Restriction map for pF1KA1997
 (Circular) (Six-base) MAPSORT of: pF1KA1997.seq  Check: 9867  from: 1  to: 16034



pF1KA1997  16034 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1296    1296 
   Size:      16034
 
AccI GT'mk_AC

Cuts at:   4438    5059    7142   12726   13072    4438 
   Size:        621    2083    5584     346    7400

  Fragments arranged by size: 

                7400    5584    2083     621     346
 
Acc65I G'GTAC_C

Cuts at:  13050   13050 
   Size:      16034
 
AclI AA'CG_TT

Cuts at:  11241   15874   11241 
   Size:       4633   11401
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    377     493    3098    3223    4489    4772    4991    7489    8188 
   Size:        116    2605     125    1266     283     219    2498     699

Cuts at:   8188    8285    8995    9487    9497    9548    9608   11605   11685 
   Size:         97     710     492      10      51      60    1997      80

Cuts at:  11685   11911   11942   11959   13859   14291   15038   15306     377 
   Size:        226      31      17    1900     432     747     268    1105

  Fragments arranged by size: 

                2605    2498    1997    1900    1266    1105     747     710
                 699     492     432     283     268     226     219     125
                 116      97      80      60      51      31      17      10
            
 
AfeI AGC'GCT

Cuts at:   1030    2257    1030 
   Size:       1227   14807
 
AflII C'TTAA_G

Cuts at:   6835    8359    6835 
   Size:       1524   14510
 
AflIII A'CryG_T

Cuts at:   5023    8193    8736   10649   10904   13386   14490   15467    5023 
   Size:       3170     543    1913     255    2482    1104     977    5590

  Fragments arranged by size: 

                5590    3170    2482    1913    1104     977     543     255
            
 
AgeI A'CCGG_T

Cuts at:  14422   14422 
   Size:      16034
 
AhdI GACnn_n'nnGTC

Cuts at:   7809    7809 
   Size:      16034
 
AleI CACnn'nnGTG

Cuts at:    204     204 
   Size:      16034
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    268    7018   12928     268 
   Size:       6750    5910    3374

  Fragments arranged by size: 

                6750    5910    3374
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    878     910   10721   10753     878 
   Size:         32    9811      32    6159

  Fragments arranged by size: 

                9811    6159      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:    201    7098    8908    9571   13339   14906     201 
   Size:       6897    1810     663    3768    1567    1329

  Fragments arranged by size: 

                6897    3768    1810    1567    1329     663
 
ApaLI G'TGCA_C

Cuts at:   7064   10511   14804    7064 
   Size:       3447    4293    8294

  Fragments arranged by size: 

                8294    4293    3447
 
ApoI r'AATT_y

Cuts at:    586    1334    1502    1933    2061    2418    2760    3157    3265 
   Size:        748     168     431     128     357     342     397     108

Cuts at:   3265    3494    3589    4128    4302    4357    4744    4952    5050 
   Size:        229      95     539     174      55     387     208      98

Cuts at:   5050    5332    5451    5465    6306    6667    6727    7278    7502 
   Size:        282     119      14     841     361      60     551     224

Cuts at:   7502    7555    7977    8420    8463    8640    9113    9422    9547 
   Size:         53     422     443      43     177     473     309     125

Cuts at:   9547    9817   10201   10553   11622   11776   11854   12065   12196 
   Size:        270     384     352    1069     154      78     211     131

Cuts at:  12196   12264   12703   13038   15387   15643     586 
   Size:         68     439     335    2349     256     977

  Fragments arranged by size: 

                2349    1069     977     841     748     551     539     473
                 443     439     431     422     397     387     384     361
                 357     352     342     335     309     282     270     256
                 229     224     211     208     177     174     168     154
                 131     128     125     119     108      98      95      78
                  68      60      55      53      43      14
 
AseI AT'TA_AT

Cuts at:     20    2837    3940    6677    8677    9635   10643      20 
   Size:       2817    1103    2737    2000     958    1008    5411

  Fragments arranged by size: 

                5411    2817    2737    2000    1103    1008     958
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:      16034
 
AvaI C'yCGr_G

Cuts at:   1184   13054    1184 
   Size:      11870    4164
 
AvrII C'CTAG_G

Cuts at:   3766    3766 
   Size:      16034
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2936    2969    3812    3845    7519    7552    2936 
   Size:         33     843      33    3674      33   11418

