Restriction map for pF1KA1700
 (Linear) (Six-base) MAPSORT of: KIAA1700.seq  Check: 2137  from: 1  to: 1995



KIAA1700.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:55  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     355     365    1995 
   Size:        355      10    1630

  Fragments arranged by size: 

                1630     355      10
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281    1412    1995 
   Size:        281    1131     583

  Fragments arranged by size: 

                1131     583     281
 
AflII C'TTAA_G

Cuts at:      0    1606    1995 
   Size:       1606     389
 
AflIII A'CryG_T

Cuts at:      0     791    1995 
   Size:        791    1204
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1465    1774    1995 
   Size:       1465     309     221

  Fragments arranged by size: 

                1465     309     221
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1667    1880    1995 
   Size:       1667     213     115

  Fragments arranged by size: 

                1667     213     115
 
ApaLI G'TGCA_C

Cuts at:      0     724    1995 
   Size:        724    1271
 
ApoI r'AATT_y

Cuts at:      0     482     653    1891    1995 
   Size:        482     171    1238     104

  Fragments arranged by size: 

                1238     482     171     104
 
AseI AT'TA_AT

Cuts at:      0     137    1995 
   Size:        137    1858
 
AvaI C'yCGr_G

Cuts at:      0     604    1995 
   Size:        604    1391
 
BanI G'GyrC_C

Cuts at:      0    1477    1995 
   Size:       1477     518
 
BanII G_rGCy'C

Cuts at:      0     203    1852    1995 
   Size:        203    1649     143

  Fragments arranged by size: 

                1649     203     143
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1178    1995 
   Size:       1178     817
 
BbvCI CC'TCA_GC

Cuts at:      0    1707    1995 
   Size:       1707     288
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1237    1610    1995 
   Size:       1237     373     385

  Fragments arranged by size: 

                1237     385     373
 
Bme1580I G_kGCm'C

Cuts at:      0     728    1082    1091    1995 
   Size:        728     354       9     904

  Fragments arranged by size: 

                 904     728     354       9
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     538    1995 
   Size:        538    1457
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     926     967    1995 
   Size:        352     574      41    1028

  Fragments arranged by size: 

                1028     574     352      41
 
Bpu10I CC'TnA_GC

Cuts at:      0    1707    1995 
   Size:       1707     288
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     916    1171    1995 
   Size:        916     255     824

  Fragments arranged by size: 

                 916     824     255
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1766    1995 
   Size:       1766     229
 
BsaHI Gr'CG_yC

Cuts at:      0    1003    1995 
   Size:       1003     992
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     828     858    1763    1793    1995 
   Size:        828      30     905      30     202

  Fragments arranged by size: 

                 905     828     202      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1251    1995 
   Size:       1251     744
 
BseYI C'CCAG_C

Cuts at:      0     736    1081    1090    1935    1995 
   Size:        736     345       9     845      60

  Fragments arranged by size: 

                 845     736     345      60       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     925    1117    1132    1995 
   Size:        925     192      15     863

  Fragments arranged by size: 

                 925     863     192      15
 
BsiHKAI G_wGCw'C

Cuts at:      0     203     728    1995 
   Size:        203     525    1267

  Fragments arranged by size: 

                1267     525     203
 
BsmI GAATG_Cn'

Cuts at:      0     210    1452    1995 
   Size:        210    1242     543

  Fragments arranged by size: 

                1242     543     210
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     994    1995 
   Size:        994    1001
 
Bsp1286I G_dGCh'C

Cuts at:      0     203     728    1082    1091    1852    1995 
   Size:        203     525     354       9     761     143

  Fragments arranged by size: 

                 761     525     354     203     143       9
 
BspHI T'CATG_A

Cuts at:      0     776    1995 
   Size:        776    1219
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     355     365     442    1995 
   Size:        355      10      77    1553

  Fragments arranged by size: 

                1553     355      77      10
 
BsrBI CCG'CTC

Cuts at:      0     749    1995 
   Size:        749    1246
 
BsrFI r'CCGG_y

Cuts at:      0      92    1995 
   Size:         92    1903
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1880    1995 
   Size:       1880     115
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1305    1995 
   Size:       1305     690
 
BstYI r'GATC_y

Cuts at:      0     740    1995 
   Size:        740    1255
 
BtgI C'CryG_G

Cuts at:      0     661    1995 
   Size:        661    1334
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     744    1995 
   Size:        744    1251
 
BtsI GCAGTG_nn'

Cuts at:      0    1479    1504    1731    1995 
   Size:       1479      25     227     264

  Fragments arranged by size: 

                1479     264     227      25
 
DraIII CAC_nnn'GTG

Cuts at:      0    1021    1995 
   Size:       1021     974
 
EaeI y'GGCC_r

Cuts at:      0      94    1995 
   Size:         94    1901
 
EarI CTCTTCn'nnn_

Cuts at:      0     300     331     775    1838    1925    1995 
   Size:        300      31     444    1063      87      70

  Fragments arranged by size: 

