Restriction map for pF1KA2011
##################################
# NO CUT ENZYMES (   81 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AscI        AseI        AsnI        AspI        BbrPI       
BbvCI       BcgI        BclI        BfrI        BglII       
BsaAI       BsaBI       BseAI       BsiEI       BsiWI       
BspEI       BspHI       BspLU11I    BsrFI       BssHII      
Bst1107I    BstEII      BstPI       BstZ17I     Cfr10I      
CpoI        DraI        EagI        EclXI       Eco52I      
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
HincII      HindII      HindIII     HpaI        KspI        
MamI        MfeI        MluI        MroI        MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        SgrAI       SnaBI       SpeI        
SphI        Sse8387I    SspI        SwaI        Tth111I     
XbaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

Acc65I       1405  GGTACC               G^GTACC
AccI         2269  GTCTAC               GT^[AC][GT]AC
AcsI          721  GAATTC               [GA]^AATT[CT]
AcyI         1945  GGCGCC               G[GA]^CG[CT]C
AflIII        183  ACGTGT               A^C[GA][CT]GT
AhdI         1633  GACACCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2299  GTGCAC               G^TGCAC
AocI          876  CCTGAGG              CC^T[ACGT]AGG
ApaLI        2299  GTGCAC               G^TGCAC
ApoI          721  GAATTC               [GA]^AATT[CT]
Asp700I      2349  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1405  GGTACC               G^GTACC
AspEI        1633  GACACCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        2074  CCTAGG               C^CTAGG
BamHI        2363  GGATCC               G^GATCC
BbeI         1945  GGCGCC               GGCGC^C
BciVI         657  GGATAC               GTATCC(6/5)
BlnI         2074  CCTAGG               C^CTAGG
BsaHI        1945  GGCGCC               G[GA]^CG[CT]C
BsaI         1861  GAGACC               GGTCTC(1/5)
BsgI         1346  CTGCAC               GTGCAG(16/14)
BsmI         2442  GAATGC               GAATGC(1/-1)
BspDI         901  ATCGAT               AT^CGAT
BsrDI        1043  GCAATG               GCAATG(2/0)
BstAPI       2015  GCATGACCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1216  TTCGAA               TT^CGAA
Bsu36I        876  CCTGAGG              CC^T[ACGT]AGG
ClaI          901  ATCGAT               AT^CGAT
DraIII        224  CACCCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2527  GACTGGCGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1633  GACACCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        876  CCTGAGG              CC^T[ACGT]AGG
EcoRI         721  GAATTC               G^AATTC
Hin1I        1945  GGCGCC               G[GA]^CG[CT]C
KasI         1945  GGCGCC               G^GCGCC
KpnI         1405  GGTACC               GGTAC^C
MseI         1852  TTAA                 T^TAA
NarI         1945  GGCGCC               GG^CGCC
NcoI         2738  CCATGG               C^CATGG
NspI          113  GCATGT               [GA]CATG^[CT]
NspV         1216  TTCGAA               TT^CGAA
PaeR7I        164  CTCGAG               C^TCGAG
ScaI          845  AGTACT               AGT^ACT
SexAI         661  ACCTGGT              A^CC[AT]GGT
SfoI         1945  GGCGCC               GGC^GCC
SfuI         1216  TTCGAA               TT^CGAA
Tru9I        1852  TTAA                 T^TAA
Tsp509I       722  AATT                 ^AATT
XhoI          164  CTCGAG               C^TCGAG
XmnI         2349  GAAGAGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccII         889  CGCG                 CG^CG
             1879  CGCG                
Aor51HI      2677  AGCGCT               AGC^GCT
             2868  AGCGCT              
AviII        1671  TGCGCA               TGC^GCA
             2516  TGCGCA              
BsaWI         437  ACCGGA               [AT]^CCGG[AT]
             1395  ACCGGA              
BsmBI        2151  GAGACG               CGTCTC(1/5)
             2428  GAGACG              
BsrBI         919  GAGCGG               CCGCTC(-3/-3)
             1609  GAGCGG              
BssSI        2719  CACGAG               CACGAG(-5/-1)
             2474  CTCGTG              
BstUI         889  CGCG                 CG^CG
             1879  CGCG                
BstXI          64  CCACGCCGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              578  CCAATGGGCTGG        
Eco47III     2677  AGCGCT               AGC^GCT
             2868  AGCGCT              
FspI         1671  TGCGCA               TGC^GCA
             2516  TGCGCA              
HgaI         2153  GACGC                GACGC(5/10)
             3021  GCGTC               
MaeII         183  ACGT                 A^CGT
             2504  ACGT                
MslI           53  CACCACGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1800  CACGGGCATG          
MvnI          889  CGCG                 CG^CG
             1879  CGCG                
PpuMI         159  AGGTCCT              [GA]G^G[AT]CC[CT]
              624  AGGACCT             
SacI          291  GAGCTC               GAGCT^C
             2434  GAGCTC              
SapI         2349  GAAGAGC              GCTCTTC(1/4)
             2806  GAAGAGC             
SfiI          930  GGCCGACTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1719  GGCCGATGTGGCC       
XcmI         1466  CCAGAGCCAAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2228  CCAGCCCTTTCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  107 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
ApaI        AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    Bsp1407I    BspMI       BsrGI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CelII       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        SmlI        SspBI       
StuI        StyI        TaqI        TfiI        TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XhoII       
XmaCI       XmaI        
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