  Fragments arranged by size: 

               11418    3674     843      33      33      33
 
BamHI G'GATC_C

Cuts at:   4854    7648   13059    4854 
   Size:       2794    5411    7829

  Fragments arranged by size: 

                7829    5411    2794
 
BanI G'GyrC_C

Cuts at:   1493   11833   13050   13464   13712   13747    1493 
   Size:      10340    1217     414     248      35    3780

  Fragments arranged by size: 

               10340    3780    1217     414     248      35
 
BanII G_rGCy'C

Cuts at:   1244    7792    7842    8345    9720   10698   11691   13048   14078 
   Size:       6548      50     503    1375     978     993    1357    1030

Cuts at:  14078    1244 
   Size:       3200

  Fragments arranged by size: 

                6548    3200    1375    1357    1030     993     978     503
                  50
 
BbeI G_GCGC'C

Cuts at:  13468   13716   13468 
   Size:        248   15786
 
BbsI GAAGACnn'nnnn_

Cuts at:   2860    3086    3229    3554    3959    4777    4979    5235    5514 
   Size:        226     143     325     405     818     202     256     279

Cuts at:   5514    5722    6854    7344    7353    8291    8391    9248    9485 
   Size:        208    1132     490       9     938     100     857     237

Cuts at:   9485    2860 
   Size:       9409

  Fragments arranged by size: 

                9409    1132     938     857     818     490     405     325
                 279     256     237     226     208     202     143     100
                   9
 
BbvCI CC'TCA_GC

Cuts at:   5681    5681 
   Size:      16034
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   3469    3503   10816   10850    3469 
   Size:         34    7313      34    8653

  Fragments arranged by size: 

                8653    7313      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:    622    6783   10869   13932   14693   16034     622 
   Size:       6161    4086    3063     761    1341     622

  Fragments arranged by size: 

                6161    4086    3063    1341     761     622
 
BclI T'GATC_A

Cuts at:    828    1989    4323    5016    8282    8921   10563   10806   10941 
   Size:       1161    2334     693    3266     639    1642     243     135

Cuts at:  10941   12134   13554     828 
   Size:       1193    1420    3308

  Fragments arranged by size: 

                3308    3266    2334    1642    1420    1193    1161     693
                 639     243     135
 
BfrBI ATG'CAT

Cuts at:   1516    2334    2338    8121   10845   12877    1516 
   Size:        818       4    5783    2724    2032    4673

  Fragments arranged by size: 

                5783    4673    2724    2032     818       4
 
BglII A'GATC_T

Cuts at:   1671    1756    2640    3242    6468   10134   11790   13549   15586 
   Size:         85     884     602    3226    3666    1656    1759    2037

Cuts at:  15586    1671 
   Size:       2119

  Fragments arranged by size: 

                3666    3226    2119    2037    1759    1656     884     602
                  85
 
BlpI GC'TnA_GC

Cuts at:   2292    4377    9344   13146    2292 
   Size:       2085    4967    3802    5180

  Fragments arranged by size: 

                5180    4967    3802    2085
 
Bme1580I G_kGCm'C

Cuts at:   3492    3870    6756    7068    9940   10515   11421   12552   13659 
   Size:        378    2886     312    2872     575     906    1131    1107

Cuts at:  13659   13752   14808    3492 
   Size:         93    1056    4718

  Fragments arranged by size: 

                4718    2886    2872    1131    1107    1056     906     575
                 378     312      93
 
BmgBI CAC'GTC

Cuts at:   3235    3235 
   Size:      16034
 
BmrI ACTGGGnnnn_n'

Cuts at:   2982    4679    4986    5195    6338    7351   11003   12576   13246 
   Size:       1697     307     209    1143    1013    3652    1573     670

Cuts at:  13246   13327   13662   15806    2982 
   Size:         81     335    2144    3210

  Fragments arranged by size: 

                3652    3210    2144    1697    1573    1143    1013     670
                 335     307     209      81
 
BmtI G_CTAG'C

Cuts at:  10408   10408 
   Size:      16034
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:  11452   12924   14401   11452 
   Size:       1472    1477   13085

  Fragments arranged by size: 

