Restriction map for pF1KA1944
 (Linear) (Six-base) MAPSORT of: KIAA1944.seq  Check: 5922  from: 1  to: 3297



KIAA1944.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1796    1833    3297 
   Size:       1796      37    1464

  Fragments arranged by size: 

                1796    1464      37
 
AatII G_ACGT'C

Cuts at:      0     173    3297 
   Size:        173    3124
 
AccI GT'mk_AC

Cuts at:      0     413    3297 
   Size:        413    2884
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     968    3297 
   Size:        205     763    2329

  Fragments arranged by size: 

                2329     763     205
 
AhdI GACnn_n'nnGTC

Cuts at:      0      86    3297 
   Size:         86    3211
 
AleI CACnn'nnGTG

Cuts at:      0    2080    3297 
   Size:       2080    1217
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2981    3277    3297 
   Size:       2981     296      20

  Fragments arranged by size: 

                2981     296      20
 
AlwNI CAG_nnn'CTG

Cuts at:      0     441     464    1530    1887    1926    2132    3237    3297 
   Size:        441      23    1066     357      39     206    1105      60

  Fragments arranged by size: 

                1105    1066     441     357     206      60      39      23
            
 
ApaI G_GGCC'C

Cuts at:      0     785    3297 
   Size:        785    2512
 
ApaLI G'TGCA_C

Cuts at:      0     688    3297 
   Size:        688    2609
 
ApoI r'AATT_y

Cuts at:      0     364     939    2248    2386    3136    3297 
   Size:        364     575    1309     138     750     161

  Fragments arranged by size: 

                1309     750     575     364     161     138
 
AvaI C'yCGr_G

Cuts at:      0     230     527    2002    3297 
   Size:        230     297    1475    1295

  Fragments arranged by size: 

                1475    1295     297     230
 
BamHI G'GATC_C

Cuts at:      0      95     746    3297 
   Size:         95     651    2551

  Fragments arranged by size: 

                2551     651      95
 
BanI G'GyrC_C

Cuts at:      0      29     315    1123    1560    2290    2560    3297 
   Size:         29     286     808     437     730     270     737

  Fragments arranged by size: 

                 808     737     730     437     286     270      29
 
BanII G_rGCy'C

Cuts at:      0     593     677     785    1264    1604    1798    1856    3101 
   Size:        593      84     108     479     340     194      58    1245

Cuts at:   3101    3297 
   Size:        196

  Fragments arranged by size: 

                1245     593     479     340     196     194     108      84
                  58
 
BbeI G_GCGC'C

Cuts at:      0    1127    3297 
   Size:       1127    2170
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     731    1342    1436    2769    2790    3297 
   Size:        731     611      94    1333      21     507

  Fragments arranged by size: 

                1333     731     611     507      94      21
 
BbvCI CC'TCA_GC

Cuts at:      0    1754    3010    3297 
   Size:       1754    1256     287

  Fragments arranged by size: 

                1754    1256     287
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2970    3004    3297 
   Size:       2970      34     293

  Fragments arranged by size: 

                2970     293      34
 
BglII A'GATC_T

Cuts at:      0    1250    3297 
   Size:       1250    2047
 
BlpI GC'TnA_GC

Cuts at:      0    2044    2737    3297 
   Size:       2044     693     560

  Fragments arranged by size: 

                2044     693     560
 
Bme1580I G_kGCm'C

Cuts at:      0       7     320     692     785    1565    1631    2563    3297 
   Size:          7     313     372      93     780      66     932     734

  Fragments arranged by size: 

                 932     780     734     372     313      93      66       7
            
 
BmgBI CAC'GTC

Cuts at:      0     909    1229    1434    3297 
   Size:        909     320     205    1863

  Fragments arranged by size: 

                1863     909     320     205
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     474    2898    3297 
   Size:        474    2424     399

  Fragments arranged by size: 

                2424     474     399
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2897    3297 
   Size:       2897     400
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     607     918    1420    1564    1623    3297 
   Size:        201     406     311     502     144      59    1674

  Fragments arranged by size: 

                1674     502     406     311     201     144      59
 
Bpu10I CC'TnA_GC

Cuts at:      0     418    1531    1754    2087    3010    3297 
   Size:        418    1113     223     333     923     287

  Fragments arranged by size: 

                1113     923     418     333     287     223
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121    3297 
   Size:        121    3176
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     517    1368    1593    3297 
   Size:        517     851     225    1704