                1063     444     300      87      70      31
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     741    1845    1995 
   Size:        741    1104     150

  Fragments arranged by size: 

                1104     741     150
 
EcoICRI GAG'CTC

Cuts at:      0     201    1995 
   Size:        201    1794
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     352     926     967    1412    1995 
   Size:        281      71     574      41     445     583

  Fragments arranged by size: 

                 583     574     445     281      71      41
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     526    1995 
   Size:        526    1469
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1490    1995 
   Size:       1490     505
 
EcoRI G'AATT_C

Cuts at:      0     482    1995 
   Size:        482    1513
 
EcoRV GAT'ATC

Cuts at:      0    1209    1626    1995 
   Size:       1209     417     369

  Fragments arranged by size: 

                1209     417     369
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     612    1212    1995 
   Size:        612     600     783

  Fragments arranged by size: 

                 783     612     600
 
FspI TGC'GCA

Cuts at:      0    1792    1995 
   Size:       1792     203
 
HaeII r_GCGC'y

Cuts at:      0    1138    1995 
   Size:       1138     857
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       6      38     256     271     288     303    1198    1230 
   Size:          6      32     218      15      17      15     895      32

Cuts at:   1230    1763    1795    1889    1906    1921    1938    1995 
   Size:        533      32      94      17      15      17      57

  Fragments arranged by size: 

                 895     533     218      94      57      32      32      32
                  17      17      17      15      15      15       6
 
HincII GTy'rAC

Cuts at:      0     231    1995 
   Size:        231    1764
 
HindIII A'AGCT_T

Cuts at:      0     154     806    1995 
   Size:        154     652    1189

  Fragments arranged by size: 

                1189     652     154
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     647    1995 
   Size:        647    1348
 
MslI CAynn'nnrTG

Cuts at:      0    1514    1995 
   Size:       1514     481
 
MspA1I CmG'CkG

Cuts at:      0    1125    1667    1748    1758    1995 
   Size:       1125     542      81      10     237

  Fragments arranged by size: 

                1125     542     237      81      10
 
NaeI GCC'GGC

Cuts at:      0      94    1995 
   Size:         94    1901
 
NgoMIV G'CCGG_C

Cuts at:      0      92    1995 
   Size:         92    1903
 
NspI r_CATG'y

Cuts at:      0     795    1995 
   Size:        795    1200
 
PciI A'CATG_T

Cuts at:      0     791    1995 
   Size:        791    1204
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     126     873    1667    1995 
   Size:        126     747     794     328

  Fragments arranged by size: 

                 794     747     328     126
 
PfoI T'CCnGG_A

Cuts at:      0     872    1995 
   Size:        872    1123
 
PpuMI rG'GwC_Cy

Cuts at:      0    1490    1995 
   Size:       1490     505
 
PstI C_TGCA'G

Cuts at:      0    1415    1753    1995 
   Size:       1415     338     242

  Fragments arranged by size: 

                1415     338     242
 
PvuII CAG'CTG

Cuts at:      0    1667    1748    1758    1995 
   Size:       1667      81      10     237

  Fragments arranged by size: 

                1667     237      81      10
 
SacI G_AGCT'C

Cuts at:      0     203    1995 
   Size:        203    1792
 
SapI GCTCTTCn'nnn_

Cuts at:      0     331    1925    1995 
   Size:        331    1594      70

  Fragments arranged by size: 

                1594     331      70
 
SfcI C'TryA_G

Cuts at:      0    1411    1743    1749    1995 
   Size:       1411     332       6     246

  Fragments arranged by size: 

                1411     332     246       6
 
SmlI C'TyrA_G

Cuts at:      0     931    1186    1606    1995 
   Size:        931     255     420     389

  Fragments arranged by size: 

                 931     420     389     255
 
StuI AGG'CCT

Cuts at:      0    1425    1995 
   Size:       1425     570
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1446    1995 
   Size:       1446     549
 
TatI w'GTAC_w

Cuts at:      0     315    1995 
   Size:        315    1680
 
Tth111I GACn'n_nGTC

Cuts at:      0    1777    1995 
   Size:       1777     218
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      13    1891    1995 
   Size:         13    1878     104

  Fragments arranged by size: 

                1878     104      13
 
XmnI GAAnn'nnTTC

Cuts at:      0     340    1995 
   Size:        340    1655
 

 Enzymes that do cut:

     AarI     AcuI    AflII   AflIII     AlfI    AlwNI    ApaLI     ApoI
     AseI     AvaI     BanI    BanII     BbsI    BbvCI     BglI Bme1580I
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrFI   BstAPI    BstXI    BstYI     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII
     MmeI     MslI   MspA1I     NaeI   NgoMIV     NspI     PciI    PflMI
     PfoI    PpuMI     PstI    PvuII     SacI     SapI     SfcI     SmlI
     StuI    TaqII     TatI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI     AleI
     AloI     ApaI     AscI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI    BspEI    BsrDI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     ClaI
    CspCI     DraI     DrdI     EagI     FseI    FspAI     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI
     SwaI     TstI     XbaI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com