               13085    1477    1472
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2398    3710    4903    6752    7352    8069    8882   11591    2398 
   Size:       1312    1193    1849     600     717     813    2709    6841

  Fragments arranged by size: 

                6841    2709    1849    1312    1193     813     717     600
            
 
Bpu10I CC'TnA_GC

Cuts at:   2037    5681    7009   11654    2037 
   Size:       3644    1328    4645    6417

  Fragments arranged by size: 

                6417    4645    3644    1328
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4667    5120    6227    7912    8263    9601    9832   10130   10433 
   Size:        453    1107    1685     351    1338     231     298     303

Cuts at:  10433   13210   14089   14581   14879    4667 
   Size:       2777     879     492     298    5822

  Fragments arranged by size: 

                5822    2777    1685    1338    1107     879     492     453
                 351     303     298     298     231
 
BsaI GGTCTCn'nnnn_

Cuts at:  15738   15738 
   Size:      16034
 
BsaAI yAC'GTr

Cuts at:   3901   10939   13387    3901 
   Size:       7038    2448    6548

  Fragments arranged by size: 

                7038    6548    2448
 
BsaBI GATnn'nnATC

Cuts at:      6     374     833    3014    3419    6323    9181    9456   10580 
   Size:        368     459    2181     405    2904    2858     275    1124

Cuts at:  10580   12794   13548   15585       6 
   Size:       2214     754    2037     455

  Fragments arranged by size: 

                2904    2858    2214    2181    2037    1124     754     459
                 455     405     368     275
 
BsaHI Gr'CG_yC

Cuts at:  13465   13713   14415   15911   13465 
   Size:        248     702    1496   13588

  Fragments arranged by size: 

               13588    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   7031   10248   11154   12894   13223   13744   14422   14696   14843 
   Size:       3217     906    1740     329     521     678     274     147

Cuts at:  14843   15254    7031 
   Size:        411    7811

  Fragments arranged by size: 

                7811    3217    1740     906     678     521     411     329
                 274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   2371    2401    4541    4571    8002    8032    8524    8554    2371 
   Size:         30    2140      30    3431      30     492      30    9851

  Fragments arranged by size: 

                9851    3431    2140     492      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    247    6755    9514    9843   12284     247 
   Size:       6508    2759     329    2441    3997

  Fragments arranged by size: 

                6508    3997    2759    2441     329
 
BseYI C'CCAG_C

Cuts at:    535   10976   11800   12826   13459   14217   14794   15290     535 
   Size:      10441     824    1026     633     758     577     496    1279

  Fragments arranged by size: 

               10441    1279    1026     824     758     633     577     496
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    286    1862    4709    9350     286 
   Size:       1576    2847    4641    6970

  Fragments arranged by size: 

                6970    4641    2847    1576
 
BsiEI CG_ry'CG

Cuts at:    104    4179   12306   13576   13622   14830     104 
   Size:       4075    8127    1270      46    1208    1308

  Fragments arranged by size: 

                8127    4075    1308    1270    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:   6222    7068    7842    9720   10515   10698   11691   12845   13048 
   Size:        846     774    1878     795     183     993    1154     203

Cuts at:  13048   13826   14016   14808    6222 
   Size:        778     190     792    7448

  Fragments arranged by size: 

                7448    1878    1154     993     846     795     792     778
                 774     203     190     183
 
BsmI GAATG_Cn'

Cuts at:   1366    2531    4381    5784    7234    7598    9345    9672    1366 
   Size:       1165    1850    1403    1450     364    1747     327    7728

  Fragments arranged by size: 

                7728    1850    1747    1450    1403    1165     364     327
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:   3757   10122    3757 
   Size:       6365    9669
 
Bsp1286I G_dGCh'C

Cuts at:   1244    3492    3870    6222    6756    7068    7792    7842    8345 
   Size:       2248     378    2352     534     312     724      50     503

Cuts at:   8345    9720    9940   10515   10698   11421   11691   12552   12845 
   Size:       1375     220     575     183     723     270     861     293

Cuts at:  12845   13048   13659   13752   13826   14016   14078   14808    1244 
   Size:        203     611      93      74     190      62     730    2470

  Fragments arranged by size: 

                2470    2352    2248    1375     861     730     724     723
                 611     575     534     503     378     312     293     270
                 220     203     190     183      93      74      62      50
            