  Fragments arranged by size: 

                1704     851     517     225
 
BsaAI yAC'GTr

Cuts at:      0    1199    3297 
   Size:       1199    2098
 
BsaHI Gr'CG_yC

Cuts at:      0     170    1124    3297 
   Size:        170     954    2173

  Fragments arranged by size: 

                2173     954     170
 
BsaWI w'CCGG_w

Cuts at:      0     749    2906    3297 
   Size:        749    2157     391

  Fragments arranged by size: 

                2157     749     391
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     580     610    2926    2956    3297 
   Size:        580      30    2316      30     341

  Fragments arranged by size: 

                2316     580     341      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     648     747    1684    1687    1864    1922    2872    2934 
   Size:        648      99     937       3     177      58     950      62

Cuts at:   2934    2998    3150    3297 
   Size:         64     152     147

  Fragments arranged by size: 

                 950     937     648     177     152     147      99      64
                  62      58       3
 
BseYI C'CCAG_C

Cuts at:      0    1791    2132    2644    2664    3297 
   Size:       1791     341     512      20     633

  Fragments arranged by size: 

                1791     633     512     341      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     454    1686    2029    3247    3297 
   Size:        454    1232     343    1218      50

  Fragments arranged by size: 

                1232    1218     454     343      50
 
BsiEI CG_ry'CG

Cuts at:      0     474    1763    3297 
   Size:        474    1289    1534

  Fragments arranged by size: 

                1534    1289     474
 
BsiHKAI G_wGCw'C

Cuts at:      0     593     677     692    1604    3297 
   Size:        593      84      15     912    1693

  Fragments arranged by size: 

                1693     912     593      84      15
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     177     899    1641    3162    3297 
   Size:        177     722     742    1521     135

  Fragments arranged by size: 

                1521     742     722     177     135
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     320     593     677     692     785    1264    1565 
   Size:          7     313     273      84      15      93     479     301

Cuts at:   1565    1604    1631    1798    1856    2563    3101    3297 
   Size:         39      27     167      58     707     538     196

  Fragments arranged by size: 

                 707     538     479     313     301     273     196     167
                  93      84      58      39      27      15       7
 
BspEI T'CCGG_A

Cuts at:      0     749    3297 
   Size:        749    2548
 
BspHI T'CATG_A

Cuts at:      0     206    2883    3297 
   Size:        206    2677     414

  Fragments arranged by size: 

                2677     414     206
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     426    1184    1194    1796    1833    3297 
   Size:        426     758      10     602      37    1464

  Fragments arranged by size: 

                1464     758     602     426      37      10
 
BsrBI CCG'CTC

Cuts at:      0    1686    3297 
   Size:       1686    1611
 
BsrDI GCAATG_nn'

Cuts at:      0     747    2264    2697    3297 
   Size:        747    1517     433     600

  Fragments arranged by size: 

                1517     747     600     433
 
BsrFI r'CCGG_y

Cuts at:      0      42     422     548    1762    3297 
   Size:         42     380     126    1214    1535

  Fragments arranged by size: 

                1535    1214     380     126      42
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     315    3297 
   Size:        315    2982
 
BstEII G'GTnAC_C

Cuts at:      0    1171    1986    3297 
   Size:       1171     815    1311

  Fragments arranged by size: 

                1311    1171     815
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1191    2082    2211    3297 
   Size:       1191     891     129    1086

  Fragments arranged by size: 

                1191    1086     891     129
 
BstYI r'GATC_y

Cuts at:      0      95     746    1250    1883    1922    3234    3297 
   Size:         95     651     504     633      39    1312      63

  Fragments arranged by size: 

                1312     651     633     504      95      63      39
 
Bsu36I CC'TnA_GG

Cuts at:      0     502    3297 
   Size:        502    2795
 
BtgI C'CryG_G

Cuts at:      0     617     644     668    1367    1695    1724    3297 
   Size:        617      27      24     699     328      29    1573

  Fragments arranged by size: 

                1573     699     617     328      29      27      24
 
BtsI GCAGTG_nn'

Cuts at:      0    2075    2087    2963    3297 
   Size:       2075      12     876     334

  Fragments arranged by size: 

                2075     876     334      12
 
ClaI AT'CG_AT

Cuts at:      0     770    1148    3297 
   Size:        770     378    2149

  Fragments arranged by size: 

