Restriction map for pF1KA1944
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# NO CUT ENZYMES (   76 enzymes) #
##################################

Acc65I      AclI        AflII       AflIII      AgeI        
Aor51HI     AscI        AseI        AsnI        Asp718I     
AviII       AvrII       BciVI       BclI        BfaI        
BfrI        BglI        BlnI        BsaBI       BsiWI       
BsmI        Bsp1407I    BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstZ17I     CpoI        
DraI        DraIII      Eco47III    EcoRI       EcoT22I     
FbaI        FspI        HindIII     HpaI        KpnI        
MaeI        MamI        MfeI        MluI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SalI        SbfI        SexAI       SfiI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII         169  GACGTC               GACGT^C
AccI          412  GTCTAC               GT^[AC][GT]AC
AccIII        749  TCCGGA               T^CCGGA
AhdI           81  GACGGAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        688  GTGCAC               G^TGCAC
AocI          501  CCTGAGG              CC^T[ACGT]AGG
ApaI          781  GGGCCC               GGGCC^C
ApaLI         688  GTGCAC               G^TGCAC
Asp700I      2184  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI          81  GACGGAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         1123  GGCGCC               GGCGC^C
BbrPI        1197  CACGTG               CAC^GTG
BcgI         2981  GCATGGATTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1250  AGATCT               A^GATCT
BsaAI        1197  CACGTG               [CT]AC^GT[GA]
BseAI         749  TCCGGA               T^CCGGA
BspEI         749  TCCGGA               T^CCGGA
BsrBI        1684  GAGCGG               CCGCTC(-3/-3)
BstAPI        309  GCAAGTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        501  CCTGAGG              CC^T[ACGT]AGG
EagI         1760  CGGCCG               C^GGCCG
Eam1105I       81  GACGGAAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1760  CGGCCG               C^GGCCG
Eco52I       1760  CGGCCG               C^GGCCG
Eco81I        501  CCTGAGG              CC^T[ACGT]AGG
EcoRV         202  GATATC               GAT^ATC
FseI         1761  GGCCGGCC             GGCCGG^CC
HincII       1498  GTCAAC               GT[CT]^[GA]AC
HindII       1498  GTCAAC               GT[CT]^[GA]AC
KasI         1123  GGCGCC               G^GCGCC
KspI         1695  CCGCGG               CCGC^GG
MroI          749  TCCGGA               T^CCGGA
NarI         1123  GGCGCC               GG^CGCC
NcoI         1724  CCATGG               C^CATGG
PmaCI        1197  CACGTG               CAC^GTG
PmlI         1197  CACGTG               CAC^GTG
SacII        1695  CCGCGG               CCGC^GG
SapI         1162  GAAGAGC              GCTCTTC(1/4)
ScaI         2519  AGTACT               AGT^ACT
SfoI         1123  GGCGCC               GGC^GCC
SmaI          230  CCCGGG               CCC^GGG
SmlI          100  CTTGAG               C^T[CT][GA]AG
StuI         1648  AGGCCT               AGG^CCT
XmaCI         230  CCCGGG               C^CCGGG
XmaI          230  CCCGGG               C^CCGGG
XmnI         2184  GAAAGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcyI          169  GACGTC               G[GA]^CG[CT]C
             1123  GGCGCC              
BamHI          95  GGATCC               G^GATCC
              746  GGATCC              
BbvCI        3009  CCTCAGC              CCTCAGC(-5/-2)
             1753  GCTGAGG             
BlpI         2043  GCTCAGC              GC^T[ACGT]AGC
             2736  GCTGAGC             
Bpu1102I     2043  GCTCAGC              GC^T[ACGT]AGC
             2736  GCTGAGC             
BsaHI         169  GACGTC               G[GA]^CG[CT]C
             1123  GGCGCC              
BsaWI         749  TCCGGA               [AT]^CCGG[AT]
             2906  ACCGGA              
BsiEI         471  CGACCG               CG[GA][CT]^CG
             1760  CGGCCG              
BspDI         769  ATCGAT               AT^CGAT
             1147  ATCGAT              
BspHI         206  TCATGA               T^CATGA
             2883  TCATGA              
BstEII       1171  GGTGACC              G^GT[ACGT]ACC
             1986  GGTGACC             
BstPI        1171  GGTGACC              G^GT[ACGT]ACC
             1986  GGTGACC             
CelII        2043  GCTCAGC              GC^T[ACGT]AGC
             2736  GCTGAGC             
ClaI          769  ATCGAT               AT^CGAT
             1147  ATCGAT              
DrdI         2019  GACAGGGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2568  GACAGACAGGTC        
EcoO65I      1171  GGTGACC              G^GT[ACGT]ACC
             1986  GGTGACC             
EcoT14I      1724  CCATGG               C^C[AT][AT]GG
             2165  CCTTGG              
HaeII         478  AGCGCC               [GA]GCGC^[CT]
             1123  GGCGCC              
Hin1I         169  GACGTC               G[GA]^CG[CT]C
             1123  GGCGCC              
RcaI          206  TCATGA               T^CATGA
             2883  TCATGA              
StyI         1724  CCATGG               C^C[AT][AT]GG
             2165  CCTTGG              


#########################################
# 3 OR MORE CUT ENZYMES (  115 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AspI        
AvaI        AvaII       BalI        BanI        BanII       
BbsI        BbvI        BcnI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrDI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
PflFI       PflMI       PleI        PpuMI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XcmI        XhoII       
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