 
BspEI T'CCGG_A

Cuts at:  12894   15254   12894 
   Size:       2360   13674
 
BspHI T'CATG_A

Cuts at:   1128    6396    6660    8474   15210    1128 
   Size:       5268     264    1814    6736    1952

  Fragments arranged by size: 

                6736    5268    1952    1814     264
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1161    1296    4130    5931   13084   13298   13600   13981    1161 
   Size:        135    2834    1801    7153     214     302     381    3214

  Fragments arranged by size: 

                7153    3214    2834    1801     381     302     214     135
            
 
BsrBI CCG'CTC

Cuts at:     50    8128   14326   14380   15864   16029      50 
   Size:       8078    6198      54    1484     165      55

  Fragments arranged by size: 

                8078    6198    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1922    4186    4538   11430   13946    1922 
   Size:       2264     352    6892    2516    4010

  Fragments arranged by size: 

                6892    4010    2516    2264     352
 
BsrFI r'CCGG_y

Cuts at:  14032   14213   14422   14032 
   Size:        181     209   15644

  Fragments arranged by size: 

               15644     209     181
 
BsrGI T'GTAC_A

Cuts at:   2366    2742    8259   11844   11859    2366 
   Size:        376    5517    3585      15    6541

  Fragments arranged by size: 

                6541    5517    3585     376      15
 
BssSI C'ACGA_G

Cuts at:    605    6755   14305   14663     605 
   Size:       6150    7550     358    1976

  Fragments arranged by size: 

                7550    6150    1976     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1924    8433    8704    8908   10510   13411    1924 
   Size:       6509     271     204    1602    2901    4547

  Fragments arranged by size: 

                6509    4547    2901    1602     271     204
 
BstBI TT'CG_AA

Cuts at:  14395   14395 
   Size:      16034
 
BstEII G'GTnAC_C

Cuts at:  12948   12948 
   Size:      16034
 
BstXI CCAn_nnnn'nTGG

Cuts at:   3734    5148    7227    3734 
   Size:       1414    2079   12541

  Fragments arranged by size: 

               12541    2079    1414
 
BstYI r'GATC_y

Cuts at:      1     930    1671    1756    2640    3242    4854    6468    7519 
   Size:        929     741      85     884     602    1612    1614    1051

Cuts at:   7519    7648    9951   10134   11790   12175   12313   12513   13059 
   Size:        129    2303     183    1656     385     138     200     546

Cuts at:  13059   13526   13549   13884   14130   15142   15228   15240   15586 
   Size:        467      23     335     246    1012      86      12     346

Cuts at:  15586       1 
   Size:        449

  Fragments arranged by size: 

                2303    1656    1614    1612    1051    1012     929     884
                 741     602     546     467     449     385     346     335
                 246     200     183     138     129      86      85      23
                  12
 
BstZ17I GTA'TAC

Cuts at:   5060    7143    5060 
   Size:       2083   13951
 
Bsu36I CC'TnA_GG

Cuts at:    259   11753     259 
   Size:      11494    4540
 
BtgI C'CryG_G

Cuts at:    107     660    1045    4179    6937   10982   11174   11369     107 
   Size:        553     385    3134    2758    4045     192     195    4772

  Fragments arranged by size: 

                4772    4045    3134    2758     553     385     195     192
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    641   13984   14165   14328     641 
   Size:      13343     181     163    2347

  Fragments arranged by size: 

               13343    2347     181     163
 
BtsI GCAGTG_nn'

Cuts at:   3935    8082   10515   11221   11534   12683   13277    3935 
   Size:       4147    2433     706     313    1149     594    6692

  Fragments arranged by size: 

                6692    4147    2433    1149     706     594     313
 
ClaI AT'CG_AT

Cuts at:   5264    5264 
   Size:      16034
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1433    1468    7026    7061   12659   12694   12970   13005    1433 
   Size:         35    5558      35    5598      35     276      35    4462

  Fragments arranged by size: 

                5598    5558    4462     276      35      35      35      35
            
 
DraI TTT'AAA

Cuts at:    702    2766    2831    3270    3857    4133    4566    5613    9312 
   Size:       2064      65     439     587     276     433    1047    3699

