCAC               GTGCAG(16/14)
BsmI         1642  GAATGC               GAATGC(1/-1)
BspDI        1555  ATCGAT               AT^CGAT
BstEII       2488  GGTTACC              G^GT[ACGT]ACC
BstPI        2488  GGTTACC              G^GT[ACGT]ACC
Bsu36I        631  CCTCAGG              CC^T[ACGT]AGG
ClaI         1555  ATCGAT               AT^CGAT
DraI         2442  TTTAAA               TTT^AAA
EagI         1239  CGGCCG               C^GGCCG
EclXI        1239  CGGCCG               C^GGCCG
Eco52I       1239  CGGCCG               C^GGCCG
Eco81I        631  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      2488  GGTTACC              G^GT[ACGT]ACC
EcoRI        2260  GAATTC               G^AATTC
EcoRV        2455  GATATC               GAT^ATC
FspI         1161  TGCGCA               TGC^GCA
KasI         5921  GGCGCC               G^GCGCC
MamI         2214  GATCCGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         5773  ACGCGT               A^CGCGT
NaeI         5918  GCCGGC               GCC^GGC
NarI         5921  GGCGCC               GG^CGCC
NgoMIV       5918  GCCGGC               G^CCGGC
NheI         5409  GCTAGC               G^CTAGC
Psp1406I     4669  AACGTT               AA^CGTT
SalI         5678  GTCGAC               G^TCGAC
SbfI         3291  CCTGCAGG             CCTGCA^GG
SfiI         2496  GGCCAGAAAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         5921  GGCGCC               GGC^GCC
SmaI         4559  CCCGGG               CCC^GGG
SnaBI        3596  TACGTA               TAC^GTA
SphI          491  GCATGC               GCATG^C
Sse8387I     3291  CCTGCAGG             CCTGCA^GG
XbaI         3756  TCTAGA               T^CTAGA
XmaCI        4559  CCCGGG               C^CCGGG
XmaI         4559  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcyI         5288  GGCGTC               G[GA]^CG[CT]C
             5921  GGCGCC              
AhdI         1368  GACCAAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2869  GACTTGGAGTC         
ApaI         4562  GGGCCC               GGGCC^C
             4697  GGGCCC              
AspEI        1368  GACCAAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2869  GACTTGGAGTC         
BclI         1131  TGATCA               T^GATCA
             4874  TGATCA              
BsaHI        5288  GGCGTC               G[GA]^CG[CT]C
             5921  GGCGCC              
BsmBI        3280  GAGACG               CGTCTC(1/5)
             5998  GAGACG              
Bsp1407I      422  TGTACA               T^GTACA
             2372  TGTACA              
BsrFI        5870  ACCGGC               [GA]^CCGG[CT]
             5918  GCCGGC              
BsrGI         422  TGTACA               T^GTACA
             2372  TGTACA              
Cfr10I       5870  ACCGGC               [GA]^CCGG[CT]
             5918  GCCGGC              
DrdI           49  GACAGGGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             3865  GACGCGCAGGTC        
Eam1105I     1368  GACCAAACGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2869  GACTTGGAGTC         
EcoNI        3819  CCTGCAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4365  CCTGCAGCAGG         
EcoT22I       489  ATGCAT               ATGCA^T
             1581  ATGCAT              
FbaI         1131  TGATCA               T^GATCA
             4874  TGATCA              
Hin1I        5288  GGCGTC               G[GA]^CG[CT]C
             5921  GGCGCC              
MfeI         4250  CAATTG               C^AATTG
             5164  CAATTG              
MunI         4250  CAATTG               C^AATTG
             5164  CAATTG              
NcoI          126  CCATGG               C^CATGG
             1016  CCATGG              
NsiI          489  ATGCAT               ATGCA^T
             1581  ATGCAT              
PflMI        4308  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5454  CCAGCAACTGG         
SspBI         422  TGTACA               T^GTACA
             2372  TGTACA              
StuI         2327  AGGCCT               AGG^CCT
             2504  AGGCCT              
Van91I       4308  CCAGCGCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             5454  CCAGCAACTGG         


#########################################
# 3 OR MORE CUT ENZYMES (  126 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        Asp700I     AspHI       
AvaI        AvaII       BalI        BamHI       BanII       
BbsI        BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaI        BsaJI       
BseRI       BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspHI       
BspMI       BsrBI       BsrDI       BsrI        BssKI       
BssSI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HincII      HindII      
HindIII     HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PaeR7I      PleI        PpuMI       
PstI        PvuII       RcaI        RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XcmI        XhoI        XhoII       
XmnI        
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