                2149     770     378
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     606     641    3297 
   Size:        606      35    2656

  Fragments arranged by size: 

                2656     606      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2025    2574    3297 
   Size:       2025     549     723

  Fragments arranged by size: 

                2025     723     549
 
EaeI y'GGCC_r

Cuts at:      0      59     578     869    1343    1760    2201    2391    2786 
   Size:         59     519     291     474     417     441     190     395

Cuts at:   2786    3297 
   Size:        511

  Fragments arranged by size: 

                 519     511     474     441     417     395     291     190
                  59
 
EagI C'GGCC_G

Cuts at:      0    1760    3297 
   Size:       1760    1537
 
EarI CTCTTCn'nnn_

Cuts at:      0    1157    1661    2341    3297 
   Size:       1157     504     680     956

  Fragments arranged by size: 

                1157     956     680     504
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1892    3297 
   Size:       1892    1405
 
EcoICRI GAG'CTC

Cuts at:      0     591     675    1602    3297 
   Size:        591      84     927    1695

  Fragments arranged by size: 

                1695     927     591      84
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     205     607     918     968    1420    1564    1623 
   Size:        201       4     402     311      50     452     144      59

Cuts at:   1623    3297 
   Size:       1674

  Fragments arranged by size: 

                1674     452     402     311     201     144      59      50
                   4
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     187     792    2299    2719    3120    3297 
   Size:        187     605    1507     420     401     177

  Fragments arranged by size: 

                1507     605     420     401     187     177
 
EcoO109I rG'GnC_Cy

Cuts at:      0     561     662     782    1734    3061    3297 
   Size:        561     101     120     952    1327     236

  Fragments arranged by size: 

                1327     952     561     236     120     101
 
EcoRV GAT'ATC

Cuts at:      0     204    3297 
   Size:        204    3093
 
FseI GG_CCGG'CC

Cuts at:      0    1766    3297 
   Size:       1766    1531
 
HaeII r_GCGC'y

Cuts at:      0     482    1127    3297 
   Size:        482     645    2170

  Fragments arranged by size: 

                2170     645     482
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      72     104     580     612    1111    1143    1213    1245 
   Size:         72      32     476      32     499      32      70      32

Cuts at:   1245    1402    1434    1616    1648    1914    1946    3053    3085 
   Size:        157      32     182      32     266      32    1107      32

Cuts at:   3085    3297 
   Size:        212

  Fragments arranged by size: 

                1107     499     476     266     212     182     157      72
                  70      32      32      32      32      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1500    3297 
   Size:       1500    1797
 
KasI G'GCGC_C

Cuts at:      0    1123    3297 
   Size:       1123    2174
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     365    1600    1616    2343    2900    3297 
   Size:        365    1235      16     727     557     397

  Fragments arranged by size: 

                1235     727     557     397     365      16
 
MscI TGG'CCA

Cuts at:      0     871    2203    2393    2788    3297 
   Size:        871    1332     190     395     509

  Fragments arranged by size: 

                1332     871     509     395     190
 
MslI CAynn'nnrTG

Cuts at:      0    1000    1843    1969    2080    2882    3196    3290    3297 
   Size:       1000     843     126     111     802     314      94       7

  Fragments arranged by size: 

                1000     843     802     314     126     111      94       7
            
 
MspA1I CmG'CkG

Cuts at:      0     545     602    1072    1099    1191    1290    1575    1697 
   Size:        545      57     470      27      92      99     285     122

Cuts at:   1697    2013    3045    3297 
   Size:        316    1032     252

  Fragments arranged by size: 

                1032     545     470     316     285     252     122      99
                  92      57      27
 
NaeI GCC'GGC

Cuts at:      0     424     550    1764    3297 
   Size:        424     126    1214    1533

  Fragments arranged by size: 

                1533    1214     424     126
 
NarI GG'CG_CC

Cuts at:      0    1124    3297 
   Size:       1124    2173
 
NcoI C'CATG_G

Cuts at:      0    1724    3297 
   Size:       1724    1573
 
NgoMIV G'CCGG_C

Cuts at:      0     422     548    1762    3297 
   Size:        422     126    1214    1535

  Fragments arranged by size: 

                1535    1214     422     126
 
PasI CC'CwG_GG

Cuts at:      0     694    1768    3244    3297 
   Size:        694    1074    1476      53

  Fragments arranged by size: 

                1476    1074     694      53
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     453    1791    3237    3297 
   Size:        453    1338    1446      60