Cuts at:   9312   11615   13033     702 
   Size:       2303    1418    3703

  Fragments arranged by size: 

                3703    3699    2303    2064    1418    1047     587     439
                 433     276      65
 
DraIII CAC_nnn'GTG

Cuts at:   3874    3874 
   Size:      16034
 
DrdI GACnn_nn'nnGTC

Cuts at:   4267   13740   14598    4267 
   Size:       9473     858    5703

  Fragments arranged by size: 

                9473    5703     858
 
EaeI y'GGCC_r

Cuts at:   3318    3539    4176   13619   13793   14184   14211   15556    3318 
   Size:        221     637    9443     174     391      27    1345    3796

  Fragments arranged by size: 

                9443    3796    1345     637     391     221     174      27
            
 
EagI C'GGCC_G

Cuts at:   4176   13619    4176 
   Size:       9443    6591
 
EarI CTCTTCn'nnn_

Cuts at:    145    1097    1157    2512    3431    3506    5945    6455    6890 
   Size:        952      60    1355     919      75    2439     510     435

Cuts at:   6890    9710   10430   11507   11704   12599   12659   14057   14267 
   Size:       2820     720    1077     197     895      60    1398     210

Cuts at:  14267     145 
   Size:       1912

  Fragments arranged by size: 

                2820    2439    1912    1398    1355    1077     952     919
                 895     720     510     435     210     197      75      60
                  60
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   4747   10195   14242   14552   14698   15844    4747 
   Size:       5448    4047     310     146    1146    4937

  Fragments arranged by size: 

                5448    4937    4047    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   7840    9718   10696   11689   13046    7840 
   Size:       1878     978     993    1357   10828

  Fragments arranged by size: 

               10828    1878    1357     993     978
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    377     493    2398    3098    3223    3710    4489    4772    4903 
   Size:        116    1905     700     125     487     779     283     131

Cuts at:   4903    4991    6752    7352    7489    8069    8188    8285    8882 
   Size:         88    1761     600     137     580     119      97     597

Cuts at:   8882    8995    9487    9497    9548    9608   11591   11605   11685 
   Size:        113     492      10      51      60    1983      14      80

Cuts at:  11685   11911   11942   11959   13859   14291   15038   15306     377 
   Size:        226      31      17    1900     432     747     268    1105

  Fragments arranged by size: 

                1983    1905    1900    1761    1105     779     747     700
                 600     597     580     492     487     432     283     268
                 226     137     131     125     119     116     113      97
                  88      80      60      51      31      17      14      10
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1102    3583    7243    1102 
   Size:       2481    3660    9893

  Fragments arranged by size: 

                9893    3660    2481
 
EcoO109I rG'GnC_Cy

Cuts at:   2474    5312   13173    2474 
   Size:       2838    7861    5335

  Fragments arranged by size: 

                7861    5335    2838
 
EcoRI G'AATT_C

Cuts at:   1502    2061    6306    6667    8420    9422   10201   12264   13038 
   Size:        559    4245     361    1753    1002     779    2063     774

Cuts at:  13038    1502 
   Size:       4498

  Fragments arranged by size: 

                4498    4245    2063    1753    1002     779     774     559
                 361
 
EcoRV GAT'ATC

Cuts at:   3477    4251    6154    7188    3477 
   Size:        774    1903    1034   12323

  Fragments arranged by size: 

               12323    1903    1034     774
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1451    5139    5961    9322   10441   10546   10632   14053    1451 
   Size:       3688     822    3361    1119     105      86    3421    3432

  Fragments arranged by size: 

                3688    3432    3421    3361    1119     822     105      86
            
 
FspI TGC'GCA

Cuts at:  13815   13815 
   Size:      16034
 
HaeII r_GCGC'y

Cuts at:   1032    2259    7707   13468   13716   14738   15717    1032 
   Size:       1227    5448    5761     248    1022     979    1349

  Fragments arranged by size: 

                5761    5448    1349    1227    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    736     768    1705    1720    1737    1741    1752    1773    1992 
   Size:         32     937      15      17       4      11      21     219

Cuts at:   1992    2024    2853    2885    3162    3194    3234    3266    4016 
   Size:         32     829      32     277      32      40      32     750

Cuts at:   4016    4048    4066    4098    4219    4251    4446    4478    4498 
   Size:         32      18      32     121      32     195      32      20

Cuts at:   4498    4530    5257    5289    6238    6270    6285    6317    7774 
   Size:         32     727      32     949      32      15      32    1457

Cuts at:   7774    7795    7806    7827    8002    8034    8524    8556   10119 
   Size:         21      11      21     175      32     490      32    1563

Cuts at:  10119   10151   11077   11109   12126   12158   13876   13906   13908 
   Size:         32     926      32    1017      32    1718      30       2

Cuts at:  13908   13938     736 
   Size:         30    2832

  Fragments arranged by size: 

                2832    1718    1563    1457    1017     949     937     926
                 829     750     727     490     277     219     195     175
                 121      40      32      32      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      30      30      21      21
                  21      20      18      17      15      15      11      11
                   4       2
 
HincII GTy'rAC

Cuts at:    771    1557    3669    4695    6378   10220   10278   12727   13073 
   Size:        786    2112    1026    1683    3842      58    2449     346

Cuts at:  13073     771 
   Size:       3732

  Fragments arranged by size: 

                3842    3732    2449    2112    1683    1026     786     346
                  58
 
HindIII A'AGCT_T

Cuts at:   3893    4474    8369    3893 
   Size:        581    3895   11558

  Fragments arranged by size: 

               11558    3895     581
 
HpaI GTT'AAC

Cuts at:   6378   10278    6378 
   Size:       3900   12134
 
KasI G'GCGC_C

Cuts at:  13464   13712   13464 
   Size:        248   15786
 
KpnI G_GTAC'C

Cuts at:  13054   13054 
   Size:      16034
 
MfeI C'AATT_G

Cuts at:    719    3824     719 
   Size:       3105   12929
 
MluI A'CGCG_T

Cuts at:  15467   15467 
   Size:      16034
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    299     320    1423    1562    3903    3993    8071    9063   10704 
   Size:         21    1103     139    2341      90    4078     992    1641

Cuts at:  10704   10713   10878   11740   14705   14889     299 
   Size:          9     165     862    2965     184    1444

  Fragments arranged by size: 

                4078    2965    2341    1641    1444    1103     992     862
                 184     165     139      90      21       9
 
MscI TGG'CCA

Cuts at:   3320    3541   13795    3320 
   Size:        221   10254    5559

  Fragments arranged by size: 

               10254    5559     221
 
MslI CAynn'nnrTG

Cuts at:    204    1661    2933    5146    5266    6644    7061    8120   14023 
   Size:       1457    1272    2213     120    1378     417    1059    5903

Cuts at:  14023   14150     204 
   Size:        127    2088

  Fragments arranged by size: 

                5903    2213    2088    1457    1378    1272    1059     417
                 127     120
 
MspA1I CmG'CkG

Cuts at:   2662    5179    7330    9364    9805   12187   13145   13459   13819 
   Size:       2517    2151    2034     441    2382     958     314     360

Cuts at:  13819   14832   15077    2662 
   Size:       1013     245    3619

  Fragments arranged by size: 

                3619    2517    2382    2151    2034    1013     958     441
                 360     314     245
 
NaeI GCC'GGC

Cuts at:  14215   14215 
   Size:      16034
 
NarI GG'CG_CC

Cuts at:  13465   13713   13465 
   Size:        248   15786
 
NcoI C'CATG_G

Cuts at:    107    1045    6937   11174   11369     107 
   Size:        938    5892    4237     195    4772

  Fragments arranged by size: 

                5892    4772    4237     938     195
 
NdeI CA'TA_TG

Cuts at:   1174    6828    7692    8700   10766   11595    1174 
   Size:       5654     864    1008    2066     829    5613

  Fragments arranged by size: 

                5654    5613    2066    1008     864     829
 
NgoMIV G'CCGG_C

Cuts at:  14213   14213 
   Size:      16034
 
NheI G'CTAG_C

Cuts at:  10404   10404 
   Size:      16034
 
NsiI A_TGCA'T

Cuts at:   1518    2336    2340    8123   10847   12879    1518 
   Size:        818       4    5783    2724    2032    4673

  Fragments arranged by size: 

                5783    4673    2724    2032     818       4
 
NspI r_CATG'y

Cuts at:   2334    2338    5027    6912    8121    8197    8740    9132   10653 
   Size:          4    2689    1885    1209      76     543     392    1521