  Fragments arranged by size: 

                1446    1338     453      60
 
PfoI T'CCnGG_A

Cuts at:      0     259    3297 
   Size:        259    3038
 
PmlI CAC'GTG

Cuts at:      0    1199    3297 
   Size:       1199    2098
 
PpuMI rG'GwC_Cy

Cuts at:      0     561     662    1734    3061    3297 
   Size:        561     101    1072    1327     236

  Fragments arranged by size: 

                1327    1072     561     236     101
 
PspOMI G'GGCC_C

Cuts at:      0     781    3297 
   Size:        781    2516
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1504    1536    3297 
   Size:       1504      32    1761

  Fragments arranged by size: 

                1761    1504      32
 
PstI C_TGCA'G

Cuts at:      0     227     557    1580    1712    2082    2102    2588    3297 
   Size:        227     330    1023     132     370      20     486     709

  Fragments arranged by size: 

                1023     709     486     370     330     227     132      20
            
 
PvuII CAG'CTG

Cuts at:      0     545     602    1072    1099    1191    2013    3045    3297 
   Size:        545      57     470      27      92     822    1032     252

  Fragments arranged by size: 

                1032     822     545     470     252      92      57      27
            
 
SacI G_AGCT'C

Cuts at:      0     593     677    1604    3297 
   Size:        593      84     927    1693

  Fragments arranged by size: 

                1693     927     593      84
 
SacII CC_GC'GG

Cuts at:      0    1698    3297 
   Size:       1698    1599
 
SanDI GG'GwC_CC

Cuts at:      0     662    3061    3297 
   Size:        662    2399     236

  Fragments arranged by size: 

                2399     662     236
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1157    3297 
   Size:       1157    2140
 
ScaI AGT'ACT

Cuts at:      0    2521    3297 
   Size:       2521     776
 
SfcI C'TryA_G

Cuts at:      0     223     553    1576    1708    2078    2098    2584    3297 
   Size:        223     330    1023     132     370      20     486     713

  Fragments arranged by size: 

                1023     713     486     370     330     223     132      20
            
 
SfoI GGC'GCC

Cuts at:      0    1125    3297 
   Size:       1125    2172
 
SmaI CCC'GGG

Cuts at:      0     232    3297 
   Size:        232    3065
 
SmlI C'TyrA_G

Cuts at:      0     100    3297 
   Size:        100    3197
 
StuI AGG'CCT

Cuts at:      0    1650    3297 
   Size:       1650    1647
 
StyI C'CwwG_G

Cuts at:      0    1724    2165    3297 
   Size:       1724     441    1132

  Fragments arranged by size: 

                1724    1132     441
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     707    1548    3297 
   Size:        707     841    1749

  Fragments arranged by size: 

                1749     841     707
 
TatI w'GTAC_w

Cuts at:      0    2519    3297 
   Size:       2519     778
 
Tth111I GACn'n_nGTC

Cuts at:      0     409     715    1462    3297 
   Size:        409     306     747    1835

  Fragments arranged by size: 

                1835     747     409     306
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     309    1971    2146    3297 
   Size:        309    1662     175    1151

  Fragments arranged by size: 

                1662    1151     309     175
 
XmaI C'CCGG_G

Cuts at:      0     230    3297 
   Size:        230    3067
 
XmnI GAAnn'nnTTC

Cuts at:      0    2188    3297 
   Size:       2188    1109
 
ZraI GAC'GTC

Cuts at:      0     171    3297 
   Size:        171    3126
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AhdI     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI     BtsI     ClaI    CspCI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRV     FseI    HaeII    Hin4I   HincII     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     PasI    PflMI
     PfoI     PmlI    PpuMI   PspOMI     PsrI     PstI    PvuII     SacI
    SacII    SanDI     SapI     ScaI     SfcI     SfoI     SmaI     SmlI
     StuI     StyI    TaqII     TatI  Tth111I     XcmI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI    BciVI     BclI    BfrBI     BglI
     BmtI    BsaBI    BsiWI     BsmI    BsrGI   BssHII    BssSI    BstBI
  BstZ17I    BtgZI     DraI   DraIII    EcoRI     FalI     FspI    FspAI
  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI     PmeI     PpiI    PshAI
     PsiI    PspXI     PvuI    RsrII     SalI     SbfI    SexAI     SfiI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
     TstI     XbaI     XhoI
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