Cuts at:  10653   10845   10908   11192   11729   12881   13087   13587   14494 
   Size:        192      63     284     537    1152     206     500     907

Cuts at:  14494    2334 
   Size:       3874

  Fragments arranged by size: 

                3874    2689    1885    1521    1209    1152     907     543
                 537     500     392     284     206     192      76      63
                   4
 
PacI TTA_AT'TAA

Cuts at:   9635    9635 
   Size:      16034
 
PciI A'CATG_T

Cuts at:   5023    8193    8736   10649   10904   14490    5023 
   Size:       3170     543    1913     255    3586    6567

  Fragments arranged by size: 

                6567    3586    3170    1913     543     255
 
PflMI CCAn_nnn'nTGG

Cuts at:   1531    2994    6024    9414    1531 
   Size:       1463    3030    3390    8151

  Fragments arranged by size: 

                8151    3390    3030    1463
 
PfoI T'CCnGG_A

Cuts at:   4880    5886    8313    4880 
   Size:       1006    2427   12601

  Fragments arranged by size: 

               12601    2427    1006
 
PmeI GTTT'AAAC

Cuts at:  13033   13033 
   Size:      16034
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:  15173   15205   15173 
   Size:         32   16002
 
PsiI TTA'TAA

Cuts at:    737    1979    4061   11063     737 
   Size:       1242    2082    7002    5708

  Fragments arranged by size: 

                7002    5708    2082    1242
 
PspXI vC'TCGA_Gb

Cuts at:   1184    1184 
   Size:      16034
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   7439    7471   10094   10126    7439 
   Size:         32    2623      32   13347

  Fragments arranged by size: 

               13347    2623      32      32
 
PstI C_TGCA'G

Cuts at:   4127    7094    9363    9624   11541   12034   13081   13766    4127 
   Size:       2967    2269     261    1917     493    1047     685    6395

  Fragments arranged by size: 

                6395    2967    2269    1917    1047     685     493     261
            
 
PvuI CG_AT'CG

Cuts at:    104   12306     104 
   Size:      12202    3832
 
PvuII CAG'CTG

Cuts at:   2662    5179    7330    9364    9805   12187   13459   13819    2662 
   Size:       2517    2151    2034     441    2382    1272     360    4877

  Fragments arranged by size: 

                4877    2517    2382    2151    2034    1272     441     360
            
 
RsrII CG'GwC_CG

Cuts at:  14229   14229 
   Size:      16034
 
SacI G_AGCT'C

Cuts at:   7842    9720   10698   11691   13048    7842 
   Size:       1878     978     993    1357   10828

  Fragments arranged by size: 

               10828    1878    1357     993     978
 
SalI G'TCGA_C

Cuts at:  12725   13071   12725 
   Size:        346   15688
 
SapI GCTCTTCn'nnn_

Cuts at:    145    3431   12599   12659   14057   14267     145 
   Size:       3286    9168      60    1398     210    1912

  Fragments arranged by size: 

                9168    3286    1912    1398     210      60
 
SbfI CC_TGCA'GG

Cuts at:  13081   13081 
   Size:      16034
 
ScaI AGT'ACT

Cuts at:   7125    7224    9357   15551    7125 
   Size:         99    2133    6194    7608

  Fragments arranged by size: 

                7608    6194    2133      99
 
SexAI A'CCwGG_T

Cuts at:  11360   11360 
   Size:      16034
 
SfcI C'TryA_G

Cuts at:     33    2300    4123    7049    7090    7394    9359    9620    9968 
   Size:       2267    1823    2926      41     304    1965     261     348

Cuts at:   9968   11537   11572   12030   13077   13762   14755   14946      33 
   Size:       1569      35     458    1047     685     993     191    1121

  Fragments arranged by size: 

                2926    2267    1965    1823    1569    1121    1047     993
                 685     458     348     304     261     191      41      35
            
 
SfoI GGC'GCC

Cuts at:  13466   13714   13466 
   Size:        248   15786
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:      16034
 
SmaI CCC'GGG

Cuts at:  13056   13056 
   Size:      16034
 
SmlI C'TyrA_G

Cuts at:   1184    4682    5135    6242    6835    7927    8242    8359    9580 
   Size:       3498     453    1107     593    1092     315     117    1221

Cuts at:   9580    9811   10145   10448   13189   14104   14596   14858    1184 
   Size:        231     334     303    2741     915     492     262    2360

  Fragments arranged by size: 

                3498    2741    2360    1221    1107    1092     915     593
                 492     453     334     315     303     262     231     117
            
 
SpeI A'CTAG_T

Cuts at:     67    3775      67 
   Size:       3708   12326
 
SphI G_CATG'C

Cuts at:   2338    9132   10845   13087   13587    2338 
   Size:       6794    1713    2242     500    4785

  Fragments arranged by size: 

                6794    4785    2242    1713     500
 
SspI AAT'ATT

Cuts at:   1063    2232    2503    2835    4427    4926    5288    7305    9270 
   Size:       1169     271     332    1592     499     362    2017    1965

Cuts at:   9270    9396    9920   10458   12957    1063 
   Size:        126     524     538    2499    4140

  Fragments arranged by size: 

                4140    2499    2017    1965    1592    1169     538     524
                 499     362     332     271     126
 
StuI AGG'CCT

Cuts at:   9878    9878 
   Size:      16034
 
StyI C'CwwG_G

Cuts at:    107    1045    3756    3766    6937   11174   11369   13168   13522 
   Size:        938    2711      10    3171    4237     195    1799     354

Cuts at:  13522     107 
   Size:       2619

  Fragments arranged by size: 

                4237    3171    2711    2619    1799     938     354     195
                  10
 
SwaI ATTT'AAAT

Cuts at:   3857    4133    3857 
   Size:        276   15758
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    978    2545    4143    9189    9372   13617   13750   14209   14418 
   Size:       1567    1598    5046     183    4245     133     459     209

Cuts at:  14418     978 
   Size:       2594

  Fragments arranged by size: 

                5046    4245    2594    1598    1567     459     209     183
                 133
 
TatI w'GTAC_w

Cuts at:   2366    2742    5355    7123    7175    7222    8259    9048    9355 
   Size:        376    2613    1768      52      47    1037     789     307

Cuts at:   9355    9841   10613   11139   11844   11859   12366   12438   15549 
   Size:        486     772     526     705      15     507      72    3111

Cuts at:  15549    2366 
   Size:       2851

  Fragments arranged by size: 

                3111    2851    2613    1768    1037     789     772     705
                 526     507     486     376     307      72      52      47
                  15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    596     628    2300    2332    5466    5498    6252    6284   12984 
   Size:         32    1672      32    3134      32     754      32    6700

Cuts at:  12984   13016   15551   15583     596 
   Size:         32    2535      32    1047

  Fragments arranged by size: 

                6700    3134    2535    1672    1047     754      32      32
                  32      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   8789    9798   13831    8789 
   Size:       1009    4033   10992

  Fragments arranged by size: 

               10992    4033    1009
 
XbaI T'CTAG_A

Cuts at:   3780    6430    6921    7296   10428   13065    3780 
   Size:       2650     491     375    3132    2637    6749

  Fragments arranged by size: 

                6749    3132    2650    2637     491     375
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3292    7615   13529    3292 
   Size:       4323    5914    5797

  Fragments arranged by size: 

                5914    5797    4323
 
XhoI C'TCGA_G

Cuts at:   1184    1184 
   Size:      16034
 
XmaI C'CCGG_G

Cuts at:  13054   13054 
   Size:      16034
 
XmnI GAAnn'nnTTC

Cuts at:    139     166    2521    2801    3515    5591    6356    7304    7451 
   Size:         27    2355     280     714    2076     765     948     147

Cuts at:   7451    8173    9214    9270    9302    9422    9548   10109   10424 
   Size:        722    1041      56      32     120     126     561     315

Cuts at:  10424   10856     139 
   Size:        432    5317

  Fragments arranged by size: 

                5317    2355    2076    1041     948     765     722     714
                 561     432     315     280     147     126     120      56
                  32      27
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PpiI     PsiI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     ApaI     AscI     BglI    BsiWI   BssHII     FseI    FspAI
     NotI     NruI     PasI     PmlI    PpuMI    PshAI   PspOMI    SacII
    SanDI     SfiI    SgrAI    SnaBI     SrfI     ZraI
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